LOCUS       Vector_795O=pBac       11395 bp DNA     circular SYN 23-AUG-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Jul 6, 2023 from SnapGene 7.0.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..616
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          617..994
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          617..994
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          995..1002
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          8606..8891
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     source          9207..11395
                     /lab_host="Drosophila melanogaster"
                     /mol_type="other DNA"
                     /note="color: #ffffff"
                     /db_xref="taxon:2025334"
                     /organism="Cloning vector PRExpress"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     305..364
                     /label=1110D.c3F
                     /note="color: black; sequence: 
                     cgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagc
                     g"
     primer_bind     complement(587..646)
                     /label=1110H.c2R
                     /note="color: black; sequence: 
                     ACAATATGATTATCTTTCTAGGGTTAATCTactggccgtcgttttacaacgtcgtgact
                     g"
     primer_bind     592..646
                     /label=1110H.c7F
                     /note="color: black; sequence: 
                     acgacgttgtaaaacgacggccagtAGATTAACCCTAGAAAGATAATCATATTGT"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     618..683
                     /label=1110H.c6R
                     /note="color: black; sequence: 
                     cgaaattaaccctcactaaaggGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG
                     C"
     misc_feature    620..623
                     /label=TTAA
                     /note="color: #ffffff"
     misc_feature    complement(624..824)
                     /label=3' pBac transposon
                     /label=nonstandard type: transposon
                     /note="3' pBac"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(819..867)
                     /label=1110H.c8R
                     /note="color: black; sequence: 
                     cttattatcatcttgtgatgaggatgcttctatcaacgaaagtTTTGTT"
     misc_feature    825..994
                     /label=Part of Omar's piggyBacR
                     /note="color: #99ccff"
     primer_bind     889..916
                     /label=803A
                     /note="color: black; sequence: 
                     GGTTTATATGAGACGAGAGTAAGGGGTC"
     regulatory      1003..1389
                     /label=gypsy insulator
                     /note="gypsy"
                     /note="color: #ffffff"
                     /regulatory_class="insulator"
     primer_bind     complement(1003..1027)
                     /label=Primer 4
                     /note="color: black; sequence: 
                     aattcaacgcacacttattacgtgaggtac"
     primer_bind     1360..1389
                     /label=1110F.c1F
                     /note="color: black; sequence: 
                     tttcttgccataccatttagccgatcaattCCGCGGcatactcggtggcctccccacca
                     c"
     primer_bind     1363..1422
                     /label=1110I.c4R
                     /note="color: black; sequence: 
                     cttgccataccatttagccgatcaattgcggccgcTCGAATCGCTATCCAAGCCAGCTC
                     C"
     primer_bind     1366..1389
                     /label=1122I1.c1F
                     /note="color: black; sequence: 
                     gccataccatttagccgatcaattCTCGAGcgcgtaaaacacaatcaagtatgagtcat
                     a"
     3'UTR           complement(1398..2057)
                     /label=p10 3'UTR
                     /note="color: #ffff00; direction: LEFT"
     primer_bind     2034..2093
                     /label=1122J.c1F
                     /note="color: black; sequence: 
                     TTTGTTATTTTAAAAACGATTCATCCTAGGctacaggaacaggtggtggcggccctcgg
                     t"
     primer_bind     2037..2057
                     /label=1110I.c2R
                     /note="color: black; sequence: 
                     GTTATTTTAAAAACGATTCATggatccCTATTACTTTTTCTTTTTTGCCTGGCCGGCCT
