LOCUS       pRC027                  3785 bp ds-DNA     circular     26-APR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    533..552
                     /label="Seq6-s"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    558..583
                     /label="oBT080"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          589..608
                     /label="oDA081"
                     /note="sequence: CGTGAACGTGACGGACGTAA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    684..690
                     /label="Ptar promoter"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          689..714
                     /label="oBT074_JxxxextF"
                     /note="sequence: TTAGCccgaaaagtgccacctgTGGC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          694..713
                     /label="oBT031_143.J3linF"
                     /note="sequence: ccgaaaagtgccacctgTGG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(694..726)
                     /label="oJF148b"
                     /note="sequence: GACGTCGGAATTGCCAcaggtggcacttttcgg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(703..726)
                     /label="OBT046_IFFLseq4"
                     /note="sequence: GCTGACGTCGGAATTGCCACAGGTGGC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(704..721)
                     /label="oBT022_143.J3linR"
                     /note="sequence: CGGAATTGCCACAGGTGG"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          711..726
                     /label="oJF291"
                     /note="sequence: TGGCAATTCCGACGTC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(711..726)
                     /label="Copy of oRC002"
                     /note="sequence: GACGTCGGAATTGCCA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(711..726)
                     /label="oRC002"
                     /note="sequence: GACGTCGGAATTGCCA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(711..726)
                     /label="oJF148"
                     /note="sequence: GACGTCGGAATTGCCA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          712..741
                     /label="oRC038"
                     /note="sequence: GGCAATTCCGACGTCgatttcctgCCCGGG"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          742..758
                     /label="pBT2 B1002t_R"
                     /note="sequence: gcgaaaaaaccccgccg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     terminator      complement(742..775)
                     /label="BBa_B1002 terminator"
                     /ApEinfo_revcolor="#af14ff"
                     /ApEinfo_fwdcolor="#af14ff"
     primer          756..775
                     /label="oCD276"
                     /note="sequence: ccgaagcggggttttttgcg"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(758..775)
                     /label="oRC012.v2"
                     /note="sequence: cgcaaaaaaccccgcttc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(762..775)
                     /label="oRC076"
                     /note="sequence: GCccgcaaaaaaccccg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(762..775)
                     /label="oRC076"
                     /note="sequence: GCccgcaaaaaaccccg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             complement(776..787)
                     /label="Translation 787-776"
                     /translation="*AAA"
     misc_feature    complement(788..1336)
                     /label="PYL1"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(788..1336)
                     /label="Translation 1336-788"
                     /translation="MGGGAPTQDEFTQLSQSIAEFHTYQLGNGRCSSLLAQRIHAPPETVWSVVRRFDRPQIYKHFIKSCNVSEDFEMRVGCTRDVNVISGLPANTSRERLDLLDDDRRVTGFSITGGEHRLRNYKSVTTVHRFEKEEEEERIWTVVLESYVVDVPEGNSEEDTRLFADTVIRLNLQKLASITEAMN"
     primer          complement(1336..1351)
                     /label="oRC015"
                     /note="sequence: GGTGGCGGAGGTAGCA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1337..1351
                     /label="oCD444"
                     /note="sequence: GCTACCTCCGCCACC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(1337..1351)
                     /label="GGGGS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             complement(1337..1351)
                     /label="Translation 1351-1337"
                     /translation="GGGGS"
     primer          complement(1337..1351)
                     /label="oCK088"
                     /note="sequence: GGTGGCGGAGGTAGC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1337..1364
                     /label="oRC049"
                     /note="sequence: GCTACCTCCGCCACCCAGGCGGTGGCGA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1337..1369
                     /label="oCD429"
                     /note="sequence: GCTACCTCCGCCACCCAGGCGGTGGCGATAATC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1352..1366
                     /label="oRC043"
                     /note="sequence: CAGGCGGTGGCGAta"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    complement(1352..1672)
                     /label="SoxS_dmut"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     CDS             complement(1352..1672)
                     /label="Translation 1672-1352"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRTVTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFARQFDRTPADYRHRL"
     misc_feature    complement(1370..1372)
                     /label="S101A"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     misc_feature    complement(1394..1396)
                     /label="R93A"
                     /ApEinfo_revcolor="#ff0080"
                     /ApEinfo_fwdcolor="#ff0080"
     primer          complement(1645..1692)
                     /label="oCK386"
                     /note="sequence: TTAAAGAGGAGAAAGGTACCATGTCCCATCAGAAAATTATTCAGGATC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          1673..1699
                     /label="oCDP004"
