LOCUS       Exported                8679 bp ds-DNA     circular SYN 06-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 5, 2023 from SnapGene 4.0.8
            http://www.snapgene.com
COMMENT     Notes o The plasmid has been transformed in SURE2 cells 
            (Stratagene), to avoid recombination. o The cassette 
            [5'TR-MCS-pA-3'TR] has been cloned between the SacII and KpnI sites 
            of the vector pBluescript II SK+. It can be removed by cutting 
            SacII-KpnI or by cutting with AscI. o The 5'TR contains the two 
            point mutations described in Lacoste et al., 2009 (Cell Stem Cell). 
            o The following primers can be used to sequence the plasmid: M13-rev
            and T3 (upstream of the 5'TR, top strand); M13-fw and T7 (downstream
            of the 3'TR, bottom strand). o Any restriction site can be used to 
            clone your favorite promoter/gene. However, I would suggest cloning 
            the promoter between XhoI-BamHI and the gene between BamHI-NotI, for
            consistency with previous constructs by Arnaud and others in the 
            lab. o You don't need to add a polyAdenylation (pA) sequence, since 
            it is already present in the vector. If undesired, the pA can be 
            removed with the EcoRI site.
FEATURES             Location/Qualifiers
     source          1..8679
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          326..557
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          2710..2772
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          4873..5103
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     repeat_region   1..325
                     /label=5' PB Terminal Repeat (ePB)
                     /note="5' Piggybac Transposon Terminal Repeat. Optimized 
                     for human IPSC by Arnaud et al. 2009."
     misc_feature    14..26
                     /label=13 bp terminal repeat (TR)
     misc_feature    27..29
                     /label=3 bp spacer
     misc_feature    30..48
                     /label=19 bp Internal Repeat (IR)
     enhancer        326..557
                     /label=core Insulator
                     /label=core insul enhancer
                     /label=core insul
     misc_feature    564..590
                     /label=I-CeuI site
                     /label=ICeuI site
                     /note="ICeuI site"
     misc_feature    591..605
                     /label=AarI site
     promoter        612..861
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA. 
                     Taken from Feng Zhang lab CRISPR sgRNA plasmids (Addgene 
                     42230, 60958, 61591)."
     primer_bind     612..632
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     783..802
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     regulatory      832..837
                     /regulatory_class="TATA_box"
     misc_signal     861
                     /label=TSS +1
                     /note="Transcription start site for human U6 promoter. 
                     First base of transcript. G is preferred."
     regulatory      881..886
                     /regulatory_class="terminator"
     misc_feature    887..901
                     /label=AarI site
     misc_feature    894..911
                     /label=ISceI site
                     /note="ISceI site"
     misc_feature    912..1455
                     /label=EF1 promoter
                     /note="/ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=green"
     promoter        942..1153
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1166..1434
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      1468..1477
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1474..2709
                     /codon_start=1
                     /label=EPN-1
                     /translation="MGARASGSKSGSGSDSGSKIEELFKKHKIVAVLRANSVEEAKKKA
                     LAVFLGGVHLIEITFTVPDADTVIKELSFLKEMGAIIGAGTVTSVEQCRKAVESGAEFI
                     VSPHLDEEISQFCKEKGVFYMPGVMTPTELVKAMKLGHTILKLFPGEVVGPQFVKAMKG
                     PFPNVKFVPTGGVNLDNVCEWFKAGVLAVGVGSALVKGTPVEVAEKAKAFVEKIRGCTE
                     QKLISEEDLASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVTCSVRQ
                     SSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELIVKAMQ
                     GLLKDGNPIPSAIAANSGIYLQSRPEPTAPPEESFRSGVETTTPPQKQEPIDKELYPLT
                     SLRSLFGNDPSSQ"
     CDS             1477..1491
                     /product="HIV-1 Gag_2-6 (Residues 2-6) Membrane binding 
                     (M)"
                     /label=Gag2-6
     CDS             1492..1527
                     /label=Linker
     CDS             1528..2136
                     /product="I3-01 Interaction domain "
                     /label=I3-01
     CDS             1777..1779
                     /label=Recoded to remove EcoRI
     CDS             2137..2166
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
     CDS             2167..2553
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
     CDS             2554..2709
                     /product="Plays a role in budding of the assembled particle
                     by interacting with the host class E VPS proteins TSG101 
                     and PDCD6IP/AIP1."
