LOCUS       Exported               15953 bp ds-DNA     circular SYN 20-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15953)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 21, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15953
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..18
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    50..184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  305..470
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      660..1248
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  1383..1606
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1673..2206)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(2395..2423)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    2494..2527
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    2569..2593
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    2620..2640
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        2658..2898
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2907..2926
                     /label=HEK3 spacer
     misc_RNA        2927..3002
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    3003..3012
                     /label=RT primer
     misc_feature    complement(3016..3028)
                     /label=HEK3 primer binding site
     protein_bind    3072..3092
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        3101..3192
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             3284..3979
                     /codon_start=2
                     /product="IGEM parts BBa_K864401"
                     /label=aeBlue
                     /translation="MLHWLKKTCASK*RWKEQ*TVTISSV*EKAKANHLKGPRWKRYAS
                     LKVGPYHLRMIFWPLVACMAVKPSLSMCRVFRITLRSLFLRALPGKEHKSSRMAAISPY
                     TRTRAFRVIILFSKLMSSVPTSLQTVP*CRKKQLDGNRALRCFIRGTASCVVRA*WP*N
                     ALMAII*RPTCALPIVLASHPMQLTCRNFILGIIALRF*KLNKVNFMNNTSQRWPVTVR
                     RHRVN*GIT"
     polyA_signal    4190..4324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4348..4439
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4511..6046
                     /codon_start=1
                     /product="vesicular stomatitis virus G glycoprotein"
                     /label=VSV-G
                     /note="Indiana strain"
                     /translation="MKCLLYLAFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
                     LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
                     VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
                     FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
                     SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
                     SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
                     YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
                     LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
                     SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
     polyA_signal    6239..6373
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6482..6502
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     enhancer        6514..6893
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6894..7097
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          7233..7502
                     /label=CMV intron
                     /note="modified intron A from human cytomegalovirus"
     CDS             7553..8251
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    8261..8849
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     CDS             complement(8732..8743)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(8865..8889)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        8938..9317
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9318..9521
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        9563..9581
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9631..9651
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9652..13752
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(H840A)
                     /note="generates RNA-guided single strand nicks in DNA due
                     to the H840A mutation in the HNH catalytic domain"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             13852..15883
                     /codon_start=1
                     /product="https://www.addgene.org/132775/sequences/"
                     /label=MoMLV RT PE2
                     /translation="TLNIEDEYRLHETSKEPDVSLGSTWLSDFPQAWAETGGMGLAVRQ
                     APLIIPLKATSTPVSIKQYPMSQEARLGIKPHIQRLLDQGILVPCQSPWNTPLLPVKKP
                     GTNDYRPVQDLREVNKRVEDIHPTVPNPYNLLSGLPPSHQWYTVLDLKDAFFCLRLHPT
                     SQPLFAFEWRDPEMGISGQLTWTRLPQGFKNSPTLFNEALHRDLADFRIQHPDLILLQY
                     VDDLLLAATSELDCQQGTRALLQTLGNLGYRASAKKAQICQKQVKYLGYLLKEGQRWLT
                     EARKETVMGQPTPKTPRQLREFLGKAGFCRLFIPGFAEMAAPLYPLTKPGTLFNWGPDQ
