LOCUS       pRC042                  3502 bp ds-DNA     circular     19-APR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      complement(2..684)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(18..37)
                     /label="oCK216"
                     /note="sequence: gggcggagcctatggaaaaa"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             complement(782..1441)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1739..1770
                     /label="oRC031"
                     /note="sequence: tccgcgcacatttccccgaaaagtgccacctg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1767..1794)
                     /label="oCK321"
                     /note="sequence: tgataataatggtttcttagacgtcagg"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    1988..2007
                     /label="Seq6-s"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2044..2063
                     /label="oDA081"
                     /note="sequence: CGTGAACGTGACGGACGTAA"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    2139..2145
                     /label="Ptar promoter"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     primer          2160..2178
                     /label="oDA184"
                     /note="sequence: cacctgTGGCAATTCCGAC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          2167..2199
                     /label="oRC013"
                     /note="sequence: GGCAATTCCGACGTCtttacggctagctcagcc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          2167..2203
                     /label="oRC144"
                     /note="sequence: atcCCCACCCCTGCAtttacggctagctcagtcctag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          2182..2200
                     /label="oRC120"
                     /note="sequence: ggtggtgaatcCCCACCCCTGCAGtttacggctagctcagccc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2182..2216
                     /label="J23107"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    2217..2243
                     /label="Bujard RBS (strong)"
                     /ApEinfo_revcolor="#db7aff"
                     /ApEinfo_fwdcolor="#db7aff"
     RBS             2226..2237
                     /label="RBS (strong)"
                     /ApEinfo_revcolor="#996633"
                     /ApEinfo_fwdcolor="#996633"
     primer          complement(2246..2262)
                     /label="oRC140"
                     /note="sequence: agttagaagcgggcccc"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    2253..2599
                     /label="MCPdelFG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             2253..2599
                     /label="Translation 2253-2599"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLNMELTIPIFATNSDCELIVKAMQGLLKDGNPIPSAIAANSGI"
     CDS             2598..2600
                     /label="Translation 2598-2600"
                     /translation="Y"
     primer          complement(2600..2615)
                     /label="oRC009"
                     /note="sequence: GCTACCTCCGCCACCg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2601..2615
                     /label="overhang"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2601..2615
                     /label="fRC008"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             2601..2615
                     /label="Translation 2601-2615"
                     /translation="GGGGS"
     misc_feature    2616..2891
                     /label="GAI"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     CDS             2616..2891
                     /label="Translation 2616-2891"
                     /translation="MKRDHHHHHHQDKKTMMMNEEDDGNGMDELLAVLGYKVRSSEMADVAQKLEQLEVMMSNVQEDDLSQLATETVHYNPAELYTWLDSMLTDLN"
     CDS             2892..2894
                     /label="Translation 2892-2894"
                     /translation="*"
     terminator      2895..2908
                     /label="BBa_B1002 terminator"
                     /ApEinfo_revcolor="#af14ff"
                     /ApEinfo_fwdcolor="#af14ff"
     misc_feature    2895..2908
                     /label="fRC008"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2895..2908
                     /label="oRC012"
                     /note="sequence: ccgcaaaaaaccccg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    complement(2895..2908)
                     /label="overhang"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2895..2912
                     /label="oRC012.v2"
                     /note="sequence: cgcaaaaaaccccgcttc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(2895..2914)
                     /label="oCD276"
                     /note="sequence: ccgaagcggggttttttgcg"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     terminator      2909..2928
                     /label="BBa_B1002 terminator"
                     /ApEinfo_revcolor="#af14ff"
                     /ApEinfo_fwdcolor="#af14ff"
     primer          complement(2914..2944)
                     /label="oRC037.v2"
                     /note="sequence: GACGGAGTAAGGATCCgcgaaaaaaccccgc"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          complement(2915..2943)
                     /label="Copy of oRC004"
