LOCUS       Exported                8783 bp ds-DNA     circular SYN 20-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        48..353
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        354..557
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     regulatory      629..638
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
     CDS             635..1312
                     /codon_start=1
                     /product="humanized monomeric Azami-Green fluorescent
                     protein"
                     /label=hmAzami-Green
                     /note="mammalian codon-optimized"
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             1313..3655
                     /codon_start=1
                     /label=-beta-catenin
                     /translation="ATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSL
                     SGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDE
                     GMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDED
                     QVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSH
                     HREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKM
                     VALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTS
                     RVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEG
                     LLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDRED
                     ITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLA
                     LCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGAL
                     HILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGA
                     TAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETAD
                     LGLDIGAQGEALGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLP
                     DLGHAQDLMDGLPPGDSNQLAWFDTDL"
     polyA_signal    3676..3797
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3816..3836
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     protein_bind    3864..3884
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     protein_bind    3911..3931
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     enhancer        3943..4322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4323..4526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    4527..4548
                     /label=Twist_adapter_1
     CDS             4549..4815
                     /codon_start=1
                     /product="Golgi targeting sequence from EVROGEN 
                     MRLREPLLSGSAAMPGASLQRACRLLVAVCALHLGVTLVYYLAGRDLSRLPQLVGVSTP
                      LQGGSNSAAAIGQSSGELRTGGAKDPPVAT
                     pFusionRed-Golgi vectorcat.# FP419"
                     /label=GTS
                     /translation="MRLREPLLSGSAAMPGASLQRACRLLVAVCALHLGVTLVYYLAGR
                     DLSRLPQLVGVSTPLQGGSNSAAAIGQSSGELRTGGAKDPPVAT"
     CDS             4816..5511
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
     polyA_signal    5541..5675
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    5796..6384
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(6267..6278)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6400..6424)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6523..6556)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     rep_origin      6558..6914
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             7377..8168
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy,
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        8259..8368
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     misc_feature    8504..8552
                     /label=HSV TK poly(A) signal
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     protein_bind    join(8776..8783,1..17)
                     /gene="mutant version of lambda attB"
                     /label=attB1
                     /bound_moiety="BP Clonase"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtacaaaaaa gcaggctgtt taaacgatat ccccatatat ggagttccgc gttacataac
       61 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      121 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      181 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
      241 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
      301 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      361 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      421 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      481 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
      541 tctatataag cagagctgga attctgcagt cgatcgacgg taccgcgggc ccgggccact
      601 agtaacggcc gccagtgtgc tggaattcgc caccatggtg agcgtgatca agcccgagat
      661 gaagatcaag ctgtgcatga ggggcaccgt gaacggccac aacttcgtga tcgagggcga