                     QKAYQEIKQALLTAPALGLPDLTKPFELFVDEKQGYAKGVLTQKLGPWRRPVAYLSKKL
                     DPVAAGWPPCLRMVAAIAVLTKDAGKLTMGQPLVILAPHAVEALVKQPPDRWLSNARMT
                     HYQALLLDTDRVQFGPVVALNPATLLPLPEEGLQHNCLDILAEAHGTRPDLTDQPLPDA
                     DHTWYTDGSSLLQEGQRKAGAAVTTETEVIWAKALPAGTSAQRAELIALTQALKMAEGK
                     KLNVYTDSRYAFATAHIHGEIYRRRGWLTSEGKEIKNKDEILALLKALFLPKRLSIIHC
                     PGHQKGHSAEARGNRMADQAARKAAITETPDTSTLLIENSSP"
     CDS             15925..15945
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 catcatcacc atcaccattg agtttaaact gaatgcaatt gttgttgtta acttgtttat
       61 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
      121 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
      181 gatctgatca ctgcttgagc ctagaagatc cggctgctaa caaagcccga aaggaagctg
      241 agttggctgc tgccaccgct gagcaataac tatcataacc cctagggtat acccatctaa
      301 ttggaaccag ataagtgaaa tctagttcca aactattttg tcatttttaa ttttcgtatt
      361 agcttacgac gctacaccca gttcccatct attttgtcac tcttccctaa ataatcctta
      421 aaaactccat ttccacccct cccagttccc aactattttg tccgcccaca accggttgac
      481 ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
      541 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
      601 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
      661 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
      721 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
      781 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
      841 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
      901 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
      961 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1021 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     1081 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     1141 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     1201 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     1261 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     1321 ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc tgcgtaagcg
     1381 ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta tgacaataaa
     1441 gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct gtgaaaaagc
     1501 atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc aaattgcccg
     1561 tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg cgttgtgaca
     1621 atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg
     1681 cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga
     1741 gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg
     1801 cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct
     1861 cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca
     1921 gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga
     1981 atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg
     2041 tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt
     2101 ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt
     2161 tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat
     2221 aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac
     2281 tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg
     2341 cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta
     2401 cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt
     2461 cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat acattatacg
     2521 aagttatctg taactataac ggtcctaagg tagcgagttt aaacatcaac aagtttgtac
     2581 aaaaaagcag gctgtttaaa cgatatccct aggcccgggc aactttgtat aataaagttg
     2641 gtttaaacga tatcaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat
     2701 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac
     2761 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt
     2821 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat
     2881 atatcttgtg gaaaggacga aacaccggcc cagactgagc acgtgagttt tagagctaga
     2941 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggga ccgagtcggt
     3001 cctctgccat caaagcgtgc tcagtctgtt tttttaagct tgggccgctc gaggtatccc
     3061 taggcccggg ccaactttgt atagaaaagt tggtttaaac atcatggaga taattaaaat
     3121 gataaccatc tcgcaaataa ataagtattt tactgttttc gtaacagttt tgtaataaaa
     3181 aaacctataa atattccgga ttattcatac cgtcccacca tcgggcgcgg atcccggtcc
     3241 gaagcgcgcg gaattcaaag ggaagtgcca ttccgcctga cctatggctt cactggttaa
     3301 aaaagacatg tgcatcaaaa tgacgatgga aggaacagta aacggtcacc atttcaagtg
     3361 tgtaggagaa ggcgaaggca aaccatttga agggacccag gtggaaaaga tacgcatcac
     3421 tgaaggtggg cccttaccat ttgcgtatga tattttggcc ccttgttgca tgtatggcag
     3481 taaaaccttc attaagcatg tgtcgggtat tccggattac tttaaggagt cttttcctga
     3541 gggctttacc tgggaaagaa cacaaatctt cgaggatggc ggctatctca ccatacacca
     3601 ggacacgagc cttcagggta ataattttat tttcaaagtt aatgtcatcg gtgccaactt
     3661 ccctgcaaac ggtcccgtga tgcagaaaaa aacagctgga tgggaaccgt gcgttgagat
     3721 gctttatccg cgggacggcg tcctgtgtgg tcagagcctg atggccctga aatgcactga
