LOCUS       pBT009.J1.119.DA9       3124 bp ds-DNA     circular     31-MAR-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    968..987
                     /label="Seq6-s"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1119..1125
                     /label="Ptar promoter"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     misc_feature    1162..1331
                     /label="J1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    1332..1337
                     /label=-35
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    1332..1366
                     /label="BBa_J23119(SpeI)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1355..1360
                     /label=-10
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1367..1386
                     /label="DA9 spacer"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          1387..1427
                     /label="oDA094.scRNA-F"
                     /note="sequence: GTTTTAGAGCTAGAAATAGCAAGTTAAAATAAGGCTAGTCC"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1387..1428
                     /label="JAD designed 3' chimera "
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(1387..1446)
                     /label="oJF486"
                     /note="sequence: ACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer          complement(1406..1430)
                     /label="oJF136"
                     /note="sequence: AACGGACTAGCCTTATTTTAACTTG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1416..1439
                     /label="oJF135"
                     /note="sequence: TAAGGCTAGTCCGTTATCAACTTG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1417..1433)
                     /label="oDA123"
                     /note="sequence: TTCCATATAAACTCCTTATGTTACTTTTTCAAGTTGGATAACGGACTAGCCTT"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1420..1446)
                     /label="oJF125"
                     /note="sequence: ACTTTTTCAAGTTGATAACGGACTAGC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1420..1446)
                     /label="oJF127"
                     /note="sequence: ACTTTTTCAAGTTGATAACGGACTAGC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1424..1447)
                     /label="oJF129"
                     /note="sequence: CACTTTTTCAAGTTGATAACGGAC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1433..1465
                     /label="oJF130"
                     /note="sequence: CAACTTGAAAAAGTGGCACATGAGGATCACCCA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1434..1447
                     /label="Hairpin3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1448..1470
                     /label="MS2 +2Bp extension"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1471..1477
                     /label="SUP4"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     terminator      1478..1530
                     /label="ECK120033736 terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(1506..1530)
                     /label="oCK434"
                     /note="sequence: gtctGGTCTCgtgctAGTTGCATCGTACGTCGGTGgcgcaataaaAAAGCCCCCGGAAGG"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(1510..1530)
                     /label="oCK246"
                     /note="sequence: agcaaaacccgtaccctaggcgcaataaaAAAGCCCCCGG"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1946..1963)
                     /label="Seq7-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(1998..2017)
                     /label="Seq8-as"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      complement(2106..2788)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             complement(2886..421)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG
       61 TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA
      121 GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG
      181 TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA
      241 TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG
      301 TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT
      361 CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA
      421 TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA
      481 GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG
      541 TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC
      601 GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT
      661 ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC
      721 CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT
      781 TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT TCAAGAATTC TGGCGAATCC
      841 TCTGACCAGC CAGAAAACGA CCTTTCTGTG GTGAAACCGG ATGCTGCAAT TCAGAGCGGC
      901 AGCAAGTGGG GGACAGCAGA AGACCTGACC GCCGCAGAGT GGATGTTTGA CATGGTGAAG
      961 ACTATCGCAC CATCAGCCAG AAAACCGAAT TTTGCTGGGT GGGCTAACGA TATCCGCCTG
     1021 ATGCGTGAAC GTGACGGACG TAACCACCGC GACATGTGTG TGCTGTTCCG CTGGGCATGC
     1081 CAGGACAACT TCTGGTCCGG TAACGTGCTG AGCCCGGCCA AGCTTAGCcc gaaaagtgcc
     1141 acctgTGGCA ATTCCGACGT CGCCTACGGT ATCCACCGGA GACCTATGGC AGCCTCCGGC
     1201 CGCCATAGGA CACCTTTGGT TGCCAAGGGT GACCTATGGT GACCATGGGC CACCACGGGC
     1261 GACCTCAGGT ATCCTGCGGT GTCCTGCGGT TACCAAAGGC GTCCTTTGGG TTCCACCGGA
     1321 TACCTCCGGA CTTGACAgct agctcagtcc taggTATAAT actagtTGCA GCCACGTGTG
     1381 AGAGTTGTTT TAGAGCTAGA AATAGCAAGT TAAAATAAGG CTAGTCCGTT ATCAACTTGA
     1441 AAAAGTGGCA CATGAGGATC ACCCATGTGC TTTTTTTaac gcatgagAAA GCCCCCGGAA
     1501 GATCACCTTC CGGGGGCTTT tttattgcgc GGATCCTTAC TCCGTCGACA AGCTTGCGGC
     1561 CGCACTCGAG GAATTCGACT CAATTAGTTC AGTCAGTTTC AGGATATTAG TCATCTCTAC
     1621 ATTGATTATG AGTATTCAGA AATTCCTTAA ATATTCTGAC AAATGCTCTT TCCCTAAACT
     1681 CCCCCCATAA AAAAACCCGC CGAAGCGGGT TTTTACGTTA TTTGCGGATT AACGATTACT
     1741 CGTTATCAGA ACCGCCCAGA CCTGCGTTCA GCAGTTCTGC CAGGCTGGCA GATGCGTCTT
     1801 CCGAATTGAT CCGTCGACCG ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT
     1861 GGGCGCGGGG CATGACTATC GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG
     1921 TAGGACAGGT GCCGGCAGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
     1981 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA
     2041 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
     2101 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
     2161 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
     2221 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
     2281 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT GCTCACGCTG TAGGTATCTC
     2341 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
     2401 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
     2461 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
     2521 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC
     2581 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
     2641 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
     2701 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
     2761 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
     2821 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
     2881 AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC
     2941 ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC
     3001 CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA
     3061 AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC
     3121 CAGT
//