LOCUS       pATetO_6XHis_T5_Exonuclease        3721 bp    DNA     circular UNA 03-NOV-2021
DEFINITION  tet-inducible system with N-term periplasmic tag and C-term strep
            tag.
ACCESSION   urn.local...h-e10uc7z
VERSION     urn.local...h-e10uc7z
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    complement(138..761)
                     /note="Geneious type: ORF"
                     /standard_name="tet repressor"
     misc_feature    complement(771..1631)
                     /note="Geneious type: ORF"
                     /standard_name="AmpR"
     regulatory      complement(1632..1715)
                     /note="Geneious type: promoter"
                     /standard_name="bla1"
     regulatory      1747..1837
                     /note="Geneious type: terminator"
                     /standard_name="rrnD T1 (Endy 2013)"
     regulatory      1881..1946
                     /note="Geneious type: promoter"
                     /standard_name="tet PA (Kostner 2006)"
     misc_feature    1970..1979
                     /standard_name="RBS"
     sig_peptide     <1991..2023
                     /standard_name="6X His tag wATG"
     primer_bind     complement(2018..2035)
                     /standard_name="Left flank"
     misc_feature    2024..2035
                     /note="Geneious type: polylinker"
                     /standard_name="Factor Xa site"
     gene            2036..2908
                     /standard_name="T5 Exonuclease"
     misc_feature    2039..2908
                     /note="Geneious type: Editing History Replacement"
                     /standard_name="CGTGGTATGCTGCCGCTGTTCGAACCGAAAGGTCGTGTTCTG
                     CTGGTTGACGGTCACCACCTGGCGTACCGTACCTTCCACGCGCTGAAAGGTCTGACCA
                     CCTCTCGTGGTGAACCGGTTCAGGCGGTTTACGGTTTCGCGAAATCTCTGCTGAAAGC
                     GCTGAAAGAAGACGGTGACGCGGTTATCGTTGTTTTCGACGCGAAAGCGCCGTCTTTC
                     CGTCACGAAGCGTACGGTGGTTACAAAGCGGGTCGTGCGCCGACCCCGGAAGACTTCC
                     CGCGTCAGCTGGCGCTGATCAAAGAACTGGTTGACCTGCTGGGTCTGGCGCGTCTGGA
                     AGTTCCGGGTTACGAAGCGGACGACGTTCTGGCGTCTCTGGCGAAAAAAGCGGAAAAA
                     GAAGGTTACGAAGTTCGTATCCTGACCGCGGACAAAGACCTGTACCAGCTGCTGTCTG
                     ACCGTATCCACGCGCTGCACCCGGAAGGTTACCTGATCACCCCGGCGTGGCTGTGGGA
                     AAAATACGGTCTGCGTCCGGACCAGTGGGCGGACTACCGTGCGCTGACCGGTGACGAA
                     TCTGACAACCTGCCGGGTGTTAAAGGTATCGGTGAAAAAACCGCGCGTAAACTGCTGG
                     AAGAATGGGGTTCTCTGGAAGCGCTGCTGAAAAACCTGGACCGTCTGAAACCGGCGAT
                     CCGTGAAAAAATCCTGGCGCACATGGACGACCTGAAACTGTCTTGGGACCTGGCGAAA
                     GTTCGTACCGACCTGCCGCTGGAAGTTGACTTCGCGAAACGTCGTGAACCGGACCGTG
                     AACGTCTGCGTGCGTTCCTGGAACGTCTGGAATTCGGTTCTCTGCTGCACGAATTCGG
                     TCTGCTGGAATCTCCGAAAGCGCTGGAAGAAGCGCCGTGGCCGCCGCCGGAAGGTGCG
                     TTCGTTGGTTTCGTTCTGTCTCGTAAAGAACCGATGTGGGCGGACCTGCTGGCGCTGG
                     CGGCGGCGCGTGGTGGTCGTGTTCACCGTGCGCCGGAACCGTACAAAGCGCTGCGTGA
                     CCTGAAAGAAGCGCGTGGTCTGCTGGCGAAAGACCTGTCTGTTCTGGCGCTGCGTGAA
                     GGTCTGGGTCTGCCGCCGGGTGACGACCCGATGCTGCTGGCGTACCTGCTGGACCCGT
                     CTAACACCACCCCGGAAGGTGTTGCGCGTCGTTACGGTGGTGAATGGACCGAAGAAGC