      721 gggcaagggc aacccctacg agggcaccca gatcctggac ctgaacgtga ccgagggcgc
      781 ccccctgccc ttcgcctacg acatcctgac caccgtgttc cagtacggca acagggcctt
      841 caccaagtac cccgccgaca tccaggacta cttcaagcag accttccccg agggctacca
      901 ctgggagagg agcatgacct acgaggacca gggcatctgc accgccacca gcaacatcag
      961 catgaggggc gactgcttct tctacgacat caggttcgac ggcaccaact tcccccccaa
     1021 cggccccgtg atgcagaaga agaccctgaa gtgggagccc agcaccgaga agatgtacgt
     1081 ggaggacggc gtgctgaagg gcgacgtgaa catgaggctg ctgctggagg gcggcggcca
     1141 ctacaggtgc gacttcaaga ccacctacaa ggccaagaag gaggtgaggc tgcccgacgc
     1201 ccacaagatc gaccacagga tcgagatcct gaagcacgac aaggactaca acaaggtgaa
     1261 gctgtacgag aacgccgtgg ccaggtactc catgctgccc agccaggcca aggctactca
     1321 agctgacctg atggagttgg acatggccat ggagccggac agaaaagctg ctgtcagcca
     1381 ctggcagcag cagtcttact tggattctgg aatccattct ggtgccacca ccacagctcc
     1441 ttccctgagt ggcaagggca accctgagga agaagatgtt gacacctccc aagtccttta
     1501 tgaatgggag caaggctttt cccagtcctt cacgcaagag caagtagctg atattgacgg
     1561 gcagtatgca atgactaggg ctcagagggt ccgagctgcc atgttccctg agacgctaga
     1621 tgagggcatg cagatcccat ccacgcagtt tgacgctgct catcccacta atgtccagcg
     1681 cttggctgaa ccatcacaga tgttgaaaca tgcagttgtc aatttgatta actatcagga
     1741 tgacgcggaa cttgccacac gtgcaattcc tgagctgaca aaactgctaa acgatgagga
     1801 ccaggtggta gttaataaag ctgctgttat ggtccatcag ctttccaaaa aggaagcttc
     1861 cagacatgcc atcatgcgct cccctcagat ggtgtctgcc attgtacgca ccatgcagaa
     1921 tacaaatgat gtagagacag ctcgttgtac tgctgggact ctgcacaacc tttctcacca
     1981 ccgcgagggc ttgctggcca tctttaagtc tggtggcatc ccagcgctgg tgaaaatgct
     2041 tgggtcacca gtggattctg tactgttcta cgccatcacg acactgcata atctcctgct
     2101 ccatcaggaa ggagctaaaa tggcagtgcg cctagctggt ggactgcaga aaatggttgc
     2161 tttgctcaac aaaacaaacg tgaaattctt ggctattaca acagactgcc ttcagatctt
     2221 agcttatggc aatcaagaga gcaagctcat cattctggcc agtggtggac cccaagcctt
     2281 agtaaacata atgaggacct acacttatga gaagcttctg tggaccacaa gcagagtgct
     2341 gaaggtgctg tctgtctgct ctagcaacaa gccggccatt gtagaagctg gtgggatgca
     2401 ggcactgggg cttcatctga cagacccaag tcagcgactt gttcaaaact gtctttggac
     2461 tctcagaaac ctttcagatg cagcgactaa gcaggaaggg atggaaggcc tccttgggac
     2521 tctagtgcag cttctgggtt ccgatgatat aaatgtggtc acctgtgcag ctggaattct
     2581 ctctaacctc acttgcaata attacaaaaa caagatgatg gtgtgccaag tgggtggcat
     2641 agaggctctt gtacgcaccg tccttcgtgc tggtgacagg gaagacatca ctgagcctgc
     2701 catctgtgct cttcgtcatc tgaccagccg gcatcaggaa gccgagatgg cccagaatgc
     2761 cgttcgcctt cattatggac tgcctgttgt ggttaaactc ctgcacccac catcccactg
     2821 gcctctgata aaggcaactg ttggattgat tcgaaacctt gccctttgcc cagcaaatca
     2881 tgcgcctttg cgggaacagg gtgctattcc acgactagtt cagctgcttg tacgagcaca
     2941 tcaggacacc caacggcgca cctccatggg tggaacgcag cagcagtttg tggagggcgt
     3001 gcgcatggag gagatagtag aagggtgtac tggagctctc cacatccttg ctcgggacgt
     3061 tcacaaccgg attgtaatcc gaggactcaa taccattcca ttgtttgtgc agttgcttta
     3121 ttctcccatt gaaaatatcc aaagagtagc tgcaggggtc ctctgtgaac ttgctcagga
     3181 caaggaggct gcagaggcca ttgaagctga gggagccaca gctcccctga cagagttact
     3241 ccactccagg aatgaaggcg tggcaacata cgcagctgct gtcctattcc gaatgtctga
     3301 ggacaagcca caggattaca agaagcggct ttcagtcgag ctgaccagtt ccctcttcag
     3361 gacagagcca atggcttgga atgagactgc agatcttgga ctggacattg gtgcccaggg
     3421 agaagccctt ggatatcgcc aggatgatcc cagctaccgt tcttttcact ctggtggata
     3481 cggccaggat gccttgggga tggaccctat gatggagcat gagatgggtg gccaccaccc
     3541 tggtgctgac tatccagttg atgggctgcc tgatctggga cacgcccagg acctcatgga
     3601 tgggctgccc ccaggtgata gcaatcagct ggcctggttt gatactgacc tgtaatgaat
     3661 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3721 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3781 ctcatcaatg tatcttagat atccctaggc ccgggcaact ttgtataata aagttggttt
     3841 aaacgatatc cctaggcccg ggccaacttt gtatagaaaa gttggtttaa acgatatccc
     3901 taggcccggg caactttgta tacaaaagtt ggtttaaacg atgacattga ttattgacta
     3961 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     4021 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     4081 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     4141 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     4201 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     4261 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     4321 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     4381 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     4441 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     4501 ggtgggaggt ctatataagc agagctgaag tgccattccg cctgacctat gaggcttcgt
     4561 gaacccttgt tgtcaggctc tgctgccatg cccggtgcca gcctgcaaag ggcatgtcgt
     4621 cttctggttg cggtgtgtgc attgcatctc ggggtgactc ttgtgtatta tcttgcggga