                     T"
     CDS             complement(2064..2744)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2064 .. 2738 / #ff0000
                        2: 2739 .. 2741 / #ff0000 / 1a
                        3: 2742 .. 2744 / #ff0000"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     2064..2093
                     /label=Primer 1
                     /note="color: black; sequence: 
                     ctacaggaacaggtggtggcggccctcggt"
     misc_feature    2409..2417
                     /label=pt mutation
                     /note="pt mutation"
                     /note="color: #0080ff"
     primer_bind     2585..2594
                     /label=1110I.c2R
                     /note="color: black; sequence: 
                     GTTATTTTAAAAACGATTCATggatccCTATTACTTTTTCTTTTTTGCCTGGCCGGCCT
                     T"
     primer_bind     2711..2742
                     /label=1168.C2
                     /note="color: black; sequence: 
                     ACTCCTTGATGACGTTCTTGGAGGAGCGCACctgtgggcacgatg"
     primer_bind     complement(2715..2769)
                     /label=795G2.c1R
                     /note="color: black; sequence: 
                     ccgcaacctgtctctggtgGGATCCatggtgcgctcctccaagaacgtcatcaag; 
                     added: 2022-07-19"
     primer_bind     complement(2715..2744)
                     /label=1122J.c2R
                     /note="color: black; sequence: 
                     AGATCGTGAACAACCAAGTGACttaattaaatggtgcgctcctccaagaacgtcatcaa
                     g"
     primer_bind     complement(2715..2744)
                     /label=Primer 2
                     /note="color: black; sequence: 
                     atggtgcgctcctccaagaacgtcatcaag"
     primer_bind     2723..2769
                     /label=1122J1.c1F
                     /note="color: black; sequence: 
                     cgttcttggaggagcgcaccatttaattaacaccagagacaggttgcgg"
     primer_bind     2723..2744
                     /label=Primer 3
                     /note="color: black; sequence: 
                     cgttcttggaggagcgcaccatttaattaa"
     misc_feature    complement(2751..3297)
                     /label=Opie2 promoter
                     /note="Opie2 promoter"
                     /note="color: #0080ff; direction: LEFT"
     primer_bind     complement(2751..2777)
                     /label=1168.C1
                     /note="color: black; sequence: 
                     aaccgccgccgcaacctgtctctggtgATGgtaattgtaacctacattttattttatg"
     primer_bind     complement(2751..2771)
                     /label=1122N1.c1R
                     /note="color: black; sequence: 
                     cgccgcaacctgtctctggtgttaattaaATGgtaattgtaacctacattttattttat
                     g"
     primer_bind     2751..2769
                     /label=CACCAGAGACAGGTTGCGG
                     /note="color: black; sequence: CACCAGAGACAGGTTGCGG"
     primer_bind     complement(3081..3090)
                     /label=1110I.c2R
                     /note="color: black; sequence: 
                     GTTATTTTAAAAACGATTCATggatccCTATTACTTTTTCTTTTTTGCCTGGCCGGCCT
                     T"
     primer_bind     3223..3252
                     /label=1122G.c2R
                     /note="color: black; sequence: 
                     acctacatcgtcgacgggcccgcgtgcaaaagcgtgtttttttgc"
     primer_bind     complement(3223..3237)
                     /label=1122G.c1F
                     /note="color: black; sequence: 
                     tttgcacgcgggcccgtcgacgatgtaggtcacggtctcgaagcc"
     primer_bind     complement(3223..3237)
                     /label=F1
                     /note="color: black; sequence: tttgcacgcgggccc"
     primer_bind     complement(3268..3291)
                     /label=1110F.c1F
                     /note="color: black; sequence: 
                     tttcttgccataccatttagccgatcaattCCGCGGcatactcggtggcctccccacca
                     c"
     primer_bind     complement(3271..3291)
                     /label=CATACTCGGTGGCCTCCCCAC
                     /note="color: black; sequence: CATACTCGGTGGCCTCCCCAC"
     primer_bind     complement(3298..3327)
                     /label=Primer 4
                     /note="color: black; sequence: 
                     aattcaacgcacacttattacgtgaggtac"
