LOCUS       Exported               12548 bp ds-DNA     circular SYN 20-FEB-2019
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       Exported                4570 bp ds-DNA    circular SYN 
            03-FEB-2014
            DEFINITION  .
            ACCESSION   .
            VERSION     .
            KEYWORDS    SM33 - AAVS1-MCS donor
            SOURCE      synthetic DNA construct
              ORGANISM  synthetic DNA construct
            REFERENCE   1  (bases 1 to 4570)
              AUTHORS   .
              TITLE     Direct Submission
              JOURNAL   Exported Monday, Feb 03, 2014 from SnapGene 2.2.2
                        http://www.snapgene.com
            COMMENT     This file is created by Vector NTI
                        http://www.invitrogen.com/
FEATURES             Location/Qualifiers
     source          1..12548
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    625..1368
                     /label=AAVS L homology
     exon            725..1102
                     /label=exon1
     misc_feature    1255..1368
                     /label=AAVS integration site
     CDS             1379..2052
                     /label=A2UOCE
     exon            1483..1833
                     /label=CBX3 exon 1
                     /note="CBX3 exon 1"
     variation       1834
                     /label=mutant splice donor
                     /note="mutant splice donor"
     variation       1872
                     /label=mutant cryptic splice acceptor
                     /note="mutant cryptic splice acceptor"
     exon            1976..2051
                     /label=CBX3 alt exon 1
                     /note="CBX3 alt exon 1"
     promoter        2054..3232
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     intron          2285..3223
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human
                     EF-1-alpha"
     promoter        3249..3267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3366..4493
                     /codon_start=1
                     /label=HSV TK
                     /translation="MASYPCHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRLEQK
                     MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ
                     HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR
                     HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR
                     PGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGAAVPPQGAEPQSNAGPR
                     PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFILDYDQSPAGCRDAL
                     LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     CDS             4494..4559
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="GSGATNFSLLKQAGDVEENPGP"
     CDS             4560..6062
                     /codon_start=1
                     /label=Kb SCTC
                     /translation="MARSVTLVFLVLVSLTGLYAMVMSFVNLGCGASGGGGSGGGGSIQ
                     KTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWS
                     FYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDMGGGGSGGGGSGGGGSGGGGSG
                     PHSLRYFVTAVSRPGLGEPRYMEVGYVDDTEFVRFDSDAENPRYEPRARWMEQEGPEYW
                     ERETQKAKGNEQSFRVDLRTLLGCYNQSKGGSHTIQVISGCEVGSDGRLLRGYQQYAYD
                     GCDYIALNEDLKTWTAADMAALITKHKWEQAGEAERLRAYLEGTCVEWLRRYLKNGNAT
                     LLRTDSPKAHVTHHSRPEDKVTLRCWALGFYPADITLTWQLNGEELIQDMELVETRPAG
                     DGTFQKWASVVVPLGKEQYYTCHVYHQGLPEPLTLRWEPPPSTVSNMATVAVLVVLGAA
                     IVTGAVVAFVMKMRRRNTGGKGGDYALAPGSQTSDLSLPDCKA"
     CDS             6063..6125
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes ffail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="GSGEGRGSLLTCGDVEENPGP"
     CDS             6126..7100
                     /codon_start=1
                     /label=mCD47
                     /translation="MWPLAAALLLGSCCCGSAQLLFSNVNSIEFTSCNETVVIPCIVRN
                     VEAQSTEEMFVKWKLNKSYIFIYDGNKNSTTTDQNFTSAKISVSDLINGIASLKMDKRD
                     AMVGNYTCEVTELSREGKTVIELKNRTAFNTDQGSACSYEEEKGGCKLVSWFSPNEKIL
