LOCUS       pDA010.EL222            3287 bp ds-DNA     circular     07-NOV-2022
DEFINITION  .
COMMENT     mported using the Genbank importer. File name: P28a-MscL-eGFP.str
            From plasmid pBEST-Ptar-UTR1-MotA-eGFP, insert MscL gene (no stop)
            between Acc65I-ApaI. ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer          574..591
                     /label="T7promoter_18-23"
                     /note="sequence: CCAGCGTTTCTGGGTGAG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          574..591
                     /label="T7promoter_18-23"
                     /note="sequence: CCAGCGTTTCTGGGTGAG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          629..649
                     /label="oBT15 pAmp seqF"
                     /note="sequence: aagggcgacacggaaatgttg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          660..715
                     /label="oBT045_IFFL_seq3"
                     /note="sequence: ctcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcgg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          662..696
                     /label="pBT3 Jx ext_F"
                     /note="sequence: cttcctttttcaatattattgaagcatttatcagg"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          706..727
                     /label="arsB fw"
                     /note="sequence: TCATGAGCGGATACATATTTGA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(714..772)
                     /label="oBT041_143linR_long"
                     /note="sequence: ggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatcc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          727..761
                     /label="oBT247_pBT001_linF"
                     /note="sequence: aatgtatttagaaaaataaacaaataggggttccg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          734..759
                     /label="rbsR homology fw"
                     /note="sequence: TTAGAAAAATAAACAAATAGGGGTTC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          734..759
                     /label="rbsR homology fw"
                     /note="sequence: TTAGAAAAATAAACAAATAGGGGTTC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          complement(754..772)
                     /label="oBT032_pJF143.J3linR"
                     /note="sequence: ggaaatgtgcgcggaaccc"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    790..824
                     /label="J23106"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          790..824
                     /label="oBT118_J23106F"
                     /note="sequence: tttacggctagctcagtcctaggtatagtgctagc"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(790..824)
                     /label="oBT119_J23106R"
                     /note="sequence: gctagcactatacctaggactgagctagccgtaaa"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          808..867
                     /label="oBT183_EL222ampF"
                     /note="sequence: CTAGGTATAGTGCTAGCCTAGAGAAAGAGGAGAAATACTAGATGTTGGATATGGGACAAG"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    824..824
                     /label="J23106"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    831..842
                     /label="BBa_B0034 (rbs34)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    843..1679
                     /label="ordered sequence"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    849..1526
                     /label="EL222"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             849..1526
                     /label="Translation 849-1526"
                     /translation="MLDMGQDRPIDGSGAPGADDTRVEVQPPAQWVLDLIEASPIASVVSDPRLADNPLIAINQAFTDLTGYSEEECVGRNCRFLAGSGTEPWLTDKIRQGVREHKPVLVEILNYKKDGTPFRNAVLVAPIYDDDDELLYFLGSQVEVDDDQPNMGMARRERAAEMLKTLSPRQLEVTTLVASGLRNKEVAARLGLSEKTVKMHRGLVMEKLNLKTSADLVRIAVEAGI*"
     misc_feature    897..916
                     /label="sgRNA?"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    930..949
                     /label="sgRNA?"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(1161..1184)
                     /label="oBT252_EL222seq_R"
                     /note="sequence: CTTGTAGTTCAGGATCTCGACCAG"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1527..1547
                     /label="oJF466"
                     /note="sequence: ggatccaaactcgagtaagga"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1527..1547)
                     /label="oJF241"
                     /note="sequence: tccttactcgagtttggatcc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(1527..1551)
                     /label="oCD046"
                     /note="sequence: gagatccttactcgagtttggatcc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1544..1679
                     /label="BBa_B0015_dblT"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1545..1565)
                     /label="oPC003"
                     /note="sequence: TTATTTGATGCCTGGAGATCC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1551..1574
                     /label="pIDF56_down_FP"
                     /note="sequence: CCAGGCATCAAATAAAACGAAAGG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1551..1575
                     /label="oCD018"
                     /note="sequence: CCAGGCATCAAATAAAACGAAAGGC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1551..1575
                     /label="oCD018"
                     /note="sequence: CCAGGCATCAAATAAAACGAAAGGC"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     primer          1581..1598
                     /label="rbsAR 5' cPCR rev"
                     /note="sequence: cgaaagactgggcctttc"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1657..1679)
                     /label="pIDF30_pol1-pol2_RP (defunct)"
                     /note="sequence: tataaacgcagaaaggcccaccc"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          1669..1695
                     /label="oBT156_009linF_RFPint"
                     /note="sequence: GTGCTTTTTTTaacgcatgagAAAGCC"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer          1680..1698
                     /label="pIDF38.3lacUV5lacI_prom_RP"
                     /note="sequence: aacgcatgagAAAGCCCCC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          1680..1706
                     /label="oBT152_009linFt"
                     /note="sequence: aacgcatgagAAAGCCCCCGGAAGATC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     terminator      1680..1732
                     /label="ECK120033736 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(1716..1757)
                     /label="oBT077_gscRNAextR"
                     /note="sequence: AAGCTTGTCGACGGAGTAAGGATCCgcgcaataaaAAAGCCC"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1740..1781
