LOCUS       Vector_795J1=pBa        8754 bp DNA     circular SYN 01-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 6, 2023 from SnapGene 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..616
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          617..994
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          617..994
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          995..1002
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          5965..6250
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          6566..8754
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     305..364
                     /label=1110D.c3F
                     /note="color: black; sequence: 
                     cgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagc
                     g"
     primer_bind     complement(587..646)
                     /label=1110H.c2R
                     /note="color: black; sequence: 
                     ACAATATGATTATCTTTCTAGGGTTAATCTactggccgtcgttttacaacgtcgtgact
                     g"
     primer_bind     592..646
                     /label=1110H.c7F
                     /note="color: black; sequence: 
                     acgacgttgtaaaacgacggccagtAGATTAACCCTAGAAAGATAATCATATTGT"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     618..683
                     /label=1110H.c6R
                     /note="color: black; sequence: 
                     cgaaattaaccctcactaaaggGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG
                     C"
     misc_feature    620..623
                     /label=TTAA
                     /note="color: #ffffff"
     misc_feature    complement(624..824)
                     /label=3' pBac transposon
                     /label=nonstandard type: transposon
                     /note="3' pBac"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(819..867)
                     /label=1110H.c8R
                     /note="color: black; sequence: 
                     cttattatcatcttgtgatgaggatgcttctatcaacgaaagtTTTGTT"
     misc_feature    825..994
                     /label=Part of Omar's piggyBacR
                     /note="color: #99ccff"
     primer_bind     889..916
                     /label=803A
                     /note="color: black; sequence: 
                     GGTTTATATGAGACGAGAGTAAGGGGTC"
     regulatory      1003..1389
                     /label=gypsy insulator
                     /note="gypsy"
                     /note="color: #ffffff"
                     /regulatory_class="insulator"
     primer_bind     complement(1003..1027)
                     /label=Primer 4
                     /note="color: black; sequence: 
                     aattcaacgcacacttattacgtgaggtac"
     primer_bind     1360..1389
                     /label=1110F.c1F
                     /note="color: black; sequence: 
                     tttcttgccataccatttagccgatcaattCCGCGGcatactcggtggcctccccacca
                     c"
     primer_bind     1363..1422
                     /label=1110I.c4R
                     /note="color: black; sequence: 
                     cttgccataccatttagccgatcaattgcggccgcTCGAATCGCTATCCAAGCCAGCTC
                     C"
     primer_bind     1366..1389
                     /label=1122I1.c1F
                     /note="color: black; sequence: 
                     gccataccatttagccgatcaattCTCGAGcgcgtaaaacacaatcaagtatgagtcat
                     a"
     3'UTR           complement(1398..2057)
                     /label=p10 3'UTR
                     /note="color: #ffff00; direction: LEFT"
     primer_bind     2034..2093
                     /label=1122J.c1F
                     /note="color: black; sequence: 
                     TTTGTTATTTTAAAAACGATTCATCCTAGGctacaggaacaggtggtggcggccctcgg
                     t"
     primer_bind     2037..2057
                     /label=1110I.c2R
                     /note="color: black; sequence: 
                     GTTATTTTAAAAACGATTCATggatccCTATTACTTTTTCTTTTTTGCCTGGCCGGCCT
                     T"
     misc_feature    2064..2741
                     /label=Dsred
                     /note="Dsred"
                     /note="color: #ff0000"
     misc_feature    2064..2097
                     /label=misc_feature 2
                     /note="color: #ff8000"
     primer_bind     2064..2093
                     /label=Primer 1
                     /note="color: black; sequence: 
                     ctacaggaacaggtggtggcggccctcggt"
     misc_feature    2064..2085
                     /label=misc_feature 5
                     /note="color: #50ff50; direction: RIGHT"
     misc_feature    2064..2085
                     /label=misc_feature 6
                     /note="color: #50ff50"
     CDS             complement(2067..2741)
                     /label=DsRed (RFP)
