LOCUS       Vector_795N=pBac        8760 bp DNA     circular SYN 24-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8760)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 6, 2023 from SnapGene 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8760
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..616
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          617..994
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          617..994
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          995..1002
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          5971..6256
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          6572..8760
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     305..364
                     /label=1110D.c3F
                     /note="color: black; sequence: 
                     cgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagc
                     g"
     primer_bind     complement(587..646)
                     /label=1110H.c2R
                     /note="color: black; sequence: 
                     ACAATATGATTATCTTTCTAGGGTTAATCTactggccgtcgttttacaacgtcgtgact
                     g"
     primer_bind     592..646
                     /label=1110H.c7F
                     /note="color: black; sequence: 
                     acgacgttgtaaaacgacggccagtAGATTAACCCTAGAAAGATAATCATATTGT"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     618..683
                     /label=1110H.c6R
                     /note="color: black; sequence: 
                     cgaaattaaccctcactaaaggGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG
                     C"
     misc_feature    620..623
                     /label=TTAA
                     /note="color: #ffffff"
     misc_feature    complement(624..824)
                     /label=3' pBac transposon
                     /label=nonstandard type: transposon
                     /note="3' pBac"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(819..867)
                     /label=1110H.c8R
                     /note="color: black; sequence: 
                     cttattatcatcttgtgatgaggatgcttctatcaacgaaagtTTTGTT"
     misc_feature    825..994
                     /label=Part of Omar's piggyBacR
                     /note="color: #99ccff"
     primer_bind     889..916
                     /label=803A
                     /note="color: black; sequence: 
                     GGTTTATATGAGACGAGAGTAAGGGGTC"
     regulatory      1003..1389
                     /label=gypsy insulator
                     /note="gypsy"
                     /note="color: #ffffff"
                     /regulatory_class="insulator"
     primer_bind     complement(1003..1027)
                     /label=Primer 4
                     /note="color: black; sequence: 
                     aattcaacgcacacttattacgtgaggtac"
     primer_bind     1360..1389
                     /label=1110F.c1F
                     /note="color: black; sequence: 
                     tttcttgccataccatttagccgatcaattCCGCGGcatactcggtggcctccccacca
                     c"
     primer_bind     1363..1422
                     /label=1110I.c4R
                     /note="color: black; sequence: 
                     cttgccataccatttagccgatcaattgcggccgcTCGAATCGCTATCCAAGCCAGCTC
                     C"
     primer_bind     1366..1389
                     /label=1122I1.c1F
                     /note="color: black; sequence: 
                     gccataccatttagccgatcaattCTCGAGcgcgtaaaacacaatcaagtatgagtcat
                     a"
     3'UTR           complement(1398..2057)
                     /label=p10 3'UTR
                     /note="color: #ffff00; direction: LEFT"
     primer_bind     2034..2093
                     /label=1122J.c1F
                     /note="color: black; sequence: 
                     TTTGTTATTTTAAAAACGATTCATCCTAGGctacaggaacaggtggtggcggccctcgg
                     t"
     primer_bind     2037..2057
                     /label=1110I.c2R
                     /note="color: black; sequence: 
                     GTTATTTTAAAAACGATTCATggatccCTATTACTTTTTCTTTTTTGCCTGGCCGGCCT
                     T"
     CDS             complement(2064..2744)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2064 .. 2738 / #ff0000
                        2: 2739 .. 2741 / #ff0000 / 1a
                        3: 2742 .. 2744 / #ff0000"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     2064..2093
