LOCUS       Exported                8676 bp ds-DNA     circular SYN 06-JUN-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8676)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 5, 2023 from SnapGene 4.0.8
            http://www.snapgene.com
COMMENT     Notes o The plasmid has been transformed in SURE2 cells 
            (Stratagene), to avoid recombination. o The cassette 
            [5'TR-MCS-pA-3'TR] has been cloned between the SacII and KpnI sites 
            of the vector pBluescript II SK+. It can be removed by cutting 
            SacII-KpnI or by cutting with AscI. o The 5'TR contains the two 
            point mutations described in Lacoste et al., 2009 (Cell Stem Cell). 
            o The following primers can be used to sequence the plasmid: M13-rev
            and T3 (upstream of the 5'TR, top strand); M13-fw and T7 (downstream
            of the 3'TR, bottom strand). o Any restriction site can be used to 
            clone your favorite promoter/gene. However, I would suggest cloning 
            the promoter between XhoI-BamHI and the gene between BamHI-NotI, for
            consistency with previous constructs by Arnaud and others in the 
            lab. o You don't need to add a polyAdenylation (pA) sequence, since 
            it is already present in the vector. If undesired, the pA can be 
            removed with the EcoRI site.
FEATURES             Location/Qualifiers
     source          1..8676
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          326..557
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          2707..2769
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     source          4870..5100
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     repeat_region   1..325
                     /label=5' PB Terminal Repeat (ePB)
                     /note="5' Piggybac Transposon Terminal Repeat. Optimized 
                     for human IPSC by Arnaud et al. 2009."
     misc_feature    14..26
                     /label=13 bp terminal repeat (TR)
     misc_feature    27..29
                     /label=3 bp spacer
     misc_feature    30..48
                     /label=19 bp Internal Repeat (IR)
     enhancer        326..557
                     /label=core Insulator
                     /label=core insul enhancer
                     /label=core insul
     misc_feature    564..590
                     /label=I-CeuI site
                     /label=ICeuI site
                     /note="ICeuI site"
     misc_feature    591..605
                     /label=AarI site
     promoter        612..861
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA. 
                     Taken from Feng Zhang lab CRISPR sgRNA plasmids (Addgene 
                     42230, 60958, 61591)."
     primer_bind     612..632
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     783..802
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     regulatory      832..837
                     /regulatory_class="TATA_box"
     misc_signal     861
                     /label=TSS +1
                     /note="Transcription start site for human U6 promoter. 
                     First base of transcript. G is preferred."
     regulatory      881..886
                     /regulatory_class="terminator"
     misc_feature    887..901
                     /label=AarI site
     misc_feature    894..911
                     /label=ISceI site
                     /note="ISceI site"
     misc_feature    912..1455
                     /label=EF1 promoter
                     /note="/ApEinfo_fwdcolor=#ff80c0
                     /ApEinfo_revcolor=green"
     promoter        942..1153
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             1166..1434
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      1468..1477
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1474..2706
                     /codon_start=1
                     /label=EPN-1
                     /translation="MGARASGSKSGSGSDSGSKIEELFKKHKIVAVLRANSVEEAKKKA
                     LAVFLGGVHLIEITFTVPDADTVIKELSFLKEMGAIIGAGTVTSVEQCRKAVESGAEFI
                     VSPHLDEEISQFCKEKGVFYMPGVMTPTELVKAMKLGHTILKLFPGEVVGPQFVKAMKG
                     PFPNVKFVPTGGVNLDNVCEWFKAGVLAVGVGSALVKGTPVEVAEKAKAFVEKIRGCTA
                     SNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVTCSVRQSSAQKRKYTI
                     KVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELIVKAMQGLLKDGNPIP
                     SAIAANSGIYEQKLISEEDLQSRPEPTAPPEESFRSGVETTTPPQKQEPIDKELYPLTS
                     LRSLFGNDPSSQ"
     CDS             1477..1491
                     /product="HIV-1 Gag_2-6 (Residues 2-6) Membrane binding 
                     (M)"
                     /label=Gag2-6
     CDS             1492..1527
                     /label=Linker
     CDS             1528..2136
                     /product="I3-01 Interaction domain "
                     /label=I3-01
     CDS             1777..1779
                     /label=Recoded to remove EcoRI
     CDS             2137..2523
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
     CDS             2524..2553
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
     CDS             2551..2706
                     /product="Plays a role in budding of the assembled particle
                     by interacting with the host class E VPS proteins TSG101 
                     and PDCD6IP/AIP1."