     3781 tggcaatcat ctgacgtccc acctgcgcac tacctatcgt tctcgcaagc catccaatgc
     3841 agttaacatg ccggaatttc attttgggga tcatcgcatt gagattttga aagctgaaca
     3901 aggtaaattt tatgaacaat acgagtcagc ggtggcccgt tactgtgagg cggcaccgag
     3961 taaattaggg catcactaaa ggctaggtgg aggctcagtg cctacgtcga cgagctcact
     4021 tgtcgcggcc gctttcgaat ctagagcctg cagtctcgac aagcttgtcg agaagtacta
     4081 gaggatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     4141 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     4201 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     4261 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     4321 gatcgaccta ctccggaata ttaatagatc atggagataa ttaaaatgat aaccatctcg
     4381 caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa cctataaata
     4441 ttccggatta ttcataccgt cccaccatcg ggcgcggatc ccggtccgaa gcgcgcggaa
     4501 ttcaaaggcc atgaagtgcc ttttgtactt agccttttta ttcattgggg tgaattgcaa
     4561 gttcaccata gtttttccac acaaccaaaa aggaaactgg aaaaatgttc cttctaatta
     4621 ccattattgc ccgtcaagct cagatttaaa ttggcataat gacttaatag gcacagcctt
     4681 acaagtcaaa atgcccaaga gtcacaaggc tattcaagca gacggttgga tgtgtcatgc
     4741 ttccaaatgg gtcactactt gtgatttccg ctggtatgga ccgaagtata taacacattc
     4801 catccgatcc ttcactccat ctgtagaaca atgcaaggaa agcattgaac aaacgaaaca
     4861 aggaacttgg ctgaatccag gcttccctcc tcaaagttgt ggatatgcaa ctgtgacgga
     4921 tgccgaagca gtgattgtcc aggtgactcc tcaccatgtg ctggttgatg aatacacagg
     4981 agaatgggtt gattcacagt tcatcaacgg aaaatgcagc aattacatat gccccactgt
     5041 ccataactct acaacctggc attctgacta taaggtcaaa gggctatgtg attctaacct
     5101 catttccatg gacatcacct tcttctcaga ggacggagag ctatcatccc tgggaaagga
     5161 gggcacaggg ttcagaagta actactttgc ttatgaaact ggaggcaagg cctgcaaaat
     5221 gcaatactgc aagcattggg gagtcagact cccatcaggt gtctggttcg agatggctga
     5281 taaggatctc tttgctgcag ccagattccc tgaatgccca gaagggtcaa gtatctctgc
     5341 tccatctcag acctcagtgg atgtaagtct aattcaggac gttgagagga tcttggatta
     5401 ttccctctgc caagaaacct ggagcaaaat cagagcgggt cttccaatct ctccagtgga
     5461 tctcagctat cttgctccta aaaacccagg aaccggtcct gctttcacca taatcaatgg
     5521 taccctaaaa tactttgaga ccagatacat cagagtcgat attgctgctc caatcctctc
     5581 aagaatggtc ggaatgatca gtggaactac cacagaaagg gaactgtggg atgactgggc
     5641 accatatgaa gacgtggaaa ttggacccaa tggagttctg aggaccagtt caggatataa
     5701 gtttccttta tacatgattg gacatggtat gttggactcc gatcttcatc ttagctcaaa
     5761 ggctcaggtg ttcgaacatc ctcacattca agacgctgct tcgcaacttc ctgatgatga
     5821 gagtttattt tttggtgata ctgggctatc caaaaatcca atcgagcttg tagaaggttg
     5881 gttcagtagt tggaaaagct ctattgcctc ttttttcttt atcatagggt taatcattgg
     5941 actattcttg gttctccgag ttggtatcca tctttgcatt aaattaaagc acaccaagaa
     6001 aagacagatt tatacagaca tagagatgaa ccgacttgga aagtgataac ctacgtcgac
     6061 gagctcactt gtcgcggccg ctttcgaatc tagagcctgc agtctcgaca agcttgtcga
     6121 gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     6181 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     6241 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     6301 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6361 tcatgtctgg atctgatcac tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa
     6421 aggaagctga gttggctgct gccaccgctg agcaataact atcataaccc ctaggcccgg
     6481 gcaactttgt atacaaaagt tggtttaaac gatgacattg attattgact agttattaat
     6541 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     6601 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     6661 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     6721 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     6781 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     6841 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     6901 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     6961 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     7021 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     7081 tctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     7141 gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggccgg gaacggtgca
     7201 ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga ctctataggc
     7261 acaccccttt ggctcttatg catgctatac tgtttttggc ttggggccta tacacccccg
     7321 cttccttatg ctataggtga tggtatagct tagcctatag gtgtgggtta ttgaccatta
     7381 ttgaccactc caacggtgga gggcagtgta gtctgagcag tactcgttgc tgccgcgcgc
     7441 gccaccagac ataatagctg acagactaac agactgttcc tttccatggg tcttttctgc
     7501 agtcaccgtc gtcgacggta tcgataagct tgatatcgaa ttcgccgccg ccatgagcga
     7561 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtgg acaaccatca
     7621 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
     7681 caaggtggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ctagcttcct
     7741 ctacggcagc aagaccttca tcaaccacac ccagggcatc cccgacttct tcaagcagtc
     7801 cttccctgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