     regulatory      3303..3689
                     /label=gypsy insulator
                     /note="gypsy"
                     /note="color: #ffffff"
                     /regulatory_class="insulator"
     primer_bind     3660..3690
                     /label=1110F.c1F
                     /note="color: black; sequence: 
                     tttcttgccataccatttagccgatcaattCCGCGGcatactcggtggcctccccacca
                     c"
     primer_bind     3663..3689
                     /label=1110I.c4R
                     /note="color: black; sequence: 
                     cttgccataccatttagccgatcaattgcggccgcTCGAATCGCTATCCAAGCCAGCTC
                     C"
     primer_bind     3666..3725
                     /label=1122I1.c1F
                     /note="color: black; sequence: 
                     gccataccatttagccgatcaattCTCGAGcgcgtaaaacacaatcaagtatgagtcat
                     a"
     misc_feature    3696..4777
                     /label=Hr5Ie1
                     /note="Hr5Ie1"
                     /note="color: #ff8000; direction: RIGHT"
     enhancer        3696..4178
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
                     /note="color: #a6acb3"
     promoter        4182..4773
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     4263..4291
                     /label=1140A.C1F
                     /note="color: black; sequence: 
                     ccttggcgtagaatttgtcgggtccattg"
     primer_bind     4538..4597
                     /label=795L.c1F
                     /note="color: black; sequence: 
                     ggccgactgttttcgtatccgctcaccaaacgcgtttttgcattaacattgtatgtcgg
                     c; added: 2022-08-23"
     primer_bind     complement(4749..4788)
                     /label=1140A.C2R
                     /note="color: black; sequence: 
                     AACAGCTCCTCGCCCTTGCTCATtttgTATTgtcacttggttgttcacgatcttgtcgc
                     c"
     primer_bind     4756..4777
                     /label=1122J.c2R
                     /note="color: black; sequence: 
                     AGATCGTGAACAACCAAGTGACttaattaaatggtgcgctcctccaagaacgtcatcaa
                     g"
     primer_bind     4758..4788
                     /label=1140A.C3F
                     /note="color: black; sequence: 
                     atcgtgaacaaccaagtgacAATAcaaaATGAGCAAGGGCGAGGAGCTGTTCAC"
     primer_bind     complement(4759..4818)
                     /label=795O.c2R
                     /note="color: black; sequence: 
                     ttcataaaataaaatgtaggttacaattacCATtttgTATTgtcacttggttgttcacg
                     a; added: 2022-08-23"
     primer_bind     4759..4818
                     /label=795O.c3F
                     /note="color: black; sequence: 
                     tcgtgaacaaccaagtgacAATAcaaaATGgtaattgtaacctacattttattttatga
                     a; added: 2022-08-23"
     misc_feature    4782..4785
                     /label=Kozak-seq
                     /note="color: #a6acb3; direction: RIGHT"
     primer_bind     4784..4816
                     /label=1122N1.c1R
                     /note="color: black; sequence: 
                     cgccgcaacctgtctctggtgttaattaaATGgtaattgtaacctacattttattttat
                     g"
     CDS             4786..8368
                     /codon_start=1
                     /label=eGFP
                     /note="color: #00ff00"
                     /translation="MVIVTYILFYEIHIKLSNKDENILG*IYMREKDTKMHVCKHQSTF
                     INDSDVDVRLDKTRLQSTY*WKNLINLSEYH*ISLQLRIYIKTSSARIGRFLRNIINCI
                     RNMQCNFTRAT*KPALD*XNGVGFGIRACDFCG*IVAGTVGKPVSWPL*IPTSTHLDVG
                     SLISIVLVSHTIYLKLLAFL*FNN**FSK*QNLLEFGREGKHCEIDE*NTHKHKNGNAS
                     TDNGEIKIITMYI*SFRWHFQMDSHICGLLIGN*FTFRYILYMCACRFISIKDLYYIYK
                     FQFQVPFAVLRGSPEQITLFLSSHVANPSETIRLQQQKRCAQA*AT*TIPFVLYLVNVT
                     PLPRIIKIQL*RTPEQD*EDNSMKTSTTGTIL*NIKI*QNMYKIYNVHIYTLCHELPGR
                     KSPWYWCSYQR*KPISSW*LQRLSES*ALKLANDMIQFYILSF*ILFSHLQLNFLNFNL
                     IILISIYHFQFHIQSIFLL*T*KDVADIEKTTLPQGLKRLS*DTHQQEQCILFLTLXQR
                     MLKSISRKKIDTV*KTKLTLNKI*QIGDHINDKNQMNYYCLFNVPWVNRKFSMWLLM*Q
                     H*HSPYLHTGIAAGVTVLGRI*IRVVSVT*NPLWWKRFSXWSILSPQFEIKSAKR*RKA
