LOCUS       Exported                8451 bp ds-DNA     linear   UNA 08-FEB-2023
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..8451
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    831..1541
                     /locus_tag="electra1"
                     /label=electra1
     misc_feature    1548..1622
                     /locus_tag="T2A"
                     /label=T2A
     misc_feature    1623..2333
                     /locus_tag="mCherry"
                     /label=mCherry
     misc_feature    4706..5505
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5937..6797
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7880..7909
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc atggtgtcta
      841 agggcgaaga gctgatcgag gaaaatatgc gtatgaaggt ggtcatggaa ggttcggtca
      901 acggccacca attcaaatgc acaggtgaag gagaaggcag accgtacgag ggagtgcaaa
      961 ccatgaggat caaagtcatc gagggaggac ccctgccatt tgcctttgac attcttgcca
     1021 cgtcgttcct gttcggcagc aagaccttta tcaagtaccc ggccgacatc cctgatttct
     1081 tcaaacagtc ctttcctgag ggctttactt gggaaagagt tacgagatac gaagatggtg
     1141 gagtcgtcac cgtcacgcag gacaccagcc ttgaggatgg ggggctcgtc tacaacgtca
     1201 aggtcagagg ggtaaacttt ccctccaatg gtcccgtgat gcagaagaag accgagggtt
     1261 gggagccttt cacagagatg atgtatccag caaatggtgg tctgagagga tataccgaca
     1321 tcgcactgaa agttgatggt gatggccatc tgcacgccaa catcgtgaca acttacaggt
     1381 caaaaaagac cgtcggggac atcaagatgc ccggtgtcca tgccgttgat taccgcctgg
     1441 aaagggtcga agagagtgac aatgaaacct acgtagtgct aagagaagtg gcagttgcca
     1501 aatacagcaa ccttggtggt ggcatggacg agctgtacaa gtccggagag ggcagaggaa
     1561 gtcttctaac atgcggtgac gtggaggaga atcccggccc tggggatcca ccggtcgcca
     1621 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
     1681 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
     1741 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
     1801 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
     1861 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
     1921 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
     1981 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
     2041 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
     2101 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
     2161 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
     2221 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     2281 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag tagcggccgc
     2341 acccagcttt cttgtacaaa gtggtgtagt taattcatga tggccgctgc aggtcgacct
     2401 cgaggggggg cccggtaccc aattcgccct atagtgagtc gtattacgcg cggatccagc
     2461 tttggacttc ttcgccagag gtttggtcaa gtctccaatc aaggttgtcg gcttgtctac
     2521 cttgccagaa atttacgaaa agatggaaaa gggtcaaatc gttggtagat acgttgttga
     2581 cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa
     2641 aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgan aattcttatt
     2701 cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct
     2761 tattgaccac acctctaccg gcatgccaat tcactggccg tcgttttaca acgtcgtgac
     2821 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     2881 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2941 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     3001 ataatcggat cgtacttgtt acccatcatt gaattttgaa catccgaacc tgggagtttt
     3061 ccctgaaaca gatagtatat ttgaacctgt ataataatat atagtctagc gctttacgga
     3121 agacaatgta tgtatttcgg ttcctggaga aactattgca tctattgcat aggtaatctt
     3181 gcacgtcgca tccccggttc attttctgcg tttccatctt gcacttcaat agcatatctt
     3241 tgttaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat
     3301 ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct
     3361 attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg
     3421 ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga
     3481 gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga
     3541 gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat
     3601 aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact
     3661 ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt
     3721 actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac
     3781 cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg
     3841 tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt
     3901 tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc
     3961 taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc
     4021 aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa
     4081 gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac
     4141 agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa
     4201 gcgctctgaa gttcctatac tttctagcta gagaatagga acttcggaat aggaacttca
     4261 aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca
     4321 ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg
     4381 gcatagtgcg tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa
     4441 ggtagtctag tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt
     4501 cagcactacc ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct
     4561 tcaatgctat catttccttt gatattggat cgatccgatg ataagctgtc aaacatgaga
     4621 attgggtaat aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc
     4681 atttacttat aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca
     4741 gcctgctttt ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc
     4801 tcttccaaca ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg
     4861 ttgacccaat gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc
     4921 gtaaccttca tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt
     4981 gtcgctcttc gcaatgtcaa cagtaccctt agtatattct ccagtagata gggagccctt
     5041 gcatgacaat tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttctgccgc
     5101 ctgcttcaaa ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc
     5161 ccattctgct attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc
     5221 agcaaatttt ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg
     5281 cttaactgtg ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca
     5341 aattttggga cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa
     5401 tgaagcacac aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg
     5461 actaggatga gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt
     5521 cctgcatgtt tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc
     5581 aacactacat atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt
     5641 ctgttcggag attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa aaaaagaata
     5701 aaaaaaaaat gatgaattga aaagctaatt cttgaagacg aaagggcctc gtgatacgcc
     5761 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5821 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5881 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5941 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6001 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6061 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6121 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6181 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6241 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6301 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6361 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6421 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6481 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6541 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6601 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6661 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6721 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6781 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6841 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6901 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6961 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7021 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7081 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7141 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7201 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7261 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7321 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7381 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7441 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7501 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7561 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7621 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7681 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     7741 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     7801 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7861 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     7921 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttaccg
     7981 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     8041 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     8101 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8161 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8221 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     8281 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     8341 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     8401 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg c
//
