LOCUS       Exported               12198 bp ds-DNA     circular SYN 19-NOV-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       Exported                4570 bp ds-DNA    circular SYN 
            03-FEB-2014
            DEFINITION  .
            ACCESSION   .
            VERSION     .
            KEYWORDS    SM33 - AAVS1-MCS donor
            SOURCE      synthetic DNA construct
              ORGANISM  synthetic DNA construct
            REFERENCE   1  (bases 1 to 4570)
              AUTHORS   .
              TITLE     Direct Submission
              JOURNAL   Exported Monday, Feb 03, 2014 from SnapGene 2.2.2
                        http://www.snapgene.com
            COMMENT     This file is created by Vector NTI
                        http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    625..1368
                     /label=AAVS L homology
     exon            725..1102
                     /label=exon1
     misc_feature    1255..1368
                     /label=AAVS integration site
     CDS             1379..2052
                     /label=A2UOCE
     exon            1483..1833
                     /label=CBX3 exon 1
                     /note="CBX3 exon 1"
     variation       1834
                     /label=mutant splice donor
                     /note="mutant splice donor"
     variation       1872
                     /label=mutant cryptic splice acceptor
                     /note="mutant cryptic splice acceptor"
     exon            1976..2051
                     /label=CBX3 alt exon 1
                     /note="CBX3 alt exon 1"
     promoter        2054..3232
                     /label=hEf1a
     intron          2285..3223
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
     primer_bind     3249..3268
                     /label=T7
     promoter        3249..3267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3364..4602
                     /codon_start=1
                     /label=iCasp9
                     /translation="MLEGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDR
                     NKPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDV
                     ELLKLESGGGSGVDGFGDVGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRT
                     RTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELARQDHGALDCCVVVILSHG
                     CQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFE
                     VASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRD
                     PKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKT
                     SVDYPYDVPDYALD"
     CDS             4570..4596
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     transit_peptide 4603..4668
                     /label=P2A
     CDS             4669..5988
                     /codon_start=1
                     /label=Crry
                     /translation="MEVSSRSSEPLDPVWLLVAFGRGGVKLEVLLLFLLPFTLGHCPAP
                     SQLPSAKPINLTDESMFPIGTYLLYECLPGYIKRQFSITCKQDSTWTSAEDKCIRKQCK
                     TPSDPENGLVHVHTGIQFGSRINYTCNQGYRLIGSSSAVCVITDQSVDWDTEAPICEWI
                     PCEIPPGIPNGDFFSSTREDFHYGMVVTYRCNTDARGKALFNLVGEPSLYCTSNDGEIG
                     VWSGPPPQCIELNKCTPPPYVENAVMLSENRSLFSLRDIVEFRCHPGFIMKGASSVHCQ
                     SLNKWEPELPSCFKGVICRLPQEMSGFQKGLGMKKEYYYGENVTLECEDGYTLEGSSQS
                     QCQSDGSWNPLLAKCVSRSISGLIVGIFIGIIVFILVIIVFIWMILKYKKRNTTDEKYK
                     EVGIHLNYKEDSCVRLQSLLTSQENSSTTSPARNSLTQEVS"
     CDS             complement(5296..5307)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     transit_peptide 5989..6051
                     /label=T2A
     CDS             6052..6420
                     /codon_start=1
                     /label=CD59
                     /translation="MRAQRGLILLLLLLAVFCSTAVSLTCYHCFQPVVSSCNMNSTCSP
                     DQDSCLYAVAGMQVYQRCWKQSDCHGEIIMDQLEETKLKFRCCQFNLCNKSDGSLGKTP
                     LLGTSVLVAILNLCFLSHL"
     transit_peptide 6421..6489