                     /note="color: #0000ff; direction: LEFT"
     primer_bind     complement(2405..2430)
                     /label=1122M.c4R
                     /note="color: black; sequence: 
                     tcttcttccacttccacgtagGTGACCGTGACCCAGGACTCCTCCC"
     misc_feature    2409..2417
                     /label=pt mutation
                     /note="pt mutation"
                     /note="color: #ff8000"
     primer_bind     2428..2454
                     /label=1122M.c1F
                     /note="color: black; sequence: 
                     gatcgaatatgtgtatatatacCACGCCGCCGTCCTCGAAGTTCATC"
     misc_feature    2436..2455
                     /label=misc_feature 7
                     /note="color: #50ff50"
     misc_feature    2436..2455
                     /label=misc_feature 8
                     /note="color: #50ff50; direction: RIGHT"
     primer_bind     2585..2594
                     /label=1110I.c2R
                     /note="color: black; sequence: 
                     GTTATTTTAAAAACGATTCATggatccCTATTACTTTTTCTTTTTTGCCTGGCCGGCCT
                     T"
     misc_feature    2711..2741
                     /label=misc_feature 21
                     /note="color: #50ff50; direction: RIGHT"
     primer_bind     complement(2712..2764)
                     /label=1122M1.c3R
                     /note="color: black; sequence: 
                     cctcttcttccacttccacgtaggtgcgctcctccaagaacgtcatcaaggag"
     primer_bind     2712..2764
                     /label=1122M1.c1F
                     /note="color: black; sequence: 
                     ctccttgatgacgttcttggaggagcgcacctacgtggaagtggaagaagagg"
     primer_bind     complement(2715..2742)
                     /label=1122J.c2R
                     /note="color: black; sequence: 
                     AGATCGTGAACAACCAAGTGACttaattaaatggtgcgctcctccaagaacgtcatcaa
                     g"
     primer_bind     complement(2715..2742)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     atggtgcgctcctccaagaacgtcatcaag"
     primer_bind     complement(2715..2742)
                     /label=795G2.c1R
                     /note="color: black; sequence: 
                     ccgcaacctgtctctggtgGGATCCatggtgcgctcctccaagaacgtcatcaag; 
                     added: 2022-07-19"
     primer_bind     complement(2715..2742)
                     /label=Primer 2a
                     /note="color: black; sequence: 
                     atggtgcgctcctccaagaacgtcatcaag"
     primer_bind     complement(2715..2742)
                     /label=Primer 2a
                     /note="color: black; sequence: 
                     atggtgcgctcctccaagaacgtcatcaag"
     primer_bind     complement(2715..2742)
                     /label=Primer 2a
                     /note="color: black; sequence: 
                     atggtgcgctcctccaagaacgtcatcaag"
     primer_bind     2723..2742
                     /label=1122J1.c1F
                     /note="color: black; sequence: 
                     cgttcttggaggagcgcaccatttaattaacaccagagacaggttgcgg"
     primer_bind     2723..2742
                     /label=Primer 3
                     /note="color: black; sequence: 
                     cgttcttggaggagcgcaccatttaattaa"
     misc_feature    2724..2741
                     /label=misc_feature 9
                     /note="color: #ff8000"
     misc_feature    2724..2741
                     /label=misc_feature 10
                     /note="color: #50ff50; direction: RIGHT"
     primer_bind     complement(2739..2762)
                     /label=1122M.c4R
                     /note="color: black; sequence: 
                     tcttcttccacttccacgtagGTGACCGTGACCCAGGACTCCTCCC"
     misc_feature    2742..2972
                     /label=Ds TraF intron
                     /note="color: #a6acb3"
     primer_bind     2742..2764
                     /label=Primer 3a
                     /note="color: black; sequence: ctacgtggaagtggaagaagagg"
     primer_bind     complement(2943..2994)
                     /label=1122M1.c2R
                     /note="color: black; sequence: 
                     ccgcaacctgtctctggtgttaattaaatggtatatatacacatattcgatcggatcag
                     a"
     primer_bind     complement(2943..2994)
                     /label=795P.c1F
                     /note="color: black; sequence: 
                     ccgcaacctgtctctggtgatggtatatatacacatattcgatcggatcaga; 
                     added: 2022-05-20"
     primer_bind     complement(2943..2972)
                     /label=Primer 4a
                     /note="color: black; sequence: 
                     gtatatatacacatattcgatcggatcaga"
     primer_bind     2951..2974
                     /label=1122M.c1F
                     /note="color: black; sequence: 
                     gatcgaatatgtgtatatatacCACGCCGCCGTCCTCGAAGTTCATC"
     primer_bind     complement(2970..3007)