                     /note="sequence: ACGGAGTAAGGATCCgcgaaaaaaccccg"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          2929..2948
                     /label="Copy of oRC001"
                     /note="sequence: GGATCCTTACTCCGTCGACA"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(2964..2983)
                     /label="oRC080"
                     /note="sequence: AATTGAGTCGAATTCCTCGA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3179..3196)
                     /label="oDA178"
                     /note="sequence: GACGCATCTGCCAGCCTG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          3196..3213
                     /label="oRC097"
                     /note="sequence: CTTCCGAATTGATCCGTC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          3197..3216
                     /label="oDA180"
                     /note="sequence: TTCCGAATTGATCCGTCGAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(3344..3361)
                     /label="Seq7-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(3396..3415)
                     /label="Seq8-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC
       61 GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC
      121 CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG
      181 CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCAATGCTC ACGCTGTAGG TATCTCAGTT
      241 CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC
      301 GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC
      361 CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG
      421 AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG
      481 CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA
      541 CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG
      601 GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT
      661 CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA
      721 ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT
      781 ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG
      841 TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA
      901 GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC
      961 AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT
     1021 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
     1081 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
     1141 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
     1201 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
     1261 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
     1321 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
     1381 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
     1441 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
     1501 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
     1561 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
     1621 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
     1681 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
     1741 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT
     1801 TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT TCAAGAATTC TGGCGAATCC
     1861 TCTGACCAGC CAGAAAACGA CCTTTCTGTG GTGAAACCGG ATGCTGCAAT TCAGAGCGGC
     1921 AGCAAGTGGG GGACAGCAGA AGACCTGACC GCCGCAGAGT GGATGTTTGA CATGGTGAAG
     1981 ACTATCGCAC CATCAGCCAG AAAACCGAAT TTTGCTGGGT GGGCTAACGA TATCCGCCTG
     2041 ATGCGTGAAC GTGACGGACG TAACCACCGC GACATGTGTG TGCTGTTCCG CTGGGCATGC
     2101 CAGGACAACT TCTGGTCCGG TAACGTGCTG AGCCCGGCCA AGCTTAGCcc gaaaagtgcc
     2161 acctgTGGCA ATTCCGACGT Ctttacggct agctcagccc taggtattat gctagcGAAT
     2221 TCATTAAAGA GGAGAAAGGT ACCatggggc ccgcttctaa ctttactcag ttcgttctcg
     2281 tcgacaatgg cggaactggc gacgtgactg tcgccccaag caacttcgct aacgggatcg
     2341 ctgaatggat cagctctaac tcgcgttcac aggcttacaa agtaacctgt agcgttcgtc
     2401 agagctctgc gcagaatcgc aaatacacca tcaaagtcga ggtgcctaaa ggcgcctggc
     2461 gttcgtactt aaatatggaa ctaaccattc caattttcgc cacgaattcc gactgcgagc
     2521 ttattgttaa ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc tcagcaatcg
     2581 cagcaaactc cggcatctac GGTGGCGGAG GTAGCATGAA GCGCGATCAT CATCACCACC
     2641 ACCACCAGGA TAAAAAGACG ATGATGATGA ATGAGGAAGA TGATGGAAAC GGGATGGACG
     2701 AATTGCTGGC AGTGCTGGGA TATAAGGTGC GTTCGTCCGA AATGGCAGAT GTTGCTCAGA
     2761 AATTGGAGCA GTTAGAAGTA ATGATGAGTA ACGTTCAAGA AGATGATCTT TCACAGTTAG
     2821 CGACCGAAAC TGTCCACTAC AACCCTGCTG AGCTTTACAC TTGGTTGGAC TCCATGCTTA
     2881 CCGATCTTAA Ctgacgcaaa aaaccccgct tcggcggggt tttttcgcGG ATCCTTACTC
     2941 CGTCGACAAG CTTGCGGCCG CACTCGAGGA ATTCGACTCA ATTAGTTCAG TCAGTTTCAG
     3001 GATATTAGTC ATCTCTACAT TGATTATGAG TATTCAGAAA TTCCTTAAAT ATTCTGACAA
     3061 ATGCTCTTTC CCTAAACTCC CCCCATAAAA AAACCCGCCG AAGCGGGTTT TTACGTTATT
     3121 TGCGGATTAA CGATTACTCG TTATCAGAAC CGCCCAGACC TGCGTTCAGC AGTTCTGCCA
     3181 GGCTGGCAGA TGCGTCTTCC GAATTGATCC GTCGACCGAT GCCCTTGAGA GCCTTCAACC
     3241 CAGTCAGCTC CTTCCGGTGG GCGCGGGGCA TGACTATCGT CGCCGCACTT ATGACTGTCT
     3301 TCTTTATCAT GCAACTCGTA GGACAGGTGC CGGCAGCGCT CTTCCGCTTC CTCGCTCACT
     3361 GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA
     3421 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG
     3481 CAAAAGGCCA GGAACCGTAA AA
//