LOCUS       pDA010.188              7092 bp ds-DNA     circular     18-NOV-2021
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: P28a-MscL-eGFP.str
            From plasmid pBEST-Ptar-UTR1-MotA-eGFP, insert MscL gene (no stop)
            between Acc65I-ApaI. ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     CDS             complement(451..1110)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1657..1676
                     /label="Seq6-s"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1789..1830)
                     /label="oBT079pBestlinR"
                     /note="sequence: tggcacttttcggGCTAAGCTTGGCCGGGCTCAGCACGTTAC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1808..1814
                     /label="Ptar promoter"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          complement(1818..1850)
                     /label="oJF148b"
                     /note="sequence: GACGTCGGAATTGCCAcaggtggcacttttcgg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1828..1845)
                     /label="oBT022_143.J3linR"
                     /note="sequence: CGGAATTGCCACAGGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1835..1850)
                     /label="Copy of oRC002"
                     /note="sequence: GACGTCGGAATTGCCA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1835..1850)
                     /label="oJF148"
                     /note="sequence: GACGTCGGAATTGCCA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1853..1877)
                     /label="oRC096"
                     /note="sequence: tcatgataataatggtttcttaCCC"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1856..1959
                     /label="Conserve in ColE1"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1936..2000
                     /label="60bp_R"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1945..1959
                     /label="overhang"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1946..2005
                     /label="dCas9-113-F"
                     /note="sequence: aaggatgatttctgATGCATctgatggctagctcagtcctagggattatgctagcGAATT"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1960..1965
                     /label="Mph1103I"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1966..2000
                     /label="Bba_J23113"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1967..1983
                     /label="oRC013"
                     /note="sequence: GGCAATTCCGACGTCtttacggctagctcagcc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1981..2031
                     /label="oRC020"
                     /note="sequence: gtcctagggattatgctagcGAATTCATTAAAGAGGAGAAAATGCATatgg"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2001..2027
                     /label="Bujard RBS (strong)"
                     /ApEinfo_revcolor="#db7aff"
                     /ApEinfo_fwdcolor="#db7aff"
     misc_feature    2001..2056
                     /label="60bp_F"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2003..2027)
                     /label="Copy of oRC006"
                     /note="sequence: ATGCATTTTCTCCTCTTTAATGAAT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     RBS             2010..2021
                     /label="RBS (strong)"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_fwdcolor="#996633"
     misc_feature    2022..2027
                     /label="Mph1103I"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2028..2042
                     /label="overhang"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    2028..2056
                     /label="Annealing"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2028..2056
                     /label="Copy of oRC005"
                     /note="sequence: ATGGATAAGAAATACTCAATAGGCTTAGc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2028..6131
