LOCUS       pJetTurqCatQ            4942 bp    DNA     circular SYN 29-SEP-2021
DEFINITION  Cloning vector pJET1.2, complete sequence.
ACCESSION   EF694056
VERSION     EF694056.1
KEYWORDS    .
SOURCE      Cloning vector pJET1.2.
  ORGANISM  Cloning vector pJET1.2
            other sequences; artificial sequences; vectors.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Bagdanaviciute,J., Zakareviciene,L. and Lubys,A.
  TITLE     Improved positive selection cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Lubys,A., Bagdanaviciute,J. and Zakareviciene,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2007) Research Center, Fermentas UAB, Graiciuno
            8, Vilnius LT-02241, Lithuania
COMMENT     This file is created by Vector NTI                                 
            http://www.invitrogen.com/                                         
            ORIGDB|GenBank                                                     
            VNTDATE|-14045162|                                                 
            VNTDBDATE|-14065529|                                               
            LSOWNER|                                                           
            VNTNAME|pJetTurqCatQ|                                              
            VNTAUTHORNAME|User|                                                
            VNTOAUTHORNAME|UNKNOWN|                                            
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FEATURES             Location/Qualifiers
     CDS             complement(16..369)
                     /standard_name="'eco47I/T7"
     CDS             370..642
                     /standard_name="hlpA"
     CDS             655..681
                     /standard_name="Translation\287-313"
     misc_feature    668
                     /standard_name="C->A"
     misc_feature    682..1398
                     /standard_name="mTurquoise2"
     CDS             1625..2284
                     /db_xref="SEED:fig 6666666.94651.peg.298"
                     /db_xref="GO:0008811"
                     /product="Chloramphenicol acetyltransferase"
                     /EC_number="2.3.1.28"
                     /protein_id="F4-20_00579"
                     /standard_name="catQ"
     CDS             complement(2595..2721)
                     /standard_name="eco47I/T7'"
     rep_origin      complement(3116..3730)
     gene            complement(3890..4750)
                     /gene="bla"
     CDS             complement(3890..4750)
                     /gene="bla"
                     /function="catalyzes the opening and hydrolysis of the
                     beta-lactam ring of beta-lactam antibiotics such as
                     penicillins and cephalosporins"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="ABR53873.1"
                     /standard_name="bla"
ORIGIN      
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaca tggcaaacaa acaagatttg atcgctaaag tagcagaagc tacagaattg
      421 actaagaaag actcagcagc agcagttgaa gctgtatttg tagcagtagc tgactatctt
      481 gcagctggtg aaaaagttca attgatcggt tttggtaact ttgaagttcg tgagcgtgca
      541 gaacgtaaag gtcgcaaccc acaaactggt aaagaaatga caattgcagc ttctaaagta
      601 ccagcattca aagctggtaa agctcttaaa gacgctgtta aaggatccgg atctggtgga
      661 gaagctgaag ctaaaggatc agtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
      721 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
      781 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
      841 cccgtgccct ggcccaccct cgtgaccacc ctgtcctggg gcgtgcagtg cttcgcccgc
      901 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
      961 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     1021 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     1081 ggcaacatcc tggggcacaa gctggagtac aactacttca gcgacaacgt ctatatcacc
     1141 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
     1201 ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     1261 ctgctgcccg acaaccacta cctgagcacc cagtccaagc tgagcaaaga ccccaacgag
     1321 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     1381 gacgagctgt acaagtaata agaattccgt attggtggaa taacacgaat taataaagga
     1441 tatatttgta gtagcaagtg tatttgtttt atattctatg accctatttg atttacatga
     1501 ttctatatgg tataatggac ttgcttataa atatagatgc ttggatagca ggaggttgca
     1561 agatgatgat ggcagttaaa ttagccttct aaaattaaat aataaaattt taggaggcta
     1621 atttatgaaa tttaatttga tagatataga ggattggaat agaaagccat actttgagca
     1681 ttatttaaat gcggtaaggt gcacttacag tatgactgca aatttagaga taactagttt
     1741 actgcgtgaa attaaactta agggcctgaa actgtaccct acgcttattt atatcatcac
     1801 aactgtagtt aatcgtcaca aggagttccg caccagtttt gatcaaaacg gtaagttagg
     1861 atactgggat agtatgaacc caagttatac tatctttcat aaggataacg aaactttttc
     1921 aagtatttgg acagagtatg acgagaactt cccacgtttc tactataatt accttgagga
     1981 tatcagaaac tatagcgacg ttatgaattt catgcctaag gcaggtcaac ctgctaacac
     2041 aattaatgtg tccagcattc cttgggtgaa ttttaccgga ttcaacttga atatatataa
     2101 tgatgcaaca tatctaatcc ctatttttac tatgggtaag tattttcagc aggataataa
     2161 aattttatta cctatgtctg tacaggtgca tcatgcggtt tgcgacggtt accataccag
     2221 cagatttttt aatgaggtac aggaattagt ctcaaatttt gagacatggt taggagaaaa
     2281 ataatattaa aatgcttgtg ctcgggagtt ataaacaaag atcgaaaata ttgttaagat
     2341 cgtgaggaag caaagcgacc gaagaagtat atgttccatc tatgcaagga cttcccgaga
     2401 aggcgagtga cagcgaagac cgattggaag gagtctgagc taagtggagt aatatattca
     2461 ttcattatag aaccatcatt tcaaaggatt agagaaatct aatccttttt ctaggtattt
     2521 ttatcctaag aatttaatat gacaatcacg agtttaattt ttattttggt gctatttatc
     2581 ctaagcagag caagcttcag gaaactgaga caggaatttt attaaaaatt taaattttga
     2641 agaaagttca gggttaatag catccatttt ttgctttgca agttcctcag cattcttaac
     2701 aaaagacgtc tcttttgaca tgtttaaagt ttaaacctcc tgtgtgaaat tgttatccgc
     2761 tcacaattcc acacattata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     2821 gagtgagcta actcacatta attgcgttgc gctcactgcc aattgctttc cagtcgggaa
     2881 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     2941 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     3001 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     3061 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     3121 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     3181 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     3241 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     3301 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     3361 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     3421 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     3481 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     3541 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     3601 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     3661 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     3721 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     3781 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     3841 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3901 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3961 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     4021 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     4081 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     4141 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     4201 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     4261 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4321 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4381 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4441 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4501 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4561 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4621 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4681 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4741 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4801 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4861 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     4921 aaaataggcg tatcacgagg cc
//