                     RGAIVVSFSPCFSTSTSIFAGRVCSLLLLYEVNADN**PQAWQVT*FRMLAIIQ*LF*H
                     *KLFITYNN*I*FKLFVRVL*TVKTTTYILFSYLKVSPRERLFKVSAGVLT*XSLELMK
                     SGIILGSTHILAKISN*TYFTVCRISKAIKNFNQQTTINKLLKGILEQQQNELKLQWQT
                     VGEINIICHLRRTTINTNVFNNVNKYNA*YTKSIHEDIFKVNSRLPTVLYLKLLSSIYY
                     *QTNKS*L*HFEXSLFQHHLGCYFYSHNQA*NK*INFAMIKRMYTNTKEMHKIKLLNKK
                     WHGKL*IYKSNDINYILLI*KIIVPTEQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGR
                     GRCHLRQADPEVHLHHRQAARALAHPRDHPDLRRAVLQPLPRPHEAARLLQVRHARRLR
                     PGAHHLLQGRRQLQDPRRGEVRGRHPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLY
                     HGRQAEERHQGELQDPPQHRGRQRAARRPLPAEHPHRRRPRAAARQPLPEHPVRPEQRP
                     QREARSHGPAGVRDRRRDHSRHGRAVQV"
     primer_bind     4786..4816
                     /label=1168.C1
                     /note="color: black; sequence: 
                     aaccgccgccgcaacctgtctctggtgATGgtaattgtaacctacattttattttatg"
     primer_bind     4787..4816
                     /label=Primer 1a
                     /note="color: black; sequence: 
                     TGgtaattgtaacctacattttattttatg"
     misc_feature    4789..7654
                     /label=AluTra-intron
                     /note="color: #c0c0c0"
     misc_feature    4789..4790
                     /label=GT
                     /note="color: #a6acb3"
     primer_bind     5668..5689
                     /label=1168-Tra-gDNA-F2
                     /note="color: black; sequence: CCTGAGCAGATAACCCTCTTTC"
     primer_bind     complement(5834..5854)
                     /label=1168-Tra-gDNA-R1
                     /note="color: black; sequence: CTTGCTCTGGAGTCCGTTAAA"
     primer_bind     6585..6605
                     /label=1168-Tra-gDNA-F3
                     /note="color: black; sequence: GGTCGTTTCGGTAACGTAGAA"
     primer_bind     complement(6784..6805)
                     /label=1168-Tra-gDNA-R2
                     /note="color: black; sequence: GTCATCAGTTGTCGGCATTTAC"
     primer_bind     complement(7625..7684)
                     /label=795O.c4R
                     /note="color: black; sequence: 
                     CACCCCGGTGAACAGCTCCTCGCCCTTGCTctgtgggcacgatgattttttatattagt
                     a; added: 2022-08-23"
     primer_bind     7625..7684
                     /label=795O.c5F
                     /note="color: black; sequence: 
                     tactaatataaaaaatcatcgtgcccacagAGCAAGGGCGAGGAGCTGTTCACCGGGGT
                     G; added: 2022-08-23"
     primer_bind     complement(7641..7654)
                     /label=1168.C2
                     /note="color: black; sequence: 
                     ACTCCTTGATGACGTTCTTGGAGGAGCGCACctgtgggcacgatg"
     primer_bind     complement(7641..7654)
                     /label=Primer 2a
                     /note="color: black; sequence: CACctgtgggcacgatg"
     misc_feature    7653..7654
                     /label=AG
                     /note="color: #a6acb3"
     primer_bind     7654..7677
                     /label=1140A.C3F
                     /note="color: black; sequence: 
                     atcgtgaacaaccaagtgacAATAcaaaATGAGCAAGGGCGAGGAGCTGTTCAC"
     primer_bind     complement(7654..7674)
                     /label=1140A.C2R
                     /note="color: black; sequence: 
                     AACAGCTCCTCGCCCTTGCTCATtttgTATTgtcacttggttgttcacgatcttgtcgc
                     c"
     primer_bind     complement(7915..7940)
                     /label=1068D.s1R
                     /note="color: black; sequence: TGCGCTCCTGGACGTAGCCTTCGGGC"
     primer_bind     complement(8002..8017)
                     /label=795O.c5F
                     /note="color: black; sequence: 
                     tactaatataaaaaatcatcgtgcccacagAGCAAGGGCGAGGAGCTGTTCACCGGGGT
                     G; added: 2022-08-23"
     primer_bind     complement(8339..8390)
                     /label=1102D.C2R
                     /note="color: black; sequence: 
                     ggtatggctgattatgatctagTTACTTGTACAGCTCGTCCATGCCGAGAGT"
     primer_bind     8356..8398
                     /label=1102D.C3F
                     /note="color: black; sequence: 
                     GCTGTACAAGTAActagatcataatcagccataccacatttgt"