                     /label=795I
                     /note="color: black; sequence: 
                     CATCCAACCGCCGCCGCAACCTGTCTCTGGTGATGGTACCCACTATATAGTATAACCGA
                     TCTGAT; added: 2022-08-01"
     misc_feature    2973..2975
                     /label=misc_feature 19
                     /note="color: #50ff50"
     misc_feature    complement(2976..3522)
                     /label=Opie2 promoter
                     /note="Opie2 promoter"
                     /note="color: #0080ff; direction: LEFT"
     primer_bind     2976..2994
                     /label=1122J1.c1F
                     /note="color: black; sequence: 
                     cgttcttggaggagcgcaccatttaattaacaccagagacaggttgcgg"
     primer_bind     2976..2994
                     /label=CACCAGAGACAGGTTGCGG
                     /note="color: black; sequence: CACCAGAGACAGGTTGCGG"
     primer_bind     complement(2976..2994)
                     /label=795G2.c1R
                     /note="color: black; sequence: 
                     ccgcaacctgtctctggtgGGATCCatggtgcgctcctccaagaacgtcatcaag; 
                     added: 2022-07-19"
     primer_bind     complement(3306..3315)
                     /label=1110I.c2R
                     /note="color: black; sequence: 
                     GTTATTTTAAAAACGATTCATggatccCTATTACTTTTTCTTTTTTGCCTGGCCGGCCT
                     T"
     primer_bind     3448..3477
                     /label=1122G.c2R
                     /note="color: black; sequence: 
                     acctacatcgtcgacgggcccgcgtgcaaaagcgtgtttttttgc"
     primer_bind     complement(3448..3462)
                     /label=1122G.c1F
                     /note="color: black; sequence: 
                     tttgcacgcgggcccgtcgacgatgtaggtcacggtctcgaagcc"
     primer_bind     complement(3448..3462)
                     /label=F1
                     /note="color: black; sequence: tttgcacgcgggccc"
     primer_bind     complement(3493..3516)
                     /label=1110F.c1F
                     /note="color: black; sequence: 
                     tttcttgccataccatttagccgatcaattCCGCGGcatactcggtggcctccccacca
                     c"
     primer_bind     complement(3496..3516)
                     /label=CATACTCGGTGGCCTCCCCAC
                     /note="color: black; sequence: CATACTCGGTGGCCTCCCCAC"
     primer_bind     complement(3523..3552)
                     /label=Primer 4
                     /note="color: black; sequence: 
                     aattcaacgcacacttattacgtgaggtac"
     regulatory      3528..3914
                     /label=gypsy insulator
                     /note="gypsy"
                     /note="color: #ffffff"
                     /regulatory_class="insulator"
     primer_bind     3885..3915
                     /label=1110F.c1F
                     /note="color: black; sequence: 
                     tttcttgccataccatttagccgatcaattCCGCGGcatactcggtggcctccccacca
                     c"
     primer_bind     3888..3914
                     /label=1110I.c4R
                     /note="color: black; sequence: 
                     cttgccataccatttagccgatcaattgcggccgcTCGAATCGCTATCCAAGCCAGCTC
                     C"
     primer_bind     3891..3950
                     /label=1122I1.c1F
                     /note="color: black; sequence: 
                     gccataccatttagccgatcaattCTCGAGcgcgtaaaacacaatcaagtatgagtcat
                     a"
     misc_feature    3921..5002
                     /label=Hr5Ie1
                     /note="Hr5Ie1"
                     /note="color: #ff8000; direction: RIGHT"
     enhancer        3921..4403
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
                     /note="color: #a6acb3"
     promoter        4407..4998
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     4488..4516
                     /label=1140A.C1F
                     /note="color: black; sequence: 
                     ccttggcgtagaatttgtcgggtccattg"
     primer_bind     complement(4974..5033)
                     /label=1140A.C2R
                     /note="color: black; sequence: 
                     AACAGCTCCTCGCCCTTGCTCATtttgTATTgtcacttggttgttcacgatcttgtcgc
                     c"
     primer_bind     4981..5002
                     /label=1122J.c2R
                     /note="color: black; sequence: 
                     AGATCGTGAACAACCAAGTGACttaattaaatggtgcgctcctccaagaacgtcatcaa
                     g"
     primer_bind     4983..5036
                     /label=1140A.C3F
                     /note="color: black; sequence: 
                     atcgtgaacaaccaagtgacAATAcaaaATGAGCAAGGGCGAGGAGCTGTTCAC"
     misc_feature    5007..5010
                     /label=Kozak-seq
                     /note="color: #a6acb3; direction: RIGHT"
     CDS             5011..5727
                     /codon_start=1
                     /label=eGFP
