LOCUS       MSCV-JAK3-WT_BFP        9815 bp DNA     circular SYN 23-JUL-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2023 from SnapGene 7.0.1
            https://www.snapgene.com
COMMENT     RE used for cloning: XhoI + EcoRI
FEATURES             Location/Qualifiers
     source          1..9815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(342..361)
                     /locus_tag="2057"
                     /PCR_conditions="primer sequence:CCTACAGGTGGGGTCTTTCA"
                     /label=2057
                     /note="Match: CCTACAGGTGGGGTCTTTCA&lt;br&gt;Length of
                     match: 20&lt;br&gt;Accuracy (%): 100&lt;br&gt;Description: 
                     reverse primer in MSCV-GFP&lt;br&gt;Found at strand: 
                     Negative"
     primer_bind     844..854
                     /locus_tag="2057(1)"
                     /PCR_conditions="primer sequence:CCTACAGGTGGGGTCTTTCA"
                     /label=2057
                     /label=2057(1)
                     /note="Match: CCTACAGGTGGGGTCTTTCA&lt;br&gt;Length of
                     match: 20&lt;br&gt;Accuracy (%): 100&lt;br&gt;Description: 
                     reverse primer in MSCV-GFP&lt;br&gt;Found at strand: 
                     Negative"
     primer_bind     1700..1719
                     /locus_tag="1000_MSCV seq primer"
                     /PCR_conditions="primer sequence:CCTCGATCCTCCCTTTATCC"
                     /label=1000_MSCV seq primer
                     /note="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"
     CDS             1796..5170
                     /codon_start=1
                     /locus_tag="JAK3 M511I"
                     /label=JAK3
                     /label=JAK3 M511I
                     /translation="MAPPSEETPLIPQRSCSLLSTEAGALHVLLPARGPGPPQRLSFSF
                     GDHLAEDLCVQAAKASGILPVYHSLFALATEDLSCWFPPSHIFSVEDASTQVLLYRIRF
                     YFPNWFGLEKCHRFGLRKDLASAILDLPVLEHLFAQHRSDLVSGRLPVGLSLKEQGECL
                     SLAVLDLARMAREQAQRPGELLKTVSYKACLPPSLRDLIQGLSFVTRRRIRRTVRRALR
                     RVAACQADRHSLMAKYIMDLERLDPAGAAETFHVGLPGALGGHDGLGLLRVAGDGGIAW
                     TQGEQEVLQPFCDFPEIVDISIKQAPRVGPAGEHRLVTVTRTDNQILEAEFPGLPEALS
                     FVALVDGYFRLTTDSQHFFCKEVAPPRLLEEVAEQCHGPITLDFAINKLKTGGSRPGSY
                     VLRRSPQDFDSFLLTVCVQNPLGPDYKGCLIRRSPTGTFLLVGLSRPHSSLRELLATCW
                     DGGLHVDGVAVTLTSCCIPRPKEKSNLIVVQRGHSPPTSSLVQPQSQYQLSQMTFHKIP
                     ADSLEWHENLGHGSFTKIYRGCRHEVVDGEARKTEVLLKVMDAKHKNCMESFLEAASLM
                     SQVSYRHLVLLHGVCMAGDSTMVQEFVHLGAIDMYLRKRGHLVPASWKLQVVKQLAYAL
                     NYLEDKGLPHGNVSARKVLLAREGADGSPPFIKLSDPGVSPAVLSLEMLTDRIPWVAPE
                     CLREAQTLSLEADKWGFGATVWEVFSGVTMPISALDPAKKLQFYEDRQQLPAPKWTELA
                     LLIQQCMAYEPVQRPSFRAVIRDLNSLISSDYELLSDPTPGALAPRDGLWNGAQLYACQ
                     DPTIFEERHLKYISQLGKGNFGSVELCRYDPLGDNTGALVAVKQLQHSGPDQQRDFQRE
                     IQILKALHSDFIVKYRGVSYGPGRQSLRLVMEYLPSGCLRDFLQRHRARLDASRLLLYS
                     SQICKGMEYLGSRRCVHRDLAARNILVESEAHVKIADFGLAKLLPLDKDYYVVREPGQS
                     PIFWYAPESLSDNIFSRQSDVWSFGVVLYELFTYCDKSCSPSAEFLRMMGCERDVPALC
                     RLLELLEEGQRLPAPPACPAEVHELMKLCWAPSPQDRPSFSALGPQLDMLWSGSRGCET
                     HAFTAHPEGKHHSLSFS"
     primer_bind     2752..2772
                     /locus_tag="1170_JAK3 FWD seq primer"
                     /label=1170_JAK3 FWD seq primer
     primer_bind     complement(4325..4344)
                     /locus_tag="1171_JAK3 Rev seq primer"
                     /PCR_conditions="primer sequence:GCACCTGTATTGTCGCCTAG"
                     /label=1171_JAK3 Rev seq primer
                     /note="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;"
     misc_feature    complement(5211..5229)
                     /locus_tag="6285"
                     /label=6285
     enhancer        5244..5744
                     /locus_tag="IRES"
                     /label=IRES
     primer_bind     complement(5311..5330)
                     /locus_tag="FGL 1001"
                     /PCR_conditions="primer sequence:AGACCCCTAGGAATGCTCGT"
                     /label=FGL 1001
                     /note="&lt;html&gt;&lt;body&gt;Match: 
                     AGACCCCTAGGAATGCTCGT&lt;br/&gt;Length of match: 
                     20&lt;br/&gt;Accuracy (%): 100&lt;br/&gt;Description: 
                     reverse primer in MSCV-GFP vector (140 bp downstream of 
                     EcoRI)&lt;br/&gt;Found at strand: 
                     Negative&lt;/body&gt;&lt;/html&gt;"
     primer_bind     complement(5480..5504)
                     /locus_tag="2821-R"
                     /PCR_conditions="primer
                     sequence:GCAGGTGTATCTTATACACGTGGCT"
                     /label=2821-R
     primer_bind     5628..5647
