LOCUS       pJetNGKmCorr            5120 bp    DNA     circular SYN 25-JUL-2023
DEFINITION  Cloning vector pJET1.2, complete sequence.
ACCESSION   EF694056
VERSION     EF694056.1
KEYWORDS    .
SOURCE      Cloning vector pJET1.2.
  ORGANISM  Cloning vector pJET1.2
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Bagdanaviciute,J., Zakareviciene,L. and Lubys,A.
  TITLE     Improved positive selection cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Lubys,A., Bagdanaviciute,J. and Zakareviciene,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2007) Research Center, Fermentas UAB, Graiciuno
            8, Vilnius LT-02241, Lithuania
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|-14064012|                                                 
            VNTDBDATE|-14065824|                                               
            LSOWNER|                                                           
            VNTNAME|pJetNGKmCorr|                                              
            VNTAUTHORNAME|User|                                                
            VNTOAUTHORNAME|UNKNOWN|                                            
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
            (SXF                                                               
             (CGexDoc "pJetNGKmCorr" 0 5120                                    
            
FEATURES             Location/Qualifiers
     CDS             complement(16..368)
                     /standard_name="eco47I/T7'"
     primer_bind     305..324
                     /standard_name="T7 promoter"
     primer_bind     305..324
                     /standard_name=738
     primer_bind     336..353
                     /standard_name=2455
     misc_feature    370..404
                     /note="Geneious type: primer"
                     /standard_name="OVL3236_hlpA_LB_F"
     CDS             373..646
                     /standard_name="hlpA"
     primer_bind     632..651
                     /standard_name=1878
     primer_bind     complement(632..651)
                     /standard_name=2552
     CDS             659..703
                     /standard_name="Translation\287-337"
     misc_feature    686..1387
                     /standard_name="mNeonGreen"
     primer_bind     798..821
                     /standard_name=2044
     primer_bind     complement(798..821)
                     /standard_name=2045
     CDS             1595..2389
                     /standard_name="kanaR"
     primer_bind     1729..1748
                     /standard_name=1939
     primer_bind     complement(1882..1901)
                     /standard_name=2425
     primer_bind     complement(1890..1907)
                     /standard_name=869
     primer_bind     2236..2254
                     /standard_name=2427
     primer_bind     complement(2372..2394)
                     /standard_name=971
     CDS             2433..2514
                     /standard_name="cm\CDS"
     primer_bind     2457..2476
                     /standard_name=291
     misc_feature    complement(2485..2514)
                     /note="Geneious type: primer"
                     /standard_name="OVL5129_Cm-int-R"
     CDS             complement(2515..2899)
                     /standard_name="eco47I/T7'"
     primer_bind     complement(2818..2844)
                     /standard_name=2042
     primer_bind     2818..2840
                     /standard_name=2516
     primer_bind     2818..2844
                     /standard_name=2499
     primer_bind     complement(2917..2940)
                     /standard_name="M13-R (-46)"
     primer_bind     complement(2965..2984)
                     /standard_name=1227
     primer_bind     complement(2983..3000)
                     /standard_name=1429
     primer_bind     complement(2985..3005)
                     /standard_name=1427
     primer_bind     complement(3180..3199)
                     /standard_name=560
     primer_bind     complement(3190..3209)
                     /standard_name="VR"
     rep_origin      complement(3294..3908)
     gene            complement(4068..4928)
                     /gene="bla"
     CDS             complement(4068..4928)
                     /gene="bla"
                     /function="catalyzes the opening and hydrolysis of the
                     beta-lactam ring of beta-lactam antibiotics such as
                     penicillins and cephalosporins"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="ABR53873.1"
                     /standard_name="bla"
     primer_bind     complement(4100..4118)
                     /standard_name=1924
     primer_bind     5016..5034
                     /standard_name=671
     primer_bind     5051..5070
                     /standard_name="VF2"
ORIGIN      
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcatgggcaa acaaacaaga tttgatcgct aaagtagcag aagctacaga
      421 attgactaag aaagactcag cagcagcagt tgaagctgta tttgcagcag tagctgacta
      481 tcttgcagct ggtgaaaaag ttcaattgat cggttttggt aactttgaag ttcgtgagcg
      541 tgcagaacgt aaaggtcgca acccacaaac tggtaaagaa atgacaattg cagcttctaa