                     IVIFPILAILLFWGKFGILTLKYKSSHTNKRIILLLVAGLVLTVIVVVGAILLIPGEKP
                     VKNASGLGLIVISTGILILLQYNVFMTAFGMTSFTIAILITQVLGYVLALVGLCLCIMA
                     CEPVHGPLLISGLGIIALAELLGLVYMKFVASNQRTIQPPRNRG"
     CDS             7102..7169
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="DPASAQTMPCSSWPAMWKAIPA"
     CDS             7170..8339
                     /codon_start=1
                     /label=mCD55
                     /translation="MIRGRAPRTRPSPPPPLLPLLSLSLLLLSPTVRGDCGPPPDIPNA
                     RPILGRHSKFAEQSKVAYSCNNGFKQVPDKSNIVVCLENGQWSSHETFCEKSCVAPERL
                     SFASLKKEYLNMNFFPVGTIVEYECRPGFRKQPPLPGKATCLEDLVWSPVAQFCKKKSC
                     PNPKDLDNGHINIPTGILFGSEINFSCNPGYRLVGVSSTFCSVTGNTVDWDDEFPVCTE
                     IHCPEPPKINNGIMRGESDSYTYSQVVTYSCDKGFILVGNASIYCTVSKSDVGQWSSPP
                     PRCIEKSKVPTKKPTINVPSTGTPSTPQKPTTESVPNPGDQPTPQKPSTVKVSATQHVP
                     VTKTTVRHPIRTSTDKGEPNTGGDRYIYGHTCLITLTVLHVMLSLIGYLT"
     CDS             8340..8414
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="GSGVKQTLNFDLLKLAGDVESNPGP"
     CDS             8415..9209
                     /codon_start=1
                     /label=neomycin resistance
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    9251..9525
                     /label=BGH polyA
     misc_feature    9545..10344
                     /label=AAVS R homology
     primer_bind     complement(10399..10415)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10423..10439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10447..10477)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10492..10513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10801..11389)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    11563..12420
                     /label=Ampicillin resistance
     promoter        complement(12421..12525)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tcgcgccgtg acgtcagcac gccgggcggg gaccgggaga
      661 tccttggggc ggtggggggc cagcggcagt tcccggcggc ccccggggcg ggcgggcggg
      721 cgggtggtgg cggcggttgg ggctcggcgc tcgctcgctc gctgggcggg cgggcggtgc
      781 gatgtccgga gaggatggcc cggcggctgg cccgggggcg gcggcggcgg ctgcccggga
      841 gcggcgacgg gagcagctgc ggcagtgggg ggcgcgggcg ggcgccgagc ctggccccgg
      901 agagcgccgc gcccgcaccg tccgcttcga gcgcgccgcc gagttcctgg cggcctgtgc
      961 gggcggcgac ctggacgagg cgcgtctgat gctgcgcgcc gccgaccctg gccccggcgc
     1021 cgagctcgac cccgccgcgc cgccgcccgc ccgcgccgtg ctggactcca ccaacgccga
     1081 cggtatcagc gccctgcacc aggtcagcgc cccccgcccg gcgtctcccg gggccaggtc
     1141 caccctctgc tgcgccacct ggggcatcct ccttccccgt tgccagtctc gatccgcccc
     1201 gtcgttcctg gccctgggct ttgccaccct atgctgacac cccgtcccag tcccccttac
     1261 cattcccctt cgaccacccc acttccgaat tggagccgct tcaactggcc ctgggcttag
     1321 ccactctgtg ctgaccactc tgccccaggc ctccttacca ttccccttaa acaccggtgg
     1381 gaggtggtcc ctgctgttac gccaatgata acccccgcca gaaaaatctt agtagccttc
     1441 cctttttgtt ttccgtgccc caactcggcg gattgactcg gccccttccg gaaacacccg
     1501 aatcaacttc tagtcaaatt attgttcacg ccgcaatgac ccacccctgg cccgcgtctg
     1561 tggaactgac ccctggtgta caggagagtt cgctgctgaa agtggtccca aaggggtact
     1621 agtttttaag ctcccaactc cccctccccc agcgtctgga ggattccaca ccctcgcacc
     1681 gcaggggcga ggaagtgggc ggagtccggt tttggcgcca gccgctgagg ctgccaagca
     1741 gaaaagccac cgctgaggag actccggtca ctgtcctcgc cccgcctccc ccttccctcc
     1801 ccttggggac caccgggcgc cacgccgcga acgttaagtg ccgcggtcgt cggcgcctcc
     1861 gccctccccc ttgggcccca attcccagcg ggcgcggcgc gcggcccctc cccccgccgg
     1921 gcgcgcgccc gctgccccgc ccttcgtggc cgcccggcgt gggcggtgcc acccctcccc
     1981 ccggcggccc cgcgcgcagc tcccggctcc ctcccccttc ggatgtggct tgagctgtag