                     /label=E2A
     sig_peptide     6490..6549
     misc_feature    6550..6576
                     /label=Qdm peptide
     misc_feature    6577..6621
                     /label=Linker
     CDS             6622..6918
                     /codon_start=1
                     /label=beta 2 microglobulin
                     /translation="IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKK
                     IPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDM"
     CDS             6979..7989
                     /codon_start=1
                     /label=mature peptide
                     /translation="SPHSLRYFTTAVSRPGLGEPRFIIVGYVDDTQFVRFDSDAENPRM
                     EPRARWIEQEGPEYWERETWKARDMGRNFRVNLRTLLGYYNQSNDESHTLQWMYGCDVG
                     PDGRLLRGYCQEAYDGQDYISLNEDLRSWTANDIASQISKHKSEAVDEAHQQRAYLQGP
                     CVEWLHRYLRLGNETLQRSDPPKAHVTHHPRSEDEVTLRCWALGFYPADITLTWQLNGE
                     ELTQDMELVETRPAGDGTFQKWAAVVVPLGKEQYYTCHVYHEGLPEPLTLRWEPPPSTV
                     SNMVIIAVLVVLGAVIILGAVVAFVMKRRRHIGVKGCYAHVLGSKSFQTSDWPQKA"
     transit_peptide 7990..8064
                     /label=F2A
     CDS             8065..8859
                     /codon_start=1
                     /label=neomycin resistance
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    8901..9175
                     /label=BGH polyA
     misc_feature    9195..9994
                     /label=AAVS R homology
     primer_bind     complement(10049..10065)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10073..10089
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10097..10127)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10142..10163
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10451..11039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    11213..12070
                     /label=Ampicillin resistance
     promoter        complement(12071..12175)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tcgcgccgtg acgtcagcac gccgggcggg gaccgggaga
      661 tccttggggc ggtggggggc cagcggcagt tcccggcggc ccccggggcg ggcgggcggg
      721 cgggtggtgg cggcggttgg ggctcggcgc tcgctcgctc gctgggcggg cgggcggtgc
      781 gatgtccgga gaggatggcc cggcggctgg cccgggggcg gcggcggcgg ctgcccggga
      841 gcggcgacgg gagcagctgc ggcagtgggg ggcgcgggcg ggcgccgagc ctggccccgg
      901 agagcgccgc gcccgcaccg tccgcttcga gcgcgccgcc gagttcctgg cggcctgtgc
      961 gggcggcgac ctggacgagg cgcgtctgat gctgcgcgcc gccgaccctg gccccggcgc
     1021 cgagctcgac cccgccgcgc cgccgcccgc ccgcgccgtg ctggactcca ccaacgccga
     1081 cggtatcagc gccctgcacc aggtcagcgc cccccgcccg gcgtctcccg gggccaggtc
     1141 caccctctgc tgcgccacct ggggcatcct ccttccccgt tgccagtctc gatccgcccc
     1201 gtcgttcctg gccctgggct ttgccaccct atgctgacac cccgtcccag tcccccttac
     1261 cattcccctt cgaccacccc acttccgaat tggagccgct tcaactggcc ctgggcttag
     1321 ccactctgtg ctgaccactc tgccccaggc ctccttacca ttccccttaa acaccggtgg
     1381 gaggtggtcc ctgctgttac gccaatgata acccccgcca gaaaaatctt agtagccttc
     1441 cctttttgtt ttccgtgccc caactcggcg gattgactcg gccccttccg gaaacacccg
     1501 aatcaacttc tagtcaaatt attgttcacg ccgcaatgac ccacccctgg cccgcgtctg
     1561 tggaactgac ccctggtgta caggagagtt cgctgctgaa agtggtccca aaggggtact
     1621 agtttttaag ctcccaactc cccctccccc agcgtctgga ggattccaca ccctcgcacc
     1681 gcaggggcga ggaagtgggc ggagtccggt tttggcgcca gccgctgagg ctgccaagca
     1741 gaaaagccac cgctgaggag actccggtca ctgtcctcgc cccgcctccc ccttccctcc
     1801 ccttggggac caccgggcgc cacgccgcga acgttaagtg ccgcggtcgt cggcgcctcc
     1861 gccctccccc ttgggcccca attcccagcg ggcgcggcgc gcggcccctc cccccgccgg
     1921 gcgcgcgccc gctgccccgc ccttcgtggc cgcccggcgt gggcggtgcc acccctcccc
     1981 ccggcggccc cgcgcgcagc tcccggctcc ctcccccttc ggatgtggct tgagctgtag