     7861 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
     7921 ggtgaacttc acatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctt
     7981 caccgagacg ctgtaccccg ctgacggcgg cctggaaggc agaaacgaca tggccctgaa
     8041 gctcgtgggc gggagccatc tgatcgcaaa catcaagacc acatatagat ccaagaaacc
     8101 cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac tacagactgg aaagaatcaa
     8161 ggaggccaac aacgagacct acgtcgagca gcacgaggtg gcagtggcca gatactgcga
     8221 cctccctagc aaactggggc acaagcttaa tggatcctag aatcaacctc tggattacaa
     8281 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     8341 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     8401 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     8461 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     8521 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     8581 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     8641 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     8701 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     8761 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     8821 tcggatctcc ctttgggccg cctccccgca tccctaggcc cgggagacag ctttcttgta
     8881 caaagtggtt gataagctta aacactagta tcgatcgcga tgtacgggcc agatatagac
     8941 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     9001 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     9061 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     9121 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     9181 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     9241 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     9301 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     9361 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     9421 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     9481 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga
     9541 tccgctagag atccgcggcc gctaatacga ctcactatag ggagagccgc caccatgaaa
     9601 cggacagccg acggaagcga gttcgagtca ccaaagaaga agcggaaagt cgacaagaag
     9661 tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag
     9721 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
     9781 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
     9841 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
     9901 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
     9961 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
    10021 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
    10081 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
    10141 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
    10201 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
    10261 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat
    10321 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg
    10381 agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga tgccaaactg
    10441 cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca gatcggcgac
    10501 cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct gctgagcgac
    10561 atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat gatcaagaga
    10621 tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca gcagctgcct
    10681 gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg ctacattgac
    10741 ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga aaagatggac
    10801 ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa gcagcggacc
    10861 ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc cattctgcgg
    10921 cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga gaagatcctg
    10981 accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag attcgcctgg
    11041 atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt ggtggacaag
    11101 ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa cctgcccaac
    11161 gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta taacgagctg
    11221 accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag cggcgagcag
    11281 aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt gaagcagctg
    11341 aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc cggcgtggaa
    11401 gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat caaggacaag
    11461 gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct gaccctgaca
    11521 ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca cctgttcgac
    11581 gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag gctgagccgg
    11641 aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga tttcctgaag
    11701 tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag cctgaccttt
    11761 aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca cgagcacatt
    11821 gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt gaaggtggtg
    11881 gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat cgaaatggcc
    11941 agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat gaagcggatc
    12001 gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt ggaaaacacc
