LOCUS       pBABE-ProDOL-seq        7474 bp DNA     circular SYN 06-SEP-2023
DEFINITION  pBABE-ProDOL.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 6, 2023 from SnapGene 6.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(496..1356)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  496 .. 1287 / #ccffcc
                        2: 1288 .. 1356 / #ccffcc / signal sequence
                     Cleavage site after base 1287"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1357..1461)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     LTR             1492..1961
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
                     /note="color: #ffe4c4"
     misc_feature    2026..2225
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
                     /note="color: #ffe4c4"
     misc_feature    2989..3705
                     /label=mT-Sapphire
                     /note="color: #290eee"
     CDS             2989..3705
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 2989 .. 2991 / #05fd14
                        2: 2992 .. 2994 / #05fd14 / 1a
                        3: 2995 .. 3705 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             3712..4599
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /note="color: #cc99b2"
                     /translation="AEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSY
                     VWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIH
                     DWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIID
                     QNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVE
                     EYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLI
                     GSEIARWLSTLEISG"
     CDS             4684..5229
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /note="color: #cc99b2"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             5236..5253
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     promoter        5293..5622
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5473..5608
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     CDS             5632..6231
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     LTR             6452..6921
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
                     /note="color: #ffe4c4"
     rep_origin      complement(join(7222..7474,1..336))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
       61 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      121 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      181 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      241 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      301 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      361 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
      421 aaaaggatct tcacctagat ccttttgcgg ccgcaaatca atctaaagta tatatgagta
      481 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
      541 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
      601 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
      661 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
      721 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
      781 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
      841 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
      901 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
      961 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     1021 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     1081 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     1141 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     1201 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     1261 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     1321 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     1381 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     1441 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcag cctgaatatg
     1501 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     1561 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag
     1621 ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc
     1681 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     1741 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     1801 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt
     1861 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     1921 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga
     1981 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggcaagct ggccagcaac
     2041 ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta
     2101 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttctgaac
     2161 acccggccgc aaccctggga gacgtcccag ggactttggg ggccgttttt gtggcccgac
     2221 ctgaggaagg gagtcgatgt ggaatccgac cccgtcagga tatgtggttc tggtaggaga
     2281 cgagaaccta aaacagttcc cgcctccgtc tgaatttttg ctttcggttt ggaaccgaag
     2341 ccgcgcgtct tgtctgctgc agcgctgcag catcgttctg tgttgtctct gtctgactgt
     2401 gtttctgtat ttgtctgaaa attagggcca gactgttacc actcccttaa gtttgacctt
     2461 aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg tcaagaagag
     2521 acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat ggccgcgaga
     2581 cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt cacctggccc
     2641 gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg cttttgaccc
     2701 ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc ctccatccgc
     2761 cccgtctctc ccccttgaac ctcctcgttc gaccccccct cgagctgcag agcttgctga
     2821 cccagctgtg gactagggga aaggggaaag aggaaaggga aggagaggtg gggggacgaa
     2881 acaggggcgc tggagccctg agcttcctga agctagggct gcatcgaatt catgggatgt
     2941 atcaagagta agcgtaagga taatctcaat gacgacgagc tcgagaccat ggtgagcaag
     3001 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
     3061 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
     3121 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     3181 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     3241 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     3301 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     3361 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     3421 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     3481 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     3541 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     3601 cagtccaaac tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     3661 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagggatc cgcagaaatc
     3721 ggtactggct ttccattcga cccccattat gtggaagtcc tgggcgagcg catgcactac
     3781 gtcgatgttg gtccgcgcga tggcacccct gtgctgttcc tgcacggtaa cccgacctcc
     3841 tcctacgtgt ggcgcaacat catcccgcat gttgcaccga cccatcgctg cattgctcca
     3901 gacctgatcg gtatgggcaa atccgacaaa ccagacctgg gttatttctt cgacgaccac
     3961 gtccgcttca tggatgcctt catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt
     4021 cacgactggg gctccgctct gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa
     4081 ggtattgcat ttatggagtt catccgccct atcccgacct gggacgaatg gccagaattt
     4141 gcccgcgaga ccttccaggc cttccgcacc accgacgtcg gccgcaagct gatcatcgat
     4201 cagaacgttt ttatcgaggg tacgctgccg atgggtgtcg tccgcccgct gactgaagtc
     4261 gagatggacc attaccgcga gccgttcctg aatcctgttg accgcgagcc actgtggcgc
     4321 ttcccaaacg agctgccaat cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa
     4381 tacatggact ggctgcacca gtcccctgtc ccgaagctgc tgttctgggg caccccaggc
     4441 gttctgatcc caccggccga agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct
     4501 gtggacatcg gcccgggtct gaatctgctg caagaagaca acccggacct gatcggcagc
     4561 gagatcgcgc gctggctgtc gacgctggag atttccggca ccggtttggc ggaggcggcg
     4621 gcgaaggagg cggcggcgaa ggaggcggcg gcgaaggagg cggcggcgaa ggcggcggcg
     4681 catatggaca aagactgcga aatgaagcgc accaccctgg atagccctct gggcaagctg
     4741 gaactgtctg ggtgcgaaca gggcctgcac cgtatcatct tcctgggcaa aggaacatct
     4801 gccgccgacg ccgtggaagt gcctgcccca gccgccgtgc tgggcggacc agagccactg
     4861 atgcaggcca ccgcctggct caacgcctac tttcaccagc ctgaggccat cgaggagttc
     4921 cctgtgccag ccctgcacca cccagtgttc cagcaggaga gctttacccg ccaggtgctg
     4981 tggaaactgc tgaaagtggt gaagttcgga gaggtcatca gctacagcca cctggccgcc
     5041 ctggccggca atcccgccgc caccgccgcc gtgaaaaccg ccctgagcgg aaatcccgtg
     5101 cccattctga tcccctgcca ccgggtggtg cagggcgacc tggacgtggg gggctacgag
     5161 ggcgggctcg ccgtgaaaga gtggctgctg gcccacgagg gccacagact gggcaagcct
     5221 gggctgggtc aattgcacca tcaccatcac cactaattaa ttaagccagc acagtggtcg
     5281 accctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5341 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5401 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5461 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5521 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     5581 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac catgaccgag
     5641 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     5701 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     5761 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     5821 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     5881 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     5941 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     6001 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     6061 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     6121 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     6181 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
     6241 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgat aaaataaaag
     6301 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     6361 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag
     6421 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg
     6481 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca
     6541 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc
     6601 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc
     6661 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
     6721 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc
     6781 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
     6841 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
     6901 cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa ttaatttggt tttttttctt
     6961 aagtatttac attaaatggc catagttgca ttaatgaatc ggccaacgcg cggggagagg
     7021 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     7081 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     7141 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     7201 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     7261 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     7321 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     7381 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     7441 ttcggtgtag gtcgttcgct ccaagctggg ctgt
//