     4681 agagatctct cccggcttcc acagttggtg ggcgtgagta cccccttgca aggcggttca
     4741 aatagcgctg cagctattgg acaaagcagc ggcgaactga ggacaggcgg cgctaaagac
     4801 ccaccagtag cgactagcga gctgattaag gagaacatgc acatgaagct gtacatggag
     4861 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     4921 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     4981 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
     5041 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     5101 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     5161 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
     5221 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
     5281 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa catcaagacc
     5341 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
     5401 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
     5461 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa tggatcctag
     5521 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     5581 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5641 ccaaactcat caatgtatct tatcatgtct ggatctgatc actgcttgag cctagaagat
     5701 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     5761 ctatcataac ccctagggta tacccatcta attggaatca acctctggat tacaaaattt
     5821 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     5881 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     5941 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     6001 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     6061 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     6121 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     6181 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     6241 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     6301 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     6361 tctccctttg ggccgcctcc ccgcatccct aggcccggga gacagctttc ttgtacaaag
     6421 tggttgataa gcttaaacgt cgagggatct tcataagaga agagggacag ctatgactgg
     6481 gagtagtcag gagaggagga aaaaaaccca tgtgcctggc agataacttc gtataatgta
     6541 tgctatacga agttatggta cgtactaagc tctcatgttt cacgtactaa gctctcatgt
     6601 ttaacgtact aagctctcat gtttaacgaa ctaaaccctc atggctaacg tactaagctc
     6661 tcatggctaa cgtactaagc tctcatgttt cacgtactaa gctctcatgt ttgaacaata
     6721 aaattaatat aaatcagcaa cttaaatagc ctctaaggtt ttaagtttta taagaaaaaa
     6781 aagaatatat aaggctttta aagcttttaa ggtttaacgg ttgtggacaa caagccaggg
     6841 atgtaacgca ctgagaagcc cttagagcct ctcaaagcaa ttttgagtga cacaggaaca
     6901 cttaacggct gacataattc agcttcacgc tgccgcaagc actcagggcg caagggctgc
     6961 taaaggaagc ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     7021 gtcagctggg aggcagaata aatgatcata tcgtcaatta ttacctccac ggggagagcc
     7081 tgagcaaact ggcctcaggc atttgagaag cacacggtca cactgcttcc ggtagtcaat
     7141 aaaccggtaa gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg
     7201 gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt ggggtacagt
     7261 ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat
     7321 ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatga
     7381 gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc
     7441 gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc
     7501 tgaagccaca cagtgatatt gatttgctgg ttacggtgac ggtaaggctt gatgaaacaa
     7561 cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga
     7621 ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc
     7681 cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct
     7741 tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata
     7801 gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc
     7861 tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg
     7921 atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa
     7981 tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc
     8041 ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc
     8101 gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg
     8161 gcaaataatg tctaacaatt cgttcaagcc gacggatcta tgtcgggtgc ggagaaagag
     8221 gtaatgaaat ggcacctagg tatcgatact agtatacgga cctttaattc aacccaacac
     8281 aatatattat agttaaataa gaattattat caaatcattt gtatattaat taaaatacta
     8341 tactgtaaat tacattttat ttacaatcac tcgacgaaga cttgatcacc cgggatctcg
     8401 agccatggtg ctagcagctg atgcatagca tgcggtaccg ggagatgggg gaggctaact
     8461 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag
     8521 aataaaacgc acgggtgttg ggtcgtttgt tcataaacgc ggggttcggt cccagggctg
     8581 gcactctgtc gataccccac cgagacccca ttgggaccaa tacgcccgcg tttcttcctt
     8641 ttccccaccc caacccccaa gttcgggtga aggcccaggg ctcgcagcca acgtcggggc
     8701 ggcaagccct gccatagcca ctacgggtac gtttctgtaa ctataacggt cctaaggtag
     8761 cgagtttaaa catcaacaag ttt
//
