LOCUS       hACVR1_R206H_TAS        1697 bp DNA     linear   UNA 27-JUL-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Allison Marvin
  TITLE     Direct Submission
  JOURNAL   Exported Jul 27, 2023 from SnapGene 6.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        55..73
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             105..1631
                     /codon_start=1
                     /label=ACVR1
                     /note="color: #a6acb3"
                     /translation="MVDGVMILPVLIMIALPSPSMEDEKPKVNPKLYMCVCEGLSCGNE
                     DHCEGQQCFSSLSINDGFHVYQKGCFQVYEQGKMTCKTPPSPGQAVECCQGDWCNRNIT
                     AQLPTKGKSFPGTQNFHLEVGLIILSVVFAVCLLACLLGVALRKFKXRNQERLNPRDVE
                     YGTIEGLITTNVGDSTLADLLDHSCTAGSGAGLPFLVQRTVAHQITLLECVGKGRYGEV
                     WRGSWQGENVAVKIFSSRDEKSWFRETELYNTVMLRHENILGFIASDMTSRHSSTQLWL
                     ITHYHEMGSLYDYLQLTTLDTVSCLRIVLSIASGLAHLHIEIFGTQGKPAIAHRDLKSK
                     NILVKKNGQCCIADLGLAVMHSQSTNQLDVGNNPRVGTKRYMAPEVLDETIQVDCFDSY
                     KRVDIWAFGLVLWEVARRMVSNGIVEDYKPPFYDVVPNDPSFEDMRKVVCVDQQRPNIP
                     NRWFSDPTLTSLAKLMKECWYQNPSARLTALRIKKTLTKIDNSLDKLKTDC"
     misc_feature    672..674
                     /label=S190A
                     /note="color: #a6acb3"
     misc_feature    684..686
                     /label=S194A
                     /note="color: #a6acb3"
     misc_feature    720..722
                     /label=R206H
                     /note="color: #a6acb3"
     CDS             1650..1697
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDKDYKDDDDK"
ORIGIN
        1 agcatgctcg tttagggaac cgccattctg cctgggaacg tcggagcaag cttgatttag
       61 gtgacactat agaatacaag ctacttgttc tttttgcagg atccatggta gatggagtga
      121 tgattcttcc tgtgcttatc atgattgctc tcccctcccc tagtatggaa gatgagaagc
      181 ccaaggtcaa ccccaaactc tacatgtgtg tgtgtgaagg tctctcctgc ggtaatgagg
      241 accactgtga aggccagcag tgcttttcct cactgagcat caacgatggc ttccacgtct
      301 accagaaagg ctgcttccag gtttatgagc agggaaagat gacctgtaag accccgccgt
      361 cccctggcca agctgtggag tgctgccaag gggactggtg taacaggaac atcacggccc
      421 agctgcccac taaaggaaaa tccttccctg gaacacagaa tttccacttg gaggttggcc
      481 tcattattct ctctgtagtg ttcgcagtat gtcttttagc ctgcctgctg ggagttgctc
      541 tccgaaaatt taaarggcgc aaccaagaac gcctcaatcc ccgagacgtg gagtatggca
      601 ctatcgaagg gctcatcacc accaatgttg gagacagcac tttagcagat ttattggatc
      661 attcgtgtac agcaggaagt ggcgctggtc ttccttttct ggtacaaaga acagtggctc
      721 accagattac actgttggag tgtgtcggga aaggcaggta tggtgaggtg tggaggggca
      781 gctggcaagg ggaaaatgtt gccgtgaaga tcttctcctc ccgtgatgag aagtcatggt
      841 tcagggaaac ggaattgtac aacactgtga tgctgaggca tgaaaatatc ttaggtttca
      901 ttgcttcaga catgacatca agacactcca gtacccagct gtggttaatt acacattatc
      961 atgaaatggg atcgttgtac gactatcttc agcttactac tctggataca gttagctgcc
     1021 ttcgaatagt gctgtccata gctagtggtc ttgcacattt gcacatagag atatttggga
     1081 cccaagggaa accagccatt gcccatcgag atttaaagag caaaaatatt ctggttaaga
     1141 agaatggaca gtgttgcata gcagatttgg gcctggcagt catgcattcc cagagcacca
     1201 atcagcttga tgtggggaac aatccccgtg tgggcaccaa gcgctacatg gcccccgaag
     1261 ttctagatga aaccatccag gtggattgtt tcgattctta taaaagggtc gatatttggg
     1321 cctttggact tgttttgtgg gaagtggcca ggcggatggt gagcaatggt atagtggagg
     1381 attacaagcc accgttctac gatgtggttc ccaatgaccc aagttttgaa gatatgagga
     1441 aggtagtctg tgtggatcaa caaaggccaa acatacccaa cagatggttc tcagacccga
     1501 cattaacctc tctggccaag ctaatgaaag aatgctggta tcaaaatcca tccgcaagac
     1561 tcacagcact gcgtatcaaa aagactttga ccaaaattga taattccctc gacaaattga
     1621 aaactgactg taatcgattt aaagctatgg attacaagga tgatgatgat aaggattaca
     1681 aggatgatga tgataag
//
