LOCUS       pJetScarlettEry         4688 bp    DNA     circular SYN 17-NOV-2021
DEFINITION  Cloning vector pJET1.2, complete sequence.
ACCESSION   EF694056
VERSION     EF694056.1
KEYWORDS    .
SOURCE      Cloning vector pJET1.2.
  ORGANISM  Cloning vector pJET1.2
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Bagdanaviciute,J., Zakareviciene,L. and Lubys,A.
  TITLE     Improved positive selection cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Lubys,A., Bagdanaviciute,J. and Zakareviciene,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2007) Research Center, Fermentas UAB, Graiciuno
            8, Vilnius LT-02241, Lithuania
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|-14135874|                                                 
            VNTDBDATE|-14135874|                                               
            LSOWNER|                                                           
            VNTNAME|pJetScarlettEry|                                           
            VNTAUTHORNAME|User|                                                
            VNTOAUTHORNAME|UNKNOWN|                                            
            Vector_NTI_Display_Data_(Do_Not_Edit!)                             
            (SXF                                                               
             (CGexDoc "pJetScarlettEry" 0 4688                                 
           
FEATURES             Location/Qualifiers
     CDS             complement(16..372)
                     /standard_name="'eco47I/T7"
     CDS             373..645
                     /standard_name="hlpA"
     CDS             658..684
                     /standard_name="Translation\596-622"
     misc_feature    664
                     /standard_name="G->T\in\mutant"
     CDS             685..1380
                     /standard_name="'mScarlet_Sp\CDS"
     CDS             1494..2231
                     /standard_name="ery"
     misc_feature    complement(2318..2337)
                     /standard_name="ermF"
     CDS             complement(2341..2467)
                     /standard_name="eco47I/T7"
     rep_origin      complement(2862..3476)
     gene            complement(3636..4496)
                     /gene="bla"
     CDS             complement(3636..4496)
                     /gene="bla"
                     /function="catalyzes the opening and hydrolysis of the
                     beta-lactam ring of beta-lactam antibiotics such as
                     penicillins and cephalosporins"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="ABR53873.1"
                     /standard_name="bla"
ORIGIN      
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tcatggcaaa caaacaagat ttgatcgcta aagtagcaga agctacagaa
      421 ttgactaaga aagactcagc agcagcagtt gaagctgtat ttgcagcagt agctgactat
      481 cttgcagctg gtgaaaaagt tcaattgatc ggttttggta actttgaagt tcgtgagcgt
      541 gcagaacgta aaggtcgcaa cccacaaact ggtaaagaaa tgacaattgc agcttctaaa
      601 gtaccagcat tcaaagctgg taaagctctt aaagacgctg ttaaaggatc cggatctggt
      661 ggagaagctg cagctaaagg atcagtgagt aaaggcgaag ctgtaataaa ggagttcatg
      721 agatttaaag tacacatgga ggggtcaatg aacggtcatg aatttgaaat tgagggagag
      781 ggagagggaa gaccgtatga gggtacgcaa accgccaaat tgaaggtaac gaagggggga
      841 cctcttcctt tttcatggga cattcttagc ccgcagttca tgtatgggag ccgagcattt
      901 attaagcacc cagcggacat accagattat tacaaacaat ctttccctga aggtttcaag
      961 tgggagagag ttatgaactt tgaggacgga ggagctgtaa cagtgaccca agatacttca
     1021 ctagaggacg ggactcttat ttacaaagtc aaactacgag gaacgaactt tccgcctgac
     1081 ggccctgtta tgcagaaaaa gactatgggc tgggaagcct ctacggagcg cctataccca
     1141 gaggatggtg ttttgaaagg tgacattaaa atggcacttc gcctaaagga cggaggccga
     1201 tacctagcag attttaaaac aacgtataaa gctaagaaac ctgtacaaat gccaggagca
     1261 tataatgttg atcgaaaatt agatattact tctcataacg aggactacac agtggttgaa
     1321 caatatgaac gtagcgaagg tcgtcattca actgggggca tggacgaact atataaataa
     1381 taagaattcc aaacttaaga gtgtgttgat agtgcagtat cttaaaattt tgtataatag
     1441 gaattgaagt taaattagat gctaaaaatt tgtaattaag aaggagtgat tacatgaaca
     1501 aaaatataaa atattctcaa aactttttaa cgagtgaaaa agtactcaac caaataataa
     1561 aacaattgaa tttaaaagaa accgataccg tttacgaaat tggaacaggt aaagggcatt
     1621 taacgacgaa actggctaaa ataagtaaac aggtaacgtc tattgaatta gacagtcatc
     1681 tattcaactt atcgtcagaa aaattaaaac tgaatactcg tgtcacttta attcaccaag
     1741 atattctaca gtttcaattc cctaacaaac agaggtataa aattgttggg agtattcctt
     1801 accatttaag cacacaaatt attaaaaaag tggtttttga aagccatgcg tctgacatct
     1861 atctgattgt tgaagaagga ttctacaagc gtaccttgga tattcaccga acactagggt
     1921 tgctcttgca cactcaagtc tcgattcagc aattgcttaa gctgccagcg gaatgctttc
     1981 atcctaaacc aaaagtaaac agtgtcttaa taaaacttac ccgccatacc acagatgttc
     2041 cagataaata ttggaagcta tatacgtact ttgtttcaaa atgggtcaat cgagaatatc
     2101 gtcaactgtt tactaaaaat cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt
     2161 taagtaccgt tacttatgag caagtattgt ctatttttaa tagttatcta ttatttaacg
     2221 ggaggaaata attctatgag tcgcttttgt aaatttggaa agttacacgt tactaaaggg
     2281 aatgtagata aattattagg tatactactg acagcttcca aggagctaaa gaggtccaag
     2341 cttcaggaaa ctgagacagg aattttatta aaaatttaaa ttttgaagaa agttcagggt
     2401 taatagcatc cattttttgc tttgcaagtt cctcagcatt cttaacaaaa gacgtctctt
     2461 ttgacatgtt taaagtttaa acctcctgtg tgaaattgtt atccgctcac aattccacac
     2521 attatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     2581 acattaattg cgttgcgctc actgccaatt gctttccagt cgggaaacct gtcgtgccag
     2641 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     2701 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     2761 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     2821 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     2881 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     2941 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     3001 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     3061 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     3121 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     3181 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     3241 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     3301 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     3361 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     3421 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     3481 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     3541 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     3601 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     3661 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     3721 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     3781 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     3841 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     3901 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     3961 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     4021 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     4081 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     4141 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     4201 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     4261 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     4321 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     4381 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     4441 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     4501 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     4561 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     4621 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     4681 acgaggcc
//
