LOCUS       Exported                8695 bp ds-DNA     circular SYN 27-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Murray Junop
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Oct 27, 2023 from SnapGene Viewer 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8695
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     primer_bind     2..21
                     /label=MJ3829
                     /note="T7 Promoter"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    21..45
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     44..63
                     /label=MJ8283
                     /note="pet-Duet1 MCS1 Sequencing primer -fwd"
     primer_bind     45..72
                     /label=MJ8002
                     /note="pCOLA-MCS-1-F"
     RBS             60..82
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             94..170
                     /codon_start=1
                     /label=Super-His
                     /translation="SKHHHHSGHHHTGHHHHSGSHHHSG"
     CDS             177..545
                     /codon_start=1
                     /product="Monomeric OCR (mOCR) solubility tag.doi: 
                     10.1016/j.pep.2008.08.011
                     "
                     /label=mOCR
                     /translation="SSGVDLAMSNMTYNNVFDHAYEMLKENIRYDDIRDTDDLHDAIHM
                     AADNAVPHYYADIRSVMASEGIDLEFEDSGLMPDTKDDIRILQARIYEQLTIDLWEDAE
                     DLLNEYLEEVEEYEEDEEG"
     protein_bind    564..688
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        713..743
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             797..1456
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(997..1015)
                     /label=MJ1011
                     /note="pLysS seq primer"
     primer_bind     1282..1301
                     /label=MJ1028
                     /note="pLys seq primer"
     primer_bind     1297..1308
                     /label=MJ8252
                     /note="insA-Pso2FL-R (Revert a cloning error)"
     primer_bind     complement(1333..1351)
                     /label=MJ1024
                     /note="pLys seq primer"
     primer_bind     complement(1440..1452)
                     /label=MJ3173
                     /note="K186A Reverse"
     primer_bind     1462..1476
                     /label=MJ3798
                     /note="Arm2"
     rep_origin      1509..2097
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1780..1802)
                     /label=MJ3986
                     /note="pFQ-5OH-D"
     primer_bind     1964..1983
                     /label=MJ3281
                     /note="Reverse primer for generation of 1000bp dsDNA 
                     substrate with blunt ends (puc19 plasmid)"
     CDS             2730..3035
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3076..3200)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     3179..3199
                     /label=MJ3894
                     /note="210_NLS-Fwd"
     CDS             3260..3292
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             3299..3316
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3396..3417)
                     /label=MJ3828
                     /note="rrnB term Rev"
     primer_bind     3474..3499
                     /label=MJ3801
                     /note="EXDA3"
     terminator      3482..3568
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3660..3687
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     3671..3695
                     /label=MJ3954
                     /note="Amp Fwd - for MJ4602"
     primer_bind     complement(3741..3759)
                     /label=MJ3830
                     /note="T7 Terminator"
     terminator      3755..3802
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      3839..4294
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3866..3877)
                     /label=MJ3665
                     /note="A31K/A32K Reverse Bg1II"
     CDS             complement(4409..5269)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4438..4457
                     /label=MJ3985
                     /note="pFQ-5OH-C"
     primer_bind     complement(4438..4457)
                     /label=MJ3984
                     /note="pFQ-***-F-B"
     promoter        complement(5270..5362)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5369..5410
                     /label=MJ3798
                     /note="Arm2"
     rep_origin      5443..6031
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5714..5736)
                     /label=MJ3986
                     /note="pFQ-5OH-D"
     primer_bind     5898..5917
                     /label=MJ3281
                     /note="Reverse primer for generation of 1000bp dsDNA 
                     substrate with blunt ends (puc19 plasmid)"
     misc_feature    6217..6359
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(6461..6652)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6476..6495)
                     /label=MJ3280
                     /note="Forward primer for generation of 1000bp dsDNA 
                     substrate with blunt ends (puc19 plasmid)"
     primer_bind     complement(7144..7162)
                     /label=MJ1019
                     /note="pLys seq primer"
     CDS             complement(7224..8306)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     7368..7379
                     /label=MJ3173
                     /note="K186A Reverse"
     primer_bind     8201..8210
                     /label=MJ8252
                     /note="insA-Pso2FL-R (Revert a cloning error)"
     promoter        complement(8307..8384)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     join(8678..8695,1..22)
                     /label=MJ1079
                     /note="PCR cloning primer for up stream promoter region"
     primer_bind     join(8678..8695,1..22)
                     /label=MJ1034
                     /note="PCR primer for cloning full length XRcc4 into 
                     pJ4039"
ORIGIN
        1 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
       61 ttgtttaact ttaagaagga gatatacata tgagatctaa gcaccaccac cacagcggtc
      121 atcatcacac cggccatcat caccacagcg gtagccacca ccacagcggt accggtagca
      181 gcggcgtgga cctggcgatg agcaacatga cctacaacaa cgttttcgat cacgcgtatg
      241 agatgctgaa agaaaacatc cgttacgacg atattcgtga caccgacgat ctgcacgatg
      301 cgattcatat ggcggcggac aacgcggtgc cgcactacta tgcggatatc cgtagcgtta
      361 tggcgagcga gggtattgac ctggagtttg aagatagcgg cctgatgccg gacaccaagg
      421 acgatatccg tattctgcag gcgcgtatct atgagcaact gaccattgat ctgtgggagg
      481 acgcggagga cctgctgaac gaatacctgg aggaagtgga ggaatatgag gaagacgagg
      541 aaggtaccgg tagatctgat atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa
      601 atgatataaa tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact
      661 gtaaaacaca acatatccag tcactatggc ggccgcatta ggcaccccag gctttacact
      721 ttatgcttcc ggctcgtata atgtgtggat tttgagttag gatccggcga gattttcagg
      781 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
      841 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
      901 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
