LOCUS       Exported                8457 bp ds-DNA     linear   UNA 03-MAR-2023
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8457)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..8457
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    831..1547
                     /locus_tag="Achilles"
                     /label=Achilles
     regulatory      831..834
                     /regulatory_class="other"
                     /locus_tag="vertebrate consensus sequence for strong
                     initiation  of translation (Kozak, 1987)"
                     /label=vertebrate consensus sequence for strong
                     initia...
                     /label=vertebrate consensus sequence for strong
                     initiation  of translation (Kozak, 1987)
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     primer_bind     complement(876..897)
                     /locus_tag="EGFP-N"
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1137..1156)
                     /locus_tag="EXFP-R"
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1296..1547
                     /codon_start=1
                     /locus_tag="VC155"
                     /product="C-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSFLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1484..1505
                     /locus_tag="EGFP-C"
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    1554..1628
                     /locus_tag="T2A"
                     /label=T2A
     misc_feature    1629..2339
                     /locus_tag="mCherry"
                     /label=mCherry
     misc_feature    4712..5511
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5943..6803
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7886..7915
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc atggtgagca
      841 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
      901 acggccacaa gttcagcgtg cgcggcgagg gcgagggcga tgccaccatc ggcaagctga
      961 ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     1021 ccctgggcta cggcctggcc gtcttcgccc gctaccccga ccacatgaag cagcacgact
     1081 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     1141 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     1201 tcgagctgaa gggctccggc ttcaaggagg acggcaacat cctggggcac aagctggagt
     1261 acaacttcaa cagccacaac gtctatatca ccgccgacaa gcagaagaac ggcatcaagg
     1321 ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc
     1381 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct
     1441 accagtcctt cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     1501 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggagagggca
     1561 gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctggg gatccaccgg
     1621 tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     1681 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     1741 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     1801 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     1861 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     1921 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     1981 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     2041 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     2101 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     2161 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     2221 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     2281 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtagc
     2341 ggccgcaccc agctttcttg tacaaagtgg tgtagttaat tcatgatggc cgctgcaggt
     2401 cgacctcgag ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgga
     2461 tccagctttg gacttcttcg ccagaggttt ggtcaagtct ccaatcaagg ttgtcggctt
     2521 gtctaccttg ccagaaattt acgaaaagat ggaaaagggt caaatcgttg gtagatacgt
     2581 tgttgacact tctaaataag cgaatttctt atgatttatg atttttatta ttaaataagt
     2641 tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacganaatt
     2701 cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt
     2761 cgctcttatt gaccacacct ctaccggcat gccaattcac tggccgtcgt tttacaacgt
     2821 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     2881 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     2941 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     3001 caccgcataa tcggatcgta cttgttaccc atcattgaat tttgaacatc cgaacctggg
     3061 agttttccct gaaacagata gtatatttga acctgtataa taatatatag tctagcgctt
     3121 tacggaagac aatgtatgta tttcggttcc tggagaaact attgcatcta ttgcataggt
     3181 aatcttgcac gtcgcatccc cggttcattt tctgcgtttc catcttgcac ttcaatagca
     3241 tatctttgtt aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc
     3301 gctaattttt caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa
     3361 agcgctattt taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg
     3421 agagcgctaa tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac
     3481 gcgagagcgc tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca
     3541 tcccgagagc gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg
     3601 ctctataatg cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag
     3661 gctactttgg tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta
     3721 ctgattacta gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt
     3781 ctataccgat gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt
     3841 cattggtcag aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga
     3901 aatgtttaca ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt
     3961 tttgtctaaa gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc
     4021 aagttcaagg agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat
     4081 agcaaagaga tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct
     4141 cgttacagtc cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt
     4201 tcaaaagcgc tctgaagttc ctatactttc tagctagaga ataggaactt cggaatagga
     4261 acttcaaagc gtttccgaaa acgagcgctt ccgaaaatgc aacgcgagct gcgcacatac
     4321 agctcactgt tcacgtcgca cctatatctg cgtgttgcct gtatatatat atacatgaga
     4381 agaacggcat agtgcgtgtt tatgcttaaa tgcgtactta tatgcgtcta tttatgtagg
     4441 atgaaaggta gtctagtacc tcctgtgata ttatcccatt ccatgcgggg tatcgtatgc
     4501 ttccttcagc actacccttt agctgttcta tatgctgcca ctcctcaatt ggattagtct
     4561 catccttcaa tgctatcatt tcctttgata ttggatcgat ccgatgataa gctgtcaaac
     4621 atgagaattg ggtaataact gatataatta aattgaagct ctaatttgtg agtttagtat
     4681 acatgcattt acttataata cagtttttta gttttgctgg ccgcatcttc tcaaatatgc
     4741 ttcccagcct gcttttctgt aacgttcacc ctctacctta gcatcccttc cctttgcaaa
     4801 tagtcctctt ccaacaataa taatgtcaga tcctgtagag accacatcat ccacggttct
     4861 atactgttga cccaatgcgt ctcccttgtc atctaaaccc acaccgggtg tcataatcaa
     4921 ccaatcgtaa ccttcatctc ttccacccat gtctctttga gcaataaagc cgataacaaa
     4981 atctttgtcg ctcttcgcaa tgtcaacagt acccttagta tattctccag tagataggga
     5041 gcccttgcat gacaattctg ctaacatcaa aaggcctcta ggttcctttg ttacttcttc
     5101 tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc acaccgtgtg cattcgtaat
     5161 gtctgcccat tctgctattc tgtatacacc cgcagagtac tgcaatttga ctgtattacc
     5221 aatgtcagca aattttctgt cttcgaagag taaaaaattg tacttggcgg ataatgcctt
     5281 tagcggctta actgtgccct ccatggaaaa atcagtcaag atatccacat gtgtttttag
     5341 taaacaaatt ttgggaccta atgcttcaac taactccagt aattccttgg tggtacgaac
     5401 atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg atattaaata gcttggcagc
     5461 aacaggacta ggatgagtag cagcacgttc cttatatgta gctttcgaca tgatttatct
     5521 tcgtttcctg catgtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt
     5581 ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc
     5641 ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa
     5701 agaataaaaa aaaaatgatg aattgaaaag ctaattcttg aagacgaaag ggcctcgtga
     5761 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     5821 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5881 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5941 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6001 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6061 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6121 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6181 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6241 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6301 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6361 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     6421 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     6481 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     6541 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     6601 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     6661 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     6721 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6781 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6841 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6901 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6961 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7021 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7081 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7141 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7201 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7261 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7321 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7381 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     7441 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     7501 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     7561 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     7621 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     7681 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     7741 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     7801 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     7861 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     7921 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     7981 ttaccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     8041 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     8101 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     8161 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     8221 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     8281 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     8341 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     8401 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgc
//