     2041 gcgcggaggg ccgggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     2101 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     2161 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     2221 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     2281 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     2341 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     2401 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     2461 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     2521 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     2581 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     2641 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     2701 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     2761 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     2821 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     2881 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     2941 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     3001 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     3061 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     3121 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     3181 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattag
     3241 cttggtacta atacgactca ctatagggag acccaagctg gctaggtaag cttggtaccg
     3301 agctcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc
     3361 gccatgctgg agggcgtgca ggtcgagaca atcagccctg gagacggaag gaccttcccc
     3421 aaaagaggcc aaacatgcgt ggtccattac accggaatgc tcgaggacgg caaaaaggtg
     3481 gacagcagca gggataggaa caaacccttc aaattcatgc tgggaaaaca agaggtgatc
     3541 agaggatggg aggaaggagt ggcccaaatg tccgtgggac agagggccaa gctgacaatc
     3601 tcccccgatt acgcctacgg cgctacaggc catcctggaa tcatcccccc ccacgccaca
     3661 ctggtgttcg atgtggaact cctcaagctg gagtccggag gaggctccgg agtggacgga
     3721 tttggcgacg tgggagctct ggaaagcctc agaggaaacg ccgacctcgc ctatattctg
     3781 tccatggagc cctgtggcca ttgcctcatc attaataacg tgaacttctg cagggaaagc
     3841 ggactgagga caagaaccgg cagcaacatt gactgtgaga aactgagaag gagattcagc
     3901 tccctgcatt tcatggtgga ggtgaagggc gatctgacag ccaagaaaat ggtcctggcc
     3961 ctgctcgagc tggccagaca ggaccacgga gctctggact gctgtgtggt cgtcatcctc
     4021 agccacggat gccaagctag ccatctgcaa ttccccggcg ctgtgtatgg aaccgatggc
     4081 tgtcccgtgt ccgtggaaaa aatcgtcaat atctttaacg gcacaagctg cccctccctc
     4141 ggaggcaaac ctaagctgtt ctttattcaa gcttgcggcg gcgagcaaaa ggaccatggc
     4201 tttgaagtgg cttccacctc ccccgaagac gagagccccg gatccaatcc tgagcccgat
     4261 gctaccccct tccaggaggg cctcaggacc ttcgaccagc tcgatgccat cagcagcctg
     4321 cctacccctt ccgacatctt cgtgtcctac agcacctttc ccggattcgt gagctggagg
     4381 gatcccaaga gcggaagctg gtatgtggag acactggacg atatttttga gcagtgggcc
     4441 cacagcgagg atctgcaatc cctgctgctg agagtcgcca acgccgtgag cgtgaaagga
     4501 atctacaagc agatgcccgg atgcttcaac ttcctgagga agaagctgtt cttcaagacc
     4561 agcgtggact atccctatga tgtgcccgac tacgccctcg atggctccgg agccaccaac
     4621 ttcagcctgc tcaagcaagc cggcgacgtg gaggagaacc ctggacctat ggaagtgtcc
     4681 tccagatcca gcgagcctct ggatcccgtg tggctcctgg tcgccttcgg aagaggaggc
     4741 gtcaagctgg aggtcctgct gctgtttctg ctccccttca cactgggcca ctgtcctgcc
     4801 cctagccaac tgccctccgc caagcctatc aacctcaccg atgagtccat gtttcccatt
     4861 ggcacctacc tcctgtatga gtgcctccct ggatatatta agagacagtt ctccatcaca
     4921 tgcaagcagg attccacctg gaccagcgcc gaagacaagt gcatcaggaa gcagtgcaaa
     4981 accccttccg atcccgaaaa tggactcgtg cacgtgcaca caggaatcca gttcggctcc
     5041 agaattaatt atacatgtaa tcagggctac agactgatcg gcagctccag cgctgtctgt
     5101 gtgattaccg atcagagcgt ggattgggac accgaggccc ctatctgtga gtggatccct
     5161 tgcgagattc cccctggcat tcctaacggc gacttcttta gctccaccag agaggatttc
     5221 cactacggca tggtcgtcac ctacaggtgc aacacagacg ccaggggaaa agccctgttc
     5281 aacctggtcg gcgagccttc cctctattgc acatccaatg acggcgaaat cggcgtctgg
     5341 agcggacctc ccccccaatg catcgaactg aacaagtgca cacccccccc ttatgtggag
     5401 aatgccgtca tgctcagcga aaataggtcc ctgttttccc tgagggacat cgtggagttc
     5461 aggtgtcacc ctggcttcat catgaaggga gccagcagcg tgcattgcca gagcctcaat
