LOCUS       Exported               18125 bp ds-DNA     circular SYN 20-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18125)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..18125
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    1..34
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    76..100
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    127..147
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        165..405
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    414..433
                     /label=HEK3 spacer
     misc_RNA        434..509
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    510..519
                     /label=RT primer
     misc_feature    complement(523..535)
                     /label=HEK3 primer binding site
     protein_bind    579..599
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        608..699
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             791..1486
                     /codon_start=2
                     /product="IGEM parts BBa_K864401"
                     /label=aeBlue
                     /translation="MLHWLKKTCASK*RWKEQ*TVTISSV*EKAKANHLKGPRWKRYAS
                     LKVGPYHLRMIFWPLVACMAVKPSLSMCRVFRITLRSLFLRALPGKEHKSSRMAAISPY
                     TRTRAFRVIILFSKLMSSVPTSLQTVP*CRKKQLDGNRALRCFIRGTASCVVRA*WP*N
                     ALMAII*RPTCALPIVLASHPMQLTCRNFILGIIALRF*KLNKVNFMNNTSQRWPVTVR
                     RHRVN*GIT"
     polyA_signal    1697..1831
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1855..1946
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             2018..3553
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    3746..3880
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3989..4009
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     enhancer        4021..4400
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4401..4604
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          4740..5009
                     /label=CMV intron
                     /note="modified intron A from human cytomegalovirus"
     CDS             5060..5758
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    5768..6356
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(6239..6250)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6372..6396)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        6445..6824
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6825..7028
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        7070..7088
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7138..7158
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7159..11259
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             11359..13390
                     /codon_start=1
                     /product="https://www.addgene.org/132775/sequences/"
                     /label=MoMLV RT PE2
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     VDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPLPDA
                     DHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIALTQALKMAEGK
                     KLNVYTDSRYAFATAHIHGEIYRRRGWLTSEGKEIKNKDEILALLKALFLPKRLSIIHC
                     PGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSP"
     CDS             13432..13452
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13461..13478
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    13510..13644
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  13765..13930
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     misc_feature    14047..14096
                     /label=IRL Tn3
     rep_origin      14120..14708
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  14843..15066
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(15133..15666)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(15855..15883)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    15954..15987
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_RNA        complement(16056..16131)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    complement(16132..16151)
                     /label=HEK3_4a_+90
     promoter        complement(16160..16400)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     promoter        16560..16662
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             16663..17322
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     rep_origin      17717..18073
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
ORIGIN
        1 ataacttcgt atagcataca ttatacgaag ttatctgtaa ctataacggt cctaaggtag
       61 cgagtttaaa catcaacaag tttgtacaaa aaagcaggct gtttaaacga tatccctagg
      121 cccgggcaac tttgtataat aaagttggtt taaacgatat caccgagggc ctatttccca
      181 tgattccttc atatttgcat atacgataca aggctgttag agagataatt agaattaatt
      241 tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
      301 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
      361 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accggcccag
      421 actgagcacg tgagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      481 aacttgaaaa agtgggaccg agtcggtcct ctgccatcaa agcgtgctca gtctgttttt
      541 ttaagcttgg gccgctcgag gtatccctag gcccgggcca actttgtata gaaaagttgg
      601 tttaaacatc atggagataa ttaaaatgat aaccatctcg caaataaata agtattttac