     misc_feature    8369..8602
                     /label=Sv40
                     /note="Sv40"
                     /note="color: #99ccff"
     polyA_signal    8478..8599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     8571..8630
                     /label=1110H.c3F
                     /note="color: black; sequence: 
                     ggtttgtccaaactcatcaatgtatcttaaagcttgtcgacgatgtaggtcacggtctc
                     g"
     primer_bind     complement(8576..8665)
                     /label=1122J1.c2R
                     /note="color: black; sequence: 
                     cccaagggcacgccctggcacccgcaccgcggcttcgagaccgtgacctacatcgtcga
                     caagctttaagatacattgatgagtttggac"
     primer_bind     complement(8578..8603)
                     /label=1140A.C4R
                     /note="color: black; sequence: 
                     tgaacattgtcagatccgagatcGGCCGGCCTAGgctttaagatacattgatgagtttg
                     g"
     misc_feature    8606..8891
                     /label=short-attP site
                     /note="color: #ff99cc"
     primer_bind     8606..8635
                     /label=1122G.c1F
                     /note="color: black; sequence: 
                     tttgcacgcgggcccgtcgacgatgtaggtcacggtctcgaagcc"
     primer_bind     complement(8606..8620)
                     /label=F2
                     /note="color: black; sequence: acctacatcgtcgac"
     primer_bind     complement(8606..8620)
                     /label=1122G.c2R
                     /note="color: black; sequence: 
                     acctacatcgtcgacgggcccgcgtgcaaaagcgtgtttttttgc"
     primer_bind     complement(8638..8667)
                     /label=Primer 5
                     /note="color: black; sequence: 
                     agcccaagggcacgccctggcacccgcacc"
     primer_bind     8860..8890
                     /label=1110H.c5F
                     /note="color: black; sequence: 
                     ggttctcgacggtcacggcgggcatgtcgac"
     primer_bind     complement(8866..8910)
                     /label=1110H.c4R
                     /note="color: black; sequence: 
                     TTTTCTTGTTATAGATATCtgtcgacatgcccgccgtgaccgtcg"
     misc_feature    complement(8892..9200)
                     /label=5' pBac transposon
                     /label=nonstandard type: transposon
                     /note="5' pBac"
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(9060..9080)
                     /label=803C
                     /note="color: black; sequence: CCGTGAGGCGTGCTTGTCAAT"
     primer_bind     complement(9169..9228)
                     /label=1110H.c6R
                     /note="color: black; sequence: 
                     cgaaattaaccctcactaaaggGATTAACCCTAGAAAGATAGTCTGCGTAAAATTGACG
                     C"
     primer_bind     9177..9235
                     /label=1110H.c1F
                     /note="color: black; sequence: 
                     TTTACGCAGACTATCTTTCTAGGGTTAATCcctttagtgagggttaatttcgagcttgg
                     "
     misc_feature    9201..9204
                     /label=TTAA
                     /note="color: #a6acb3"
     promoter        complement(9207..9225)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(9246..9262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    9270..9286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(9294..9324)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 9294 .. 9300 / #ffffff / -10
                        2: 9301 .. 9318 / #ffffff
                        3: 9319 .. 9324 / #ffffff / -35"
     protein_bind    9339..9360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(9342..9367)
                     /label=803F
                     /note="color: black; sequence: acgcaattaatgtgagttagctcact"
     rep_origin      complement(9648..10236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(10407..11267)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 10407 .. 11198 / #ccffcc
                        2: 11199 .. 11267 / #ccffcc / signal sequence
                     Cleavage site after base 11198"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11268..11372)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtagat taaccctaga aagataatca tattgtgacg tacgttaaag
