LOCUS       pRC012                  3369 bp ds-DNA     circular     06-OCT-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    547..566
                     /label="Seq6-s"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    572..597
                     /label="oBT080"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    698..704
                     /label="Ptar promoter"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          703..728
                     /label="oBT074_JxxxextF"
                     /note="sequence: TTAGCccgaaaagtgccacctgTGGC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          708..727
                     /label="oBT031_143.J3linF"
                     /note="sequence: ccgaaaagtgccacctgTGG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(708..740)
                     /label="oJF148b"
                     /note="sequence: GACGTCGGAATTGCCAcaggtggcacttttcgg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(717..740)
                     /label="OBT046_IFFLseq4"
                     /note="sequence: GCTGACGTCGGAATTGCCACAGGTGGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(718..735)
                     /label="oBT022_143.J3linR"
                     /note="sequence: CGGAATTGCCACAGGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          725..740
                     /label="oJF291"
                     /note="sequence: TGGCAATTCCGACGTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(725..740)
                     /label="Copy of oRC002"
                     /note="sequence: GACGTCGGAATTGCCA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(725..740)
                     /label="oRC002"
                     /note="sequence: GACGTCGGAATTGCCA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(725..740)
                     /label="oJF148"
                     /note="sequence: GACGTCGGAATTGCCA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          726..755
                     /label="oRC038"
                     /note="sequence: GGCAATTCCGACGTCgatttcctgCCCGGG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          756..772
                     /label="pBT2 B1002t_R"
                     /note="sequence: gcgaaaaaaccccgccg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     terminator      complement(756..789)
                     /label="BBa_B1002 terminator"
                     /ApEinfo_revcolor="#af14ff"
                     /ApEinfo_fwdcolor="#af14ff"
     primer          770..789
                     /label="oCD276"
                     /note="sequence: ccgaagcggggttttttgcg"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(772..789)
                     /label="oRC012.v2"
                     /note="sequence: cgcaaaaaaccccgcttc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(776..789)
                     /label="oRC076"
                     /note="sequence: GCccgcaaaaaaccccg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(776..789)
                     /label="oRC076"
                     /note="sequence: GCccgcaaaaaaccccg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          783..821
                     /label="oCK204"
                     /note="sequence: ttttgcgtgGGCGCGCCTTACTCGAATTTGTGAGCCGCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    792..799
                     /label="SgsI"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(803..934)
                     /label="Synzip 5"
                     /ApEinfo_revcolor="#972fff"
                     /ApEinfo_fwdcolor="#972fff"
     CDS             complement(803..934)
                     /label="Translation 934-803"
                     /translation="NTVKELKNYIQELEERNAELKNLKEHLKFAKAELEFELAAHKFE"
     primer          complement(910..949)
                     /label="oCK207"
                     /note="sequence: GGTGGCGGAGGTAGCAACACCGTTAAAGAACTGAAAAACT"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(934..949)
                     /label="oRC015"
                     /note="sequence: GGTGGCGGAGGTAGCA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          935..949
                     /label="oCD444"
                     /note="sequence: GCTACCTCCGCCACC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(935..949)
                     /label="GGGGS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(935..949)
                     /label="Translation 949-935"
                     /translation="GGGGS"
     primer          complement(935..949)
                     /label="oCK088"
                     /note="sequence: GGTGGCGGAGGTAGC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          935..962
                     /label="oRC049"
                     /note="sequence: GCTACCTCCGCCACCCAGGCGGTGGCGA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          935..967
                     /label="oCD429"
                     /note="sequence: GCTACCTCCGCCACCCAGGCGGTGGCGATAATC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          950..964
                     /label="oRC043"
                     /note="sequence: CAGGCGGTGGCGAta"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(950..970)
                     /label="oCK694"
                     /note="sequence: GCGGATTATCGCCACCGCCTG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(950..1270)
                     /label="SoxS_dmut"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(950..1270)
                     /label="Translation 1270-950"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRTVTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFARQFDRTPADYRHRL"
     misc_feature    complement(968..970)
                     /label="S101A"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    complement(992..994)
                     /label="R93A"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(1243..1290)
                     /label="oCK386"
                     /note="sequence: TTAAAGAGGAGAAAGGTACCATGTCCCATCAGAAAATTATTCAGGATC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1268..1303)
                     /label="oRC110"
                     /note="sequence: GTGATAATGGTTGCAGCTAGCGAATTCATTAAAGAGGAGAAAGGTACCatg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1271..1297
                     /label="oCDP004"
