LOCUS       pRC011                  3398 bp ds-DNA     circular     16-APR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          169..189
                     /label="oBT15 pAmp seqF"
                     /note="sequence: AAGGGCGACACGGAAATGTTG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          173..194
                     /label="oCD344"
                     /note="sequence: gcgacacggaaatgttgaatac"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(173..194)
                     /label="oCK067"
                     /note="sequence: gtattcaacatttccgtgtcgc"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    547..566
                     /label="Seq6-s"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          572..597
                     /label="oBT080_009seqF"
                     /note="sequence: TGCTGGGTGGGCTAACGATATCCGCC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          603..622
                     /label="oDA081"
                     /note="sequence: CGTGAACGTGACGGACGTAA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    698..704
                     /label="Ptar promoter"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          720..759
                     /label="oCK167"
                     /note="sequence: gggtggtgaatcCCCAGATCTtttacggctagctcagccc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(725..740)
                     /label="oRC002"
                     /note="sequence: GACGTCGGAATTGCCA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    738..740
                     /label="Overhang_2"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    738..740
                     /label="Overhang_1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          741..758
                     /label="oRC036.v2"
                     /note="sequence: TGGCAATTCCGACGTCtttacggctagctcagcc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        741..775
                     /label="J23107 promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    776..802
                     /label="Bujard RBS (strong)"
                     /ApEinfo_revcolor="#db7aff"
                     /ApEinfo_fwdcolor="#db7aff"
     RBS             785..796
                     /label="RBS (strong)"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_fwdcolor="#996633"
     primer          796..836
                     /label="oCD070"
                     /note="sequence: AGGTACCatggggcccgcttctaactttactcagttcgttc"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             803..1174
                     /label="Translation 803-1174"
                     /translation="MGPASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLNMELTIPIFATNSDCELIVKAMQGLLKDGNPIPSAIAANSGIYGGGGS"
     primer          805..827
                     /label="oRC090"
                     /note="sequence: GGTGGCGGAGGTAGCggggcccgcttctaactttactc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    812..1159
                     /label="MCPdelFG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1139..1159)
                     /label="oCD015"
                     /note="sequence: GTAGATGCCGGAGTTTGCTGC"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1140..1159)
                     /label="oRC091"
                     /note="sequence: gaagcggggttttttgcggGCGGCCGCTTAgtagatgccggagtttgctg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1159..1174)
                     /label="oRC009"
                     /note="sequence: GCTACCTCCGCCACCg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1160..1174
                     /label="GGGGS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1160..1174
                     /label="oCK088"
                     /note="sequence: GGTGGCGGAGGTAGC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(1160..1174)
                     /label="oCD444"
                     /note="sequence: GCTACCTCCGCCACC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          1160..1194
                     /label="oCK209"
                     /note="sequence: GGTGGCGGAGGTAGCCAAAAAGTTGCGCAGCTGAA"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1175..1336
                     /label="Synzip 6"
                     /ApEinfo_revcolor="#8329ff"
                     /ApEinfo_fwdcolor="#8329ff"
     CDS             1175..1336
                     /label="Translation 1175-1336"
                     /translation="QKVAQLKNRVAYKLKENAKLENIVARLENDNANLEKDIANLEKDIANLERDVAR"
     CDS             1337..1349
                     /label="Translation 1337-1349"
                     /translation="*AAA"
     primer          complement(1337..1356)
                     /label="oCD359"
                     /note="sequence: TTTTGCGTGGCGGCCGCTTA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(1337..1356)
                     /label="oCD215"
                     /note="sequence: ttttgcgtgGCGGCCGCTTA"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1340..1341
                     /label="NotI"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1342..1356)
                     /label="oCD049"
                     /note="sequence: ttttgcgtgGCGGCC"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1350..1363
                     /label="oRC076"
                     /note="sequence: GCccgcaaaaaaccccg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1350..1367
                     /label="oRC012.v2"
                     /note="sequence: cgcaaaaaaccccgcttc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1350..1369)
                     /label="oCD276"
