LOCUS       pUltra-hot-mCherry-BLM 12424 bp ds-DNA     circular     03-AUG-2021
DEFINITION  .
KEYWORDS    "marker:AmpR"
COMMENT     3rd generation Lentiviral vector for bi-cistronic expression of
            mCherry and the gene of interest. <small>[1] pUltra series</small>
FEATURES             Location/Qualifiers
     LTR             236..416
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    460..585
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1078..1311
                     /label="RRE"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    1838..1955
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        2020..3231
                     /label="UbC promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          3069..3090
                     /label="UbCpro1-frwd"
                     /note="sequence: gaagctccggttttgaactatg"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             3263..3970
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff0000"
                     /ApEinfo_fwdcolor="#ff0000"
     CDS             3263..3970
                     /label="Translation 3263-3970"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK"
     primer          3901..3920
                     /label="mcherry-frwd- colonyPCR"
                     /note="sequence: caccatcgtggaacagtacg"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          3941..3995
                     /label="YD61: mCherry-BLM frwd"
                     /note="sequence: tccaccggcggcatggacgagctgtacaagGCTGCTGTTCCTCAAAATAATCTAC"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    3971..8218
                     /label="BLM wt"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             3971..8221
                     /label="Translation 3971-8221"
                     /translation="AAVPQNNLQEQLERHSARTLNNKLSLSKPKFSGFTFKKKTSSDNNVSVTNVSVAKTPVLRNKDVNVTEDFSFSEPLPNTTNQQRVKDFFKNAPAGQETQRGGSKSLLPDFLQTPKEVVCTTQNTPTVKKSRDTALKKLEFSSSPDSLSTINDWDDMDDFDTSETSKSFVTPPQSHFVRVSTAQKSKKGKRNFFKAQLYTTNTVKTDLPPPSSESEQIDLTEEQKDDSEWLSSDVICIDDGPIAEVHINEDAQESDSLKTHLEDERDNSEKKKNLEEAELHSTEKVPCIEFDDDDYDTDFVPPSPEEIISASSSSSKCLSTLKDLDTSDRKEDVLSTSKDLLSKPEKMSMQELNPETSTDCDARQISLQQQLIHVMEHICKLIDTIPDDKLKLLDCGNELLQQRNIRRKLLTEVDFNKSDASLLGSLWRYRPDSLDGPMEGDSCPTGNSMKELNFSHLPSNSVSPGDCLLTTTLGKTGFSATRKNLFERPLFNTHLQKSFVSSNWAETPRLGKKNESSYFPGNVLTSTAVKDQNKHTASINDLERETQPSYDIDNFDIDDFDDDDDWEDIMHNLAASKSSTAAYQPIKEGRPIKSVSERLSSAKTDCLPVSSTAQNINFSESIQNYTDKSAQNLASRNLKHERFQSLSFPHTKEMMKIFHKKFGLHNFRTNQLEAINAALLGEDCFILMPTGGGKSLCYQLPACVSPGVTVVISPLRSLIVDQVQKLTSLDIPATYLTGDKTDSEATNIYLQLSKKDPIIKLLYVTPEKICASNRLISTLENLYERKLLARFVIDEAHCVSQWGHDFRQDYKRMNMLRQKFPSVPVMALTATANPRVQKDILTQLKILRPQVFSMSFNRHNLKYYVLPKKPKKVAFDCLEWIRKHHPYDSGIIYCLSRRECDTMADTLQRDGLAALAYHAGLSDSARDEVQQKWINQDGCQVICATIAFGMGIDKPDVRFVIHASLPKSVEGYYQESGRAGRDGEISHCLLFYTYHDVTRLKRLIMMEKDGNHHTRETHFNNLYSMVHYCENITECRRIQLLAYFGENGFNPDFCKKHPDVSCDNCCKTKDYKTRDVTDDVKSIVRFVQEHSSSQGMRNIKHVGPSGRFTMNMLVDIFLGSKSAKIQSGIFGKGSAYSRHNAERLFKKLILDKILDEDLYINANDQAIAYVMLGNKAQTVLNGNLKVDFMETENSSSVKKQKALVAKVSQREEMVKKCLGELTEVCKSLGKVFGVHYFNIFNTVTLKKLAESLSSDPEVLLQIDGVTEDKLEKYGAEVISVLQKYSEWTSPAEDSSPGISLSSSRGPGRSAAEELDEEIPVSSHYFASKTRNERKRKKMPASQRSKRRKTASSGSKAKGGSATCRKISSKTKSSSIIGSSSASHTSQATSGANSKLGIMAPPKPINRPFLKPSYAFS*"
     primer          complement(4120..4142)
                     /label="BLM-R1 colony PCR"
                     /note="sequence: CAGGTGTTTTTGCTACTGACACA"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(8196..8251)
                     /label="YD62: UltHot-BLM rev"
                     /note="sequence: ctccggaacgaattctgatcacacgctagcTTATGAGAATGCATATGAAGGCTTAA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     misc_feature    8219..8221
                     /label="stop"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    8222..8251
                     /label="Right Homology"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(8258..8279)
                     /label="pUltH2-rev"
                     /note="sequence: atccagaggttgattgtcgagt"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    8265..8853
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          complement(8415..8436)
                     /label="pUltH1-rev"
                     /note="sequence: cataaagagacagcaaccagga"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(8736..8747)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     LTR             8944..9177
                     /label="3' LTR (ΔU3)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     polyA_signal    9249..9370
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      9410..9545
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        complement(9566..9584)
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     complement(9594..9610)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     rep_origin      9755..10210
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        10239..10343
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             10344..10412
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             10413..11204
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      11375..11963
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     protein_bind    12251..12272
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     promoter        12287..12292
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        12293..12310
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     promoter        12311..12317