                     /note="color: #00ff00"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(5274..5299)
                     /label=1068D.s1R
                     /note="color: black; sequence: TGCGCTCCTGGACGTAGCCTTCGGGC"
     primer_bind     complement(5698..5749)
                     /label=1102D.C2R
                     /note="color: black; sequence: 
                     ggtatggctgattatgatctagTTACTTGTACAGCTCGTCCATGCCGAGAGT"
     primer_bind     5715..5757
                     /label=1102D.C3F
                     /note="color: black; sequence: 
                     GCTGTACAAGTAActagatcataatcagccataccacatttgt"
     misc_feature    5728..5961
                     /label=Sv40
                     /note="Sv40"
                     /note="color: #99ccff"
     polyA_signal    5837..5958
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     5930..5989
                     /label=1110H.c3F
                     /note="color: black; sequence: 
                     ggtttgtccaaactcatcaatgtatcttaaagcttgtcgacgatgtaggtcacggtctc
                     g"
     primer_bind     complement(5935..6024)
                     /label=1122J1.c2R
                     /note="color: black; sequence: 
                     cccaagggcacgccctggcacccgcaccgcggcttcgagaccgtgacctacatcgtcga
                     caagctttaagatacattgatgagtttggac"
     primer_bind     complement(5937..5962)
                     /label=1140A.C4R
                     /note="color: black; sequence: 
                     tgaacattgtcagatccgagatcGGCCGGCCTAGgctttaagatacattgatgagtttg
                     g"
     misc_feature    5965..6250
                     /label=short-attP site
                     /note="color: #ff99cc"
     primer_bind     5965..5994
                     /label=1122G.c1F
                     /note="color: black; sequence: 
                     tttgcacgcgggcccgtcgacgatgtaggtcacggtctcgaagcc"
     primer_bind     complement(5965..5979)
                     /label=F2
                     /note="color: black; sequence: acctacatcgtcgac"
     primer_bind     complement(5965..5979)
                     /label=1122G.c2R
                     /note="color: black; sequence: 
                     acctacatcgtcgacgggcccgcgtgcaaaagcgtgtttttttgc"
     primer_bind     complement(5997..6026)
                     /label=Primer 5
                     /note="color: black; sequence: 
                     agcccaagggcacgccctggcacccgcacc"
     primer_bind     6219..6249
                     /label=1110H.c5F
                     /note="color: black; sequence: 
                     ggttctcgacggtcacggcgggcatgtcgac"
     primer_bind     complement(6225..6269)
                     /label=1110H.c4R
                     /note="color: black; sequence: 
                     TTTTCTTGTTATAGATATCtgtcgacatgcccgccgtgaccgtcg"
     misc_feature    complement(6251..6559)
                     /label=5' pBac transposon
                     /label=nonstandard type: transposon
                     /note="5' pBac"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(6419..6439)
                     /label=803C
                     /note="color: black; sequence: CCGTGAGGCGTGCTTGTCAAT"
     primer_bind     complement(6528..6587)
                     /label=1110H.c6R
                     /note="color: black; sequence: 
                     cgaaattaaccctcactaaaggGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG
                     C"
     primer_bind     6536..6594
                     /label=1110H.c1F
                     /note="color: black; sequence: 
                     TTTACGCAGACTATCTTTCTAGGGTTAATCcctttagtgagggttaatttcgagcttgg
                     "
     misc_feature    6560..6563
                     /label=TTAA
                     /note="color: #a6acb3"
     promoter        complement(6566..6584)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(6605..6621)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6629..6645
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6653..6683)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6653 .. 6659 / #ffffff / -10
                        2: 6660 .. 6677 / #ffffff
                        3: 6678 .. 6683 / #ffffff / -35"
     protein_bind    6698..6719
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(6701..6726)
                     /label=803F
                     /note="color: black; sequence: acgcaattaatgtgagttagctcact"
     rep_origin      complement(7007..7595)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7766..8626)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7766 .. 8557 / #ccffcc
                        2: 8558 .. 8626 / #ccffcc / signal sequence
                     Cleavage site after base 8557"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8627..8731)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtagat taaccctaga aagataatca tattgtgacg tacgttaaag