                     /note="sequence: GGTACCTTTCTCCTCTTTAATGAATTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1271..1297)
                     /label="Bujard RBS (strong)"
                     /ApEinfo_revcolor="#db7aff"
                     /ApEinfo_fwdcolor="#db7aff"
     primer          complement(1273..1300)
                     /label="oRC022"
                     /note="sequence: GCAGGGATTGTCACAGCGAATTCATTAAAGAGGAGAAAGGTA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     RBS             complement(1277..1288)
                     /label="RBS (strong)"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_fwdcolor="#996633"
     primer          1281..1304
                     /label="oRC184"
                     /note="sequence: TCCTCTTTAATGAATTCGCTAGCA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1298..1332
                     /label="oBT104_J23107R"
                     /note="sequence: gctagcataatacctagggctgagctagccgtaaa"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        complement(1298..1332)
                     /label="J23107 promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1314..1359)
                     /label="oCK151"
                     /note="sequence: gaagcggaatatatccctagGATGCATtttacggctagctcagccc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(1333..1369)
                     /label="oRC039"
                     /note="sequence: ACGGAGTAAGGATCCggaatatatccctagGATGCAT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer          complement(1339..1364)
                     /label="oCK1194"
                     /note="sequence: GTAAGGATCCggaatatatccctagG"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1340..1344
                     /label="AvrII"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1340..1359)
                     /label="Overhang"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1355..1374
                     /label="Copy of oRC001"
                     /note="sequence: GGATCCTTACTCCGTCGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1355..1374
                     /label="oRC001"
                     /note="sequence: GGATCCTTACTCCGTCGACA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1770..1787)
                     /label="Seq7-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1822..1841)
                     /label="Seq8-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(1930..2612)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(2710..3369)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG
       61 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT
      121 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG
      181 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA
      241 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC
      301 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT
      361 AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTT CAAGAATTCT GGCGAATCCT
      421 CTGACCAGCC AGAAAACGAC CTTTCTGTGG TGAAACCGGA TGCTGCAATT CAGAGCGGCA
      481 GCAAGTGGGG GACAGCAGAA GACCTGACCG CCGCAGAGTG GATGTTTGAC ATGGTGAAGA
      541 CTATCGCACC ATCAGCCAGA AAACCGAATT TTGCTGGGTG GGCTAACGAT ATCCGCCTGA
      601 TGCGTGAACG TGACGGACGT AACCACCGCG ACATGTGTGT GCTGTTCCGC TGGGCATGCC
      661 AGGACAACTT CTGGTCCGGT AACGTGCTGA GCCCGGCCAA GCTTAGCccg aaaagtgcca
      721 cctgTGGCAA TTCCGACGTC gatttcctgC CCGGGgcgaa aaaaccccgc cgaagcgggg
      781 ttttttgcgt gGGCGCGCCT TACTCGAATT TGTGAGCCGC CAGTTCGAAT TCCAGTTCCG
      841 CTTTTGCGAA TTTCAGGTGT TCCTTCAGGT TTTTGAGTTC AGCGTTACGC TCTTCCAGCT
      901 CCTGGATGTA GTTTTTCAGT TCTTTAACGG TGTTGCTACC TCCGCCACCC AGGCGGTGGC
      961 GATAATCCGC GGGAGTGCGA TCAAACTGCC GCGCGAAAAC GCGGGAGAAG GTCTGCTGCG
     1021 AGACATAACC CAGGTCCATT GCGATATCAA AAATCGGACG CTCGGTGGTG CGCAACTCAA
     1081 CGGCGGCCAG TAACAGGCGG CGTTGGCGAA TGTAATCGCC AAGCGTCTGA TGCGTCACCG
     1141 TGCGGAACAT TCGTTGCAAG TACCACTTTG AATAGCCTGA TTTTTTTGCG ACTACATCAA
     1201 TGTTAAGCGG CTGGTCAATA TGCTCGTCAA TCCATGCGAT AAGATCCTGA ATAATTTTCT
     1261 GATGGGACAT GGTACCTTTC TCCTCTTTAA TGAATTCgct agcataatac ctagggctga
     1321 gctagccgta aaATGCATCc tagggatata ttccGGATCC TTACTCCGTC GACAAGCTTG
     1381 CGGCCGCACT CGAGGAATTC GACTCAATTA GTTCAGTCAG TTTCAGGATA TTAGTCATCT
     1441 CTACATTGAT TATGAGTATT CAGAAATTCC TTAAATATTC TGACAAATGC TCTTTCCCTA
     1501 AACTCCCCCC ATAAAAAAAC CCGCCGAAGC GGGTTTTTAC GTTATTTGCG GATTAACGAT
     1561 TACTCGTTAT CAGAACCGCC CAGACCTGCG TTCAGCAGTT CTGCCAGGCT GGCAGATGCG
     1621 TCTTCCGAAT TGATCCGTCG ACCGATGCCC TTGAGAGCCT TCAACCCAGT CAGCTCCTTC
     1681 CGGTGGGCGC GGGGCATGAC TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA
     1741 CTCGTAGGAC AGGTGCCGGC AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC
     1801 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
     1861 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
     1921 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
     1981 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
     2041 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
     2101 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA
     2161 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
     2221 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
     2281 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
     2341 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG
     2401 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
     2461 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
     2521 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
     2581 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
     2641 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
     2701 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
     2761 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
     2821 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC
     2881 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
     2941 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
     3001 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
     3061 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
     3121 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
     3181 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
     3241 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
     3301 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
     3361 AGTGCTCAT
//