                     /locus_tag="2814 - fw"
                     /PCR_conditions="primer sequence:ATCTGATCTGGGGCCTCGGT"
                     /label=2814 - fw
     CDS             5755..6485
                     /locus_tag="Insert"
                     /label=MTAGBFP2
                     /label=Insert
                     /note="/origin=PCR from MTAGBFP2 for MSCV-GFP (753bp 
                     linear)
                     Fragment: from BbsI cut at 13^14 to SalI cut at 744^745, 
                     731bp           Orientation: original           Plus strand
                     5':   no change           Plus strand 3':   no change      
                         Minus strand 5':  no change
                     Minus strand 3':  no change           "
     primer_bind     complement(6552..6571)
                     /locus_tag="2057(2)"
                     /PCR_conditions="primer sequence:CCTACAGGTGGGGTCTTTCA"
                     /label=2057
                     /label=2057(2)
                     /note="Match: CCTACAGGTGGGGTCTTTCA&lt;br&gt;Length of
                     match: 20&lt;br&gt;Accuracy (%): 100&lt;br&gt;Description: 
                     reverse primer in MSCV-GFP&lt;br&gt;Found at strand: 
                     Negative"
     primer_bind     7049..7064
                     /locus_tag="2057(3)"
                     /PCR_conditions="primer sequence:CCTACAGGTGGGGTCTTTCA"
                     /label=2057
                     /label=2057(3)
                     /note="Match: CCTACAGGTGGGGTCTTTCA&lt;br&gt;Length of
                     match: 20&lt;br&gt;Accuracy (%): 100&lt;br&gt;Description: 
                     reverse primer in MSCV-GFP&lt;br&gt;Found at strand: 
                     Negative"
     primer_bind     complement(7206..7225)
                     /locus_tag="2055"
                     /PCR_conditions="primer sequence:TTCACAATTCTCCCCAGCTC"
                     /label=2055
                     /note="Match: TTCACAATTCTCCCCAGCTC&lt;br&gt;Length of
                     match: 20&lt;br&gt;Accuracy (%): 100&lt;br&gt;Description: 
                     reverse primer in MSCV-GFP&lt;br&gt;Found at strand: 
                     Negative"
     gene            7546..8219
                     /locus_tag="Ori"
                     /label=Ori
     misc_feature    complement(8364..9224)
                     /locus_tag="Amp"
                     /label=Amp
     primer_bind     9783..9802
                     /locus_tag="2056"
                     /PCR_conditions="primer sequence:GTTTTCCCAGTCACGACGTT"
                     /label=2056
                     /note="Match: GTTTTCCCAGTCACGACGTT&lt;br&gt;Length of
                     match: 20&lt;br&gt;Accuracy (%): 100&lt;br&gt;Description: 
                     forward primer in MSCV-GFP&lt;br&gt;Found at strand: 
                     Positive"
ORIGIN
        1 gccagtatgg tgcatgcagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
       61 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
      121 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
      181 gatgcgtccg gcgtagaggc gattagtcca atttgttaaa gacaggatat cagtggtcca
      241 ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat
      301 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
      361 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga
      421 atagagaagt tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga
      481 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
      541 ggtcccgccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
      601 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
      661 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc
      721 ctccgataga ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt gctgtttgca
      781 tccgaatcgt ggactcgctg atccttggga gggtctcctc agattgattg actgcccacc
      841 tcgggggtct ttcatttgga ggttccaccg agatttggag acccctgccc agggaccacc
      901 gacccccccg ccgggaggta agctggccag cggtcgtttc gtgtctgtct ctgtctttgt
      961 gcgtgtttgt gccggcatct aatgtttgcg cctgcgtctg tactagttag ctaactagct
     1021 ctgtatctgg cggacccgtg gtggaactga cgagttctga acacccggcc gcaaccctgg
     1081 gagacgtccc agggactttg ggggccgttt ttgtggcccg acctgaggaa gggagtcgat
     1141 gtggaatccg accccgtcag gatatgtggt tctggtagga gacgagaacc taaaacagtt
     1201 cccgcctccg tctgaatttt tgctttcggt ttggaaccga agccgcgcgt cttgtctgct
     1261 gcagcgctgc agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga
     1321 aaattagggc cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt
     1381 cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg
     1441 ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga
     1501 cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca
     1561 ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc
     1621 ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga
     1681 acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg
     1741 cgccggaatt agatctctcg agccagggtc cctgcccgct aggcaagttg cactcatggc
     1801 acctccaagt gaagagacgc ccctgatccc tcagcgttca tgcagcctct tgtccacgga
     1861 ggctggtgcc ctgcatgtgc tgctgcccgc tcggggcccc gggccccccc agcgcctatc
     1921 tttctccttt ggggaccact tggctgagga cctgtgcgtg caggctgcca aggccagcgg
     1981 catcctgcct gtgtaccact ccctctttgc tctggccacg gaggacctgt cctgctggtt
     2041 ccccccgagc cacatcttct ccgtggagga tgccagcacc caagtcctgc tgtacaggat
     2101 tcgcttttac ttccccaatt ggtttgggct ggagaagtgc caccgcttcg ggctacgcaa
     2161 ggatttggcc agtgctatcc ttgacctgcc agtcctggag cacctctttg cccagcaccg
     2221 cagtgacctg gtgagtgggc gcctccccgt gggcctcagt ctcaaggagc agggtgagtg
     2281 tctcagcctg gccgtgttgg acctggcccg gatggcgcga gagcaggccc agcggccggg
     2341 agagctgctg aagactgtca gctacaaggc ctgcctaccc ccaagcctgc gcgacctgat
     2401 ccagggcctg agcttcgtga cgcggaggcg tattcggagg acggtgcgca gagccctgcg
     2461 ccgcgtggcc gcctgccagg cagaccggca ctcgctcatg gccaagtaca tcatggacct
     2521 ggagcggctg gatccagccg gggccgccga gaccttccac gtgggcctcc ctggggccct
     2581 tggtggccac gacgggctgg ggctgctccg cgtggctggt gacggcggca tcgcctggac
     2641 ccagggagaa caggaggtcc tccagccctt ctgcgacttt ccagaaatcg tagacattag
     2701 catcaagcag gccccgcgcg ttggcccggc cggagagcac cgcctggtca ctgttaccag
     2761 gacagacaac cagattttag aggccgagtt cccagggctg cccgaggctc tgtcgttcgt
     2821 ggcgctcgtg gacggctact tccggctgac cacggactcc cagcacttct tctgcaagga
     2881 ggtggcaccg ccgaggctgc tggaggaagt ggccgagcag tgccacggcc ccatcactct
     2941 ggactttgcc atcaacaagc tcaagactgg gggctcacgt cctggctcct atgttctccg
     3001 ccgcagcccc caggactttg acagcttcct cctcactgtc tgtgtccaga acccccttgg
     3061 tcctgattat aagggctgcc tcatccggcg cagccccaca ggaaccttcc ttctggttgg
     3121 cctcagccga ccccacagca gtcttcgaga gctcctggca acctgctggg atggggggct
     3181 gcacgtagat ggggtggcag tgaccctcac ttcctgctgt atccccagac ccaaagaaaa
     3241 gtccaacctg atcgtggtcc agagaggtca cagcccaccc acatcatcct tggttcagcc
     3301 ccaatcccaa taccagctga gtcagatgac atttcacaag atccctgctg acagcctgga
     3361 gtggcatgag aacctgggcc atgggtcctt caccaagatt taccggggct gtcgccatga
     3421 ggtggtggat ggggaggccc gaaagacaga ggtgctgctg aaggtcatgg atgccaagca
     3481 caagaactgc atggagtcat tcctggaagc agcgagcttg atgagccaag tgtcgtaccg
     3541 gcatctcgtg ctgctccacg gcgtgtgcat ggctggagac agcaccatgg tgcaggaatt
     3601 tgtacacctg ggggccatag acatgtatct gcgaaaacgt ggccacctgg tgccagccag
     3661 ctggaagctg caggtggtca aacagctggc ctacgccctc aactatctgg aggacaaagg
     3721 cctgccccat ggcaatgtct ctgcccggaa ggtgctcctg gctcgggagg gggctgatgg
     3781 gagcccgccc ttcatcaagc tgagtgaccc tggggtcagc cccgctgtgt taagcctgga
     3841 gatgctcacc gacaggatcc cctgggtggc ccccgagtgt ctccgggagg cgcagacact
     3901 tagcttggaa gctgacaagt ggggcttcgg cgccacggtc tgggaagtgt ttagtggcgt
     3961 caccatgccc atcagtgccc tggatcctgc taagaaactc caattttatg aggaccggca
     4021 gcagctgccg gcccccaagt ggacagagct ggccctgctg attcaacagt gcatggccta
     4081 tgagccggtc cagaggccct ccttccgagc cgtcattcgt gacctcaata gcctcatctc
     4141 ttcagactat gagctcctct cagaccccac acctggtgcc ctggcacctc gtgatgggct
     4201 gtggaatggt gcccagctct atgcctgcca agaccccacg atcttcgagg agagacacct
     4261 caagtacatc tcacagctgg gcaagggcaa ctttggcagc gtggagctgt gccgctatga
     4321 cccgctaggc gacaatacag gtgccctggt ggccgtgaaa cagctgcagc acagcgggcc
     4381 agaccagcag agggactttc agcgggagat tcagatcctc aaagcactgc acagtgattt
     4441 cattgtcaag tatcgtggtg tcagctatgg cccgggccgc cagagcctgc ggctggtcat
     4501 ggagtacctg cccagcggct gcttgcgcga cttcctgcag cggcaccgcg cgcgcctcga
     4561 tgccagccgc ctccttctct attcctcgca aatctgcaag ggcatggagt acctgggctc
     4621 ccgccgctgc gtgcaccgcg acctggccgc ccgaaacatc ctcgtggaga gcgaggcaca
     4681 cgtcaagatc gctgacttcg gcctagctaa gctgctgccg cttgacaaag actactacgt
     4741 ggtccgcgag ccaggccaga gccccatttt ctggtatgcc cccgaatccc tctcggacaa
     4801 catcttctct cgccagtcag acgtctggag cttcggggtc gtcctgtacg agctcttcac
     4861 ctactgcgac aaaagctgca gcccctcggc cgagttcctg cggatgatgg gatgtgagcg
     4921 ggatgtcccc gccctctgcc gcctcttgga actgctggag gagggccaga ggctgccggc
     4981 gcctcctgcc tgccctgctg aggttcacga gctcatgaag ctgtgctggg cccctagccc
     5041 acaggaccgg ccatcattca gcgccctggg cccccagctg gacatgctgt ggagcggaag
     5101 ccgggggtgt gagactcatg ccttcactgc tcacccagag ggcaaacacc actccctgtc
     5161 cttttcatag gaattccgcc ccccccccct aacgttactg gccgaagccg cttggaataa
     5221 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     5281 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     5341 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     5401 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     5461 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     5521 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     5581 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     5641 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     5701 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatgag
     5761 cgagctgatt aaggagaaca tgatgagcga gctgattaag gagaacatgc acatgaagct
     5821 gtacatggag ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa
     5881 gccctacgag ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt
     5941 cgccttcgac atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac
     6001 ccagggcatc cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt
     6061 caccacatac gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg
     6121 ctgcctcatc tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat
     6181 gcagaagaaa acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg
     6241 cctggaaggc agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa
     6301 cgccaagacc acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta
     6361 ctatgtggac tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca
     6421 gcacgaggtg gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa
     6481 ttaagtcgac ctgcagccaa gcttatcgat aaaataaaag attttattta gtctccagaa
     6541 aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt
     6601 ttgcaaggca tggaaaatac ataactgaga atagagaagt tcagatcaag gttaggaaca
     6661 gagagacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     6721 cagggccaag aacagatggt ccccagatgc ggtcccgccc tcagcagttt ctagagaacc
     6781 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa
     6841 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga
     6901 gcccacaacc cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg
     6961 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag
     7021 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatgggta acagtttctt
     7081 gaagttggag aacaacattc tgagggtagg agtcgaatat taagtaatcc tgactcaatt
     7141 agccactgtt ttgaatccac atactccaat actcctgaaa tagttcatta tggacagcgc
     7201 agaaagagct ggggagaatt gtgaaattgt tatccgctca caattccaca caacatacga
     7261 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     7321 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7381 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     7441 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     7501 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     7561 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     7621 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     7681 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     7741 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     7801 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     7861 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     7921 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     7981 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     8041 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     8101 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     8161 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     8221 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     8281 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     8341 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     8401 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     8461 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     8521 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     8581 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     8641 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     8701 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     8761 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     8821 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     8881 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     8941 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     9001 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     9061 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     9121 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     9181 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     9241 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     9301 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     9361 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     9421 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     9481 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     9541 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     9601 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     9661 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     9721 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     9781 gggttttccc agtcacgacg ttgtaaaacg acggc
//