                     /label=Primer 1
                     /note="color: black; sequence: 
                     ctacaggaacaggtggtggcggccctcggt"
     primer_bind     complement(2405..2430)
                     /label=1122M.c4R
                     /note="color: black; sequence: 
                     tcttcttccacttccacgtagGTGACCGTGACCCAGGACTCCTCCC"
     misc_feature    2409..2417
                     /label=pt mutation
                     /note="pt mutation"
                     /note="color: #0080ff"
     primer_bind     2428..2454
                     /label=1122M.c1F
                     /note="color: black; sequence: 
                     gatcgaatatgtgtatatatacCACGCCGCCGTCCTCGAAGTTCATC"
     primer_bind     2585..2594
                     /label=1110I.c2R
                     /note="color: black; sequence: 
                     GTTATTTTAAAAACGATTCATggatccCTATTACTTTTTCTTTTTTGCCTGGCCGGCCT
                     T"
     primer_bind     complement(2712..2742)
                     /label=1122M1.c3R
                     /note="color: black; sequence: 
                     cctcttcttccacttccacgtaggtgcgctcctccaagaacgtcatcaaggag"
     primer_bind     2712..2742
                     /label=1122M1.c1F
                     /note="color: black; sequence: 
                     ctccttgatgacgttcttggaggagcgcacctacgtggaagtggaagaagagg"
     primer_bind     complement(2715..2769)
                     /label=795G2.c1R
                     /note="color: black; sequence: 
                     ccgcaacctgtctctggtgGGATCCatggtgcgctcctccaagaacgtcatcaag; 
                     added: 2022-07-19"
     primer_bind     complement(2715..2744)
                     /label=1122J.c2R
                     /note="color: black; sequence: 
                     AGATCGTGAACAACCAAGTGACttaattaaatggtgcgctcctccaagaacgtcatcaa
                     g"
     primer_bind     complement(2715..2744)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     atggtgcgctcctccaagaacgtcatcaag"
     primer_bind     2723..2769
                     /label=1122J1.c1F
                     /note="color: black; sequence: 
                     cgttcttggaggagcgcaccatttaattaacaccagagacaggttgcgg"
     primer_bind     2723..2744
                     /label=Primer 3
                     /note="color: black; sequence: 
                     cgttcttggaggagcgcaccatttaattaa"
     misc_feature    complement(2751..3297)
                     /label=Opie2 promoter
                     /note="Opie2 promoter"
                     /note="color: #0080ff; direction: LEFT"
     primer_bind     2751..2769
                     /label=CACCAGAGACAGGTTGCGG
                     /note="color: black; sequence: CACCAGAGACAGGTTGCGG"
     primer_bind     complement(2751..2769)
                     /label=1122M1.c2R
                     /note="color: black; sequence: 
                     ccgcaacctgtctctggtgttaattaaatggtatatatacacatattcgatcggatcag
                     a"
     primer_bind     complement(3081..3090)
                     /label=1110I.c2R
                     /note="color: black; sequence: 
                     GTTATTTTAAAAACGATTCATggatccCTATTACTTTTTCTTTTTTGCCTGGCCGGCCT
                     T"
     primer_bind     3223..3252
                     /label=1122G.c2R
                     /note="color: black; sequence: 
                     acctacatcgtcgacgggcccgcgtgcaaaagcgtgtttttttgc"
     primer_bind     complement(3223..3237)
                     /label=1122G.c1F
                     /note="color: black; sequence: 
                     tttgcacgcgggcccgtcgacgatgtaggtcacggtctcgaagcc"
     primer_bind     complement(3223..3237)
                     /label=F1
                     /note="color: black; sequence: tttgcacgcgggccc"
     primer_bind     complement(3268..3291)
                     /label=1110F.c1F
                     /note="color: black; sequence: 
                     tttcttgccataccatttagccgatcaattCCGCGGcatactcggtggcctccccacca
                     c"
     primer_bind     complement(3271..3291)
                     /label=CATACTCGGTGGCCTCCCCAC
                     /note="color: black; sequence: CATACTCGGTGGCCTCCCCAC"
     primer_bind     complement(3298..3327)
                     /label=Primer 4
                     /note="color: black; sequence: 
                     aattcaacgcacacttattacgtgaggtac"
     regulatory      3303..3689
                     /label=gypsy insulator
                     /note="gypsy"