      601 agtaccagca ttcaaagctg gtaaagctct taaagacgct gttaaaggat ccggatctgg
      661 tggagaagct gcagctaaag gatcagtctc tgaagaagat aatatggcta gtttgcctgc
      721 tacccacgaa ttacacattt ttggatcaat caatggagtc gattttgata tggttggtca
      781 aggaaccggt aatccaaatg atggatacga agaattgaat cttaagagta ctaagggtga
      841 tttacagttt tctccatgga ttttggtccc tcatatcgga tacggttttc accaatactt
      901 gccatatcct gatggaatgt ctccttttca ggctgcaatg gttgatggat caggttatca
      961 agtccatcgt acaatgcagt ttgaagatgg tgctagtctt accgttaatt atcgttacac
     1021 atatgaagga tctcacatta aaggtgaagc ccaagtaaag ggaacaggtt ttccagcaga
     1081 tggacctgtt atgactaatt cacttaccgc cgctgattgg tgtcgtagta aaaagactta
     1141 tccaaatgat aagacaatca tctctacttt taagtggtca tacacaactg gaaatggtaa
     1201 acgttatcgt tcaactgctc gtaccacata cacctttgca aaaccaatgg cagccaatta
     1261 ccttaagaat caacctatgt acgtctttcg taagacagaa cttaagcatt ctaagactga
     1321 acttaatttt aaggaatggc agaaggcttt tactgatgta atgggtatgg atgaattgta
     1381 taaataataa gaattaattc tatcctaatt gtggtttcaa aatcggctcc gtcgatacta
     1441 tgttatacgc caactttgaa aacaactttg aaaaagctgt tttctggtat ttaaggtttt
     1501 agaatgcaag gaacagtgaa ttggagttcg tcttgttata attagcttct tggggtatct
     1561 ttaaatactg tagaaaagag gaaggaaata ataaatggct aaaatgagaa tatcaccgga
     1621 attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat acggaaggaa tgtctcctgc
     1681 taaggtatat aagctggtgg gagaaaatga aaacctatat ttaaaaatga cggacagccg
     1741 gtataaaggg accacctatg atgtggaacg ggaaaaggac atgatgctat ggctggaagg
     1801 aaagctgcct gttccaaagg tcctgcactt tgaacggcat gatggctgga gcaatctgct
     1861 catgagtgag gccgatggcg tcctttgctc ggaagagtat gaagatgaac aaagccctga
     1921 aaagattatc gagctgtatg cggagtgcat caggctcttt cactccatcg acatatcgga
     1981 ttgtccctat acgaatagct tagacagccg cttagccgaa ttggattact tactgaataa
     2041 cgatctggcc gatgtggatt gcgaaaactg ggaagaagac actccattta aagatccgcg
     2101 cgagctgtat gattttttaa agacggaaaa gcccgaagag gaacttgtct tttcccacgg
     2161 cgacctggga gacagcaaca tctttgtgaa agatggcaaa gtaagtggct ttattgatct
     2221 tgggagaagc ggcagggcgg acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag
     2281 ggaggatatc ggggaagaac agtatgtcga gctatttttt gacttactgg ggatcaagcc
     2341 tgattgggag aaaataaaat attatatttt actggatgaa ttgttttagt acctaaatta
     2401 attctaatga gcactagtag gaggcatatc aaatgaactt taataaaatt gatttagaca
     2461 attggaagag aaaagatata tttaatcatt atttgaaccc aaacaaacga ctttgatatc
     2521 tttctagaag atctcctaca atattctcag ctgccatgga aaatcgatgt tcttctttta
     2581 ttctctcaag attttcaggc tgtatattaa aacttatatt aagaactatg ctaaccacct
     2641 catcaggaac cgttgtaggt ggcgtgggtt ttcttggcaa tcgactctca tgaaaactac
     2701 gagctaaata ttcaatatgt tcctcttgac caactttatt ctgcattttt tttgaacgag
     2761 gtttagagca agcttcagga aactgagaca ggaattttat taaaaattta aattttgaag
     2821 aaagttcagg gttaatagca tccatttttt gctttgcaag ttcctcagca ttcttaacaa
     2881 aagacgtctc ttttgacatg tttaaagttt aaacctcctg tgtgaaattg ttatccgctc
     2941 acaattccac acattatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     3001 gtgagctaac tcacattaat tgcgttgcgc tcactgccaa ttgctttcca gtcgggaaac
     3061 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3121 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3181 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3241 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3301 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3361 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3421 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3481 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3541 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3601 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3661 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3721 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     3781 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3841 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3901 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3961 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4021 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4081 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4141 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4201 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4261 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4321 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4381 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4441 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4501 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4561 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4621 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4681 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4741 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4801 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4861 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4921 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4981 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5041 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     5101 aataggcgta tcacgaggcc
//