      661 ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
      721 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
      781 caaacaattt atttatgttt atttatttat taaaaaaaaa caaaactttc gttgatagaa
      841 gcatcctcat cacaagatga taataagtat accatcttag ctggcttcgg tttatatgag
      901 acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc ccactggcct ggagcgactg
      961 tttttcagta cttccggtat ctcgcgtttg tttgcctgca ggtcacgtaa taagtgtgcg
     1021 ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag taaaattttg ttgcatacct
     1081 tatcaaaaaa taagtgctgc atacttttta gagaaaccaa ataatttttt attgcatacc
     1141 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tactttgacg
     1201 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcataccc gtttttaata
     1261 aaatacattg cataccctct tttaataaaa aatattgcat acgttgacga aacaaatttt
     1321 cgttgcatac ccaataaaag attattatat tgcatacctt ttcttgccat accatttagc
     1381 cgatcaattg cggccgctcg aatcgctatc caagccagct ccattgtcgg catcgtgctc
     1441 attctattga cgctggcaga tttggttttg gcgctatggg acccgttcgg ttacaacaac
     1501 atgtttccgc gcgagtttcc cgacgacatg tcgcgcacgt tcctgactgc gtactttgag
     1561 agtttcgaca acaccacgtc cagagaaatc atagagttta tgcccgagtt cttttcggaa
     1621 atggtcgaaa cggacgatga cgccacgttt gaatctctat ttcatttatt agattatgtg
     1681 gcatctttag aagttaattc cgacggccaa atgttaaact tggaggaggg tgatgaaatt
     1741 gaggattttg acgaatctac tttggtgggg caagcgttag ccactagctc gctatacact
     1801 cgcatggagt ttatgcagta cacgtttagg caaaacacac tattgtctat gaacaaagaa
     1861 aacaacaatt ttaatcaaat aatcatgggt ttatttgcaa caaacacaat tgtggcgttt
     1921 acagcatttg ttatacacac agaactcata ttttttatat ttttcgtaat cttcctaatg
     1981 atcacatttt attacataat caaagaatcg tacgaatatt ataaaacaat tgatttgtta
     2041 ttttaaaaac gattcatcct aggctacagg aacaggtggt ggcggccctc ggtgcgctcg
     2101 tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg
     2161 tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc
     2221 aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg
     2281 tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
     2341 gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc
     2401 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
     2461 tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc
     2521 ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc
     2581 agggggccgc ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc
     2641 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg
     2701 aagcgcatga actccttgat gacgttcttg gaggagcgca cctacgtgga agtggaagaa
     2761 gaggtgttaa tcactccagc cgtagtaagt ggtgcgaatt ttggggtcca cataatgcgc
     2821 agttgagtgt ccagttgtcc tagcttcttt gtctgctcgt ccagcgttca gcttcatgcc
     2881 attcacattt cacacaattt ggcactagaa aaaaaacaac aaaaagtttg cacttgatca
     2941 attctgatcc gatcgaatat gtgtatatat accatcacca gagacaggtt gcggcggcgg
     3001 ttggatggcg tgggcgcgtt ggcgttgttg gaccggctca tgttgtgtcg ctgtaacaga
     3061 tgctgttcaa ctgtgtttac cagatcgttg cgggctgtat ttataggcgc gataagcggg
     3121 acgggcgcct cgtgtccggt cacgcgcatg agataacgcg cggctgatat ggaggcgcgt
     3181 cctgttccga taaggagttg cgtccggctg cggttagcaa cacaggaagc tggcgtcctg
     3241 tcacgataag acaacactcg tccggtccga taatgtgatt cgtacgtgac aggacgcgac
     3301 ccgataaggc cggcctacgt gactgccgac acgtactttt ttgcactgca aaaaggttca
     3361 atgtgtggta gtgtatttgg agcgtataca acggtgtaga ctatttatgt aaaatagtct
     3421 acgaaacgta gagtttgtac tatgtatggg cccgcgtgca aaagcgtgtt tttttgcagt
     3481 gcaaaaaagt tggtggtggg gaggccaccg agtatgggcg cggtacctca cgtaataagt
     3541 gtgcgttgaa tttattcgca aaaacattgc atattttcgg caaagtaaaa ttttgttgca
     3601 taccttatca aaaaataagt gctgcatact ttttagagaa accaaataat tttttattgc
     3661 atacccgttt ttaataaaat acattgcata ccctctttta ataaaaaata ttgcatactt
     3721 tgacgaaaca aattttcgtt gcatacccaa taaaagatta ttatattgca tacccgtttt
     3781 taataaaata cattgcatac cctcttttaa taaaaaatat tgcatacgtt gacgaaacaa
     3841 attttcgttg catacccaat aaaagattat tatattgcat accttttctt gccataccat
     3901 ttagccgatc aattctcgag cgcgtaaaac acaatcaagt atgagtcata agctgatgtc
     3961 atgttttgca cacggctcat aaccgaactg gctttacgag tagaattcta cttgtaacgc
     4021 acgatcagtg gatgatgtca tttgtttttc aaatcgagat gatgtcatgt tttgcacacg
     4081 gctcataaac tcgctttacg agtagaattc tacgtgtaac gcacgatcga ttgatgagtc
     4141 atttgttttg caatatgata tcatacaata tgactcattt gtttttcaaa accgaacttg
     4201 atttacgggt agaattctac ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc
     4261 aaaactgaac tcgctttacg agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt
     4321 catttgttat aaaaataaaa gctgatgtca tgttttgcac atggctcata actaaactcg
     4381 ctttacgggt agaattctac gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag
     4441 gttattgata aaatgaacgg atacgttgcc cgacattatc attaaatcct tggcgtagaa