                     /note="color: #ffffff"
                     /regulatory_class="insulator"
     primer_bind     3660..3690
                     /label=1110F.c1F
                     /note="color: black; sequence: 
                     tttcttgccataccatttagccgatcaattCCGCGGcatactcggtggcctccccacca
                     c"
     primer_bind     3663..3689
                     /label=1110I.c4R
                     /note="color: black; sequence: 
                     cttgccataccatttagccgatcaattgcggccgcTCGAATCGCTATCCAAGCCAGCTC
                     C"
     primer_bind     3666..3725
                     /label=1122I1.c1F
                     /note="color: black; sequence: 
                     gccataccatttagccgatcaattCTCGAGcgcgtaaaacacaatcaagtatgagtcat
                     a"
     misc_feature    3696..4777
                     /label=Hr5Ie1
                     /note="Hr5Ie1"
                     /note="color: #ff8000; direction: RIGHT"
     enhancer        3696..4178
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
                     /note="color: #a6acb3"
     promoter        4182..4773
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     4263..4291
                     /label=1140A.C1F
                     /note="color: black; sequence: 
                     ccttggcgtagaatttgtcgggtccattg"
     primer_bind     complement(4749..4816)
                     /label=795N.c1R
                     /note="color: black; sequence: 
                     tgatccgatcgaatatgtgtatatatacCATgtcacttggttgttcacgatcttgtcgc
                     c; added: 2022-05-20"
     primer_bind     4749..4816
                     /label=795N.c2F
                     /note="color: black; sequence: 
                     ggcgacaagatcgtgaacaaccaagtgacATGgtatatatacacatattcgatcggatc
                     a; added: 2022-05-20"
     primer_bind     complement(4749..4788)
                     /label=1140A.C2R
                     /note="color: black; sequence: 
                     AACAGCTCCTCGCCCTTGCTCATtttgTATTgtcacttggttgttcacgatcttgtcgc
                     c"
     primer_bind     complement(4749..4778)
                     /label=795L.c2R
                     /note="color: black; sequence: 
                     aaagaaaaaaatatgcttttaaaattacCATgtcacttggttgttcacgatcttgtcgc
                     c; added: 2022-05-20"
     primer_bind     4749..4778
                     /label=795L.c3F
                     /note="color: black; sequence: 
                     ggcgacaagatcgtgaacaaccaagtgacATGgtaattttaaaagcatatttttttctt
                     t; added: 2022-05-20"
     primer_bind     complement(4749..4778)
                     /label=795M.c1R
                     /note="color: black; sequence: 
                     CAGATCGGTTATACTATATAGTGGGTACCATgtcacttggttgttcacgatcttgtcgc
                     c; added: 2022-05-20"
     primer_bind     4749..4778
                     /label=795M.c2F
                     /note="color: black; sequence: 
                     ggcgacaagatcgtgaacaaccaagtgacATGGTACCCACTATATAGTATAACCGATCT
                     G; added: 2022-05-20"
     primer_bind     4756..4777
                     /label=1122J.c2R
                     /note="color: black; sequence: 
                     AGATCGTGAACAACCAAGTGACttaattaaatggtgcgctcctccaagaacgtcatcaa
                     g"
     primer_bind     4758..4788
                     /label=1140A.C3F
                     /note="color: black; sequence: 
                     atcgtgaacaaccaagtgacAATAcaaaATGAGCAAGGGCGAGGAGCTGTTCAC"
     primer_bind     complement(4759..4818)
                     /label=795N.c2R
                     /note="color: black; sequence: 
                     tctgatccgatcgaatatgtgtatatatacCATtttgTATTgtcacttggttgttcacg
                     a; added: 2022-08-24"
     primer_bind     4759..4818
                     /label=795N.c3F
                     /note="color: black; sequence: 
                     tcgtgaacaaccaagtgacAATAcaaaATGgtatatatacacatattcgatcggatcag
                     a; added: 2022-08-24"
     misc_feature    4782..4785
                     /label=Kozak-seq
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     4784..4818
                     /label=1122M1.c2R
                     /note="color: black; sequence: 
                     ccgcaacctgtctctggtgttaattaaatggtatatatacacatattcgatcggatcag
                     a"
     misc_feature    4786..4788
                     /label=Start codon
                     /note="color: #ff99cc"