                     GGGTGAACGTGCGGCGCTGTCTGAACGTCTGTTCGCGAACCTGTGGGGTCGTCTGGAA
                     GGTGAAGAACGTCTGCTGTGGCTGTACCGTGAAGTTGAACGTCCGCTGTCTGCGGTTC
                     TGGCGCACATGGAAGCGACCGGTGTTCGTCTGGACGTTGCGTACCTGCGTGCGCTGTC
                     TCTGGAAGTTGCGGAAGAAATCGCGCGTCTGGAAGCGGAAGTTTTCCGTCTGGCGGGT
                     CACCCGTTCAACCTGAACTCTCGTGACCAACTGGAACGTGTTCTGTTCGACGAACTGG
                     GTCTGCCGGCGATCGGTAAAACCGAAAAAACCGGTAAACGTTCTACCTCTGCGGCGGT
                     TCTGGAAGCGCTGCGTGAAGCGCACCCGATCGTTGAAAAAATCCTGCAGTACCGTGAA
                     CTGACCAAACTGAAATCTACCTACATCGACCCGCTGCCGGACCTGATCCACCCGCGTA
                     CCGGTCGTCTGCACACCCGTTTCAACCAGACCGCGACCGCGACCGGTCGTCTGTCTTC
                     TTCTGACCCGAACCTGCAGAACATCCCGGTTCGTACCCCACTGGGTCAGCGTATCCGT
                     CGTGCGTTCATCGCGGAAGAAGGTTGGCTTCTGGTTGCGCTGGACTACTCTCAGATCG
                     AACTGCGTGTTCTGGCGCACCTGTCTGGTGACGAAAACCTGATCCGTGTTTTCCAGGA
                     AGGTCGTGACATCCACACCGAAACCGCGTCTTGGATGTTCGGTGTTCCGCGTGAAGCG
                     GTTGACCCGCTGATGCGTCGTGCGGCGAAAACCATCAACTTCGGTGTTCTGTACGGTA
                     TGTCTGCGCACCGTCTGTCTCAGGAACTGGCGATCCCGTACGAAGAAGCGCAGGCGTT
                     CATCGAACGTTACTTCCAGTCTTTCCCGAAAGTTCGTGCGTGGATCGAAAAAACCCTG
                     GAAGAAGGTCGTCGTCGTGGTTACGTTGAAACCCTGTTCGGTCGTCGTCGTTACGTTC
                     CGGACCTGGAAGCGCGTGTTAAATCTGTTCGTGAAGCGGCGGAACGTATGGCGTTCAA
                     CATGCCGGTTCAGGGTACCGCGGCGGACCTGATGAAACTGGCGATGGTTAAACTGTTC
                     CCGCGTCTGGAAGAAATGGGTGCGCGTATGCTGCTGCAGGTTCACGACGAACTGGTTC
                     TGGAAGCGCCGAAAGAACGTGCGGAAGCGGTTGCGCGTCTGGCGAAAGAAGTTATGGA
                     AGGTGTTTACCCGCTGGCGGTTCCGCTGGAAGTTGAAGTTGGTATCGGTGAAGACTGG
                     CTGTCTGCGAAAGAA"
     primer_bind     2909..2938
                     /standard_name="Right flank"
     regulatory      2955..3036
                     /note="Geneious type: terminator"
                     /standard_name="rnpB T1 (Endy 2013)"
     rep_origin      complement(3118..3705)
ORIGIN      
        1 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
       61 agattatcaa aaaggatctt cacctagatc cttttaaact agtgaagtta ccatcacgga
      121 aaaaggttat gctgctttta agacccactt tcacatttaa gttgtttttc taatccgcat
      181 atgatcaatt caaggccgaa taagaaggct ggctctgcac cttggtgatc aaataattcg
      241 atagcttgtc gtaataatgg cggcatacta tcagtagtag gtgtttccct ttcttcttta
      301 gcgacttgat gctcttgatc ttccaatacg caacctaaag taaaatgccc cactgcgctg
      361 agtgcatata atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc taattgattt
      421 tcgagagttt catactgttt ttctgtaggc cgtgtaccta aatgtacttt tgctccatcg
      481 cgatgactta gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa atcttgccag
      541 ctttcccctt ctaaagggca aaagtgagta tggtgcctat ctaacatctc aatggctaag
      601 gcgtcgagca aagcccgctt attttttaca tgccaataca atgtaggctg ctctacacct
      661 agcttctggg cgagtttacg ggttgttaaa ccttcgattc cgacctcatt aagcagctct
      721 aatgcgctgt taatcacttt acttttatct aaacgagaca tcattaattc ctaccaatgc
      781 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
      841 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