                     /label="dCas9"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             2028..6131
                     /label="Translation 2028-6131"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
     primer          4863..4884
                     /label="Copy of oRC008"
                     /note="sequence: GAGGAGAAAATGCATatggaacaaaaacttattagcgaagaagatcttGgt"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          5941..5971
                     /label="oRC083"
                     /note="sequence: AAGCAGAAAATATTATTCATTTATTTACGTT"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    5941..6131
                     /label="fRC062"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          6100..6120
                     /label="oCD090"
                     /note="sequence: CACGCATTGATTTGAGTCAGC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(6110..6131)
                     /label="oRC084"
                     /note="sequence: GTCACCTCCTAGCTGACTCAAA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          6135..6158
                     /label="oBT127_SynthT_F"
                     /note="sequence: aacgcatgagAAAGCCCCCGGAAG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          6135..6161
                     /label="oBT152_009linFt"
                     /note="sequence: AACGCATGAGAAAGCCCCCGGAAGATC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     terminator      6135..6187
                     /label="ECK120033736 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(6171..6202)
                     /label="oRC019"
                     /note="sequence: ACGGAGTAAGGATCCgcgcaataaaAAAGCCC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          6188..6207
                     /label="Copy of oRC001"
                     /note="sequence: GGATCCTTACTCCGTCGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          6338..6355
                     /label="oRC019"
                     /note="sequence: ACGGAGTAAGGATCCgcgcaataaaAAAGCCC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          6455..6472
                     /label="oRC097"
                     /note="sequence: CTTCCGAATTGATCCGTC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    complement(6603..6620)
                     /label="Seq7-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(6655..6674)
                     /label="Seq8-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(6763..353)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
ORIGIN
        1 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG
       61 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA
      121 CACTAGAAGG ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG
      181 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG
      241 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC
      301 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC
      361 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG
      421 TATATATGAG TAAACTTGGT CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC
      481 AGCGATCTGT CTATTTCGTT CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC
      541 GATACGGGAG GGCTTACCAT CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC
      601 ACCGGCTCCA GATTTATCAG CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG
      661 TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG
      721 TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA TCGTGGTGTC
      781 ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA GGCGAGTTAC
      841 ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG
      901 AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA ATTCTCTTAC
      961 TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA AGTCATTCTG