      961 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     1021 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     1081 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     1141 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     1201 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     1261 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     1321 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     1381 ctgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     1441 gcagggcggg gcgtaaacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1501 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1561 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1621 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     1681 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1741 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1801 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1861 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1921 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1981 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2041 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2101 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2161 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2221 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     2281 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     2341 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatac gcgtggatcc
     2401 ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     2461 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtgtgctatg aagcagcgta
     2521 ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg atgtcaatat
     2581 ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg
     2641 gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa tgaacggctc
     2701 ttttgctgac gagaacaggg actggtgaaa tgcagtttaa ggtttacacc tataaaagag
     2761 agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg cccgggcgac
     2821 ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt
     2881 acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat atggccagtg
     2941 tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca
     3001 aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccgtt atacacagcc
     3061 agtctgcagg tcgaccatag tgactggata tgttgtgttt tacagtatta tgtagtctgt
     3121 tttttatgca aaatctaatt taatatattg atatttatat cattttacgt ttctcgttca
     3181 gctttcttgt acaaagtggt gatatcgcta gctagatagc tagaagcttg cggccgcaca
     3241 gctgtataca cgtgcaagcc agccagaact cgctcctgaa gacccagagg atctcgagca
     3301 ccaccaccac caccactaat gttaattaag ttgggcgttc ctaggctgat aaaacagaat
     3361 ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa
     3421 cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca
     3481 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     3541 ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca
     3601 acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca
     3661 gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct
     3721 aaatacattc aaatatgtat ccgctgagca ataactagca taaccccttg gggcctctaa
     3781 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac
     3841 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3901 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3961 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     4021 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     4081 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     4141 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     4201 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     4261 gcgaatttta acaaaatatt aacgtttaca atttctggcg gcacgatggc atgagattat
     4321 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     4381 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     4441 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     4501 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     4561 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     4621 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     4681 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     4741 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     4801 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     4861 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     4921 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     4981 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5041 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5101 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     5161 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     5221 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     5281 ttcaatcatg attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     5341 tgtatttaga aaaataaaca aataggtcat gaccaaaatc ccttaacgtg agttttcgtt
     5401 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     5461 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     5521 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     5581 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     5641 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     5701 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     5761 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     5821 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     5881 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     5941 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6001 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6061 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     6121 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     6181 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     6241 gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat
     6301 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     6361 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     6421 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     6481 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac
     6541 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     6601 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
     6661 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
     6721 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
     6781 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
     6841 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
     6901 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
     6961 cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
     7021 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
     7081 gggtcctcaa cgacaggagc acgatcatgc tagtcatgcc ccgcgcccac cggaaggagc
     7141 tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct
     7201 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     7261 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
     7321 gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac cgcctggccc
     7381 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
     7441 atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta tcccactacc
     7501 gagatgtccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
     7561 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
     7621 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
     7681 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt
     7741 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc
     7801 agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca
     7861 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca
     7921 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc
     7981 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt
     8041 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg
     8101 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg
     8161 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg
     8221 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg
     8281 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
     8341 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc
     8401 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     8461 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     8521 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     8581 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     8641 gccggccacg atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaa
//
