LOCUS       pJethlpAKate2Cm         4919 bp    DNA     circular SYN 26-JUL-2023
DEFINITION  Cloning vector pJET1.2, complete sequence.
ACCESSION   EF694056
VERSION     EF694056.1
KEYWORDS    .
SOURCE      Cloning vector pJET1.2.
  ORGANISM  Cloning vector pJET1.2
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Bagdanaviciute,J., Zakareviciene,L. and Lubys,A.
  TITLE     Improved positive selection cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Lubys,A., Bagdanaviciute,J. and Zakareviciene,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2007) Research Center, Fermentas UAB, Graiciuno
            8, Vilnius LT-02241, Lithuania
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|-14128926|                                                 
            VNTDBDATE|-14128926|                                               
            LSOWNER|                                                           
            VNTNAME|pJethlpAKate2catQ1|                                        
            VNTAUTHORNAME|User|                                                
            VNTOAUTHORNAME|UNKNOWN|                                            
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
            (SXF                                                               
             (CGexDoc "pJethlpAKate2catQ1" 0 4919                              
             
FEATURES             Location/Qualifiers
     CDS             complement(16..370)
                     /standard_name="'eco47I/T7"
     CDS             370..682
                     /standard_name="HlpAmut"
     CDS             683..1375
                     /standard_name="mKate2"
     CDS             1602..2261
                     /db_xref="SEED:fig|6666666.94651.peg.298"
                     /db_xref="GO:0008811"
                     /product="Chloramphenicol acetyltransferase"
                     /EC_number="2.3.1.28"
                     /protein_id="F4-20_00579"
                     /standard_name="catQ"
     CDS             complement(2572..2683)
                     /standard_name="eco47I/T7'"
     rep_origin      complement(3093..3707)
     gene            complement(3867..4727)
                     /gene="bla"
     CDS             complement(3867..4727)
                     /gene="bla"
                     /function="catalyzes the opening and hydrolysis of the
                     beta-lactam ring of beta-lactam antibiotics such as
                     penicillins and cephalosporins"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="ABR53873.1"
                     /standard_name="bla"
ORIGIN      
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcatgggcaa acaaacaaga tttgatcgct aaagtagcag aagctacaga
      421 attgactaag aaagactcag cagcagcagt tgaagctgta tttgcagcag tagctgacta
      481 tcttgcagct ggtgaaaaag ttcaattgat cggttttggt aactttgaag ttcgtgagcg
      541 tgcagaacgt aaaggtcgca acccacaaac tggtaaagaa atgacaattg cagcttctaa
      601 agtaccagca ttcaaagctg gtaaagctct taaagacgct gttaaaggat ccggatctgg
      661 tggagaagct gcagctaaag gatcagaact tatcaaggaa aatatgcaca tgaaattgta
      721 catggaagga acagtaaata atcaccactt taaatgtacc tcagaaggag aaggaaaacc
      781 atatgaaggt actcaaacca tgcgtattaa ggccgttgaa ggtggaccat tgccttttgc
      841 ctttgatatt cttgctacat cttttatgta cggatcaaag acttttatca atcataccca
      901 aggtatccca gatttcttta aacagtcatt tcctgaagga tttacatggg aacgtgtcac
      961 aacttatgaa gatggtggag tattgacagc aactcaagat acatctttgc aagatggttg
     1021 tcttatctac aatgtaaaga tccgtggagt taattttcca agtaatggtc ctgttatgca
     1081 gaaaaagacc cttggatggg aagcatctac cgaaacatta tatcctgccg atggtggatt
     1141 ggaaggtcgt gctgatatgg cattgaaact tgtcggtgga ggtcacctta tctgtaattt
     1201 gaagaccaca taccgttcta aaaagccagc taagaatctt aagatgcctg gtgtttacta
     1261 cgtcgatcgt cgtttagaac gtatcaaaga agcagataag gaaacttatg ttgaacagca
     1321 cgaagtagcc gtcgcacgtt attgtgattt gcctagtaaa ttgggacacc gttaataaga
     1381 attccgtatt ggtggaataa cacgaattaa taaaggatat atttgtagta gcaagtgtat
     1441 ttgttttata ttctatgacc ctatttgatt tacatgattc tatatggtat aatggacttg
     1501 cttataaata tagatgcttg gatagcagga ggttgcaaga tgatgatggc agttaaatta
     1561 gccttctaaa attaaataat aaaattttag gaggctaatt tatgaaattt aatttgatag
     1621 atatagagga ttggaataga aagccatact ttgagcatta tttaaatgcg gtaaggtgca
     1681 cttacagtat gactgcaaat ttagagataa ctagtttact gcgtgaaatt aaacttaagg
     1741 gcctgaaact gtaccctacg cttatttata tcatcacaac tgtagttaat cgtcacaagg
     1801 agttccgcac cagttttgat caaaacggta agttaggata ctgggatagt atgaacccaa
     1861 gttatactat ctttcataag gataacgaaa ctttttcaag tatttggaca gagtatgacg
     1921 agaacttccc acgtttctac tataattacc ttgaggatat cagaaactat agcgacgtta
     1981 tgaatttcat gcctaaggca ggtcaacctg ctaacacaat taatgtgtcc agcattcctt
     2041 gggtgaattt taccggattc aacttgaata tatataatga tgcaacatat ctaatcccta
     2101 tttttactat gggtaagtat tttcagcagg ataataaaat tttattacct atgtctgtac
     2161 aggtgcatca tgcggtttgc gacggttacc ataccagcag attttttaat gaggtacagg
     2221 aattagtctc aaattttgag acatggttag gagaaaaata atattaaaat gcttgtgctc
     2281 gggagttata aacaaagatc gaaaatattg ttaagatcgt gaggaagcaa agcgaccgaa
     2341 gaagtatatg ttccatctat gcaaggactt cccgagaagg cgagtgacag cgaagaccga
     2401 ttggaaggag tctgagctaa gtggagtaat atattcattc attatagaac catcatttca
     2461 aaggattaga gaaatctaat cctttttcta ggtattttta tcctaagaat ttaatatgac
     2521 aatcacgagt ttaattttta ttttggtgct atttatccta agcagagcaa gcttcaggaa
     2581 actgagacag gaattttatt aaaaatttaa attttgaaga aagttcaggg ttaatagcat
     2641 ccattttttg ctttgcaagt tcctcagcat tcttaacaaa agacgtctct tttgacatgt
     2701 ttaaagttta aacctcctgt gtgaaattgt tatccgctca caattccaca cattatacga
     2761 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     2821 gcgttgcgct cactgccaat tgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     2881 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2941 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     3001 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     3061 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     3121 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     3181 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     3241 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     3301 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     3361 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     3421 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     3481 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     3541 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     3601 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     3661 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     3721 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3781 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3841 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3901 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3961 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     4021 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     4081 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     4141 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     4201 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     4261 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     4321 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     4381 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     4441 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     4501 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     4561 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     4621 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     4681 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     4741 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4801 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     4861 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcc
//