      661 ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
      721 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
      781 caaacaattt atttatgttt atttatttat taaaaaaaaa caaaactttc gttgatagaa
      841 gcatcctcat cacaagatga taataagtat accatcttag ctggcttcgg tttatatgag
      901 acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc ccactggcct ggagcgactg
      961 tttttcagta cttccggtat ctcgcgtttg tttgcctgca ggtcacgtaa taagtgtgcg
     1021 ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag taaaattttg ttgcatacct
     1081 tatcaaaaaa taagtgctgc atacttttta gagaaaccaa ataatttttt attgcatacc
     1141 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tactttgacg
     1201 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcataccc gtttttaata
     1261 aaatacattg cataccctct tttaataaaa aatattgcat acgttgacga aacaaatttt
     1321 cgttgcatac ccaataaaag attattatat tgcatacctt ttcttgccat accatttagc
     1381 cgatcaattg cggccgctcg aatcgctatc caagccagct ccattgtcgg catcgtgctc
     1441 attctattga cgctggcaga tttggttttg gcgctatggg acccgttcgg ttacaacaac
     1501 atgtttccgc gcgagtttcc cgacgacatg tcgcgcacgt tcctgactgc gtactttgag
     1561 agtttcgaca acaccacgtc cagagaaatc atagagttta tgcccgagtt cttttcggaa
     1621 atggtcgaaa cggacgatga cgccacgttt gaatctctat ttcatttatt agattatgtg
     1681 gcatctttag aagttaattc cgacggccaa atgttaaact tggaggaggg tgatgaaatt
     1741 gaggattttg acgaatctac tttggtgggg caagcgttag ccactagctc gctatacact
     1801 cgcatggagt ttatgcagta cacgtttagg caaaacacac tattgtctat gaacaaagaa
     1861 aacaacaatt ttaatcaaat aatcatgggt ttatttgcaa caaacacaat tgtggcgttt
     1921 acagcatttg ttatacacac agaactcata ttttttatat ttttcgtaat cttcctaatg
     1981 atcacatttt attacataat caaagaatcg tacgaatatt ataaaacaat tgatttgtta
     2041 ttttaaaaac gattcatcct aggctacagg aacaggtggt ggcggccctc ggtgcgctcg
     2101 tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg
     2161 tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc
     2221 aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg
     2281 tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
     2341 gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc
     2401 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
     2461 tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc
     2521 ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc
     2581 agggggccgc ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc
     2641 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg
     2701 aagcgcatga actccttgat gacgttcttg gaggagcgca ccatggatcc caccagagac
     2761 aggttgcggc ggcggttgga tggcgtgggc gcgttggcgt tgttggaccg gctcatgttg
     2821 tgtcgctgta acagatgctg ttcaactgtg tttaccagat cgttgcgggc tgtatttata
     2881 ggcgcgataa gcgggacggg cgcctcgtgt ccggtcacgc gcatgagata acgcgcggct
     2941 gatatggagg cgcgtcctgt tccgataagg agttgcgtcc ggctgcggtt agcaacacag
     3001 gaagctggcg tcctgtcacg ataagacaac actcgtccgg tccgataatg tgattcgtac
     3061 gtgacaggac gcgacccgat aaggccggcc tacgtgactg ccgacacgta cttttttgca
     3121 ctgcaaaaag gttcaatgtg tggtagtgta tttggagcgt atacaacggt gtagactatt
     3181 tatgtaaaat agtctacgaa acgtagagtt tgtactatgt atgggcccgc gtgcaaaagc
     3241 gtgttttttt gcagtgcaaa aaagttggtg gtggggaggc caccgagtat gggcgcggta
     3301 cctcacgtaa taagtgtgcg ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag
     3361 taaaattttg ttgcatacct tatcaaaaaa taagtgctgc atacttttta gagaaaccaa
     3421 ataatttttt attgcatacc cgtttttaat aaaatacatt gcataccctc ttttaataaa
     3481 aaatattgca tactttgacg aaacaaattt tcgttgcata cccaataaaa gattattata
     3541 ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa aatattgcat
     3601 acgttgacga aacaaatttt cgttgcatac ccaataaaag attattatat tgcatacctt
     3661 ttcttgccat accatttagc cgatcaattc tcgagcgcgt aaaacacaat caagtatgag
     3721 tcataagctg atgtcatgtt ttgcacacgg ctcataaccg aactggcttt acgagtagaa
     3781 ttctacttgt aacgcacgat cagtggatga tgtcatttgt ttttcaaatc gagatgatgt
     3841 catgttttgc acacggctca taaactcgct ttacgagtag aattctacgt gtaacgcacg
     3901 atcgattgat gagtcatttg ttttgcaata tgatatcata caatatgact catttgtttt
     3961 tcaaaaccga acttgattta cgggtagaat tctacttgta aagcacaatc aaaaagatga
     4021 tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag aattctacgt gtaaaacaca