                     /label=HIV-1 p6
     CDS             2569..2580
                     /product="Late-budding domains (L domains) are short 
                     sequence motifs essential for viral particle budding. They 
                     recruit proteins of the host ESCRT machinery (Endosomal 
                     Sorting Complex Required for Transport) or ESCRT-associated
                     proteins. p6-gag contains two L domains: a PTAP/PSAP motif,
                     which interacts with the UEV domain of TSG101 and a 
                     LYPX(n)L motif which interacts with PDCD6IP/AIP1 
                     (https://www.uniprot.org/uniprot/P04591). Labeled 
                     ""ESCRT-I"" in Votteler et al. 2016, Extended Data Figure 
                     1a."
                     /label=HIV-1 p6-gag L domain
     CDS             2653..2682
                     /product="Late-budding domains (L domains) are short 
                     sequence motifs essential for viral particle budding. They 
                     recruit proteins of the host ESCRT machinery (Endosomal 
                     Sorting Complex Required for Transport) or ESCRT-associated
                     proteins. p6-gag contains two L domains: a PTAP/PSAP motif,
                     which interacts with the UEV domain of TSG101 and a 
                     LYPX(n)L motif which interacts with PDCD6IP/AIP1 
                     (https://www.uniprot.org/uniprot/P04591). Labeled ""ALIX"" 
                     in Votteler et al. 2016, Extended Data Figure 1a."
                     /label=HIV-1 p6-gag L domain
     CDS             2707..2769
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="GSGEGRGSLLTCGDVEENPGP"
     CDS             2770..3486
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     polyA_signal    3501..3737
                     /label=pA
                     /note="pA"
     polyA_signal    3614..3735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    3746..4195
                     /label=PGK promoter
                     /label=PGK(-)
                     /note="/ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_graphicformat=arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0
                     /ApEinfo_revcolor=#ff8040"
     regulatory      4205..4214
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4211..4609
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BlastR
                     /note="BSD
                     confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    4627..4851
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     enhancer        complement(4870..5100)
                     /label=core Insulator
                     /label=core insul(1) enhancer
                     /label=core insul(1)
     repeat_region   5101..5353
                     /label=3' PB Terminal Repeat (ePB)
                     /note="3' Piggybac Transposon Terminal Repeat. Optimized 
                     for human IPSC by Arnaud et al. 2009."
     misc_feature    5278..5296
                     /label=19 bp Internal Repeat (IR)
     misc_feature    5297..5327
                     /label=31 bp spacer
     misc_feature    5328..5340
                     /label=13 bp terminal repeat (TR)
     primer_bind     complement(5839..5855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5863..5879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5887..5917)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5932..5953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6241..6829)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7000..7794)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(7795..7899)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7925..8380)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     8522..8538
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcagctagat taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat
       61 attgctctct ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg
      121 ccgcttggag ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact
      181 ataacaaccg cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta
      241 actcatacga taattatatt gttatttcgt gttctactta cgtgataact tattatatat
      301 atattttctt gttatagata tccttgaggg acagcccccc cccaaagccc ccagggatgt
      361 aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc gctccggtcc
      421 ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc acggggaagg
      481 tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg cagacacctg
      541 gggggatacg gggaaaaatc gattaactat aacggtccta aggtagcgaa cacctgcgct
      601 cgagcgacgt cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
      661 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
      721 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
      781 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
      841 tgtggaaagg acgaaacacc gaccaggtgg gcccacgcgt ttttttcacc tgcattaccc
      901 tgttatccct aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc
      961 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg
     1021 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     1081 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     1141 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc
     1201 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc
     1261 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg
     1321 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct
     1381 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct
     1441 gtgaccggcg cctacgctag cggatccgcc accatgggtg cgcgtgcgtc tggttctaaa
     1501 tctggctccg gctctgactc tggtagcaaa atcgaagagc tgttcaagaa acacaagatc
     1561 gttgccgtgc tgcgtgccaa tagtgtggaa gaagcgaaaa agaaagcgct ggcggttttc
     1621 ctgggcggcg ttcatctgat tgaaattacc tttaccgtgc cggatgcgga taccgtgatt
     1681 aaggaactga gctttctgaa ggaaatgggc gcgattattg gtgcgggcac cgtgaccagc
     1741 gtggagcagt gccgtaaagc ggtggaaagt ggcgccgagt tcattgtgag tccgcacctg
     1801 gacgaggaaa ttagccaatt ttgcaaggag aagggtgtgt tctatatgcc aggcgttatg
     1861 accccgaccg aactggtgaa agccatgaaa ctgggccata ccatcttaaa actgtttccg
     1921 ggtgaggtgg tgggtccgca gtttgttaaa gcgatgaaag gtccgtttcc gaatgtgaaa
     1981 tttgtgccaa ccggcggtgt taatctggac aatgtgtgcg aatggttcaa agcgggcgtg
     2041 ctggccgtgg gcgtgggcag cgcgttagtg aaaggcaccc cggtggaagt ggcggaaaag
     2101 gccaaggcgt tcgttgagaa gattcgtggc tgcaccgctt caaactttac tcagttcgtg
     2161 ctcgtggaca atggtgggac aggggatgtg acagtggctc cttctaattt cgctaatggg
     2221 gtggcagagt ggatcagctc caactcacgg agccaggcct acaaggtgac atgcagcgtc
     2281 aggcagtcta gtgcccagaa gagaaagtat accatcaagg tggaggtccc caaagtggct
     2341 acccagacag tgggcggagt cgaactgcct gtcgccgctt ggaggtccta cctgaacatg
     2401 gagctcacta tcccaatttt cgctaccaat tctgactgtg aactcatcgt gaaggcaatg
