LOCUS       pAAV_hSyn_WiChR_TS_mSca 6290 bp ds-DNA     circular     13-JUL-2023
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        1..467
                     /label="hSynapsin I Promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    230..235
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    299..304
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    430..449
                     /label="Synapsin-for"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    480..484
                     /label="BamH1"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    485..490
                     /label="Kozak"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    491..1423
                     /label="WlChR1"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             491..2221
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSACPTPPTVVAPTDQSPNPNGGTTSCAQAAPSPQMSMNYTLLATIDGFSAVILLGSGVVFWYYSWRGTGFGAHPHPKRRPLELNTLSAAIAFLTGLMYVAKTSDFARDSTSEFSLNRYWYSDYIVTCPLIVMDLCFTVDVPYKLAFTGSTAVLLVIALASFIVRSPEKYIYFAIGCTLFSIFFYFFFNEVRKRIRYVPDCAKKDLERAMFIFFGFWPMFPVLWLLGFHTTGVISEEAFFIIHAFLDITCKTIFGVFMLRCRLHIEDYAWDELKRQRQEIDEENQKTLTAAGADGATVMMPVEEDDPYASGRTAKSRITSEGEYIPLDQIDINVAVPMVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGGAVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKMALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHSTGGMDELYKFCYENEV*"
     misc_feature    complement(520..525)
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    620..625
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    749..751
                     /label="L87"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    761..763
                     /label="I91"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1208..1210
                     /label="F240"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1376..1381
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1424..1429
                     /label="BsiWI"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1433..1492
                     /label="TS"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1446..1466
                     /label="TS-Site_ for"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1502..2197
                     /label="mScarlet"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(1570..1591)
                     /label="mScarlet-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1655..1660)
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2198..2221
                     /label="ER-Site"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2198..2221
                     /label="ER"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2251..2838
                     /label="WPRE"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGTNGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKNPSMLYLATGHRVQWLRYAEWLLTCPVILIRLSNLTGLSNDYSRRTMGLLVSDIGTIVWGATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVSWGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDIRKTTKLNIGGTEIEVETLVEDEAEAGAVPAAATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"
     misc_feature    complement(2316..2341)
                     /label="miniWPRE-rev"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2501..2506
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2678..2683
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     terminator      2867..3345
                     /label="hGH_PA_terminator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRIDTERCSRDLRVASL*"
     polyA_signal    2868..3348
                     /label="hGH polyA signal"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    complement(2870..2875)
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2973..2992)
                     /label="CMV5-R"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     repeat_region   3385..3525
                     /label="ITR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(3385..3525)
                     /label="5' ITR"
                     /ApEinfo_revcolor="#d84e4e"
                     /ApEinfo_fwdcolor="#d84e4e"
     misc_feature    complement(3385..3525)
                     /label="3' ITR"
                     /ApEinfo_revcolor="#5566f5"
                     /ApEinfo_fwdcolor="#5566f5"
     rep_origin      3600..4055
                     /label="M13 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      3617..3923
                     /label="f1_origin"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRIDTERCSRDLRVASL*"
     rep_origin      3617..3923
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    4191..4213
                     /label="pGEX3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    4191..4213
                     /label="pGEX_3_primer"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRIDTERCSRDLRVASL*"
     misc_feature    4195..4213
                     /label="GEX-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4302..4321)
                     /label="pBR-F"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(4313..4318)
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(4334..4353)
                     /label="sCOS1F"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        4372..4400
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRIDTERCSRDLRVASL*"
     gene            4442..5302
                     /label="Ampicillin"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /gene="Ampicillin"
                     /translation="MLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCALCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCRGNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGSPFGPPPRIDTERCSRDLRVASL*"
     CDS             4640..5299
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      5397..6079
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      5457..6076
                     /label="pBR322_origin"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     misc_feature    5643..5648
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6000..6005
                     /label="Kozak"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    6075..6094
                     /label="ASK-FN2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     repeat_region   6123..6263
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    6123..6263
                     /label="5' ITR"
                     /ApEinfo_revcolor="#d84e4e"
                     /ApEinfo_fwdcolor="#d84e4e"
     misc_feature    6123..6263
                     /label="3' ITR"
                     /ApEinfo_revcolor="#5566f5"
                     /ApEinfo_fwdcolor="#5566f5"
     promoter        6287..465
                     /label="SYN1"
                     /ApEinfo_revcolor="#1f36a9"
                     /ApEinfo_fwdcolor="#1f36a9"
ORIGIN
        1 agagggccct gcgtatgagt gcaagtgggt tttaggacca ggatgaggcg gggtgggggt
       61 gcctacctga cgaccgaccc cgacccactg gacaagcacc caacccccat tccccaaatt