     4081 atcaagaaat gatgtcattt gttataaaaa taaaagctga tgtcatgttt tgcacatggc
     4141 tcataactaa actcgcttta cgggtagaat tctacgcgcg tcgatgtctt tgtgatgcgc
     4201 gcgacatttt tgtaggttat tgataaaatg aacggatacg ttgcccgaca ttatcattaa
     4261 atccttggcg tagaatttgt cgggtccatt gtccgtgtgc gctagcatgc ccgtaacgga
     4321 cctcgtactt ttggcttcaa aggttttgcg cacagacaaa atgtgccaca cttgcagctc
     4381 tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg tacttgttgt atgcaaataa
     4441 atctcgataa aggcgcggcg cgcgaatgca gctgatcacg tacgctcctc gtgttccgtt
     4501 caaggacggt gttatcgacc tcagattaat gtttatcggc cgactgtttt cgtatccgct
     4561 caccaaacgc gtttttgcat taacattgta tgtcggcgga tgttctatat ctaatttgaa
     4621 taaataaacg ataaccgcgt tggttttaga gggcataata aaagaaatat tgttatcgtg
     4681 ttcgccatta gggcagtata aattgacgtt catgttggat attgtttcag ttgcaagttg
     4741 acactggcgg cgacaagatc gtgaacaacc aagtgacaat acaaaatggt aattgtaacc
     4801 tacattttat tttatgaaat tcatataaaa ctaagcaata aggacgaaaa catactcggt
     4861 tgaatttata tgcgtgaaaa agatacaaaa atgcacgttt gcaaacacca atcaacattc
     4921 atcaatgata gtgatgtgga cgtcagattg gacaaaacta ggctacaatc aacatactaa
     4981 tggaagaatc ttataaactt aagtgagtac cattagataa gtctccaact aagaatttat
     5041 atcaagacga gcagtgctag aataggtcgt tttttgagaa atataattaa ctgtatcaga
     5101 aatatgcagt gcaattttac tcgagctact tagaagcctg ctctagatta aaakaatggg
     5161 gttggctttg gaattagggc atgcgatttt tgtgggtaaa tcgtggctgg taccgtagga
     5221 aagccggtgt cctggcctct ctaaataccc acttcgaccc acttggatgt cggaagcttg
     5281 atctcaattg ttttagtttc acacacaata tatttaaaac tgctggcatt tctgtaattt
     5341 aataattaat aattttccaa gtagcaaaac ttgcttgagt ttggcagaga aggaaagcat
     5401 tgcgagatag atgagtaaaa cacacacaaa cataagaatg ggaatgctag cacagataat
     5461 ggagaaataa aaattattac tatgtatatt taatcgtttc gatggcattt tcaaatggat
     5521 tctcatattt gcggcctatt gattggtaat taatttacct ttcgttacat attatatatg
     5581 tgtgcctgta ggtttatatc tataaaggat ttatattata tatacaaatt tcaatttcaa
     5641 gtacccttcg cggttctccg gggctcacct gagcagataa ccctctttct ctcatctcat
     5701 gtagcgaatc cgtccgaaac catcagacta caacaacaaa agcggtgcgc acaggcataa
     5761 gcaacgtaga caatcccatt tgtcctttac cttgtaaacg taacacccct tccgagaatc
     5821 attaagattc aactttaacg gactccagag caagattgag aagataactc gatgaagaca
     5881 tcgaccacag ggacaattct ttagaatatc aaaatatagc agaatatgta caaaatatat
     5941 aatgtacata tatataccct ctgtcacgag ctgcctggca ggaaatcacc ctggtattgg
     6001 tgctcttatc agaggtgaaa accgattagt agctggtgac ttcaacgctt atcagaatct
     6061 taagcactca agcttgccaa tgatatgata caattttata ttctctcatt ttgaattcta
     6121 ttttctcatt tgcaacttaa ttttctcaat ttcaatttaa tcattctcat ttcaatatat
     6181 cattttcaat ttcatattca atctattttc ttattatgaa cttgaaaaga tgtagctgac
     6241 attgaaaaaa ccactctccc acagggactg aagaggttgt cctaagacac acatcaacaa
     6301 gagcaatgca ttttgttttt gactttggwc caaagaatgt taaaatctat aagcagaaag
     6361 aagattgaca ctgtatagaa aacaaaatta acgttaaaca aaatttgaca aattggagac
     6421 catataaatg ataaaaatca aatgaactac tactgcctgt tcaatgtacc ttgggtgaac
     6481 cgcaaatttt ctatgtggtt gttgatgtag cagcattaac attccccata tttacatacg
     6541 gggattgctg ctggagtgac agtccttggc cggatataaa tccgggtcgt ttcggtaacg
     6601 tagaacccac tgtggtggaa acgtttttct wtgtggtcga tactctctcc tcaattcgag
     6661 atcaaatctg caaaaagatg acgtaaagca aggggtgcca tagtagtgtc cttttcccct
     6721 tgcttttcaa cttctacatc catctttgcc ggaagagtct gctccctact cttactctat
     6781 gaggtaaatg ccgacaactg atgaccacag gcttggcagg ttacttaatt ccgaatgtta
     6841 gcgatcatac aataattatt ttaacactaa aaattgttta ttacttacaa taattaaatt
     6901 taatttaaat tgtttgtgag ggttttgtaa actgttaaaa caactacata catcttattt
     6961 tcatatctta aagtttcacc tagggagcgt ttatttaaag tctctgctgg agtcttgact
     7021 tgagkgagtt tagagctgat gaaatcaggg attatattgg gttcaaccca tattttagct
     7081 aaaatatcca actgaacata ttttacagtt tgtcgaataa gcaaagcaat taaaaacttc
     7141 aatcaacaaa ctacaatcaa caaactatta aagggaatac tggaacaaca acaaaatgaa
     7201 ctcaaactac aatggcaaac tgttggggaa ataaacatca tttgccacct gaggagraca
     7261 acaatcaaca caaacgtttt taacaatgta aataaatata atgcttgata tacgaaatca
     7321 atacatgaag acatttttaa agttaactct cgactaccta cagtgcttta tttaaaatta
     7381 ctttcttcga tttattatta gcaaacaaac aaaagctagc tttaacattt tgaaarttca