     1021 AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG ATAATACCGC
     1081 GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG GGCGAAAACT
     1141 CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG CACCCAACTG
     1201 ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG GAAGGCAAAA
     1261 TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC TCTTCCTTTT
     1321 TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG
     1381 TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA
     1441 CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA TCACGAGGCC
     1501 CTTTCGTCTT CAAGAATTCT GGCGAATCCT CTGACCAGCC AGAAAACGAC CTTTCTGTGG
     1561 TGAAACCGGA TGCTGCAATT CAGAGCGGCA GCAAGTGGGG GACAGCAGAA GACCTGACCG
     1621 CCGCAGAGTG GATGTTTGAC ATGGTGAAGA CTATCGCACC ATCAGCCAGA AAACCGAATT
     1681 TTGCTGGGTG GGCTAACGAT ATCCGCCTGA TGCGTGAACG TGACGGACGT AACCACCGCG
     1741 ACATGTGTGT GCTGTTCCGC TGGGCATGCC AGGACAACTT CTGGTCCGGT AACGTGCTGA
     1801 GCCCGGCCAA GCTTAGCccg aaaagtgcca cctgTGGCAA TTCCGACGTC CCGGGtaaga
     1861 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc agaatttcag
     1921 ataaaaaaaa tccttagctt tcgctaagga tgatttctgA TGCATctgat ggctagctca
     1981 gtcctaggga ttatgctagc GAATTCATTA AAGAGGAGAA AATGCATATG GATAAGAAAT
     2041 ACTCAATAGG CTTAGcTATC GGCACAAATA GCGTCGGATG GGCGGTGATC ACTGATGAAT
     2101 ATAAGGTTCC GTCTAAAAAG TTCAAGGTTC TGGGAAATAC AGACCGCCAC AGTATCAAAA
     2161 AAAATCTTAT AGGGGCTCTT TTATTTGACA GTGGAGAGAC AGCGGAAGCG ACTCGTCTCA
     2221 AACGGACAGC TCGTAGAAGG TATACACGTC GGAAGAATCG TATTTGTTAT CTACAGGAGA
     2281 TTTTTTCAAA TGAGATGGCG AAAGTAGATG ATAGTTTCTT TCATCGACTT GAAGAGTCTT
     2341 TTTTGGTGGA AGAAGACAAG AAGCATGAAC GTCATCCTAT TTTTGGAAAT ATAGTAGATG
     2401 AAGTTGCTTA TCATGAGAAA TATCCAACTA TCTATCATCT GCGAAAAAAA TTGGTAGATT
     2461 CTACTGATAA AGCGGATTTG CGCTTAATCT ATTTGGCCTT AGCGCATATG ATTAAGTTTC
     2521 GTGGTCATTT TTTGATTGAG GGAGATTTAA ATCCTGATAA TAGTGATGTG GACAAACTAT
     2581 TTATCCAGTT GGTACAAACC TACAATCAAT TATTTGAAGA AAACCCTATT AACGCAAGTG
     2641 GAGTAGATGC TAAAGCGATT CTTTCTGCAC GATTGAGTAA ATCAAGACGA TTAGAAAATC
     2701 TCATTGCTCA GCTCCCCGGT GAGAAGAAAA ATGGCTTATT TGGGAATCTC ATTGCTTTGT
     2761 CATTGGGTTT GACCCCTAAT TTTAAATCAA ATTTTGATTT GGCAGAAGAT GCTAAATTAC
     2821 AGCTTTCAAA AGATACTTAC GATGATGATT TAGATAATTT ATTGGCGCAA ATTGGAGATC
     2881 AATATGCTGA TTTGTTTTTG GCAGCTAAGA ATTTATCAGA TGCTATTTTA CTTTCAGATA
     2941 TCCTAAGAGT AAATACTGAA ATAACTAAGG CTCCCCTATC AGCTTCAATG ATTAAACGCT
     3001 ACGATGAACA TCATCAAGAC TTGACTCTTT TAAAAGCTTT AGTTCGACAA CAACTTCCAG
     3061 AAAAGTATAA AGAAATCTTT TTTGATCAAT CAAAAAACGG ATATGCAGGT TATATTGATG
     3121 GGGGAGCTAG CCAAGAAGAA TTTTATAAAT TTATCAAACC AATTTTAGAA AAAATGGATG
     3181 GTACTGAGGA ATTATTGGTG AAACTAAATC GTGAAGATTT GCTGCGCAAG CAACGGACCT
     3241 TTGACAACGG CTCTATTCCC CATCAAATTC ACTTGGGTGA GCTGCATGCT ATTTTGAGAA
     3301 GACAAGAAGA CTTTTATCCA TTTTTAAAAG ACAATCGTGA GAAGATTGAA AAAATCTTGA
     3361 CTTTTCGAAT TCCTTATTAT GTTGGTCCAT TGGCGCGTGG CAATAGTCGT TTTGCATGGA
     3421 TGACTCGGAA GTCTGAAGAA ACAATTACCC CATGGAATTT TGAAGAAGTT GTCGATAAAG
     3481 GTGCTTCAGC TCAATCATTT ATTGAACGCA TGACAAACTT TGATAAAAAT CTTCCAAATG
     3541 AAAAAGTACT ACCAAAACAT AGTTTGCTTT ATGAGTATTT TACGGTTTAT AACGAATTGA
     3601 CAAAGGTCAA ATATGTTACT GAAGGAATGC GAAAACCAGC ATTTCTTTCA GGTGAACAGA
     3661 AGAAAGCCAT TGTTGATTTA CTCTTCAAAA CAAATCGAAA AGTAACCGTT AAGCAATTAA
     3721 AAGAAGATTA TTTCAAAAAA ATAGAATGTT TTGATAGTGT TGAAATTTCA GGAGTTGAAG
     3781 ATAGATTTAA TGCTTCATTA GGTACCTACC ATGATTTGCT AAAAATTATT AAAGATAAAG
     3841 ATTTTTTGGA TAATGAAGAA AATGAAGATA TCTTAGAGGA TATTGTTTTA ACATTGACCT
     3901 TATTTGAAGA TAGGGAGATG ATTGAGGAAA GACTTAAAAC ATATGCTCAC CTCTTTGATG
     3961 ATAAGGTGAT GAAACAGCTT AAACGTCGCC GTTATACTGG TTGGGGACGT TTGTCTCGAA