                     /note="sequence: GGTACCTTTCTCCTCTTTAATGAATTC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1673..1699)
                     /label="Bujard RBS (strong)"
                     /ApEinfo_revcolor="#db7aff"
                     /ApEinfo_fwdcolor="#db7aff"
     RBS             complement(1679..1690)
                     /label="RBS (strong)"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_fwdcolor="#996633"
     promoter        complement(1700..1734)
                     /label="J23107 promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1716..1761)
                     /label="oCK151"
                     /note="sequence: gaagcggaatatatccctagGATGCATtttacggctagctcagccc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(1735..1771)
                     /label="oRC039"
                     /note="sequence: ACGGAGTAAGGATCCggaatatatccctagGATGCAT"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1742..1746
                     /label="AvrII"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1742..1761)
                     /label="Overhang"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1757..1776
                     /label="oRC001"
                     /note="sequence: GGATCCTTACTCCGTCGACA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2172..2189)
                     /label="Seq7-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(2224..2243)
                     /label="Seq8-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(2332..3014)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(3112..3771)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA
       61 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA
      121 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC
      181 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG
      241 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC
      301 GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT GACATTAACC TATAAAAATA
      361 GGCGTATCAC GAGGCCCTTT CGTCTTCAAG AATTCTGGCG AATCCTCTGA CCAGCCAGAA
      421 AACGACCTTT CTGTGGTGAA ACCGGATGCT GCAATTCAGA GCGGCAGCAA GTGGGGGACA
      481 GCAGAAGACC TGACCGCCGC AGAGTGGATG TTTGACATGG TGAAGACTAT CGCACCATCA
      541 GCCAGAAAAC CGAATTTTGC TGGGTGGGCT AACGATATCC GCCTGATGCG TGAACGTGAC
      601 GGACGTAACC ACCGCGACAT GTGTGTGCTG TTCCGCTGGG CATGCCAGGA CAACTTCTGG
      661 TCCGGTAACG TGCTGAGCCC GGCCAAGCTT AGCccgaaaa gtgccacctg TGGCAATTCC
      721 GACGTCgatt tcctgCCCGG Ggcgaaaaaa ccccgccgaa gcggggtttt ttgcgGGCGG
      781 CCGCTTAgtt catagcttca gtgatcgaag caagtttctg aagattcaat ctaataaccg
      841 tatcagcaaa caatctcgta tcttcctccg aattaccttc cggtacatca acaacataag
      901 attccaaaac aacggtccag atcctttctt cttcttcttc tttctcaaat ctatgaaccg
      961 tcgtaaccga tttataattc ctcagcctat gttcaccacc ggttatacta aacccagtca
     1021 ctctccgatc atcgtccaac agatctaatc tctctcgaga cgtattcgcc ggtaatccac
     1081 ttatcacgtt cacgtcgcgc gtgcatccca ctcgcatctc gaaatcttca ctcacgttac
     1141 agcttttgat gaagtgtttg taaatctgtg gcctatcgaa acgtctcacc acggaccata
     1201 ctgtttccgg cggcgcgtgg attcgctgag ctaggagaga tgagcaacgg ccgttaccga
     1261 gttggtacgt gtggaactcg gcgattgatt gggagagttg ggtgaattcg tcttgagttg
     1321 gcgcgccccc acccatGCTA CCTCCGCCAC CCAGGCGGTG GCGATAATCC GCGGGAGTGC
     1381 GATCAAACTG CCGCGCGAAA ACGCGGGAGA AGGTCTGCTG CGAGACATAA CCCAGGTCCA
     1441 TTGCGATATC AAAAATCGGA CGCTCGGTGG TGCGCAACTC AACGGCGGCC AGTAACAGGC
     1501 GGCGTTGGCG AATGTAATCG CCAAGCGTCT GATGCGTCAC CGTGCGGAAC ATTCGTTGCA
     1561 AGTACCACTT TGAATAGCCT GATTTTTTTG CGACTACATC AATGTTAAGC GGCTGGTCAA
     1621 TATGCTCGTC AATCCATGCG ATAAGATCCT GAATAATTTT CTGATGGGAC ATGGTACCTT
     1681 TCTCCTCTTT AATGAATTCg ctagcataat acctagggct gagctagccg taaaATGCAT
     1741 Cctagggata tattccGGAT CCTTACTCCG TCGACAAGCT TGCGGCCGCA CTCGAGGAAT
     1801 TCGACTCAAT TAGTTCAGTC AGTTTCAGGA TATTAGTCAT CTCTACATTG ATTATGAGTA
     1861 TTCAGAAATT CCTTAAATAT TCTGACAAAT GCTCTTTCCC TAAACTCCCC CCATAAAAAA
     1921 ACCCGCCGAA GCGGGTTTTT ACGTTATTTG CGGATTAACG ATTACTCGTT ATCAGAACCG
     1981 CCCAGACCTG CGTTCAGCAG TTCTGCCAGG CTGGCAGATG CGTCTTCCGA ATTGATCCGT
     2041 CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG
     2101 ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG
     2161 GCAGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
     2221 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC
     2281 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT
     2341 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     2401 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     2461 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     2521 TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     2581 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     2641 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     2701 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     2761 GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA
     2821 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     2881 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     2941 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG
     3001 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG
     3061 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT
     3121 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT
     3181 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT
     3241 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG
     3301 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG
     3361 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT
     3421 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC
     3481 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT
     3541 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC
     3601 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA
     3661 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC
     3721 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA
     3781 ACGTT
//