                     /label="oBT078_pBestlinF"
                     /note="sequence: TACTCCGTCGACAAGCTTGCGGCCGCACTCGAGGAATTCGAC"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(2148..2165)
                     /label="Seq7-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(2200..2219)
                     /label="Seq8-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          2200..2225
                     /label="pIDFxx_RFP_RP"
                     /note="sequence: ggtatcagctcactcaaaggcggtaa"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2200..2225)
                     /label="pIDFxx_RFP_FP"
                     /note="sequence: ttaccgcctttgagtgagctgatacc"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(2207..2226)
                     /label="VR"
                     /note="sequence: ATTACCGCCTTTGAGTGAGC"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          2263..2309
                     /label="pIDF33a_down_FP"
                     /note="sequence: GAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(2265..2285)
                     /label="pIDF33_down_RP"
                     /note="sequence: GCTGGCCTTTTGCTCACATGT"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      complement(2308..2990)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(2358..2380)
                     /label="38_lux_seq"
                     /note="sequence: ctgacttgagcgtcgatttttgt"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(2456..2473)
                     /label="ColE1_rev_seq"
                     /note="sequence: ggtaagcggcagggtcgg"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2982..3005)
                     /label="oJF134"
                     /note="sequence: Tcctttttgataatctcatgacca"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          2991..3016
                     /label="oJF131"
                     /note="sequence: GATTATCAAAAAGGATCTTCACCTAG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          3077..3100
                     /label="oJF178"
                     /note="sequence: Tctgacagttaccaatgcttaatc"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             complement(3088..460)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             complement(3088..460)
                     /label="Translation 460-3088"
                     /translation="MSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW"
     primer          complement(3138..3159)
                     /label="oCD034"
                     /note="sequence: GGGAGTCAGGCAACTATGGATG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC TATTAATTGT TGCCGGGAAG
       61 CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT TGTTGCCATT GCTACAGGCA
      121 TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG CTCCGGTTCC CAACGATCAA
      181 GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT TAGCTCCTTC GGTCCTCCGA
      241 TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT GGTTATGGCA GCACTGCATA
      301 ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT GACTGGTGAG TACTCAACCA
      361 AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC TTGCCCGGCG TCAATACGGG
      421 ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT CATTGGAAAA CGTTCTTCGG
      481 GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG TTCGATGTAA CCCACTCGTG
      541 CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT TTCTGGGTGA GCAAAAACAG
      601 GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG GAAATGTTGA ATACTCATAC
      661 TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
      721 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCccgaaaag
      781 tgccacctgT TTACGGCTAG CTCAGTCCTA GGTATAGTGC TAGCCTAGAG AAAGAGGAGA
      841 AATACTAGAT GTTGGATATG GGACAAGATC GGCCGATCGA TGGAAGTGGG GCACCCGGGG
      901 CAGACGACAC ACGCGTTGAG GTGCAACCGC CGGCGCAGTG GGTCCTCGAC CTGATCGAGG
      961 CCAGCCCGAT CGCATCGGTC GTGTCCGATC CGCGTCTCGC CGACAATCCG CTGATCGCCA
     1021 TCAACCAGGC CTTCACCGAC CTGACCGGCT ATTCCGAAGA AGAATGCGTC GGCCGCAATT
     1081 GCCGATTCCT GGCAGGTTCC GGCACCGAGC CGTGGCTGAC CGACAAGATC CGCCAAGGCG
     1141 TGCGCGAGCA CAAGCCGGTG CTGGTCGAGA TCCTGAACTA CAAGAAGGAC GGCACGCCGT
     1201 TCCGCAATGC CGTGCTCGTT GCACCGATCT ACGATGACGA CGACGAGCTT CTCTATTTCC
     1261 TCGGCAGCCA GGTCGAAGTC GACGACGACC AGCCCAACAT GGGCATGGCG CGCCGCGAAC
     1321 GCGCCGCGGA AATGCTCAAG ACGCTGTCGC CGCGCCAGCT CGAGGTTACG ACGCTGGTGG
     1381 CATCGGGCTT GCGCAACAAG GAAGTGGCGG CCCGGCTCGG CCTGTCGGAG AAAACCGTCA
     1441 AGATGCACCG CGGGCTGGTG ATGGAAAAGC TCAACCTGAA GACCAGCGCC GATCTGGTGC
     1501 GCATTGCCGT CGAAGCCGGA ATCTAAGGAT CCAAACTCGA GTAAGGATCT CCAGGCATCA
     1561 AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT GTTTGTCGGT
     1621 GAACGCTCTC TACTAGAGTC ACACTGGCTC ACCTTCGGGT GGGCCTTTCT GCGTTTATAa
     1681 acgcatgagA AAGCCCCCGG AAGATCACCT TCCGGGGGCT TTtttattgc gcGGATCCTT
     1741 ACTCCGTCGA CAAGCTTGCG GCCGCACTCG AGGAATTCGA CTCAATTAGT TCAGTCAGTT
     1801 TCAGGATATT AGTCATCTCT ACATTGATTA TGAGTATTCA GAAATTCCTT AAATATTCTG
     1861 ACAAATGCTC TTTCCCTAAA CTCCCCCCAT AAAAAAACCC GCCGAAGCGG GTTTTTACGT
     1921 TATTTGCGGA TTAACGATTA CTCGTTATCA GAACCGCCCA GACCTGCGTT CAGCAGTTCT
     1981 GCCAGGCTGG CAGATGCGTC TTCCGAATTG ATCCGTCGAC CGATGCCCTT GAGAGCCTTC
     2041 AACCCAGTCA GCTCCTTCCG GTGGGCGCGG GGCATGACTA TCGTCGCCGC ACTTATGACT
     2101 GTCTTCTTTA TCATGCAACT CGTAGGACAG GTGCCGGCAG CGCTCTTCCG CTTCCTCGCT
     2161 CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC
     2221 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG
     2281 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG
     2341 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG
     2401 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC
     2461 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA
     2521 ATGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT
     2581 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC
     2641 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG
     2701 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC
     2761 TAGAAGGACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT
     2821 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA
     2881 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG
     2941 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA
     3001 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT
     3061 ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC
     3121 GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT
     3181 ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC
     3241 GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGC
//