     2461 caggggctcc tcaaagacgg taatcctatc ccttccgcca tcgccgctaa ctcaggtatc
     2521 tacgaacaga aactgattag cgaagaagat ctgcagtctc gtccggaacc gaccgcaccg
     2581 ccggaagaat ctttccgttc tggtgttgaa accaccacgc caccgcaaaa acaggagccg
     2641 atcgacaaag aactgtaccc gctgacctct ctgcgttctc tgttcggtaa cgacccgtct
     2701 tctcagggat cgggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat
     2761 cctggcccaa tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2821 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2881 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2941 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3001 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3061 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3121 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3181 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     3241 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     3301 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3361 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     3421 atggtcctgc tggagttcgt gaccgccgcc gggatcactc acggcatgga cgagctgtac
     3481 aagtaagcgg ccgcgtcgac gactctagat cataatcagc cataccacat ttgtagaggt
     3541 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     3601 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3661 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3721 catcaatgta tcttaagcgc cggcgctaca caagtggcct ctggcctcgc acacattcca
     3781 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     3841 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     3901 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     3961 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     4021 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     4081 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     4141 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatggg
     4201 taccgccacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac
     4261 ggctacaatc aacagcatcc ccatctctga agattacagc gtcgccagcg cagctctctc
     4321 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc
     4381 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt
     4441 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct
     4501 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc
     4561 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag gtcacccgcg
     4621 cgcggactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     4681 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     4741 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     4801 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gttcgaacca
     4861 gtacgatggt tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc
     4921 gttcagagga aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc
     4981 cggctcgggg atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg
     5041 ccccctagcg ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtccct
     5101 gataaaagtt ttgttacttt atagaagaaa ttttgagttt ttgttttttt ttaataaata
     5161 aataaacata aataaattgt ttgttgaatt tattattagt atgtaagtgt aaatataata
     5221 aaacttaata tctattcaaa ttaataaata aacctcgata tacagaccga taaaacacat
     5281 gcgtcaattt tacgcatgat tatctttaac gtacgtcaca atatgattat ctttctaggg
     5341 ttaatctagc tgcggcgcgc catcaggatc atatcgtcgg gtcttttttc cggctcagtc
     5401 atcgcccaag ctggcgctat ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg
     5461 aggttgaagc ggcatggaaa gagtttgccg aggatgactg ctgctgcatt gacgttgagc
     5521 gaaaacgcac gtttaccatg atgattcggg aaggtgtggc catgcacgcc tttaacggtg
     5581 aactgttcgt tcaggccacc tgggatacca gttcgtcgcg gcttttccgg acacagttcc
     5641 ggatggtcag cccgaagcgc atcagcaacc cgaacaatac cggcgacagc cggaactgcc
     5701 gtgccggtgt gcagattaat gacagcggtg cggcgctggg atattacgtc agcgaggacg
     5761 ggtatcctgg ctggatgccg cagaaatgga catggatacc ccgtgagtta cccggcgggc
     5821 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     5881 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     5941 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     6001 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6061 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6121 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6181 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6241 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6301 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6361 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6421 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6481 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     6541 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     6601 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     6661 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     6721 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     6781 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     6841 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     6901 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     6961 atcaatctaa agtatatatg agtaaacttg gtctgacagt cagaagaact cgtcaagaag
     7021 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     7081 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     7141 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     7201 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     7261 catgctcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     7321 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     7381 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     7441 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     7501 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     7561 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cttgcagttc
     7621 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     7681 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     7741 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatactctt
     7801 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     7861 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     7921 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     7981 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     8041 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     8101 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     8161 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     8221 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     8281 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     8341 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     8401 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     8461 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     8521 tgtaaaacga cggccagtga gcgcgcctcg ttcattcacg tttttgaacc cgtggaggac
     8581 gggcagactc gcggtgcaaa tgtgttttac agcgtgatgg agcagatgaa gatgctcgac
     8641 acgctgcaga acacgagctc caccgcgggg cgcgcc
//