     2041 gcgcggaggg ccgggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     2101 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     2161 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     2221 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     2281 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     2341 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     2401 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     2461 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     2521 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     2581 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     2641 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     2701 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     2761 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     2821 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     2881 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     2941 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     3001 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     3061 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     3121 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     3181 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattag
     3241 cttggtacta atacgactca ctatagggag acccaagctg gctaggtaag cttggtaccg
     3301 agctcggatc cactagtcca gtgtggtgga attctgcaga tatccagcac agtggcggcc
     3361 gcaccatggc cagctatcct tgtcaccagc atgcctccgc ttttgaccag gccgctagat
     3421 ccagaggcca ttccaacagg aggacagccc tcagacccag gaggcagcaa gaggccacag
     3481 aggtcaggct ggagcagaaa atgcccaccc tcctgagggt gtacattgat ggccctcacg
     3541 gcatgggaaa gaccacaaca acccaactgc tggtcgccct gggctccagg gatgatattg
     3601 tgtacgtgcc cgaacccatg acctactgga gggtgctggg cgcctccgag accatcgcta
     3661 atatctacac cacccaacac aggctcgatc agggcgagat ttccgctggc gatgctgctg
     3721 tggtcatgac ctccgcccag atcaccatgg gcatgcccta cgccgtgaca gatgccgtcc
     3781 tggctcctca tatcggagga gaagccggca gctcccatgc tcctcctccc gccctcacac
     3841 tgatctttga cagacacccc atcgccgccc tcctgtgtta ccctgccgct agatacctga
     3901 tgggaagcat gacaccccag gccgtgctcg ctttcgtggc tctgattccc cctaccctgc
     3961 ccggcacaaa catcgtcctg ggcgctctgc ccgaagatag gcacatcgat aggctcgcca
     4021 aaaggcaaag gcccggagaa agactggacc tcgctatgct cgctgctatt aggagagtgt
     4081 acggcctcct cgccaacacc gtgagatatc tccagggcgg cggcagctgg agagaggatt
     4141 ggggccagct gtccggcgct gccgtgcctc ctcagggagc cgaacctcaa agcaatgctg
     4201 gccctagacc ccacatcggc gacaccctct tcaccctgtt tagggccccc gaactgctcg
     4261 cccccaacgg cgacctctac aacgtctttg cctgggccct ggacgtgctg gccaaaaggc
     4321 tgaggcctat gcacgtgttc atcctggact atgaccagtc ccccgccggc tgcagggatg
     4381 ccctcctcca gctgaccagc ggaatggtgc agacccacgt gacaaccccc ggcagcatcc
     4441 ccaccatttg cgacctggcc aggacattcg ccagagagat gggcgaagct aatggatccg
     4501 gagccaccaa cttcagcctg ctgaagcaag ccggcgacgt ggaggaaaac cccggcccca
     4561 tggccaggtc cgtgacactc gtcttcctgg tgctggtgtc cctgaccgga ctgtatgcta
     4621 tggtcatgag cttcgtgaat ctgggatgcg gcgccagcgg aggcggaggc tccggcggcg
     4681 gcggcagcat ccaaaagaca cctcagatcc aggtgtattc caggcaccct cctgaaaatg
     4741 gcaaacctaa tattctcaac tgttacgtca cacagtttca ccccccccac attgaaatcc
     4801 agatgctgaa gaacggcaag aaaatcccca aggtcgaaat gagcgacatg agcttcagca
     4861 aggactggtc cttttacatc ctggctcaca ccgagtttac ccctaccgag accgatacct
     4921 acgcctgcag agtgaagcac gccagcatgg ccgagcctaa gacagtctac tgggacagag
     4981 acatgggagg cggcggcagc ggcggcggcg gcagcggagg aggaggctcc ggaggaggcg
     5041 gcagcggacc ccactccctg aggtactttg tcacagccgt cagcagaccc ggactcggag
     5101 agcctaggta tatggaagtg ggatacgtgg acgacaccga gttcgtgagg tttgactccg
     5161 acgctgagaa ccctagatac gaacccaggg ccaggtggat ggagcaagag ggccctgagt
     5221 attgggaaag ggaaacccag aaggccaaag gcaacgaaca gagcttcagg gtcgacctca
     5281 gaacactgct gggctgctat aaccagagca agggaggcag ccataccatc caggtgatca
     5341 gcggctgcga ggtcggatcc gacggcagac tgctgagggg atatcagcag tacgcttacg
     5401 acggctgcga ttatattgcc ctgaacgagg atctgaaaac ctggaccgct gctgatatgg
     5461 ctgccctgat caccaagcat aagtgggaac aggctggcga ggctgagagg ctgagagctt
     5521 acctcgaagg cacatgtgtc gagtggctga ggaggtacct caagaacggc aatgccacac
     5581 tcctgagaac cgatagccct aaggctcacg tcacacatca tagcaggccc gaggacaagg
     5641 tcaccctgag atgttgggcc ctgggatttt accccgccga tatcaccctg acctggcaac
