LOCUS       Exported                8441 bp ds-DNA     linear   UNA 07-FEB-2023
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..8441
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    683..709
                     /locus_tag="primer 7"
                     /label=primer 7
     misc_feature    838..1551
                     /locus_tag="yeast optimized mTq2d9 from DBO159"
                     /label=yeast optimized mTq2d9 from DBO159
     misc_feature    1552..1560
                     /locus_tag="linker"
                     /label=linker
     misc_feature    1561..1617
                     /locus_tag="P2A"
                     /label=P2A
     misc_feature    1624..2322
                     /locus_tag="ymScarletI"
                     /label=ymScarletI
     misc_feature    4696..5495
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5927..6787
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7870..7899
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgaa
      781 ctagtgaatt cgcggccatc acaagtttgt acaaaaaagc aggcttatgc tagcatggtt
      841 agtaaaggtg aagaattgtt cactggtgta gtgccaattt tagtagaatt agatggtgac
      901 gttaacggtc ataagttctc tgtaagcggt gaaggtgaag gtgacgcaac atacggtaaa
      961 ttaactttga aattcatttg tactacaggt aaattaccag ttccatggcc aactttggtt
     1021 actactttgt cttggggtgt tcaatgtttc gctagatacc cagatcatat gaagcaacat
     1081 gatttcttta agtctgctat gccagaaggt tacgttcaag aaagaactat cttcttcaag
     1141 gatgatggta actataagac tagagctgaa gttaagttcg aaggagatac tttagttaat
     1201 agaattgaat tgaagggtat tgattttaag gaagatggta atattttggg tcataagttg
     1261 gaatataatt acttctctga taacgtttac attactgctg ataagcaaaa gaacggtatt
     1321 aaggcgaact tcaagattag acataacatt gaagatggtg gtgttcaatt agctgatcat
     1381 taccaacaaa acactccaat tggtgacggt ccagttttgt tgccagataa ccattacttg
     1441 tctactcaat ctaagttgtc taaggatcca aacgaaaaga gagatcatat ggttttgttg
     1501 gaattcgtta ctgctgctgg gatcacttta ggtatggatg aattgtataa aggttctggt
     1561 gcaacaaatt tctcattgtt gaagcaggcg ggagatgtgg aggagaaccc tggacctccc
     1621 gggatggtta gtaaaggtga agctgttata aaagaattca tgaggtttaa agttcatatg
     1681 gaaggttcaa tgaatggtca tgaatttgaa attgaaggtg aaggtgaagg tagaccatat
     1741 gaaggtacac aaactgctaa attgaaagtt actaaaggtg gtccattacc attttcttgg
     1801 gatattttgt ctccacaatt catgtatggt tctagagctt ttattaagca tccagctgat
     1861 attccagatt attataaaca atcttttcct gaaggtttta aatgggaaag agttatgaat
     1921 ttcgaagatg gtggtgctgt tactgttact caagatactt ctttggaaga tggtacttta
     1981 atctataaag ttaaattgag aggtactaat tttccaccag atggtccagt tatgcaaaag
     2041 aaaactatgg gttgggaagc atctactgaa agattgtatc cagaagatgg tgttttgaaa
     2101 ggagacatta agatggcttt gagattgaaa gatggtggtc ggtacctggc cgacttcaag
     2161 accacctata aagctaaaaa accagttcaa atgccaggtg catataatgt tgatagaaag
     2221 ttagatataa cgtcgcataa cgaggactat actgtagttg aacaatatga acgtagtgaa
     2281 ggtagacata gtaccggagg aatggatgaa ttgtataaat aagcggccgc acccagcttt
     2341 cttgtacaaa gtggtgtagt taattcatga tggccgctgc aggtcgacct cgaggggggg
     2401 cccggtaccc aattcgccct atagtgagtc gtattacgcg cggatccagc tttggacttc
     2461 ttcgccagag gtttggtcaa gtctccaatc aaggttgtcg gcttgtctac cttgccagaa
     2521 atttacgaaa agatggaaaa gggtcaaatc gttggtagat acgttgttga cacttctaaa
     2581 taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt
     2641 gtatacaaat tttaaagtga ctcttaggtt ttaaaacgan aattcttatt cttgagtaac
     2701 tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct tattgaccac
     2761 acctctaccg gcatgccaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     2821 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     2881 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     2941 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataatcggat
     3001 cgtacttgtt acccatcatt gaattttgaa catccgaacc tgggagtttt ccctgaaaca
     3061 gatagtatat ttgaacctgt ataataatat atagtctagc gctttacgga agacaatgta
     3121 tgtatttcgg ttcctggaga aactattgca tctattgcat aggtaatctt gcacgtcgca
     3181 tccccggttc attttctgcg tttccatctt gcacttcaat agcatatctt tgttaacgaa
     3241 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     3301 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
     3361 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc
     3421 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt
     3481 accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt
     3541 tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct
     3601 cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta
     3661 ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag
     3721 ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat
     3781 tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt
     3841 atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg
     3901 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
     3961 tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa
     4021 aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt
     4081 tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc
     4141 gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa
     4201 gttcctatac tttctagcta gagaatagga acttcggaat aggaacttca aagcgtttcc
     4261 gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt
     4321 cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg
     4381 tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa ggtagtctag
     4441 tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc
     4501 ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat
     4561 catttccttt gatattggat cgatccgatg ataagctgtc aaacatgaga attgggtaat
     4621 aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat
     4681 aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt
     4741 ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca
     4801 ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat
     4861 gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca
     4921 tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc
     4981 gcaatgtcaa cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat
     5041 tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa
     5101 ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct
     5161 attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt
     5221 ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg
     5281 ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga
     5341 cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac
     5401 aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga
     5461 gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt cctgcatgtt
     5521 tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat
     5581 atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag
     5641 attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat
     5701 gatgaattga aaagctaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     5761 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5821 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5881 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5941 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6001 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6061 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6121 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6181 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6241 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6301 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6361 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6421 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6481 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6541 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6601 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6661 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6721 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6781 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6841 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6901 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6961 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7021 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7081 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7141 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7201 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7261 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7321 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7381 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7441 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7501 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7561 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7621 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7681 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7741 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     7801 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     7861 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     7921 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttaccg catcaggaaa
     7981 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     8041 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     8101 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     8161 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     8221 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     8281 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     8341 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     8401 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg c
//