      661 tgttttcgta acagttttgt aataaaaaaa cctataaata ttccggatta ttcataccgt
      721 cccaccatcg ggcgcggatc ccggtccgaa gcgcgcggaa ttcaaaggga agtgccattc
      781 cgcctgacct atggcttcac tggttaaaaa agacatgtgc atcaaaatga cgatggaagg
      841 aacagtaaac ggtcaccatt tcaagtgtgt aggagaaggc gaaggcaaac catttgaagg
      901 gacccaggtg gaaaagatac gcatcactga aggtgggccc ttaccatttg cgtatgatat
      961 tttggcccct tgttgcatgt atggcagtaa aaccttcatt aagcatgtgt cgggtattcc
     1021 ggattacttt aaggagtctt ttcctgaggg ctttacctgg gaaagaacac aaatcttcga
     1081 ggatggcggc tatctcacca tacaccagga cacgagcctt cagggtaata attttatttt
     1141 caaagttaat gtcatcggtg ccaacttccc tgcaaacggt cccgtgatgc agaaaaaaac
     1201 agctggatgg gaaccgtgcg ttgagatgct ttatccgcgg gacggcgtcc tgtgtggtca
     1261 gagcctgatg gccctgaaat gcactgatgg caatcatctg acgtcccacc tgcgcactac
     1321 ctatcgttct cgcaagccat ccaatgcagt taacatgccg gaatttcatt ttggggatca
     1381 tcgcattgag attttgaaag ctgaacaagg taaattttat gaacaatacg agtcagcggt
     1441 ggcccgttac tgtgaggcgg caccgagtaa attagggcat cactaaaggc taggtggagg
     1501 ctcagtgcct acgtcgacga gctcacttgt cgcggccgct ttcgaatcta gagcctgcag
     1561 tctcgacaag cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga
     1621 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     1681 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     1741 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1801 actcatcaat gtatcttatc atgtctggat cgacctactc cggaatatta atagatcatg
     1861 gagataatta aaatgataac catctcgcaa ataaataagt attttactgt tttcgtaaca
     1921 gttttgtaat aaaaaaacct ataaatattc cggattattc ataccgtccc accatcgggc
     1981 gcggatcccg gtccgaagcg cgcggaattc aaaggccatg aagtgccttt tgtacttagc
     2041 ctttttattc attggggtga attgcaagtt caccatagtt tttccacaca accaaaaagg
     2101 aaactggaaa aatgttcctt ctaattacca ttattgcccg tcaagctcag atttaaattg
     2161 gcataatgac ttaataggca cagccttaca agtcaaaatg cccaagagtc acaaggctat
     2221 tcaagcagac ggttggatgt gtcatgcttc caaatgggtc actacttgtg atttccgctg
     2281 gtatggaccg aagtatataa cacattccat ccgatccttc actccatctg tagaacaatg
     2341 caaggaaagc attgaacaaa cgaaacaagg aacttggctg aatccaggct tccctcctca
     2401 aagttgtgga tatgcaactg tgacggatgc cgaagcagtg attgtccagg tgactcctca
     2461 ccatgtgctg gttgatgaat acacaggaga atgggttgat tcacagttca tcaacggaaa
     2521 atgcagcaat tacatatgcc ccactgtcca taactctaca acctggcatt ctgactataa
     2581 ggtcaaaggg ctatgtgatt ctaacctcat ttccatggac atcaccttct tctcagagga
     2641 cggagagcta tcatccctgg gaaaggaggg cacagggttc agaagtaact actttgctta
     2701 tgaaactgga ggcaaggcct gcaaaatgca atactgcaag cattggggag tcagactccc
     2761 atcaggtgtc tggttcgaga tggctgataa ggatctcttt gctgcagcca gattccctga
     2821 atgcccagaa gggtcaagta tctctgctcc atctcagacc tcagtggatg taagtctaat
     2881 tcaggacgtt gagaggatct tggattattc cctctgccaa gaaacctgga gcaaaatcag
     2941 agcgggtctt ccaatctctc cagtggatct cagctatctt gctcctaaaa acccaggaac
     3001 cggtcctgct ttcaccataa tcaatggtac cctaaaatac tttgagacca gatacatcag
     3061 agtcgatatt gctgctccaa tcctctcaag aatggtcgga atgatcagtg gaactaccac
     3121 agaaagggaa ctgtgggatg actgggcacc atatgaagac gtggaaattg gacccaatgg
     3181 agttctgagg accagttcag gatataagtt tcctttatac atgattggac atggtatgtt
     3241 ggactccgat cttcatctta gctcaaaggc tcaggtgttc gaacatcctc acattcaaga
     3301 cgctgcttcg caacttcctg atgatgagag tttatttttt ggtgatactg ggctatccaa
     3361 aaatccaatc gagcttgtag aaggttggtt cagtagttgg aaaagctcta ttgcctcttt
     3421 tttctttatc atagggttaa tcattggact attcttggtt ctccgagttg gtatccatct
     3481 ttgcattaaa ttaaagcaca ccaagaaaag acagatttat acagacatag agatgaaccg
     3541 acttggaaag tgataaccta cgtcgacgag ctcacttgtc gcggccgctt tcgaatctag
     3601 agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg atcataatca gccataccac
     3661 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     3721 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     3781 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     3841 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag
     3901 aagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc
     3961 aataactatc ataaccccta ggcccgggca actttgtata caaaagttgg tttaaacgat
     4021 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     4081 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     4141 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     4201 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     4261 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     4321 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     4381 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     4441 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     4501 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     4561 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
     4621 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
     4681 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
     4741 taagtaccgc ctatagactc tataggcaca cccctttggc tcttatgcat gctatactgt
     4801 ttttggcttg gggcctatac acccccgctt ccttatgcta taggtgatgg tatagcttag
     4861 cctataggtg tgggttattg accattattg accactccaa cggtggaggg cagtgtagtc
     4921 tgagcagtac tcgttgctgc cgcgcgcgcc accagacata atagctgaca gactaacaga
     4981 ctgttccttt ccatgggtct tttctgcagt caccgtcgtc gacggtatcg ataagcttga