     5701 tgaacggaga ggagctgatc caggatatgg aactggtgga aaccagacct gctggcgacg
     5761 gcaccttcca aaagtgggcc agcgtggtcg tccctctggg aaaagagcaa tactatacct
     5821 gccacgtcta ccatcagggc ctgcctgagc ctctcaccct gagatgggag cctcccccca
     5881 gcaccgtcag caatatggcc accgtggctg tgctcgtggt gctgggagcc gccattgtga
     5941 caggcgccgt ggtggccttc gtcatgaaga tgaggaggag gaacaccgga ggcaaaggcg
     6001 gagactatgc cctcgctccc ggcagccaga catccgacct cagcctgccc gactgtaagg
     6061 ctggctccgg cgaaggcaga ggaagcctcc tgacctgtgg agatgtcgag gaaaatcccg
     6121 gccccatgtg gcccctggct gctgccctcc tgctgggaag ctgttgctgt ggctccgctc
     6181 agctgctgtt tagcaacgtc aactccatcg agtttacaag ctgtaacgag accgtggtca
     6241 tcccctgtat cgtgaggaac gtggaggccc agtccaccga agagatgttc gtcaagtgga
     6301 aactgaacaa aagctatatc ttcatttacg acggaaataa aaactccacc accacagacc
     6361 agaactttac cagcgccaaa atctccgtct ccgatctcat caatggcatc gccagcctga
     6421 agatggacaa gagggatgct atggtcggca attacacctg cgaggtgaca gaactcagca
     6481 gagagggcaa gaccgtgatc gagctcaaaa ataggaccgc ctttaataca gaccaaggct
     6541 ccgcttgcag ctatgaagag gaaaaaggag gatgcaagct cgtcagctgg ttttccccca
     6601 atgagaagat tctcattgtg atcttcccca tcctggccat tctcctcttt tggggcaagt
     6661 tcggaatcct caccctcaag tacaagtcca gccacaccaa caagagaatt atcctgctcc
     6721 tggtggccgg cctggtcctc accgtgatcg tggtggtcgg cgccattctg ctcattcccg
     6781 gagagaagcc tgtcaaaaac gcctccggcc tcggactcat tgtgatcagc accggaatcc
     6841 tgatcctcct gcagtacaac gtgttcatga cagctttcgg aatgacctcc ttcaccatcg
     6901 ctatcctgat tacccaggtg ctgggatatg tgctggccct cgtgggactg tgtctgtgca
     6961 tcatggcttg cgaacccgtc catggccctc tcctgatctc cggcctgggc atcatcgccc
     7021 tcgctgagct gctcggactg gtgtacatga aattcgtggc ctccaaccaa agaaccatcc
     7081 agcctcccag gaacagaggc ggatccggcc agtgcacaaa ctatgccctg ctcaagctgg
     7141 ccggcgatgt ggaaagcaat cccggcccta tgatcagggg aagggcccct aggacaaggc
     7201 ctagccctcc tccccctctc ctccctctcc tgagcctgag cctgctgctg ctgtccccta
     7261 ccgtgagagg cgactgcggc cccccccccg acattcctaa cgccagacct attctgggca
     7321 ggcatagcaa atttgccgaa caaagcaagg tcgcctacag ctgcaacaac ggcttcaagc
     7381 aggtccccga caaaagcaac attgtcgtgt gcctcgagaa cggccaatgg tccagccatg
     7441 aaacattctg cgaaaaaagc tgcgtggctc ccgagaggct gtccttcgcc tccctgaaaa
     7501 aggaatatct gaatatgaat ttcttccctg tgggcaccat tgtggagtat gagtgtaggc
     7561 ccggattcag aaagcagccc cctctgcccg gaaaagctac atgtctggaa gatctggtgt
     7621 ggagccccgt ggctcaattc tgcaaaaaga agagctgtcc caatcccaag gacctcgata
     7681 atggccacat caacattcct accggaatcc tgttcggcag cgaaattaac tttagctgca
     7741 atcccggata taggctcgtc ggcgtcagca gcaccttctg ttccgtgacc ggaaacaccg
     7801 tggactggga cgacgaattc cccgtctgta ccgagattca ttgccctgag cctcctaaga
     7861 ttaacaatgg catcatgaga ggcgaaagcg acagctacac ctattcccag gtcgtcacat
     7921 atagctgcga caagggattc atcctggtcg gcaatgctag catctattgc accgtgagca
     7981 aatccgatgt gggacaatgg agcagccccc ctcccaggtg catcgaaaag agcaaagtgc
     8041 ccaccaaaaa acccacaatc aacgtgccta gcacaggcac accttccacc cctcaaaaac
     8101 ctacaaccga gtccgtgccc aatcctggag atcagcccac ccctcagaaa ccttccacag
     8161 tcaaggtgag cgccacccag catgtccctg tgaccaagac aacagtcaga catcctatca
     8221 gaacctccac cgacaaagga gaacctaaca ccggcggaga caggtacatt tacggacaca
     8281 cctgtctgat cacactgaca gtgctccatg tcatgctgtc cctgatcgga tacctcacag
     8341 gctccggcgt gaaacaaacc ctgaacttcg atctgctgaa actcgccgga gacgtcgagt
     8401 ccaaccccgg ccctatgatc gagcaggacg gactccatgc tggaagccct gccgcttggg
     8461 tcgagaggct ctttggatac gactgggctc agcagacaat cggctgcagc gatgccgccg
     8521 tgtttagact cagcgcccag ggcagacctg tgctgtttgt gaagaccgac ctgagcggag
     8581 ctctcaatga gctccaagat gaagccgcca gactgagctg gctcgccacc acaggcgtgc
     8641 cttgtgctgc cgtgctggac gtcgtcacag aggccggaag ggattggctg ctcctgggcg
     8701 aagtccccgg acaggatctg ctgagctccc atctggcccc tgccgaaaag gtctccatta
     8761 tggccgatgc catgaggagg ctgcacaccc tggatcccgc tacctgtccc tttgaccatc
     8821 aggccaagca caggattgag agggccagaa caagaatgga ggccggcctg gtcgaccagg
     8881 atgatctgga cgaggaacac cagggcctcg ctcctgccga gctctttgcc agactcaagg