     5521 aagtgggagc ccgagctgcc ctcctgcttc aagggcgtca tttgcagact gccccaggag
     5581 atgagcggct tccaaaaggg cctcggcatg aagaaagagt actactatgg cgaaaatgtc
     5641 accctggaat gcgaggatgg ctatacactc gagggcagct cccagagcca gtgccagagc
     5701 gatggcagct ggaaccctct cctggctaag tgcgtgtcca ggagcatcag cggcctgatt
     5761 gtgggcatct ttattggaat tattgtcttc atcctcgtga tcattgtgtt tatctggatg
     5821 attctgaagt acaaaaaaag aaacaccaca gatgaaaagt ataaagaggt gggcattcat
     5881 ctcaactaca aggaggatag ctgcgtgaga ctgcagtccc tgctcacatc ccaagaaaat
     5941 agcagcacca catcccccgc cagaaattcc ctcacccaag aggtgtccgg aagcggcgag
     6001 ggaaggggaa gcctcctgac atgcggcgat gtggaagaaa atcccggccc tatgagggcc
     6061 cagaggggcc tgatcctgct gctcctcctg ctggccgtgt tctgcagcac agccgtgtcc
     6121 ctcacctgct accattgctt ccagcctgtc gtgtccagct gcaacatgaa tagcacatgc
     6181 tcccctgacc aggattcctg cctgtacgct gtcgctggaa tgcaggtgta ccagaggtgc
     6241 tggaagcaaa gcgactgtca tggcgagatc atcatggatc agctggagga gaccaagctg
     6301 aagttcagat gttgtcagtt taatctctgt aacaagagcg atggaagcct cggaaaaacc
     6361 cctctcctcg gaacaagcgt gctcgtcgcc attctgaatc tctgctttct gtcccatctg
     6421 ggatccggcc aatgcaccaa ctatgccctc ctcaaactcg ccggagatgt ggaatccaac
     6481 cccggcccta tggctaggtc cgtcaccctg gtcttcctgg tgctggtgag cctgaccggc
     6541 ctgtatgctg ctatggctcc caggacactc ctgctgggct gtggagcctc cggaggaggc
     6601 ggcagcggag gcggcggctc cattcagaag acacctcaaa tccaggtgta ctccagacac
     6661 ccccccgaga acggaaagcc taatattctc aattgttacg tgacccagtt tcaccccccc
     6721 catattgaga tccagatgct caaaaacggc aagaaaattc ctaaggtgga gatgagcgac
     6781 atgagcttta gcaaggactg gagcttctac attctcgccc acacagaatt tacacccacc
     6841 gagaccgaca cctatgcttg cagggtgaaa catgccagca tggctgagcc taagacagtg
     6901 tactgggata gggacatggg aggaggcgga tccggcggcg gcggaagcgg cggaggcggc
     6961 tccggcggcg gaggaagcag cccacactcg ctgcggtatt tcaccaccgc cgtgtcccgg
     7021 cccggcctcg gggagccccg gttcatcatt gtcggctacg tggacgacac gcagttcgtg
     7081 cgcttcgaca gcgacgcgga aaatccgagg atggagcctc gggcgcggtg gattgagcag
     7141 gaggggccgg agtattggga gcgggagact tggaaagcca gggacatggg gaggaacttc
     7201 agagtaaacc tgaggaccct gctcggctac tacaatcaga gtaacgacga atctcacacg
     7261 ctgcagtgga tgtacggctg cgacgtgggg cccgatgggc gcctgctccg cgggtattgt
     7321 caggaggcct acgatggcca ggattacatc tccctgaacg aggacctgcg ttcctggacc
     7381 gcgaatgaca tagcctcaca gatctctaag cacaagtcag aggcagtcga tgaggcccac
     7441 caacagaggg catacctgca aggtccttgc gtggagtggc tccatagata cctacggctg
     7501 ggaaatgaga cactgcagcg ctcagaccct ccaaaggcac atgtgaccca tcaccctaga
     7561 tctgaagatg aagtcaccct gaggtgctgg gccctgggct tctaccctgc tgacatcacc
     7621 ctgacctggc agttgaatgg ggaggagctg acccaggaca tggagcttgt ggagaccagg
     7681 cctgcagggg atggaacctt ccagaagtgg gcagctgtcg tggtgcctct tgggaaggag
     7741 cagtattaca catgccatgt gtaccatgag gggctgcctg agcccctcac cctgagatgg
     7801 gagcctcctc catccactgt ctccaacatg gtaatcatag ctgttctggt tgtccttgga
     7861 gctgtgatca tccttggagc tgtggtggct tttgtgatga agaggaggag acacataggt
     7921 gtaaaaggat gctatgctca tgttctaggc agcaagagct tccagacctc tgactggcct
     7981 cagaaggcag gcagcggagt gaagcagacc ctcaacttcg acctgctgaa gctggccgga
     8041 gacgtggaga gcaatcctgg acccatgatc gagcaggacg gactccatgc tggaagccct
     8101 gccgcttggg tcgagaggct ctttggatac gactgggctc agcagacaat cggctgcagc
     8161 gatgccgccg tgtttagact cagcgcccag ggcagacctg tgctgtttgt gaagaccgac
     8221 ctgagcggag ctctcaatga gctccaagat gaagccgcca gactgagctg gctcgccacc
     8281 acaggcgtgc cttgtgctgc cgtgctggac gtcgtcacag aggccggaag ggattggctg
     8341 ctcctgggcg aagtccccgg acaggatctg ctgagctccc atctggcccc tgccgaaaag
     8401 gtctccatta tggccgatgc catgaggagg ctgcacaccc tggatcccgc tacctgtccc
     8461 tttgaccatc aggccaagca caggattgag agggccagaa caagaatgga ggccggcctg
     8521 gtcgaccagg atgatctgga cgaggaacac cagggcctcg ctcctgccga gctctttgcc
     8581 agactcaagg ccaggatgcc tgacggcgaa gatctggtgg tgacccatgg agatgcctgt