     4021 AATTGATTAA TGGTATTAGG GATAAGCAAT CTGGCAAAAC AATATTAGAT TTTTTGAAAT
     4081 CAGATGGTTT TGCCAATCGC AATTTTATGC AGCTGATCCA TGATGATAGT TTGACATTTA
     4141 AAGAAGACAT TCAAAAAGCA CAAGTGTCTG GACAAGGCGA TAGTTTACAT GAACATATTG
     4201 CAAATTTAGC TGGTAGCCCT GCTATTAAAA AAGGTATTTT ACAGACTGTA AAAGTTGTTG
     4261 ATGAATTGGT CAAAGTAATG GGGCGGCATA AGCCAGAAAA TATCGTTATT GAAATGGCAC
     4321 GTGAAAATCA GACAACTCAA AAGGGCCAGA AAAATTCGCG AGAGCGTATG AAACGAATCG
     4381 AAGAAGGTAT CAAAGAATTA GGAAGTCAGA TTCTTAAAGA GCATCCTGTT GAAAATACTC
     4441 AATTGCAAAA TGAAAAGCTC TATCTCTATT ATCTCCAAAA TGGAAGAGAC ATGTATGTGG
     4501 ACCAAGAATT AGATATTAAT CGTTTAAGTG ATTATGATGT CGATgcCATT GTTCCACAAA
     4561 GTTTCCTTAA AGACGATTCA ATAGACAATA AGGTCTTAAC GCGTTCTGAT AAAAATCGTG
     4621 GTAAATCGGA TAACGTTCCA AGTGAAGAAG TAGTCAAAAA GATGAAAAAC TATTGGAGAC
     4681 AACTTCTAAA CGCCAAGTTA ATCACTCAAC GTAAGTTTGA TAATTTAACG AAAGCTGAAC
     4741 GTGGAGGTTT GAGTGAACTT GATAAAGCTG GTTTTATCAA ACGCCAATTG GTTGAAACTC
     4801 GCCAAATCAC TAAGCATGTG GCACAAATTT TGGATAGTCG CATGAATACT AAATACGATG
     4861 AAAATGATAA ACTTATTCGA GAGGTTAAAG TGATTACCTT AAAATCTAAA TTAGTTTCTG
     4921 ACTTCCGAAA AGATTTCCAA TTCTATAAAG TACGTGAGAT TAACAATTAC CATCATGCCC
     4981 ATGATGCGTA TCTAAATGCC GTCGTTGGAA CTGCTTTGAT TAAGAAATAT CCAAAACTTG
     5041 AATCGGAGTT TGTCTATGGT GATTATAAAG TTTATGATGT TCGTAAAATG ATTGCTAAGT
     5101 CTGAGCAAGA AATAGGCAAA GCAACCGCAA AATATTTCTT TTACTCTAAT ATCATGAACT
     5161 TCTTCAAAAC AGAAATTACA CTTGCAAATG GAGAGATTCG CAAACGCCCT CTAATCGAAA
     5221 CTAATGGGGA AACTGGAGAA ATTGTCTGGG ATAAAGGGCG AGATTTTGCC ACAGTGCGCA
     5281 AAGTATTGTC CATGCCCCAA GTCAATATTG TCAAGAAAAC AGAAGTACAG ACAGGCGGAT
     5341 TCTCCAAGGA GTCAATTTTA CCAAAAAGAA ATTCGGACAA GCTTATTGCT CGTAAAAAAG
     5401 ACTGGGATCC AAAAAAATAT GGTGGTTTTG ATAGTCCAAC GGTAGCTTAT TCAGTCCTAG
     5461 TGGTTGCTAA GGTGGAAAAA GGGAAATCGA AGAAGTTAAA ATCCGTTAAA GAGTTACTAG
     5521 GGATCACAAT TATGGAAAGA AGTTCCTTTG AAAAAAATCC GATTGACTTT TTAGAAGCTA
     5581 AAGGATATAA GGAAGTTAAA AAAGACTTAA TCATTAAACT ACCTAAATAT AGTCTTTTTG
     5641 AGTTAGAAAA CGGTCGTAAA CGGATGCTGG CTAGTGCCGG AGAATTACAA AAAGGAAATG
     5701 AGCTGGCTCT GCCAAGCAAA TATGTGAATT TTTTATATTT AGCTAGTCAT TATGAAAAGT
     5761 TGAAGGGTAG TCCAGAAGAT AACGAACAAA AACAATTGTT TGTGGAGCAG CATAAGCATT
     5821 ATTTAGATGA GATTATTGAG CAAATCAGTG AATTTTCTAA GCGTGTTATT TTAGCAGATG
     5881 CCAATTTAGA TAAAGTTCTT AGTGCATATA ACAAACATAG AGACAAACCA ATACGTGAAC
     5941 AAGCAGAAAA TATTATTCAT TTATTTACGT TGACGAATCT TGGAGCTCCC GCTGCTTTTA
     6001 AATATTTTGA TACAACAATT GATCGTAAAC GATATACGTC TACAAAAGAA GTTTTAGATG
     6061 CCACTCTTAT CCATCAATCC ATCACTGGTC TTTATGAAAC ACGCATTGAT TTGAGTCAGC
     6121 TAGGAGGTGA CAACaacgca tgagAAAGCC CCCGGAAGAT CACCTTCCGG GGGCTTTttt
     6181 attgcgcGGA TCCTTACTCC GTCGACAAGC TTGCGGCCGC ACTCGAGGAA TTCGACTCAA
     6241 TTAGTTCAGT CAGTTTCAGG ATATTAGTCA TCTCTACATT GATTATGAGT ATTCAGAAAT
     6301 TCCTTAAATA TTCTGACAAA TGCTCTTTCC CTAAACTCCC CCCATAAAAA AACCCGCCGA
     6361 AGCGGGTTTT TACGTTATTT GCGGATTAAC GATTACTCGT TATCAGAACC GCCCAGACCT
     6421 GCGTTCAGCA GTTCTGCCAG GCTGGCAGAT GCGTCTTCCG AATTGATCCG TCGACCGATG
     6481 CCCTTGAGAG CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT GACTATCGTC
     6541 GCCGCACTTA TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC GGCAGCGCTC
     6601 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC
     6661 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA
     6721 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT
     6781 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG
     6841 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG
     6901 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG
     6961 CGTGGCGCTT TCTCAATGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC
     7021 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA
     7081 CTATCGTCTT GA
//