     primer_bind     complement(4787..4810)
                     /label=1122M.c1F
                     /note="color: black; sequence: 
                     gatcgaatatgtgtatatatacCACGCCGCCGTCCTCGAAGTTCATC"
     misc_feature    4789..5019
                     /label=Ds TraF intron
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     4789..4818
                     /label=Primer 4a
                     /note="color: black; sequence: 
                     gtatatatacacatattcgatcggatcaga"
     primer_bind     complement(4987..5047)
                     /label=795N.c4R
                     /note="color: black; sequence: 
                     CCCCGGTGAACAGCTCCTCGCCCTTGCTctacgtggaagtggaagaagaggtgttaatc
                     ac; added: 2022-08-24"
     primer_bind     4987..5047
                     /label=795N.c5F
                     /note="color: black; sequence: 
                     gtgattaacacctcttcttccacttccacgtagAGCAAGGGCGAGGAGCTGTTCACCGG
                     GG; added: 2022-08-24"
     primer_bind     4997..5019
                     /label=1122M1.c3R
                     /note="color: black; sequence: 
                     cctcttcttccacttccacgtaggtgcgctcctccaagaacgtcatcaaggag"
     primer_bind     complement(4997..5019)
                     /label=1122M1.c1F
                     /note="color: black; sequence: 
                     ctccttgatgacgttcttggaggagcgcacctacgtggaagtggaagaagagg"
     primer_bind     complement(4997..5019)
                     /label=Primer 3a
                     /note="color: black; sequence: ctacgtggaagtggaagaagagg"
     primer_bind     4999..5019
                     /label=1122M.c4R
                     /note="color: black; sequence: 
                     tcttcttccacttccacgtagGTGACCGTGACCCAGGACTCCTCCC"
     primer_bind     5013..5047
                     /label=795M.c4F
                     /note="color: black; sequence: 
                     GCATTGACTAACCGCACACTCTTTTCACATAGAGCAAGGGCGAGGAGCTGTTCACCGGG
                     G; added: 2022-05-20"
     primer_bind     5017..5047
                     /label=795L.c5F
                     /note="color: black; sequence: 
                     gatactaatccatacatctatggtatctatagAGCAAGGGCGAGGAGCTGTTCACCGGG
                     G; added: 2022-05-20"
     primer_bind     complement(5017..5047)
                     /label=795L.c4R
                     /note="color: black; sequence: 
                     CCCCGGTGAACAGCTCCTCGCCCTTGCTctatagataccatagatgtatggattagtat
                     c; added: 2022-05-20"
     primer_bind     complement(5017..5047)
                     /label=795M.c3R
                     /note="color: black; sequence: 
                     CCCCGGTGAACAGCTCCTCGCCCTTGCTCTATGTGAAAAGAGTGTGCGGTTAGTCAATG
                     C; added: 2022-05-20"
     primer_bind     5019..5042
                     /label=1140A.C3F
                     /note="color: black; sequence: 
                     atcgtgaacaaccaagtgacAATAcaaaATGAGCAAGGGCGAGGAGCTGTTCAC"
     primer_bind     complement(5019..5039)
                     /label=1140A.C2R
                     /note="color: black; sequence: 
                     AACAGCTCCTCGCCCTTGCTCATtttgTATTgtcacttggttgttcacgatcttgtcgc
                     c"
     CDS             5020..5733
                     /codon_start=1
                     /label=eGFP
                     /note="color: #00ff00"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(5280..5305)
                     /label=1068D.s1R
                     /note="color: black; sequence: TGCGCTCCTGGACGTAGCCTTCGGGC"
     primer_bind     complement(5704..5755)
                     /label=1102D.C2R
                     /note="color: black; sequence: 
                     ggtatggctgattatgatctagTTACTTGTACAGCTCGTCCATGCCGAGAGT"
     primer_bind     5721..5763
                     /label=1102D.C3F
                     /note="color: black; sequence: 
                     GCTGTACAAGTAActagatcataatcagccataccacatttgt"
     misc_feature    5734..5967
                     /label=Sv40
                     /note="Sv40"
                     /note="color: #99ccff"
     polyA_signal    5843..5964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     5936..5995
                     /label=1110H.c3F
                     /note="color: black; sequence: 
                     ggtttgtccaaactcatcaatgtatcttaaagcttgtcgacgatgtaggtcacggtctc
                     g"
     primer_bind     complement(5941..6030)