                     /label=HIV-1 p6
     CDS             2572..2583
                     /product="Late-budding domains (L domains) are short 
                     sequence motifs essential for viral particle budding. They 
                     recruit proteins of the host ESCRT machinery (Endosomal 
                     Sorting Complex Required for Transport) or ESCRT-associated
                     proteins. p6-gag contains two L domains: a PTAP/PSAP motif,
                     which interacts with the UEV domain of TSG101 and a 
                     LYPX(n)L motif which interacts with PDCD6IP/AIP1 
                     (https://www.uniprot.org/uniprot/P04591). Labeled 
                     ""ESCRT-I"" in Votteler et al. 2016, Extended Data Figure 
                     1a."
                     /label=HIV-1 p6-gag L domain
     CDS             2656..2685
                     /product="Late-budding domains (L domains) are short 
                     sequence motifs essential for viral particle budding. They 
                     recruit proteins of the host ESCRT machinery (Endosomal 
                     Sorting Complex Required for Transport) or ESCRT-associated
                     proteins. p6-gag contains two L domains: a PTAP/PSAP motif,
                     which interacts with the UEV domain of TSG101 and a 
                     LYPX(n)L motif which interacts with PDCD6IP/AIP1 
                     (https://www.uniprot.org/uniprot/P04591). Labeled ""ALIX"" 
                     in Votteler et al. 2016, Extended Data Figure 1a."
                     /label=HIV-1 p6-gag L domain
     CDS             2710..2772
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="GSGEGRGSLLTCGDVEENPGP"
     CDS             2773..3489
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     polyA_signal    3504..3740
                     /label=pA
                     /note="pA"
     polyA_signal    3617..3738
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3749..4198
                     /label=PGK promoter
                     /label=PGK(-)
                     /note="/ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff8040"
     regulatory      4208..4217
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4214..4612
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BlastR
                     /note="BSD
                     confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4630..4854
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        complement(4873..5103)
                     /label=core Insulator
                     /label=core insul(1) enhancer
                     /label=core insul(1)
     repeat_region   5104..5356
                     /label=3' PB Terminal Repeat (ePB)
                     /note="3' Piggybac Transposon Terminal Repeat. Optimized 
                     for human IPSC by Arnaud et al. 2009."
     misc_feature    5281..5299
                     /label=19 bp Internal Repeat (IR)
     misc_feature    5300..5330
                     /label=31 bp spacer
     misc_feature    5331..5343
                     /label=13 bp terminal repeat (TR)
     primer_bind     complement(5842..5858)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5866..5882
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5890..5920)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5935..5956
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6244..6832)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7003..7797)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(7798..7902)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7928..8383)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     8525..8541
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcagctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
       61 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      121 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      181 ataacaaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      241 actcatacga taattatatt gttatttcgt gttctactta cgtgataact tattatatat
      301 atattttctt gttatagata tccttgaggg acagcccccc cccaaagccc ccagggatgt
      361 aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc
      421 ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg
      481 tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg
      541 gggggatacg gggaaaaatc gattaactat aacggtccta aggtagcgaa cacctgcgct
      601 cgagcgacgt cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
      661 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
      721 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      781 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      841 tgtggaaagg acgaaacacc gaccaggtgg gcccacgcgt ttttttcacc tgcattaccc
      901 tgttatccct aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc
      961 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg
     1021 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     1081 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     1141 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc
     1201 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc
     1261 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg
     1321 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct
     1381 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct
     1441 gtgaccggcg cctacgctag cggatccgcc accatgggtg cgcgtgcgtc tggttctaaa
     1501 tctggctccg gctctgactc tggtagcaaa atcgaagagc tgttcaagaa acacaagatc
     1561 gttgccgtgc tgcgtgccaa tagtgtggaa gaagcgaaaa agaaagcgct ggcggttttc
     1621 ctgggcggcg ttcatctgat tgaaattacc tttaccgtgc cggatgcgga taccgtgatt
     1681 aaggaactga gctttctgaa ggaaatgggc gcgattattg gtgcgggcac cgtgaccagc
     1741 gtggagcagt gccgtaaagc ggtggaaagt ggcgccgagt tcattgtgag tccgcacctg
     1801 gacgaggaaa ttagccaatt ttgcaaggag aagggtgtgt tctatatgcc aggcgttatg
     1861 accccgaccg aactggtgaa agccatgaaa ctgggccata ccatcttaaa actgtttccg
     1921 ggtgaggtgg tgggtccgca gtttgttaaa gcgatgaaag gtccgtttcc gaatgtgaaa
     1981 tttgtgccaa ccggcggtgt taatctggac aatgtgtgcg aatggttcaa agcgggcgtg
     2041 ctggccgtgg gcgtgggcag cgcgttagtg aaaggcaccc cggtggaagt ggcggaaaag
     2101 gccaaggcgt tcgttgagaa gattcgtggc tgcaccgaac agaaactgat tagcgaagaa
     2161 gatctggctt caaactttac tcagttcgtg ctcgtggaca atggtgggac aggggatgtg
     2221 acagtggctc cttctaattt cgctaatggg gtggcagagt ggatcagctc caactcacgg
     2281 agccaggcct acaaggtgac atgcagcgtc aggcagtcta gtgcccagaa gagaaagtat
     2341 accatcaagg tggaggtccc caaagtggct acccagacag tgggcggagt cgaactgcct
     2401 gtcgccgctt ggaggtccta cctgaacatg gagctcacta tcccaatttt cgctaccaat
     2461 tctgactgtg aactcatcgt gaaggcaatg caggggctcc tcaaagacgg taatcctatc
     2521 ccttccgcca tcgccgctaa ctcaggtatc tacctgcagt ctcgtccgga accgaccgca
