LOCUS       Exported               11053 bp ds-DNA     circular SYN 30-MRT-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    750 pLV_CMV_dnMCAK
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11053)
  AUTHORS   Petra Bakker
  TITLE     Direct Submission
  JOURNAL   Exported donderdag 13 jul 2023 from SnapGene 2.8.3
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11053
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             1..634
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_binding    636..653
                     /label=PBS
                     /note="PBS"
     misc_feature    681..806
                     /note="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /note="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2028..2143
                     /note="cPPT/CTS"
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2215..2594
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2595..2798
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2837..2846
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2843..3559
                     /codon_start=1
                     /product="enhanced GFP"
                     /note="EGFP"
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3605..5761
                     /codon_start=1
                     /note="dnMCAK"
                     /translation="MESLPARLFPGLSIKIQRSNGLIHSANISTVNVEKSCVSVEWIEG
                     GNTKGKEIDFDDVAAINPELLQLLPLHPKDNLPLQENVTVPKQKRRAVNSKIPAPKEGL
                     RARATRMATVPEVRIATQENEMEVELPVATNSRKQFSVATGLPRPSCPAMTELPLSMVS
                     EEAEEQVHPTRSTSSANPARRKACIVKEMEKMKNKREEKRAQNSEIRIKRAQEYDSSFP
                     NWEFARMIKEFRVTIECHPLTLTDPTEEHRICVCVRKRPLNKQELAKKEIDVISVPSKC
                     LLFVHEPKLKVDLTKYLENQAFCFDFAFDETASNEVVYRFTARPLVQTIFEGGKATCFA
                     YGQTGSGKTHTMGGDLSGKSQNTSKGIYAMASRDVFLLKSQPRYRNLNLEVYVTFFEIY
                     NGKVFDLLNKKAKLRVLEDSKQQVQVVGLQEYLVNCADDVIKMLNMGSACRTSGQTFAN
                     SNSSRSHACFQILLRAKGRLHGKFSLVDLAGNERGADTSSADRQTRMEGAEINKSLLAL
                     KECIRALGQNKAATPFAESALTQVLRDSFIGENSRTCMIAMISPGISSCEYTLNTLRYA
                     DRVKELSPHSGLSGEQPIQMETEEMEASSNGTSLAVNFKEEEELSSQMSSFNEAMSQIR
                     ELEERAMEELREIIQQGPGWLELSEMTDQPDYDLETFVNKAESALTQQTKHFSALREVI
                     KALRVAMQLEEQASKQMNSKKRHQ"
     promoter        5839..6338
                     /note="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             6359..6958
                     /codon_start=1
                     /label=Puro(R)
                     /note="Puro(R)"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6975..7563
                     /note="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(7446..7457)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     LTR             7770..8403
                     /note="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8531..8547)
                     /note="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8555..8571
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8579..8609)
                     /note="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8624..8645
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding site"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8933..9521)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9692..10552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10553..10657)
                     /gene="bla"
                     /note="AmpR promoter"
     polyA_signal    10705..10839
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagcg cgttgacatt
     2221 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
     2281 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
     2341 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
     2401 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
     2461 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
     2521 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
     2581 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
     2641 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
     2701 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
     2761 gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc
     2821 gctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
     2881 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     2941 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     3001 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     3061 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     3121 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     3181 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     3241 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     3301 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     3361 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     3421 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
     3481 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     3541 catggacgag ctgtacaagt ccggactcag atctcgagct caagcttcga attcgaggcg
     3601 cgccatggag tcgcttcccg ctcgattgtt tcctggtctc tccatcaaga ttcaacgcag
     3661 taatggctta attcacagtg ccaacataag cacagtgaac gtggagaaat cctgtgtttc
     3721 agtggaatgg atagaaggag gtaacacgaa aggcaaagag attgattttg atgatgtggc
     3781 tgcaataaac ccagaacttc tacagcttct tcccttacac ccaaaggaca atctgccact
     3841 gcaggaaaat gtaacagttc cgaagcaaaa acgcagagca gtcaactcca aaattccagc
     3901 tccaaaagaa ggtcttcgag cacgtgcaac tcgcatggca actgtcccag aggttcgaat
     3961 tgccactcag gagaatgaaa tggaggtgga gctgcctgtg gctacaaact ctcgaaagca
     4021 gttttcggtt gccactggcc tccctaggcc ctcctgtcct gcaatgacag aattaccgtt
     4081 gtcgatggtc agcgaggagg cagaagagca agtccatccc acccgaagca catcttctgc
     4141 aaaccctgct cggaggaaag catgtattgt gaaggaaatg gaaaaaatga agaacaagcg
     4201 ggaagagaag agggcccaga actcggaaat aagaataaag cgagctcagg aatatgacag
     4261 cagctttcca aactgggaat ttgcccggat gattaaagaa ttccgggtta ctatagaatg
     4321 tcacccactt actctgactg atcctacgga agagcacagg atctgcgtgt gtgtgaggaa
     4381 gcgcccactg aataagcaag aactggccaa gaaagaaatc gatgtgattt ctgttcccag
     4441 caagtgtttg ctctttgtgc atgaacccaa attgaaagtg gacttgacaa agtatctgga
     4501 gaatcaggca ttctgcttcg attttgcctt tgatgaaaca gcttcaaatg aagtcgtcta
     4561 caggttcaca gcaaggccac tggtacagac gatttttgaa gggggaaaag caacctgctt
     4621 tgcctatggg cagacgggca gtgggaagac tcacacaatg ggtggagacc tgtctggtaa
     4681 atctcagaat acatctaaag ggatctatgc aatggcttcc cgggatgtct tccttctgaa
     4741 gagtcagcct cgctaccgga acttaaactt ggaagtttat gtgacattct ttgagatcta
     4801 caatgggaag gtgtttgatc tgctcaacaa gaaggccaag ctacgtgtac tagaagacag