     8641 ctccccaaca tcatggtgga aaacggcagg ttctccggat tcattgactg cggcaggctc
     8701 ggcgtggccg acaggtatca ggacatcgcc ctggctacca gagatatcgc tgaggagctg
     8761 ggaggagagt gggccgatag atttctcgtg ctctatggca ttgccgctcc cgacagccag
     8821 agaattgcct tctacagact gctcgatgag tttttttgag tttaaacccg ctgatcagcc
     8881 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     8941 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     9001 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     9061 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     9121 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcatctagc
     9181 ggccgatcag gcccgaccta ctctcttccg cattggagtc gctttaactg gccctggctt
     9241 tggcagcctg tgctgaccca tgcagtcctc cttaccatcc ctccctcgac ttcccctctt
     9301 ccgatgttga gcccctccag ccggtcctgg actttgtctc cttccctgcc ctgccctctc
     9361 ctgaacctga gccagctccc atagctcagt ctggtctatc tgcctggccc tggccattgt
     9421 cactttgcgc tgccctcctc tcgcccccga gtgcccttgc tgtgccgccg gaactctgcc
     9481 ctctaacgct gccgtctctc tcctgagtcc ggaccacttt gagctctact ggcttctgcg
     9541 ccgcctctgg cccactgttt ccccttccca ggcaggtcct gctttctctg acctgcattc
     9601 tctcccctgg gcctgtgccg ctttctgtct gcagcttgtg gcctgggtca cctctacggc
     9661 tggcccagat ccttccctgc cgcctccttc aggttccgtc ttcctccact ccctcttccc
     9721 cttgctctct gctgtgttgc tgcccaagga tgctctttcc ggagcacttc cttctcggcg
     9781 ctgcaccacg tgatgtcctc tgagcggatc ctccccgtgt ctgggtcctc tccgggcatc
     9841 tctcctccct cacccaaccc catgccgtct tcactcgctg ggttcccttt tccttctcct
     9901 tctggggcct gtgccatctc tcgtttctta ggatggcctt ctccgacgga tgtctccctt
     9961 gcgtcccgcc tccccttctt gtaggcctgc atcaggccaa attggccgtc gactgcagag
    10021 gcctgcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
    10081 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
    10141 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
    10201 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
    10261 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    10321 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    10381 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    10441 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    10501 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    10561 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    10621 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    10681 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    10741 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    10801 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    10861 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    10921 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    10981 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    11041 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    11101 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
    11161 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
    11221 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
    11281 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
    11341 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
    11401 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
    11461 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
    11521 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
    11581 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
    11641 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
    11701 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
    11761 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    11821 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    11881 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    11941 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    12001 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    12061 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    12121 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    12181 ttccccgaaa agtgccac
//