                     /label=1122J1.c2R
                     /note="color: black; sequence: 
                     cccaagggcacgccctggcacccgcaccgcggcttcgagaccgtgacctacatcgtcga
                     caagctttaagatacattgatgagtttggac"
     primer_bind     complement(5943..5968)
                     /label=1140A.C4R
                     /note="color: black; sequence: 
                     tgaacattgtcagatccgagatcGGCCGGCCTAGgctttaagatacattgatgagtttg
                     g"
     misc_feature    5971..6256
                     /label=short-attP site
                     /note="color: #ff99cc"
     primer_bind     5971..6000
                     /label=1122G.c1F
                     /note="color: black; sequence: 
                     tttgcacgcgggcccgtcgacgatgtaggtcacggtctcgaagcc"
     primer_bind     complement(5971..5985)
                     /label=F2
                     /note="color: black; sequence: acctacatcgtcgac"
     primer_bind     complement(5971..5985)
                     /label=1122G.c2R
                     /note="color: black; sequence: 
                     acctacatcgtcgacgggcccgcgtgcaaaagcgtgtttttttgc"
     primer_bind     complement(6003..6032)
                     /label=Primer 5
                     /note="color: black; sequence: 
                     agcccaagggcacgccctggcacccgcacc"
     primer_bind     6225..6255
                     /label=1110H.c5F
                     /note="color: black; sequence: 
                     ggttctcgacggtcacggcgggcatgtcgac"
     primer_bind     complement(6231..6275)
                     /label=1110H.c4R
                     /note="color: black; sequence: 
                     TTTTCTTGTTATAGATATCtgtcgacatgcccgccgtgaccgtcg"
     misc_feature    complement(6257..6565)
                     /label=5' pBac transposon
                     /label=nonstandard type: transposon
                     /note="5' pBac"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(6425..6445)
                     /label=803C
                     /note="color: black; sequence: CCGTGAGGCGTGCTTGTCAAT"
     primer_bind     complement(6534..6593)
                     /label=1110H.c6R
                     /note="color: black; sequence: 
                     cgaaattaaccctcactaaaggGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG
                     C"
     primer_bind     6542..6600
                     /label=1110H.c1F
                     /note="color: black; sequence: 
                     TTTACGCAGACTATCTTTCTAGGGTTAATCcctttagtgagggttaatttcgagcttgg
                     "
     misc_feature    6566..6569
                     /label=TTAA
                     /note="color: #a6acb3"
     promoter        complement(6572..6590)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(6611..6627)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    6635..6651
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(6659..6689)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6659 .. 6665 / #ffffff / -10
                        2: 6666 .. 6683 / #ffffff
                        3: 6684 .. 6689 / #ffffff / -35"
     protein_bind    6704..6725
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(6707..6732)
                     /label=803F
                     /note="color: black; sequence: acgcaattaatgtgagttagctcact"
     rep_origin      complement(7013..7601)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(7772..8632)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7772 .. 8563 / #ccffcc
                        2: 8564 .. 8632 / #ccffcc / signal sequence
                     Cleavage site after base 8563"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8633..8737)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtagat taaccctaga aagataatca tattgtgacg tacgttaaag
      661 ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
      721 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
      781 caaacaattt atttatgttt atttatttat taaaaaaaaa caaaactttc gttgatagaa
      841 gcatcctcat cacaagatga taataagtat accatcttag ctggcttcgg tttatatgag
      901 acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc ccactggcct ggagcgactg
      961 tttttcagta cttccggtat ctcgcgtttg tttgcctgca ggtcacgtaa taagtgtgcg
     1021 ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag taaaattttg ttgcatacct
     1081 tatcaaaaaa taagtgctgc atacttttta gagaaaccaa ataatttttt attgcatacc
     1141 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tactttgacg
