LOCUS       Exported                8444 bp ds-DNA     linear   UNA 15-FEB-2023
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     >pDRf1-GW sequence 8606 bps
FEATURES             Location/Qualifiers
     source          1..8444
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     misc_feature    1..774
                     /locus_tag="pma1 promoter"
                     /label=pma1 promoter
     misc_feature    683..709
                     /locus_tag="primer 7"
                     /label=primer 7
     primer_bind     826..861
                     /locus_tag="Primer 130"
                     /label=Primer 130
     misc_feature    838..1551
                     /locus_tag="yeast optimized mTq2d9 from DBO159"
                     /label=yeast optimized mTq2d9 from DBO159
     misc_feature    1552..1560
                     /locus_tag="linker"
                     /label=linker
     misc_feature    1561..1620
                     /locus_tag="E2A"
                     /label=E2A
     misc_feature    1627..2325
                     /locus_tag="ymScarletI"
                     /label=ymScarletI
     misc_feature    4699..5498
                     /locus_tag="URA3"
                     /label=URA3
     misc_feature    5930..6790
                     /locus_tag="amp resistentie"
                     /label=amp resistentie
     misc_feature    7873..7902
                     /locus_tag="lac promotor"
                     /label=lac promotor
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgaa
      781 ctagtgaatt cgcggccatc acaagtttgt acaaaaaagc aggcttatgc tagcatggtt
      841 agtaaaggtg aagaattgtt cactggtgta gtgccaattt tagtagaatt agatggtgac
      901 gttaacggtc ataagttctc tgtaagcggt gaaggtgaag gtgacgcaac atacggtaaa
      961 ttaactttga aattcatttg tactacaggt aaattaccag ttccatggcc aactttggtt
     1021 actactttgt cttggggtgt tcaatgtttc gctagatacc cagatcatat gaagcaacat
     1081 gatttcttta agtctgctat gccagaaggt tacgttcaag aaagaactat cttcttcaag
     1141 gatgatggta actataagac tagagctgaa gttaagttcg aaggagatac tttagttaat
     1201 agaattgaat tgaagggtat tgattttaag gaagatggta atattttggg tcataagttg
     1261 gaatataatt acttctctga taacgtttac attactgctg ataagcaaaa gaacggtatt
     1321 aaggcgaact tcaagattag acataacatt gaagatggtg gtgttcaatt agctgatcat
     1381 taccaacaaa acactccaat tggtgacggt ccagttttgt tgccagataa ccattacttg
     1441 tctactcaat ctaagttgtc taaggatcca aacgaaaaga gagatcatat ggttttgttg
     1501 gaattcgtta ctgctgctgg gatcacttta ggtatggatg aattgtataa aggttctggt
     1561 ggcgctacca atttctcact actaaaactg gcgggtgacg tcgaattgaa tcccggccct
     1621 cccgggatgg ttagtaaagg tgaagctgtt ataaaagaat tcatgaggtt taaagttcat
     1681 atggaaggtt caatgaatgg tcatgaattt gaaattgaag gtgaaggtga aggtagacca
     1741 tatgaaggta cacaaactgc taaattgaaa gttactaaag gtggtccatt accattttct
     1801 tgggatattt tgtctccaca attcatgtat ggttctagag cttttattaa gcatccagct
     1861 gatattccag attattataa acaatctttt cctgaaggtt ttaaatggga aagagttatg
     1921 aatttcgaag atggtggtgc tgttactgtt actcaagata cttctttgga agatggtact
     1981 ttaatctata aagttaaatt gagaggtact aattttccac cagatggtcc agttatgcaa
     2041 aagaaaacta tgggttggga agcatctact gaaagattgt atccagaaga tggtgttttg
     2101 aaaggagaca ttaagatggc tttgagattg aaagatggtg gtcggtacct ggccgacttc
     2161 aagaccacct ataaagctaa aaaaccagtt caaatgccag gtgcatataa tgttgataga
     2221 aagttagata taacgtcgca taacgaggac tatactgtag ttgaacaata tgaacgtagt
     2281 gaaggtagac atagtaccgg aggaatggat gaattgtata aataagcggc cgcacccagc
     2341 tttcttgtac aaagtggtgt agttaattca tgatggccgc tgcaggtcga cctcgagggg
     2401 gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcggatcc agctttggac
     2461 ttcttcgcca gaggtttggt caagtctcca atcaaggttg tcggcttgtc taccttgcca
     2521 gaaatttacg aaaagatgga aaagggtcaa atcgttggta gatacgttgt tgacacttct
     2581 aaataagcga atttcttatg atttatgatt tttattatta aataagttat aaaaaaaata
     2641 agtgtataca aattttaaag tgactcttag gttttaaaac ganaattctt attcttgagt
     2701 aactctttcc tgtaggtcag gttgctttct caggtatagc atgaggtcgc tcttattgac
     2761 cacacctcta ccggcatgcc aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
     2821 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
     2881 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
     2941 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcataatcg
     3001 gatcgtactt gttacccatc attgaatttt gaacatccga acctgggagt tttccctgaa
     3061 acagatagta tatttgaacc tgtataataa tatatagtct agcgctttac ggaagacaat
     3121 gtatgtattt cggttcctgg agaaactatt gcatctattg cataggtaat cttgcacgtc
     3181 gcatccccgg ttcattttct gcgtttccat cttgcacttc aatagcatat ctttgttaac
     3241 gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct aatttttcaa
     3301 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc gctattttac
     3361 caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga gcgctaattt
     3421 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat
     3481 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct
     3541 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag
     3601 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt
     3661 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg
     3721 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg
     3781 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa
     3841 attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt
     3901 tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag
     3961 taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc
     4021 gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac
     4081 ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt tacagtccgg
     4141 tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca aaagcgctct
     4201 gaagttccta tactttctag ctagagaata ggaacttcgg aataggaact tcaaagcgtt
     4261 tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc tcactgttca
     4321 cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga acggcatagt
     4381 gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg aaaggtagtc
     4441 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact
     4501 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc
     4561 tatcatttcc tttgatattg gatcgatccg atgataagct gtcaaacatg agaattgggt
     4621 aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact
     4681 tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct
     4741 tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca
     4801 acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc
     4861 aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct
     4921 tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc
     4981 ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac
     5041 aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc
     5101 aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct
     5161 gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat
     5221 tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact
     5281 gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg
     5341 ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca
     5401 cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga
     5461 tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg tttcctgcat
     5521 gtttttgttc tgtgcagttg ggttaagaat actgggcaat ttcatgtttc ttcaacacta
     5581 catatgcgta tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg
     5641 gagattaccg aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa
     5701 aatgatgaat tgaaaagcta attcttgaag acgaaagggc ctcgtgatac gcctattttt
     5761 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     5821 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     5881 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     5941 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     6001 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     6061 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     6121 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     6181 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     6241 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     6301 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     6361 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     6421 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     6481 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     6541 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     6601 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     6661 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     6721 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     6781 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     6841 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     6901 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     6961 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     7021 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     7081 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     7141 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     7201 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     7261 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     7321 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     7381 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     7441 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     7501 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     7561 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     7621 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     7681 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     7741 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     7801 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
     7861 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     7921 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta ccgcatcagg
     7981 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     8041 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     8101 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     8161 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     8221 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     8281 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     8341 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     8401 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgc
//
