LOCUS       Exported               11251 bp ds-DNA     circular SYN 05-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11251)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 6, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pFG12                  12625 bp    DNA     circular     
            17-JUN-2008

            SOURCE      

              ORGANISM  

            COMMENT     This file is created by Vector NTI

                        http://www.invitrogen.com/

            COMMENT     ORIGDB|GenBank

            COMMENT     VNTDATE|485364355|

            COMMENT     VNTDBDATE|485364355|

            COMMENT     LSOWNER|

            COMMENT     VNTNAME|pFG12|

            COMMENT     VNTAUTHORNAME|Demo User|
FEATURES             Location/Qualifiers
     source          1..11251
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..1015
                     /label=CMV-5'LTR
     misc_signal     1574..2431
                     /label=RRE
     misc_signal     2444..2459
                     /label=cPPT
     promoter        2600..2920
                     /label=Tet responsive promoter
     misc_feature    2921
                     /label=potential EcoR I site
                     /note="potential EcoR I site"
     primer_bind     complement(2977..2995)
                     /label=TRE rev sequencing
     promoter        2983..4199
                     /label=UBC promoter
     CDS             4236..5105
                     /codon_start=1
                     /label=PDL1
                     /translation="MRIFAGIIFTACCHLLRAFTITAPKDLYVVEYGSNVTMECRFPVE
                     RELDLLALVVYWEKEDEQVIQFVAGEEDLKPQHSNFRGRASLPKDQLLKGNAALQITDV
                     KLQDAGVYCCIISYGGADYKRITLKVNAPYRKINQRISVDPATSEHELICQAEGYPEAE
                     VIWTNSDHQPVSGKRSVTTSRTEGMLLNVTSSLRVNATANDVFYCTFWRSQPGQNHTAE
                     LIIPELPATHPPQNRTHWVLLGSILLFLIVVSTVLLFLRKQVRMLDVEKCGVEDTSSKN
                     RNDTQFEET"
     CDS             5106..5159
                     /codon_start=1
                     /label=T2A
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5160..5879
                     /codon_start=1
                     /label=emGFP
                     /note="emGFP"
                     /translation="VVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_signal     5935..6529
                     /label=WPRE
     LTR             6538..7246
                     /label=trunc 3'LTR
     gene            10258..11118
                     /label=Ampicillin resistance gene
                     /note="Ampicillin resistance gene"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagttttact ccctatcagt gatagagaac gtatgtcgag
     2641 tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag tgatagagaa
     2701 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc
     2761 agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt atgtcgagtt
     2821 tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat
     2881 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattcgtta acggatcctc
     2941 tagagctcag cctcgagatt taaatttaat taacccaccc aagatctggc ctccgcgccg
     3001 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     3061 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     3121 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     3181 tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa agtagtccct
     3241 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     3301 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     3361 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     3421 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     3481 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     3541 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     3601 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     3661 aagctcttat tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     3721 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     3781 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     3841 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     3901 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     3961 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     4021 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     4081 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     4141 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     4201 aagcttggta ccgagctcgg atctccaccc cgtacatgag gatatttgct ggcattatat
     4261 tcacagcctg ctgtcacttg ctacgggcgt ttactatcac ggctccaaag gacttgtacg
     4321 tggtggagta tggcagcaac gtcacgatgg agtgcagatt ccctgtagaa cgggagctgg
     4381 acctgcttgc gttagtggtg tactgggaaa aggaagatga gcaagtgatt cagtttgtgg
     4441 caggagagga ggaccttaag cctcagcaca gcaacttcag ggggagagcc tcgctgccaa
     4501 aggaccagct tttgaaggga aatgctgccc ttcagatcac agacgtcaag ctgcaggacg
     4561 caggcgttta ctgctgcata atcagctacg gtggtgcgga ctacaagcga atcacgctga
     4621 aagtcaatgc cccataccgc aaaatcaacc agagaatttc cgtggatcca gccacttctg
     4681 agcatgaact aatatgtcag gccgagggtt atccagaagc tgaggtaatc tggacaaaca
     4741 gtgaccacca acccgtgagt gggaagagaa gtgtcaccac ttcccggaca gaggggatgc
     4801 ttctcaatgt gaccagcagt ctgagggtca acgccacagc gaatgatgtt ttctactgta
     4861 cgttttggag atcacagcca gggcaaaacc acacagcgga gctgatcatc ccagaactgc
     4921 ctgcaacaca tcctccacag aacaggactc actgggtgct tctgggatcc atcctgttgt
     4981 tcctcattgt agtgtccacg gtcctcctct tcttgagaaa acaagtgaga atgctagatg
     5041 tggagaaatg tggcgttgaa gatacaagct caaaaaaccg aaatgataca caattcgagg
     5101 agacggaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat cccggccctg
     5161 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     5221 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     5281 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     5341 tcgtgaccac cttgacctac ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc
     5401 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     5461 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     5521 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     5581 agctggagta caactacaac agccacaagg tctatatcac cgccgacaag cagaagaacg
     5641 gcatcaaggt gaacttcaag acccgccaca acatcgagga cggcagcgtg cagctcgccg
     5701 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     5761 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     5821 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat
     5881 gtacaagtaa agcggccgcg actctagtcc ctcccaattc gatatcaagc ttatcgataa
     5941 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     6001 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     6061 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     6121 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6181 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6241 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6301 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     6361 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6421 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6481 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     6541 acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     6601 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     6661 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     6721 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     6781 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     6841 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     6901 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     6961 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     7021 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     7081 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7141 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7201 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     7261 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     7321 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     7381 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     7441 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     7501 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     7561 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     7621 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     7681 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     7741 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     7801 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     7861 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     7921 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     7981 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8041 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     8101 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8161 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8221 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     8281 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     8341 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     8401 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     8461 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     8521 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     8581 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     8641 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     8701 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     8761 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     8821 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     8881 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     8941 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     9001 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     9061 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     9121 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     9181 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     9241 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9301 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9361 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9421 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9481 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9541 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9601 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9661 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9721 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9781 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9841 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9901 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9961 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    10021 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    10081 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    10141 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    10201 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    10261 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10321 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10381 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10441 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10501 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10561 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10621 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10681 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10741 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10801 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10861 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10921 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10981 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    11041 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    11101 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    11161 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    11221 gcgcacattt ccccgaaaag tgccacctga c
//
