LOCUS       pJethlpAKate2Spc        4818 bp    DNA     circular SYN 12-NOV-2021
DEFINITION  Cloning vector pJET1.2, complete sequence.
ACCESSION   EF694056
VERSION     EF694056.1
KEYWORDS    .
SOURCE      Cloning vector pJET1.2.
  ORGANISM  Cloning vector pJET1.2
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Bagdanaviciute,J., Zakareviciene,L. and Lubys,A.
  TITLE     Improved positive selection cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Lubys,A., Bagdanaviciute,J. and Zakareviciene,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2007) Research Center, Fermentas UAB, Graiciuno
            8, Vilnius LT-02241, Lithuania
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|-14129043|                                                 
            VNTDBDATE|-14129043|                                               
            LSOWNER|                                                           
            VNTNAME|pJethlpAKate2Spc|                                          
            VNTAUTHORNAME|User|                                                
            VNTOAUTHORNAME|UNKNOWN|                                            
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
            (SXF                                                               
             (CGexDoc "pJethlpAKate2Spc" 0 4818                                
            
FEATURES             Location/Qualifiers
     CDS             complement(16..370)
                     /standard_name="'eco47I/T7"
     CDS             370..682
                     /standard_name="HlpAmut"
     CDS             683..1375
                     /standard_name="mKate2"
     CDS             1617..2381
                     /standard_name="spec"
     CDS             complement(2471..2582)
                     /standard_name="eco47I/T7'"
     rep_origin      complement(2992..3606)
     gene            complement(3766..4626)
                     /gene="bla"
     CDS             complement(3766..4626)
                     /gene="bla"
                     /function="catalyzes the opening and hydrolysis of the
                     beta-lactam ring of beta-lactam antibiotics such as
                     penicillins and cephalosporins"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="ABR53873.1"
                     /standard_name="bla"
ORIGIN      
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcatgggcaa acaaacaaga tttgatcgct aaagtagcag aagctacaga
      421 attgactaag aaagactcag cagcagcagt tgaagctgta tttgcagcag tagctgacta
      481 tcttgcagct ggtgaaaaag ttcaattgat cggttttggt aactttgaag ttcgtgagcg
      541 tgcagaacgt aaaggtcgca acccacaaac tggtaaagaa atgacaattg cagcttctaa
      601 agtaccagca ttcaaagctg gtaaagctct taaagacgct gttaaaggat ccggatctgg
      661 tggagaagct gcagctaaag gatcagaact tatcaaggaa aatatgcaca tgaaattgta
      721 catggaagga acagtaaata atcaccactt taaatgtacc tcagaaggag aaggaaaacc
      781 atatgaaggt actcaaacca tgcgtattaa ggccgttgaa ggtggaccat tgccttttgc
      841 ctttgatatt cttgctacat cttttatgta cggatcaaag acttttatca atcataccca
      901 aggtatccca gatttcttta aacagtcatt tcctgaagga tttacatggg aacgtgtcac
      961 aacttatgaa gatggtggag tattgacagc aactcaagat acatctttgc aagatggttg
     1021 tcttatctac aatgtaaaga tccgtggagt taattttcca agtaatggtc ctgttatgca
     1081 gaaaaagacc cttggatggg aagcatctac cgaaacatta tatcctgccg atggtggatt
     1141 ggaaggtcgt gctgatatgg cattgaaact tgtcggtgga ggtcacctta tctgtaattt
     1201 gaagaccaca taccgttcta aaaagccagc taagaatctt aagatgcctg gtgtttacta
     1261 cgtcgatcgt cgtttagaac gtatcaaaga agcagataag gaaacttatg ttgaacagca
     1321 cgaagtagcc gtcgcacgtt attgtgattt gcctagtaaa ttgggacacc gttaataaga
     1381 attccgaagt tatcgtaacg tgactggcaa gagatatttt taaaacaatg aataggttta
     1441 cacttacttt agttttatgg aaatgaaaga tcatatcata tataatctag aataaaatta
     1501 actaaaataa ttattatcta gataaaaaat ttagaagcca atgaaatcta taaataaact
     1561 aaattaagtt tatttaatta acaactatgg atataaaata ggtactaatc aaaatagtga
     1621 ggaggatata tttgaataca tacgaacaaa ttaataaagt gaaaaaaata cttcggaaac
     1681 atttaaaaaa taaccttatt ggtacttaca tgtttggatc aggagttgag agtggactaa
     1741 aaccaaatag tgatcttgac tttttagtcg tcgtatctga accattgaca gatcaaagta
     1801 aagaaatact tatacaaaaa attagaccta tttcaaaaaa aataggagat aaaagcaact
     1861 tacgatatat tgaattaaca attattattc agcaagaaat ggtaccgtgg aatcatcctc
     1921 ccaaacaaga atttatttat ggagaatggt tacaagagct ttatgaacaa ggatacattc
     1981 ctcagaagga attaaattca gatttaacca taatgcttta ccaagcaaaa cgaaaaaata
     2041 aaagaatata cggaaattat gacttagagg aattactacc tgatattcca ttttctgatg
     2101 tgagaagagc cattatggat tcgtcagagg aattaataga taattatcag gatgatgaaa
     2161 ccaactctat attaacttta tgccgtatga ttttaactat ggacacgggt aaaatcatac
     2221 caaaagatat tgcgggaaat gcagtggctg aatcttctcc attagaacat agggagagaa
     2281 ttttgttagc agttcgtagt tatcttggag agaatattga atggactaat gaaaatgtaa
     2341 atttaactat aaactattta aataacagat taaaaaaatt ataaaaaaat tgaaaaaatg
     2401 gtggaaacac ttttttcaat ttttttgttt tattatttaa tatttgggaa atattcattc
     2461 taattggaag cttcaggaaa ctgagacagg aattttatta aaaatttaaa ttttgaagaa
     2521 agttcagggt taatagcatc cattttttgc tttgcaagtt cctcagcatt cttaacaaaa
     2581 gacgtctctt ttgacatgtt taaagtttaa acctcctgtg tgaaattgtt atccgctcac
     2641 aattccacac attatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2701 gagctaactc acattaattg cgttgcgctc actgccaatt gctttccagt cgggaaacct
     2761 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     2821 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2881 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2941 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     3001 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     3061 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3121 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3181 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3241 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3301 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3361 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3421 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     3481 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3541 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3601 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3661 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3721 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     3781 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     3841 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     3901 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     3961 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     4021 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     4081 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     4141 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     4201 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     4261 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     4321 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     4381 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     4441 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     4501 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     4561 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     4621 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     4681 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     4741 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     4801 taggcgtatc acgaggcc
//