                     /note="sequence: ccgaagcggggttttttgcg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     terminator      1350..1383
                     /label="BBa_B1002 terminator"
                     /ApEinfo_revcolor="#af14ff"
                     /ApEinfo_fwdcolor="#af14ff"
     primer          complement(1355..1370)
                     /label="oRC111"
                     /note="sequence: GGCGGGCTTTGCTCGAGccgaagcggggtttt"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1369..1383)
                     /label="oRC037.v2"
                     /note="sequence: GACGGAGTAAGGATCCgcgaaaaaaccccgc"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1384..1398
                     /label="Overhang"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1384..1403
                     /label="oRC001"
                     /note="sequence: GGATCCTTACTCCGTCGACA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1384..1403
                     /label="Copy of oRC001"
                     /note="sequence: GGATCCTTACTCCGTCGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(1419..1438)
                     /label="oRC080"
                     /note="sequence: AATTGAGTCGAATTCCTCGA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1799..1816)
                     /label="Seq7-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1851..1870)
                     /label="Seq8-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(1959..2641)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(2739..3398)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
       61 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
      121 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
      181 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA
      241 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC
      301 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT
      361 AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTT CAAGAATTCT GGCGAATCCT
      421 CTGACCAGCC AGAAAACGAC CTTTCTGTGG TGAAACCGGA TGCTGCAATT CAGAGCGGCA
      481 GCAAGTGGGG GACAGCAGAA GACCTGACCG CCGCAGAGTG GATGTTTGAC ATGGTGAAGA
      541 CTATCGCACC ATCAGCCAGA AAACCGAATT TTGCTGGGTG GGCTAACGAT ATCCGCCTGA
      601 TGCGTGAACG TGACGGACGT AACCACCGCG ACATGTGTGT GCTGTTCCGC TGGGCATGCC
      661 AGGACAACTT CTGGTCCGGT AACGTGCTGA GCCCGGCCAA GCTTAGCccg aaaagtgcca
      721 cctgTGGCAA TTCCGACGTC tttacggcta gctcagccct aggtattatg ctagcGAATT
      781 CATTAAAGAG GAGAAAGGTA CCatggggcc cgcttctaac tttactcagt tcgttctcgt
      841 cgacaatggc ggaactggcg acgtgactgt cgccccaagc aacttcgcta acgggatcgc
      901 tgaatggatc agctctaact cgcgttcaca ggcttacaaa gtaacctgta gcgttcgtca
      961 gagctctgcg cagaatcgca aatacaccat caaagtcgag gtgcctaaag gcgcctggcg
     1021 ttcgtactta aatatggaac taaccattcc aattttcgcc acgaattccg actgcgagct
     1081 tattgttaag gcaatgcaag gtctcctaaa agatggaaac ccgattccct cagcaatcgc
     1141 agcaaactcc ggcatctacG GTGGCGGAGG TAGCCAAAAA GTTGCGCAGC TGAAAAACCG
     1201 TGTTGCGTAC AAACTGAAAG AAAACGCGAA GCTGGAGAAC ATCGTGGCGC GTCTGGAAAA
     1261 CGACAATGCG AACCTGGAGA AAGACATTGC GAATCTCGAA AAGGACATCG CAAATCTGGA
     1321 ACGTGACGTT GCGCGTTAAG CGGCCGCcac gcaaaaaacc ccgcttcggc ggggtttttt
     1381 cgcGGATCCT TACTCCGTCG ACAAGCTTGC GGCCGCACTC GAGGAATTCG ACTCAATTAG
     1441 TTCAGTCAGT TTCAGGATAT TAGTCATCTC TACATTGATT ATGAGTATTC AGAAATTCCT
     1501 TAAATATTCT GACAAATGCT CTTTCCCTAA ACTCCCCCCA TAAAAAAACC CGCCGAAGCG
     1561 GGTTTTTACG TTATTTGCGG ATTAACGATT ACTCGTTATC AGAACCGCCC AGACCTGCGT
     1621 TCAGCAGTTC TGCCAGGCTG GCAGATGCGT CTTCCGAATT GATCCGTCGA CCGATGCCCT
     1681 TGAGAGCCTT CAACCCAGTC AGCTCCTTCC GGTGGGCGCG GGGCATGACT ATCGTCGCCG
     1741 CACTTATGAC TGTCTTCTTT ATCATGCAAC TCGTAGGACA GGTGCCGGCA GCGCTCTTCC
     1801 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT
     1861 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG
     1921 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC
     1981 CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA
     2041 AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT
     2101 CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG
     2161 GCGCTTTCTC AATGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG
     2221 CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT
     2281 CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC
     2341 AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC
     2401 TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC
     2461 GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT
     2521 TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC
     2581 TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG
     2641 AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA
     2701 ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA
     2761 CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG
     2821 ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC
     2881 CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC
     2941 AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT
     3001 AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC
     3061 GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG
     3121 CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC
     3181 GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT
     3241 TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG
     3301 TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT GCCCGGCGTC AATACGGGAT
     3361 AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCAT
//