     7441 ctttttcagc accatttggg ttgctatttt tattcacata atcaagcata aaacaaataa
     7501 attaattttg caatgattaa acgtatgtac acaaatacga aagaaatgca taagataaaa
     7561 ttactcaaca aaaaatggca tggaaaattg tagatataca aatcaaatga tattaattat
     7621 atactactaa tataaaaaat catcgtgccc acagagcaag ggcgaggagc tgttcaccgg
     7681 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     7741 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     7801 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     7861 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     7921 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     7981 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     8041 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     8101 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     8161 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     8221 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     8281 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     8341 tctcggcatg gacgagctgt acaagtaact agatcataat cagccatacc acatttgtag
     8401 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     8461 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8521 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8581 aactcatcaa tgtatcttaa agcttgtcga cgatgtaggt cacggtctcg aagccgcggt
     8641 gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt
     8701 ccatcatgat gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg
     8761 ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga
     8821 cggcgtcggc gggtgcggat acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg
     8881 gcatgtcgac agatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag
     8941 aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata
     9001 atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca
     9061 ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg
     9121 acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta
     9181 cgcagactat ctttctaggg ttaatccctt tagtgagggt taatttcgag cttggcgtaa
     9241 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     9301 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     9361 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     9421 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     9481 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     9541 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9601 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9661 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9721 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9781 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9841 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9901 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9961 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    10021 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    10081 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    10141 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    10201 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    10261 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    10321 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    10381 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    10441 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    10501 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    10561 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10621 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10681 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10741 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10801 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10861 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10921 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10981 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    11041 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    11101 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    11161 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    11221 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    11281 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    11341 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