    12061 cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga tatgtacgtg
    12121 gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggacgctat cgtgcctcag
    12181 agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga caagaaccgg
    12241 ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa ctactggcgg
    12301 cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac caaggccgag
    12361 agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct ggtggaaacc
    12421 cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac taagtacgac
    12481 gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa gctggtgtcc
    12541 gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta ccaccacgcc
    12601 cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta ccctaagctg
    12661 gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat gatcgccaag
    12721 agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa catcatgaac
    12781 tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc tctgatcgag
    12841 acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc caccgtgcgg
    12901 aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca gacaggcggc
    12961 ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc cagaaagaag
    13021 gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta ttctgtgctg
    13081 gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa agagctgctg
    13141 gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt tctggaagcc
    13201 aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta ctccctgttc
    13261 gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca gaagggaaac
    13321 gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca ctatgagaag
    13381 ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca gcacaagcac
    13441 tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat cctggccgac
    13501 gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc catcagagag
    13561 caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc tgccgccttc
    13621 aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga ggtgctggac
    13681 gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga cctgtctcag
    13741 ctgggaggtg actctggagg atctagcgga ggatcctctg gcagcgagac accaggaaca
    13801 agcgagtcag caacaccaga gagcagtggc ggcagcagcg gcggcagcag caccctaaat
    13861 atagaagatg agtatcggct acatgagacc tcaaaagagc cagatgtttc tctagggtcc
    13921 acatggctgt ctgattttcc tcaggcctgg gcggaaaccg ggggcatggg actggcagtt
    13981 cgccaagctc ctctgatcat acctctgaaa gcaacctcta cccccgtgtc cataaaacaa
    14041 taccccatgt cacaagaagc cagactgggg atcaagcccc acatacagag actgttggac
    14101 cagggaatac tggtaccctg ccagtccccc tggaacacgc ccctgctacc cgttaagaaa
    14161 ccagggacta atgattatag gcctgtccag gatctgagag aagtcaacaa gcgggtggaa
    14221 gacatccacc ccaccgtgcc caacccttac aacctcttga gcgggctccc accgtcccac
    14281 cagtggtaca ctgtgcttga tttaaaggat gcctttttct gcctgagact ccaccccacc
    14341 agtcagcctc tcttcgcctt tgagtggaga gatccagaga tgggaatctc aggacaattg
    14401 acctggacca gactcccaca gggtttcaaa aacagtccca ccctgtttaa tgaggcactg
    14461 cacagagacc tagcagactt ccggatccag cacccagact tgatcctgct acagtacgtg
    14521 gatgacttac tgctggccgc cacttctgag ctagactgcc aacaaggtac tcgggccctg
    14581 ttacaaaccc tagggaacct cgggtatcgg gcctcggcca agaaagccca aatttgccag
    14641 aaacaggtca agtatctggg gtatcttcta aaagagggtc agagatggct gactgaggcc
    14701 agaaaagaga ctgtgatggg gcagcctact ccgaagaccc ctcgacaact aagggagttc
    14761 ctagggaagg caggcttctg tcgcctcttc atccctgggt ttgcagaaat ggcagccccc
    14821 ctgtaccctc tcaccaaacc ggggactctg tttaattggg gcccagacca acaaaaggcc
    14881 tatcaagaaa tcaagcaagc tcttctaact gccccagccc tggggttgcc agatttgact
    14941 aagccctttg aactctttgt cgacgagaag cagggctacg ccaaaggtgt cctaacgcaa
    15001 aaactgggac cttggcgtcg gccggtggcc tacctgtcca aaaagctaga cccagtagca
    15061 gctgggtggc ccccttgcct acggatggta gcagccattg ccgtactgac aaaggatgca
    15121 ggcaagctaa ccatgggaca gccactagtc attctggccc cccatgcagt agaggcacta
    15181 gtcaaacaac cccccgaccg ctggctttcc aacgcccgga tgactcacta tcaggccttg
    15241 cttttggaca cggaccgggt ccagttcgga ccggtggtag ccctgaaccc ggctacgctg
    15301 ctcccactgc ctgaggaagg gctgcaacac aactgccttg atatcctggc cgaagcccac
    15361 ggaacccgac ccgacctaac ggaccagccg ctcccagacg ccgaccacac ctggtacacg
    15421 gatggaagca gtctcttaca agagggacag cgtaaggcgg gagctgcggt gaccaccgag
    15481 accgaggtaa tctgggctaa agccctgcca gccgggacat ccgctcagcg ggctgaactg
    15541 atagcactca cccaggccct aaagatggca gaaggtaaga agctaaatgt ttatactgat
    15601 agccgttatg cttttgctac tgcccatatc catggagaaa tatacagaag gcgtgggtgg
    15661 ctcacatcag aaggcaaaga gatcaaaaat aaagacgaga tcttggccct actaaaagcc
    15721 ctctttctgc ccaaaagact tagcataatc cattgtccag gacatcaaaa gggacacagc
    15781 gccgaggcta gaggcaaccg gatggctgac caagcggccc gaaaggcagc catcacagag
    15841 actccagaca cctctaccct cctcatagaa aattcatcac cctctggcgg ctcaaaaaga
    15901 accgccgacg gcagcgaatt cgagcccaag aagaagagga aagtctaacc ggt
//