      121 gcgcatcccc tatcagagag ggggagggga aacaggatgc ggcgaggcgc gtgcgcactg
      181 ccagcttcag caccgcggac agtgccttcg cccccgcctg gcggcgcgcg ccaccgccgc
      241 ctcagcactg aaggcgcgct gacgtcactc gccggtcccc cgcaaactcc ccttcccggc
      301 caccttggtc gcgtccgcgc cgccgccggc ccagccggac cgcaccacgc gaggcgcgag
      361 ataggggggc acgggcgcga ccatctgcgc tgcggcgccg gcgactcagc gctgcctcag
      421 tctgcggtgg gcagcggagg agtcgtgtcg tgcctgagag cgcagtcgag aaggtaccgg
      481 atccgccacc atgagcgcct gccccacccc ccccaccgtg gtggccccca ccgaccagag
      541 ccccaacccc aacggcggca ccaccagctg cgcccaggcc gcccccagcc cccagatgag
      601 catgaactac accctgctgg ccaccatcga cggcttcagc gccgtgatcc tgctgggcag
      661 cggcgtggtg ttctggtact acagctggcg cggcaccggc ttcggcgccc acccccaccc
      721 caagcgccgc cccctggagc tgaacaccct gagcgccgcc atcgccttcc tgaccggcct
      781 gatgtacgtg gccaagacca gcgacttcgc ccgcgacagc accagcgagt tcagcctgaa
      841 ccgctactgg tacagcgact acatcgtgac ctgccccctg atcgtgatgg acctgtgctt
      901 caccgtggac gtgccctaca agctggcctt caccggcagc accgccgtgc tgctggtgat
      961 cgccctggcc agcttcatcg tgcgcagccc cgagaagtac atctacttcg ccatcggctg
     1021 caccctgttc agcatcttct tctacttctt cttcaacgag gtgcgcaagc gcatccgcta
     1081 cgtgcccgac tgcgccaaga aggacctgga gcgcgccatg ttcatcttct tcggcttctg
     1141 gcccatgttc cccgtgctgt ggctgctggg cttccacacc accggcgtga tcagcgagga
     1201 ggccttcttc atcatccacg ccttcctgga catcacctgc aagaccatct tcggcgtgtt
     1261 catgctgcgc tgccgcctgc acatcgagga ctacgcctgg gacgagctga agcgccagcg
     1321 ccaggagatc gacgaggaga accagaagac cctgaccgcc gccggcgccg acggcgccac
     1381 cgtgatgatg cccgtggagg aggacgaccc ctacgccagc ggacgtacgG CGAAGAGCAG
     1441 GATCACCAGC GAGGGCGAGT ACATCCCCCT GGACCAGATC GACATCAACG TGGCGGTACC
     1501 CATGGTGAGC AAGGGCGAGG CAGTGATCAA GGAGTTCATG CGGTTCAAGG TGCACATGGA
     1561 GGGCTCCATG AACGGCCACG AGTTCGAGAT CGAGGGCGAG GGCGAGGGCC GCCCCTACGA
     1621 GGGCACCCAG ACCGCCAAGC TGAAGGTGAC CAAGGGTGGC CCCCTGCCCT TCTCCTGGGA
     1681 CATCCTGTCC CCTCAGTTCA TGTACGGCTC CAGGGCCTTC ACCAAGCACC CCGCCGACAT
     1741 CCCCGACTAC TATAAGCAGT CCTTCCCCGA GGGCTTCAAG TGGGAGCGCG TGATGAACTT
     1801 CGAGGACGGC GGCGCCGTGA CCGTGACCCA GGACACCTCC CTGGAGGACG GCACCCTGAT
     1861 CTACAAGGTG AAGCTCCGCG GCACCAACTT CCCTCCTGAC GGCCCCGTAA TGCAGAAGAA
     1921 GACAATGGGC TGGGAAGCGT CCACCGAGCG GTTGTATCCC GAGGACGGCG TGCTGAAGGG
     1981 CGACATTAAG ATGGCCCTGC GCCTGAAGGA CGGCGGCCGC TACCTGGCGG ACTTCAAGAC
     2041 CACCTACAAG GCCAAGAAGC CCGTGCAGAT GCCCGGCGCC TACAACGTCG ACCGCAAGTT
     2101 GGACATCACC TCCCACAACG AGGACTACAC CGTGGTGGAA CAGTACGAAC GCTCCGAGGG
     2161 CCGCCACTCC ACCGGCGGCA TGGACGAGCT GTACAAGTTC TGCTACGAGA ACGAGGTGTA
     2221 ATGAGAATTC GATATCAagc ttatcgataa tcaacctctg gattacaaaa tttgtgaaag
     2281 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2341 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2401 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2461 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2521 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     2581 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     2641 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     2701 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     2761 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     2821 ttgggccgcc tccccgcatc gataccgagc gctgctcgag agatctacgg gtggcatccc
     2881 tgtgacccct ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc
     2941 cttgtcctaa taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt
     3001 atggggtgga ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc
     3061 tgcggggtct attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc
     3121 tccgcctcct gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag
     3181 gcatgcatga ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata
     3241 ttggccaggc tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa
     3301 ttgctgggat tacaggcgtg aaccactgct cccttccctg tccttctgat tttgtaggta
     3361 accacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
     3421 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     3481 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
     3541 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
     3601 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3661 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3721 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3781 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
     3841 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3901 actcttgttc caaactggaa caacactcaa ccctatctcg ggctattctt ttgatttata
     3961 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4021 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
     4081 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4141 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4201 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4261 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     4321 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4381 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4441 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4501 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4561 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4621 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4681 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4741 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4801 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4861 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4921 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4981 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     5041 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     5101 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     5161 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     5221 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     5281 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     5341 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5401 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5461 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5521 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5581 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     5641 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5701 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5761 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5821 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5881 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5941 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     6001 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     6061 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     6121 gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg
     6181 tcgggcgacc tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc
     6241 caactccatc actaggggtt cctgcggccg cacgcgtgtg tctagactgc
//