     1201 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcataccc gtttttaata
     1261 aaatacattg cataccctct tttaataaaa aatattgcat acgttgacga aacaaatttt
     1321 cgttgcatac ccaataaaag attattatat tgcatacctt ttcttgccat accatttagc
     1381 cgatcaattg cggccgctcg aatcgctatc caagccagct ccattgtcgg catcgtgctc
     1441 attctattga cgctggcaga tttggttttg gcgctatggg acccgttcgg ttacaacaac
     1501 atgtttccgc gcgagtttcc cgacgacatg tcgcgcacgt tcctgactgc gtactttgag
     1561 agtttcgaca acaccacgtc cagagaaatc atagagttta tgcccgagtt cttttcggaa
     1621 atggtcgaaa cggacgatga cgccacgttt gaatctctat ttcatttatt agattatgtg
     1681 gcatctttag aagttaattc cgacggccaa atgttaaact tggaggaggg tgatgaaatt
     1741 gaggattttg acgaatctac tttggtgggg caagcgttag ccactagctc gctatacact
     1801 cgcatggagt ttatgcagta cacgtttagg caaaacacac tattgtctat gaacaaagaa
     1861 aacaacaatt ttaatcaaat aatcatgggt ttatttgcaa caaacacaat tgtggcgttt
     1921 acagcatttg ttatacacac agaactcata ttttttatat ttttcgtaat cttcctaatg
     1981 atcacatttt attacataat caaagaatcg tacgaatatt ataaaacaat tgatttgtta
     2041 ttttaaaaac gattcatcct aggctacagg aacaggtggt ggcggccctc ggtgcgctcg
     2101 tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg
     2161 tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc
     2221 aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg
     2281 tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
     2341 gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc
     2401 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
     2461 tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc
     2521 ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc
     2581 agggggccgc ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc
     2641 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg
     2701 aagcgcatga actccttgat gacgttcttg gaggagcgca ccatggatcc caccagagac
     2761 aggttgcggc ggcggttgga tggcgtgggc gcgttggcgt tgttggaccg gctcatgttg
     2821 tgtcgctgta acagatgctg ttcaactgtg tttaccagat cgttgcgggc tgtatttata
     2881 ggcgcgataa gcgggacggg cgcctcgtgt ccggtcacgc gcatgagata acgcgcggct
     2941 gatatggagg cgcgtcctgt tccgataagg agttgcgtcc ggctgcggtt agcaacacag
     3001 gaagctggcg tcctgtcacg ataagacaac actcgtccgg tccgataatg tgattcgtac
     3061 gtgacaggac gcgacccgat aaggccggcc tacgtgactg ccgacacgta cttttttgca
     3121 ctgcaaaaag gttcaatgtg tggtagtgta tttggagcgt atacaacggt gtagactatt
     3181 tatgtaaaat agtctacgaa acgtagagtt tgtactatgt atgggcccgc gtgcaaaagc
     3241 gtgttttttt gcagtgcaaa aaagttggtg gtggggaggc caccgagtat gggcgcggta
     3301 cctcacgtaa taagtgtgcg ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag
     3361 taaaattttg ttgcatacct tatcaaaaaa taagtgctgc atacttttta gagaaaccaa
     3421 ataatttttt attgcatacc cgtttttaat aaaatacatt gcataccctc ttttaataaa
     3481 aaatattgca tactttgacg aaacaaattt tcgttgcata cccaataaaa gattattata
     3541 ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa aatattgcat
     3601 acgttgacga aacaaatttt cgttgcatac ccaataaaag attattatat tgcatacctt
     3661 ttcttgccat accatttagc cgatcaattc tcgagcgcgt aaaacacaat caagtatgag
     3721 tcataagctg atgtcatgtt ttgcacacgg ctcataaccg aactggcttt acgagtagaa
     3781 ttctacttgt aacgcacgat cagtggatga tgtcatttgt ttttcaaatc gagatgatgt
     3841 catgttttgc acacggctca taaactcgct ttacgagtag aattctacgt gtaacgcacg
     3901 atcgattgat gagtcatttg ttttgcaata tgatatcata caatatgact catttgtttt
     3961 tcaaaaccga acttgattta cgggtagaat tctacttgta aagcacaatc aaaaagatga
     4021 tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag aattctacgt gtaaaacaca
     4081 atcaagaaat gatgtcattt gttataaaaa taaaagctga tgtcatgttt tgcacatggc
     4141 tcataactaa actcgcttta cgggtagaat tctacgcgcg tcgatgtctt tgtgatgcgc
     4201 gcgacatttt tgtaggttat tgataaaatg aacggatacg ttgcccgaca ttatcattaa
     4261 atccttggcg tagaatttgt cgggtccatt gtccgtgtgc gctagcatgc ccgtaacgga
     4321 cctcgtactt ttggcttcaa aggttttgcg cacagacaaa atgtgccaca cttgcagctc
     4381 tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg tacttgttgt atgcaaataa
     4441 atctcgataa aggcgcggcg cgcgaatgca gctgatcacg tacgctcctc gtgttccgtt
     4501 caaggacggt gttatcgacc tcagattaat gtttatcggc cgactgtttt cgtatccgct
     4561 caccaaacgc gtttttgcat taacattgta tgtcggcgga tgttctatat ctaatttgaa
     4621 taaataaacg ataaccgcgt tggttttaga gggcataata aaagaaatat tgttatcgtg
     4681 ttcgccatta gggcagtata aattgacgtt catgttggat attgtttcag ttgcaagttg