     4861 caagcagcag gtgcaggttg tgggacttca ggagtacctg gttaactgtg ctgatgatgt
     4921 catcaagatg ctcaacatgg gcagtgcctg caggacttct ggacagactt ttgccaactc
     4981 caattcttcc cgctcccatg cctgcttcca gattcttctt cgagccaaag ggagattgca
     5041 tggcaaattc tccttggtgg atctggcagg gaatgaacga ggggctgaca cttctagtgc
     5101 tgaccgccag actcgcatgg agggtgcaga gattaacaag tctctcctag cactgaagga
     5161 gtgcatcagg gcgctgggac agaacaaggc tgccacccca ttcgctgaga gcgcactgac
     5221 tcaggtgcta agggattcat tcattgggga gaactcgagg acttgcatga ttgccatgat
     5281 ctcaccaggc ataagctcct gtgaatatac tttaaacacg ctgagatatg cagacagagt
     5341 caaggagctg agcccccaca gcgggctcag tggagagcag ccaattcaaa tggaaacgga
     5401 agagatggaa gccagctcta atgggacctc gctggcagtc aatttcaagg aagaagagga
     5461 gctgtcttcc cagatgtcca gctttaatga agccatgagt cagatcaggg agctggaaga
     5521 gagggccatg gaggagctca gggagatcat acagcaagga ccaggctggc ttgagctctc
     5581 tgagatgact gatcagccag actatgactt agagaccttt gtgaacaaag cagaatctgc
     5641 cctgactcag cagactaagc acttctcagc cctgcgagaa gtcatcaagg ccctgcgtgt
     5701 ggccatgcaa ctggaagaac aggctagcaa acagatgaac agcaagaaac ggcaccaatg
     5761 attaattaac tcgacggtac cgcgggcccg ggatccgcgg ccgcgccggc tctagatcgc
     5821 gaacgcgtga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     5881 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     5941 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     6001 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     6061 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     6121 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     6181 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     6241 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     6301 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     6361 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     6421 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     6481 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     6541 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     6601 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     6661 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     6721 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     6781 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     6841 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     6901 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     6961 ggcgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7021 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7081 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7141 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7201 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7261 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7321 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     7381 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7441 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7501 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7561 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     7621 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     7681 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     7741 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
     7801 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     7861 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     7921 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     7981 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     8041 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     8101 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     8161 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     8221 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8281 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8341 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     8401 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     8461 cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg
     8521 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     8581 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     8641 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     8701 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     8761 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     8821 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     8881 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     8941 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     9001 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     9061 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     9121 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     9181 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     9241 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     9301 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     9361 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     9421 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     9481 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     9541 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     9601 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     9661 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     9721 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     9781 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     9841 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     9901 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9961 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
    10021 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
    10081 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
    10141 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
    10201 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
    10261 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
    10321 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
    10381 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
    10441 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
    10501 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
    10561 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
    10621 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
    10681 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
    10741 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    10801 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
    10861 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
    10921 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
    10981 taaatatgta ctacaaactt agtagttttt aaagaaattg tatttgttaa atatgtacta
    11041 caaacttagt agt
//