     8941 ccaggatgcc tgacggcgaa gatctggtgg tgacccatgg agatgcctgt ctccccaaca
     9001 tcatggtgga aaacggcagg ttctccggat tcattgactg cggcaggctc ggcgtggccg
     9061 acaggtatca ggacatcgcc ctggctacca gagatatcgc tgaggagctg ggaggagagt
     9121 gggccgatag atttctcgtg ctctatggca ttgccgctcc cgacagccag agaattgcct
     9181 tctacagact gctcgatgag tttttttgag tttaaacccg ctgatcagcc tcgactgtgc
     9241 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     9301 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     9361 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     9421 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     9481 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcatctagc ggccgatcag
     9541 gcccgaccta ctctcttccg cattggagtc gctttaactg gccctggctt tggcagcctg
     9601 tgctgaccca tgcagtcctc cttaccatcc ctccctcgac ttcccctctt ccgatgttga
     9661 gcccctccag ccggtcctgg actttgtctc cttccctgcc ctgccctctc ctgaacctga
     9721 gccagctccc atagctcagt ctggtctatc tgcctggccc tggccattgt cactttgcgc
     9781 tgccctcctc tcgcccccga gtgcccttgc tgtgccgccg gaactctgcc ctctaacgct
     9841 gccgtctctc tcctgagtcc ggaccacttt gagctctact ggcttctgcg ccgcctctgg
     9901 cccactgttt ccccttccca ggcaggtcct gctttctctg acctgcattc tctcccctgg
     9961 gcctgtgccg ctttctgtct gcagcttgtg gcctgggtca cctctacggc tggcccagat
    10021 ccttccctgc cgcctccttc aggttccgtc ttcctccact ccctcttccc cttgctctct
    10081 gctgtgttgc tgcccaagga tgctctttcc ggagcacttc cttctcggcg ctgcaccacg
    10141 tgatgtcctc tgagcggatc ctccccgtgt ctgggtcctc tccgggcatc tctcctccct
    10201 cacccaaccc catgccgtct tcactcgctg ggttcccttt tccttctcct tctggggcct
    10261 gtgccatctc tcgtttctta ggatggcctt ctccgacgga tgtctccctt gcgtcccgcc
    10321 tccccttctt gtaggcctgc atcaggccaa attggccgtc gactgcagag gcctgcatgc
    10381 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
    10441 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
    10501 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
    10561 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    10621 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10681 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    10741 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    10801 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    10861 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    10921 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    10981 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11041 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
    11101 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
    11161 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
    11221 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
    11281 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
    11341 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
    11401 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
    11461 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
    11521 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
    11581 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
    11641 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
    11701 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
    11761 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
    11821 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
    11881 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
    11941 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
    12001 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
    12061 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
    12121 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
    12181 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
    12241 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
    12301 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
    12361 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
    12421 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
    12481 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
    12541 agtgccac
//