     5041 tatcgaattc gccgccgcca tgagcgagct gattaaggag aacatgcaca tgaagctgta
     5101 catggagggc accgtggaca accatcactt caagtgcaca tccgagggcg aaggcaagcc
     5161 ctacgagggc acccagacca tgagaatcaa ggtggtcgag ggcggccctc tccccttcgc
     5221 cttcgacatc ctggctacta gcttcctcta cggcagcaag accttcatca accacaccca
     5281 gggcatcccc gacttcttca agcagtcctt ccctgagggc ttcacatggg agagagtcac
     5341 cacatacgaa gacgggggcg tgctgaccgc tacccaggac accagcctcc aggacggctg
     5401 cctcatctac aacgtcaaga tcagaggggt gaacttcaca tccaacggcc ctgtgatgca
     5461 gaagaaaaca ctcggctggg aggccttcac cgagacgctg taccccgctg acggcggcct
     5521 ggaaggcaga aacgacatgg ccctgaagct cgtgggcggg agccatctga tcgcaaacat
     5581 caagaccaca tatagatcca agaaacccgc taagaacctc aagatgcctg gcgtctacta
     5641 tgtggactac agactggaaa gaatcaagga ggccaacaac gagacctacg tcgagcagca
     5701 cgaggtggca gtggccagat actgcgacct ccctagcaaa ctggggcaca agcttaatgg
     5761 atcctagaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     5821 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     5881 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5941 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6001 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6061 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6121 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     6181 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6241 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6301 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcc
     6361 ctaggcccgg gagacagctt tcttgtacaa agtggttgat aagcttaaac actagtatcg
     6421 atcgcgatgt acgggccaga tatagacatt gattattgac tagttattaa tagtaatcaa
     6481 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
     6541 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     6601 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     6661 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     6721 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     6781 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     6841 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     6901 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     6961 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     7021 gcagagctgg tttagtgaac cgtcagatcc gctagagatc cgcggccgct aatacgactc
     7081 actataggga gagccgccac catgaaacgg acagccgacg gaagcgagtt cgagtcacca
     7141 aagaagaagc ggaaagtcga caagaagtac agcatcggcc tggacatcgg caccaactct
     7201 gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg
     7261 ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc
     7321 ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg
     7381 aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac
     7441 agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg
     7501 caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc
     7561 taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat
     7621 ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac
     7681 cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg
     7741 ttcgaggaaa accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga
     7801 ctgagcaaga gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat
     7861 ggcctgttcg gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac
     7921 ttcgacctgg ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg
     7981 gacaacctgc tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac
     8041 ctgtccgacg ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc
     8101 cccctgagcg cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg
     8161 aaagctctcg tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc
     8221 aagaacggct acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc
     8281 atcaagccca tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga
     8341 gaggacctgc tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac
     8401 ctgggagagc tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac
     8461 aaccgggaaa agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg
     8521 gccaggggaa acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc
     8581 tggaacttcg aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg
     8641 accaacttcg ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac
     8701 gagtacttca ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga
     8761 aagcccgcct tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc
     8821 aaccggaaag tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc
     8881 gactccgtgg aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac
     8941 gatctgctga aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt
     9001 ctggaagata tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg
     9061 ctgaaaacct atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga
     9121 tacaccggct ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc
     9181 ggcaagacaa tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag
     9241 ctgatccacg acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc
     9301 cagggcgata gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag
     9361 ggcatcctgc agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag
     9421 cccgagaaca tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag
     9481 aacagccgcg agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc
     9541 ctgaaagaac accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac
     9601 ctgcagaatg ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac
     9661 tacgatgtgg acgctatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag
     9721 gtgctgacca gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc
     9781 gtgaagaaga tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga
     9841 aagttcgaca atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc
     9901 ttcatcaaga gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg
     9961 gactcccgga tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg
    10021 atcaccctga agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg
    10081 cgcgagatca acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc
    10141 gccctgatca aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg
    10201 tacgacgtgc ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag
    10261 tacttcttct acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc
    10321 gagatccgga agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat
    10381 aagggccggg attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg
    10441 aaaaagaccg aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac
    10501 agcgataagc tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac
    10561 agccccaccg tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag
    10621 aaactgaaga gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag
    10681 aagaatccca tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc
    10741 atcaagctgc ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc
    10801 tctgccggcg aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc
    10861 ctgtacctgg ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa
    10921 cagctgtttg tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag
    10981 ttctccaaga gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac
    11041 aagcaccggg ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg
    11101 accaatctgg gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg
    11161 tacaccagca ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg
    11221 tacgagacac ggatcgacct gtctcagctg ggaggtgact ctggaggatc tagcggagga
    11281 tcctctggca gcgagacacc aggaacaagc gagtcagcaa caccagagag cagtggcggc
    11341 agcagcggcg gcagcagcac cctaaatata gaagatgagt atcggctaca tgagacctca
    11401 aaagagccag atgtttctct agggtccaca tggctgtctg attttcctca ggcctgggcg
    11461 gaaaccgggg gcatgggact ggcagttcgc caagctcctc tgatcatacc tctgaaagca
    11521 acctctaccc ccgtgtccat aaaacaatac cccatgtcac aagaagccag actggggatc
    11581 aagccccaca tacagagact gttggaccag ggaatactgg taccctgcca gtccccctgg
    11641 aacacgcccc tgctacccgt taagaaacca gggactaatg attataggcc tgtccaggat
    11701 ctgagagaag tcaacaagcg ggtggaagac atccacccca ccgtgcccaa cccttacaac
    11761 ctcttgagcg ggctcccacc gtcccaccag tggtacactg tgcttgattt aaaggatgcc
    11821 tttttctgcc tgagactcca ccccaccagt cagcctctct tcgcctttga gtggagagat
    11881 ccagagatgg gaatctcagg acaattgacc tggaccagac tcccacaggg tttcaaaaac
    11941 agtcccaccc tgtttaatga ggcactgcac agagacctag cagacttccg gatccagcac
    12001 ccagacttga tcctgctaca gtacgtggat gacttactgc tggccgccac ttctgagcta
    12061 gactgccaac aaggtactcg ggccctgtta caaaccctag ggaacctcgg gtatcgggcc
    12121 tcggccaaga aagcccaaat ttgccagaaa caggtcaagt atctggggta tcttctaaaa
    12181 gagggtcaga gatggctgac tgaggccaga aaagagactg tgatggggca gcctactccg
    12241 aagacccctc gacaactaag ggagttccta gggaaggcag gcttctgtcg cctcttcatc
    12301 cctgggtttg cagaaatggc agcccccctg taccctctca ccaaaccggg gactctgttt
    12361 aattggggcc cagaccaaca aaaggcctat caagaaatca agcaagctct tctaactgcc
    12421 ccagccctgg ggttgccaga tttgactaag ccctttgaac tctttgtcga cgagaagcag
    12481 ggctacgcca aaggtgtcct aacgcaaaaa ctgggacctt ggcgtcggcc ggtggcctac
    12541 ctgtccaaaa agctagaccc agtagcagct gggtggcccc cttgcctacg gatggtagca
    12601 gccattgccg tactgacaaa ggatgcaggc aagctaacca tgggacagcc actagtcatt
    12661 ctggcccccc atgcagtaga ggcactagtc aaacaacccc ccgaccgctg gctttccaac
    12721 gcccggatga ctcactatca ggccttgctt ttggacacgg accgggtcca gttcggaccg
    12781 gtggtagccc tgaacccggc tacgctgctc ccactgcctg aggaagggct gcaacacaac
    12841 tgccttgata tcctggccga agcccacgga acccgacccg acctaacgga ccagccgctc
    12901 ccagacgccg accacacctg gtacacggat ggaagcagtc tcttacaaga gggacagcgt
    12961 aaggcgggag ctgcggtgac caccgagacc gaggtaatct gggctaaagc cctgccagcc
    13021 gggacatccg ctcagcgggc tgaactgata gcactcaccc aggccctaaa gatggcagaa
    13081 ggtaagaagc taaatgttta tactgatagc cgttatgctt ttgctactgc ccatatccat
    13141 ggagaaatat acagaaggcg tgggtggctc acatcagaag gcaaagagat caaaaataaa
    13201 gacgagatct tggccctact aaaagccctc tttctgccca aaagacttag cataatccat
    13261 tgtccaggac atcaaaaggg acacagcgcc gaggctagag gcaaccggat ggctgaccaa
    13321 gcggcccgaa aggcagccat cacagagact ccagacacct ctaccctcct catagaaaat
    13381 tcatcaccct ctggcggctc aaaaagaacc gccgacggca gcgaattcga gcccaagaag
    13441 aagaggaaag tctaaccggt catcatcacc atcaccattg agtttaaact gaatgcaatt
    13501 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
    13561 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
    13621 aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctagaagatc cggctgctaa