     2581 ccgccggaag aatctttccg ttctggtgtt gaaaccacca cgccaccgca aaaacaggag
     2641 ccgatcgaca aagaactgta cccgctgacc tctctgcgtt ctctgttcgg taacgacccg
     2701 tcttctcagg gatcgggaga gggcagagga agtctgctaa catgcggtga cgtcgaggag
     2761 aatcctggcc caatgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2821 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2881 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2941 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     3001 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     3061 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     3121 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     3181 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     3241 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     3301 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     3361 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     3421 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctcacggcat ggacgagctg
     3481 tacaagtaag cggccgcgtc gacgactcta gatcataatc agccatacca catttgtaga
     3541 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     3601 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     3661 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     3721 actcatcaat gtatcttaag cgccggcgct acacaagtgg cctctggcct cgcacacatt
     3781 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     3841 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     3901 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     3961 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     4021 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     4081 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     4141 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     4201 gggtaccgcc accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
     4261 aacggctaca atcaacagca tccccatctc tgaagattac agcgtcgcca gcgcagctct
     4321 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
     4381 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
     4441 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
     4501 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
     4561 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct aaggtcaccc
     4621 gcgcgcggac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     4681 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     4741 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     4801 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggttcgaa
     4861 ccagtacgat ggttttcccc gtatcccccc aggtgtctgc aggctcaaag agcagcgaga
     4921 agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt ccccgcacgc
     4981 tgccggctcg gggatgcggg gggagcgccg gaccggagcg gagccccggg cggctcgctg
     5041 ctgcccccta gcgggggagg gacgtaatta catccctggg ggctttgggg gggggctgtc
     5101 cctgataaaa gttttgttac tttatagaag aaattttgag tttttgtttt tttttaataa
     5161 ataaataaac ataaataaat tgtttgttga atttattatt agtatgtaag tgtaaatata
     5221 ataaaactta atatctattc aaattaataa ataaacctcg atatacagac cgataaaaca
     5281 catgcgtcaa ttttacgcat gattatcttt aacgtacgtc acaatatgat tatctttcta
     5341 gggttaatct agctgcggcg cgccatcagg atcatatcgt cgggtctttt ttccggctca
     5401 gtcatcgccc aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc
     5461 gcgaggttga agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg
     5521 agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg
     5581 gtgaactgtt cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt
     5641 tccggatggt cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact
     5701 gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg
     5761 acgggtatcc tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg
     5821 ggcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     5881 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     5941 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6001 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6061 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     6121 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     6181 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     6241 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     6301 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     6361 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     6421 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     6481 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     6541 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     6601 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     6661 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     6721 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     6781 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     6841 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     6901 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     6961 taaatcaatc taaagtatat atgagtaaac ttggtctgac agtcagaaga actcgtcaag
     7021 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     7081 gcggtcagcc cattcgccgc caagctcttc agcaatatca cgggtagcca acgctatgtc
     7141 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     7201 ttccaccatg atattcggca agcaggcatc gccatgggtc acgacgagat cctcgccgtc
     7261 gggcatgctc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgctcttc
     7321 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     7381 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     7441 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     7501 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     7561 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcttgcag
     7621 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     7681 cagccggaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     7741 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatcatact
     7801 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     7861 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     7921 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     7981 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     8041 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     8101 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     8161 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     8221 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     8281 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     8341 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     8401 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     8461 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     8521 cgttgtaaaa cgacggccag tgagcgcgcc tcgttcattc acgtttttga acccgtggag
     8581 gacgggcaga ctcgcggtgc aaatgtgttt tacagcgtga tggagcagat gaagatgctc
     8641 gacacgctgc agaacacgag ctccaccgcg gggcgcgcc
//