      901 atgataccgc gagagccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
      961 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctatcaat
     1021 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     1081 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     1141 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     1201 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     1261 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     1321 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     1381 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     1441 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     1501 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     1561 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     1621 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     1681 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     1741 tttcccggga actgccagac atcaaataaa acaaaaggct cagtcggaag actgggcctt
     1801 ttgttttatc tgttgtttgt cggtgaacac tctcccgatt gcgatacaga ccctaatttc
     1861 acatcatatg acactaatcc taatttttgt tgacactcta tcattgatag agttatttta
     1921 cctctcccta tcagtgatag agaaaactag aaataatttt gtttaacttt aagaaggaga
     1981 tatacatatg gctagcagag gatcgcatca ccatcaccat cacatcgaag ggcgcatgtc
     2041 gaaatcatgg ggaaaattca tcgaggaaga agaggcggag atggcgtccc gccgcaacct
     2101 tatgattgtg gatggcacta acctggggtt tcgcttcaaa cacaacaata gcaaaaaacc
     2161 gttcgcctct agttacgtat ctacaattca gagtctggca aaatcttatt cggcgcgcac
     2221 tactatcgta ctgggcgata agggcaaaag tgtatttcgc ctggaacatt taccagaata
     2281 taagggcaat cgtgacgaga aatacgcgca acgcaccgaa gaggaaaagg cacttgatga
     2341 gcaattcttc gagtatttga aggatgcgtt cgaattgtgt aaaactactt tccccacttt
     2401 tacaattcgt ggtgtggagg ctgacgacat ggcagcatat attgttaaat tgattggaca
     2461 cctgtacgac cacgtatggt taattagcac agatggagat tgggacacac ttctgactga
     2521 taaggtgtct cgttttagtt ttaccactcg ccgtgagtat cacctgcgcg atatgtacga
     2581 acatcacaac gtagacgacg tggagcagtt catttcattg aaagcgatta tgggggattt
     2641 aggtgataac atccgtggag tggagggcat cggagcaaag cgcggctaca atatcatccg
     2701 cgaatttgga aatgtattgg atattattga ccagttacca ttaccgggta aacaaaagta
     2761 tatccaaaat ttgaacgcat ccgaggaatt gttatttcgc aacctgatct tagtcgattt
     2821 accaacatat tgcgtcgatg ctattgctgc tgtgggtcaa gacgttttgg acaaattcac
     2881 caaggatatt cttgagattg cggagcagtg ataattgcct ctgccaaaat tctgtcctca
     2941 agcgttttag ttcgtcggtc agtttcacct gatttacgta aaaacccgct tcggcgggtt
     3001 tttgcttttg gaggggcaga aagatgaatg actgtcggcc attcgatggt gtcgggtcat
     3061 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3121 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3181 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3241 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3301 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3361 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3421 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     3481 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     3541 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     3601 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     3661 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     3721 c
//