     4501 tttgtcgggt ccattgtccg tgtgcgctag catgcccgta acggacctcg tacttttggc
     4561 ttcaaaggtt ttgcgcacag acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt
     4621 taccacaaat cccaacggcg cagtgtactt gttgtatgca aataaatctc gataaaggcg
     4681 cggcgcgcga atgcagctga tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat
     4741 cgacctcaga ttaatgttta tcggccgact gttttcgtat ccgctcacca aacgcgtttt
     4801 tgcattaaca ttgtatgtcg gcggatgttc tatatctaat ttgaataaat aaacgataac
     4861 cgcgttggtt ttagagggca taataaaaga aatattgtta tcgtgttcgc cattagggca
     4921 gtataaattg acgttcatgt tggatattgt ttcagttgca agttgacact ggcggcgaca
     4981 agatcgtgaa caaccaagtg acaatacaaa atgagcaagg gcgaggagct gttcaccggg
     5041 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     5101 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     5161 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     5221 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     5281 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     5341 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     5401 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     5461 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     5521 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     5581 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     5641 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     5701 ctcggcatgg acgagctgta caagtaacta gatcataatc agccatacca catttgtaga
     5761 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     5821 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     5881 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     5941 actcatcaat gtatcttaaa gcttgtcgac gatgtaggtc acggtctcga agccgcggtg
     6001 cgggtgccag ggcgtgccct tgggctcccc gggcgcgtac tccacctcac ccatctggtc
     6061 catcatgatg aacgggtcga ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg
     6121 gaagccctcg ccctcgaaac cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac
     6181 ggcgtcggcg ggtgcggata cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg
     6241 catgtcgaca gatatctata acaagaaaat atatatataa taagttatca cgtaagtaga
     6301 acatgaaata acaatataat tatcgtatga gttaaatctt aaaagtcacg taaaagataa
     6361 tcatgcgtca ttttgactca cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat
     6421 tgacaagcac gcctcacggg agctccaagc ggcgactgag atgtcctaaa tgcacagcga
     6481 cggattcgcg ctatttagaa agagagagca atatttcaag aatgcatgcg tcaattttac
     6541 gcagactatc tttctagggt taatcccttt agtgagggtt aatttcgagc ttggcgtaat
     6601 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     6661 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     6721 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     6781 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     6841 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     6901 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6961 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     7021 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     7081 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     7141 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     7201 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     7261 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     7321 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     7381 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     7441 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     7501 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     7561 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     7621 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     7681 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     7741 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     7801 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     7861 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     7921 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7981 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     8041 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     8101 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     8161 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     8221 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     8281 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     8341 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     8401 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     8461 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     8521 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     8581 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     8641 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     8701 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccac
//