    13681 caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tatcataacc
    13741 cctagggtat acccatctaa ttggaaccag ataagtgaaa tctagttcca aactattttg
    13801 tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct attttgtcac
    13861 tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc aactattttg
    13921 tccgcccaca accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt
    13981 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    14041 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    14101 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    14161 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    14221 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    14281 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    14341 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    14401 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    14461 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    14521 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    14581 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    14641 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    14701 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    14761 ctcacatgtt ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc
    14821 gcagatgccc tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag
    14881 atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc
    14941 cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt
    15001 tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact
    15061 atattcgcgg cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc
    15121 ttgaacgaat tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg
    15181 tatgcccaac tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta
    15241 gatcacataa gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc
    15301 aatgccctgc ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg
    15361 cggctgctca aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc
    15421 gaaggcagca agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc
    15481 cggctgatgt tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc
    15541 agcccgcatg gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact
    15601 tgagccacct aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg
    15661 taacatcgtt gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct
    15721 tggatgcccg aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca
    15781 ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac
    15841 gctacttgca ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca
    15901 tccgtttcca cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt
    15961 cgtatagcat acattatacg aagttatctg ccaggcacat gggttttact agtatcgatt
    16021 cgcgatgtac gggcccgggc ctagggataa aaaaagcacc gactcggtgc cactttttca
    16081 agttgataac ggactagcct tattttaact tgctatttct agctctaaaa cgcaccggga
    16141 tactggttga cggtgtttcg tcctttccac aagatatata aagccaagaa atcgaaatac
    16201 tttcaagtta cggtaagcat atgatagtcc attttaaaac ataattttaa aactgcaaac
    16261 tacccaagaa attattactt tctacgtcac gtattttgta ctaatatctt tgtgtttaca
    16321 gtcaaattaa ttccaattat ctctctaaca gccttgtatc gtatatgcaa atatgaagga
    16381 atcatgggaa ataggccctc ggtgatatcg gatccggctg ctaacaaagc ccgaaaggaa
    16441 gctgagttgg ctgctgccac cgctgagcaa taactatcat aacccctagg tgccatttca
    16501 ttacctcttt ctccgcaccc gacatagatc tgggccaact tttggcgaaa atgagacgtt
    16561 gatcggcacg taagagggtc caactttcac cataatgaaa taagatcact accgggcgta
    16621 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
    16681 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
    16741 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
    16801 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
    16861 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
    16921 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
    16981 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
    17041 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
    17101 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
    17161 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
    17221 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
    17281 gaattacaac agtactgcga tgagtggcag ggcggggcgt aattttttta aggcagttat
    17341 tggtgccctt aaacgcctgg ttgctacgcc tgaataagtg ataataagcg gatgaatggc
    17401 agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt taaatagccg
    17461 cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg accgtgtgct
    17521 tctcaaatgc ctgaggccag tttgctcagg ctctccccgt ggaggtaata attgacgata
    17581 tgatcattta ttctgcctcc cagctgacat tcatccgggg tcagcaccgt ttctgcggac
    17641 tggctttcta cgtgttccgc ttcctttagc agcccttgcg ccctgagtgc ttgcggcagc
    17701 gtgaagctaa ttcccatgtc agccgttaag tgttcctgtg tcactcaaaa ttgctttgag
    17761 aggctctaag ggcttctcag tgcgttacat ccctggcttg ttgtccacaa ccgttaaacc
    17821 ttaaaagctt taaaagcctt atatattctt ttttttctta taaaacttaa aaccttagag
    17881 gctatttaag ttgctgattt atattaattt tattgttcaa acatgagagc ttagtacgtg
    17941 aaacatgaga gcttagtacg ttagccatga gagcttagta cgttagccat gagggtttag
    18001 ttcgttaaac atgagagctt agtacgttaa acatgagagc ttagtacgtg aaacatgaga
    18061 gcttagtacg tactatcaac aggttgaact gctgatcaac agatcctcta cgcggccgcg
    18121 gtacc
//