     4741 acactggcgg cgacaagatc gtgaacaacc aagtgacaat acaaaatggt atatatacac
     4801 atattcgatc ggatcagaat tgatcaagtg caaacttttt gttgtttttt ttctagtgcc
     4861 aaattgtgtg aaatgtgaat ggcatgaagc tgaacgctgg acgagcagac aaagaagcta
     4921 ggacaactgg acactcaact gcgcattatg tggaccccaa aattcgcacc acttactacg
     4981 gctggagtga ttaacacctc ttcttccact tccacgtaga gcaagggcga ggagctgttc
     5041 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     5101 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     5161 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     5221 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     5281 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     5341 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     5401 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     5461 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     5521 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     5581 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     5641 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     5701 atcactctcg gcatggacga gctgtacaag taactagatc ataatcagcc ataccacatt
     5761 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
     5821 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
     5881 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     5941 gtccaaactc atcaatgtat cttaaagctt gtcgacgatg taggtcacgg tctcgaagcc
     6001 gcggtgcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat
     6061 ctggtccatc atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg
     6121 caccgggaag ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg
     6181 tgcgacggcg tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac
     6241 ggcgggcatg tcgacagata tctataacaa gaaaatatat atataataag ttatcacgta
     6301 agtagaacat gaaataacaa tataattatc gtatgagtta aatcttaaaa gtcacgtaaa
     6361 agataatcat gcgtcatttt gactcacgcg gtcgttatag ttcaaaatca gtgacactta
     6421 ccgcattgac aagcacgcct cacgggagct ccaagcggcg actgagatgt cctaaatgca
     6481 cagcgacgga ttcgcgctat ttagaaagag agagcaatat ttcaagaatg catgcgtcaa
     6541 ttttacgcag actatctttc tagggttaat ccctttagtg agggttaatt tcgagcttgg
     6601 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     6661 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     6721 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     6781 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     6841 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     6901 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     6961 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7021 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7081 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7141 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     7201 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     7261 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     7321 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     7381 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     7441 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     7501 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     7561 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     7621 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     7681 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     7741 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     7801 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     7861 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     7921 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     7981 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8041 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8101 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     8161 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     8221 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     8281 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     8341 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     8401 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     8461 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     8521 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     8581 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     8641 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     8701 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
//
