LOCUS       File_S1       10316 bp DNA     circular SYN 15-JUN-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    pUAST-2X attB
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10316)
  AUTHORS   Alex Teague
  TITLE     Direct Submission
  JOURNAL   Exported Jul 23, 2023 from SnapGene Viewer 7.0.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        623..642
                     /label=T7 promoter
                     /note="T7 promoter"
                     /note="color: #ffc0cb"
     misc_feature    667..4802
                     /label=white dominant eye marker
                     /note="white dominant eye marker"
                     /note="This feature has 2 segments:
                        1:  667 .. 3883 / #ff2430
                        2: 3884 .. 4802 / #ff2430"
     misc_feature    4860..5559
                     /label=SV40 poly(A)
                     /label=SV40 poly(A)(2)
                     /note="SV40 poly(A)"
                     /note="color: #7dff74"
     misc_feature    5566..5929
                     /label=5xUAS-hsp70 basal promoter
                     /label=5xUAS-hsp70
                     /note="5xUAS-hsp70"
                     /note="color: #7cf2ff"
     misc_feature    5581..5599
                     /label=UAS repeat
                     /note="color: #a5c1ff"
     misc_feature    5600..5618
                     /label=UAS repeat
                     /label=UAS repeat(3)
                     /note="color: #4dffa4"
     misc_feature    5619..5637
                     /label=UAS repeat
                     /label=UAS repeat(1)
                     /note="color: #ff5ea9"
     misc_feature    5638..5656
                     /label=UAS repeat
                     /label=UAS repeat(5)
                     /note="color: #ff6cf3"
     misc_feature    5657..5673
                     /label=partial UAS repeat
                     /note="color: #b33f8f"
     misc_feature    5930..5975
                     /label=MCS2
                     /label=multiple cloning site
                     /note="multiple cloning site"
                     /note="color: #ffc0cb"
     misc_feature    5988..6687
                     /label=SV40 poly(A)
                     /label=SV40 poly(A)(1)
                     /note="SV40 poly(A)"
                     /note="color: #7dff74"
     misc_feature    6694..7057
                     /label=5xUAS-hsp70 basal promoter
                     /label=5xUAS-hsp70(1)
                     /note="5xUAS-hsp70"
                     /note="color: #7cf2ff"
     misc_feature    6709..6727
                     /label=UAS repeat
                     /label=UAS repeat(2)
                     /note="color: #a5c1ff"
     misc_feature    6728..6746
                     /label=UAS repeat
                     /label=UAS repeat(9)
                     /note="color: #4dffa4"
     misc_feature    6747..6765
                     /label=UAS repeat
                     /label=UAS repeat(6)
                     /note="color: #ff5ea9"
     misc_feature    6766..6784
                     /label=UAS repeat
                     /label=UAS repeat(11)
                     /note="color: #ff6cf3"
     misc_feature    6785..6801
                     /label=partial UAS repeat
                     /label=partial UAS repeat(1)
                     /note="color: #b33f8f"
     misc_feature    7058..7112
                     /label=MCS
                     /label=multiple cloning site(3)
                     /note="multiple cloning site"
                     /note="color: #ffc0cb"
     misc_feature    7113..7812
                     /label=SV40 poly(A)
                     /note="SV40 poly(A)"
                     /note="color: #7dff74"
     misc_feature    7819..8103
                     /label=attB site
                     /note="attB site"
                     /note="color: #ffe84f"
     promoter        8127..8146
                     /label=T3 promoter
                     /note="T3 promoter"
                     /note="color: #ffc0cb"
     CDS             complement(join(9328..10122,10153..10188))
                     /codon_start=1
                     /product="ampicillin resistance protein"
                     /label=ampicillin resistance protein
                     /note="This feature has 2 segments:
                        1:  9328 .. 10122 / #ffc0cb
                        2: 10153 .. 10188 / #ffc0cb"
                     /db_xref="GI:124365493"
                     /protein_id="ABN09740.1"
                     /translation="MSIQHFRVALIPAHPETLVKVKDAEDQLGARVGYIELDLNSGKIL
                     ESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDG
                     MTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPN
                     DERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADK
                     SGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggtttaaac gactcctagg gactaccggt gatacttaat taactgaaga cgtcctagag
     4861 gatctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
     4921 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
     4981 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
     5041 gaatgccttt aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga
     5101 ggctactgct gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc
     5161 caaggacttt ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac
     5221 tcttgcttgc tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat
     5281 tatggaaaaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     5341 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     5401 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     5461 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5521 tgtccaaact catcaatgta tcttatcatg tctggatcca agcttctgca ggtcggagta
     5581 ctgtcctccg agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
     5641 tcctccgagc ggagtactgt cctccgagcg gagactctag cgagcgccgg agtataaata
     5701 gaggcgcttc gtctacggag cgacaattca attcaaacaa gcaaagtgaa cacgtcgcta
     5761 agcgaaagct aagcaaataa acaagcgcag ctgaacaagc taaacaatct gcagtaaagt
     5821 gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac tgcaactact
     5881 gaaatctgcc aagaagtaat tattgaatac aagaagagaa ctctgaatag ggaattggga
     5941 attggcgcgc catatgcgta cggatacgcg atcgctttaa acctagagga tctttgtgaa
     6001 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     6061 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     6121 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     6181 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     6241 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     6301 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     6361 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     6421 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     6481 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     6541 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6601 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6661 tcaatgtatc ttatcatgtc tggatccgca tgcctgcagg tcggagtact gtcctccgag
     6721 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
     6781 agtactgtcc tccgagcgga gactctagcg agcgccggag tataaataga ggcgcttcgt
     6841 ctacggagcg acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa
     6901 gcaaataaac aagcgcagct gaacaagcta aacaatctgc agtaaagtgc aagttaaagt
     6961 gaatcaatta aaagtaacca gcaaccaagt aaatcaactg caactactga aatctgccaa
     7021 gaagtaatta ttgaatacaa gaagagaact ctgaataggg aattgggaat tcgttaacag
     7081 atctgcggcc gcggctcgag ggtacctcta gaggatcttt gtgaaggaac cttacttctg
     7141 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     7201 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     7261 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     7321 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     7381 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
     7441 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
     7501 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga tgtatagtgc
     7561 cttgactaga gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     7621 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     7681 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7741 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     7801 atgtctggat ccactagtgt cgacgatgta ggtcacggtc tcgaagccgc ggtgcgggtg
     7861 ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct ggtccatcat
     7921 gatgaacggg tcgaggtggc ggtagttgat cccggcgaac gcgcggcgca ccgggaagcc
     7981 ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc acgggacgtg cgacggcgtc
     8041 ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg acggtcacgg cgggcatgtc
     8101 gacactagtt ctagccagct tttgttccct ttagtgaggg ttaatttcga gcttggcgta
     8161 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8221 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8281 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8341 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8401 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8461 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8521 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8581 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8641 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8701 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8761 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8821 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8881 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8941 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9001 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9061 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9121 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9181 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9241 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9301 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9361 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9421 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9481 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9541 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9601 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9661 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9721 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9781 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9841 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9901 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9961 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10021 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10081 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10141 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10201 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10261 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