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     protein_bind    12325..12341
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
     primer_bind     12349..12365
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
     promoter        12386..12404
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
ORIGIN
        1 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
       61 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      121 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      181 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacaa taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc
     1801 gccaattctg cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     1861 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     1921 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     1981 atccagtttg gttaattaac ccgtgtcggc tccagatctg gcctccgcgc cgggttttgg
     2041 cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca
     2101 gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact
     2161 cggccttaga accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta
     2221 gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg
     2281 attctgcgga gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg
     2341 gtgtggcaca gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc
     2401 gctgtgatcg tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc
     2461 cgggccgctc ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc
     2521 gcgagcaagg ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc
     2581 cgagtcttga atggaagacg cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg
     2641 ggcatggtgg gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt
     2701 attcgggtga gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac
     2761 tggagaactc ggtttgtcgt ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag
     2821 tgcacccgta cctttgggag cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg
     2881 gcttataatg cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc
     2941 aggacgcagg gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg
     3001 tgaggggagg gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt
     3061 aagtagctga agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa
     3121 gttttttagg caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag
     3181 actagtaaat tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg
     3241 gctgcaggtc cgatccaccg gtatggtgag caagggcgag gaggataaca tggccatcat
     3301 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
     3361 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
     3421 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
     3481 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
     3541 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
     3601 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
     3661 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
     3721 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
     3781 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
     3841 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
     3901 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
     3961 gctgtacaag GCTGCTGTTC CTCAAAATAA TCTACAGGAG CAACTAGAAC GTCACTCAGC
     4021 CAGAACACTT AATAATAAAT TAAGTCTTTC AAAACCAAAA TTTTCAGGTT TCACTTTTAA
     4081 AAAGAAAACA TCTTCAGATA ACAATGTATC TGTAACTAAT GTGTCAGTAG CAAAAACACC
     4141 TGTATTAAGA AATAAAGATG TTAATGTTAC CGAAGACTTT TCCTTCAGTG AACCTCTACC
     4201 CAACACCACA AATCAGCAAA GGGTCAAGGA CTTCTTTAAA AATGCTCCAG CAGGACAGGA
     4261 AACACAGAGA GGTGGATCAA AATCATTATT GCCAGATTTC TTGCAGACTC CGAAGGAAGT
     4321 TGTATGCACT ACCCAAAACA CACCAACTGT AAAGAAATCC CGGGATACTG CTCTCAAGAA
     4381 ATTAGAATTT AGTTCTTCAC CAGATTCTTT AAGTACCATC AATGATTGGG ATGATATGGA
     4441 TGACTTTGAT ACTTCTGAGA CTTCAAAATC ATTTGTTACA CCACCCCAAA GTCACTTTGT
     4501 AAGAGTAAGC ACTGCTCAGA AATCAAAAAA GGGTAAGAGA AACTTTTTTA AAGCACAGCT
     4561 TTATACAACA AACACAGTAA AGACTGATTT GCCTCCACCC TCCTCTGAAA GCGAGCAAAT
     4621 AGATTTGACT GAGGAACAGA AGGATGACTC AGAATGGTTA AGCAGCGATG TGATTTGCAT
     4681 CGATGATGGC CCCATTGCTG AAGTGCATAT AAATGAAGAT GCTCAGGAAA GTGACTCTCT
     4741 GAAAACTCAT TTGGAAGATG AAAGAGATAA TAGCGAAAAG AAGAAGAATT TGGAAGAAGC
     4801 TGAATTACAT TCAACTGAGA AAGTTCCATG TATTGAATTT GATGATGATG ATTATGATAC
     4861 GGATTTTGTT CCACCTTCTC CAGAAGAAAT TATTTCTGCT TCTTCTTCCT CTTCAAAATG
     4921 CCTTAGTACG TTAAAGGACC TTGACACATC TGACAGAAAA GAGGATGTTC TTAGCACATC
     4981 AAAAGATCTT TTGTCAAAAC CTGAGAAAAT GAGTATGCAG GAGCTGAATC CAGAAACCAG
     5041 CACAGACTGT GACGCTAGAC AGATAAGTTT ACAGCAGCAG CTTATTCATG TGATGGAGCA
     5101 CATCTGTAAA TTAATTGATA CTATTCCTGA TGATAAACTG AAACTTTTGG ATTGTGGGAA
     5161 CGAACTGCTT CAGCAGCGGA ACATAAGAAG GAAACTTCTA ACGGAAGTAG ATTTTAATAA
     5221 AAGTGATGCC AGTCTTCTTG GCTCATTGTG GAGATACAGG CCTGATTCAC TTGATGGCCC
     5281 TATGGAGGGT GATTCCTGCC CTACAGGGAA TTCTATGAAG GAGTTAAATT TTTCACACCT
     5341 TCCCTCAAAT TCTGTTTCTC CTGGGGACTG TTTACTGACT ACCACCCTAG GAAAGACAGG
     5401 ATTCTCTGCC ACCAGGAAGA ATCTTTTTGA AAGGCCTTTA TTCAATACCC ATTTACAGAA
     5461 GTCCTTTGTA AGTAGCAACT GGGCTGAAAC ACCAAGACTA GGAAAAAAAA ATGAAAGCTC
     5521 TTATTTCCCA GGAAATGTTC TCACAAGCAC TGCTGTGAAA GATCAGAATA AACATACTGC
     5581 TTCAATAAAT GACTTAGAAA GAGAAACCCA ACCTTCCTAT GATATTGATA ATTTTGACAT
     5641 AGATGACTTT GATGATGATG ATGACTGGGA AGACATAATG CATAATTTAG CAGCCAGCAA
     5701 ATCTTCCACA GCTGCCTATC AACCCATCAA GGAAGGTCGG CCAATTAAAT CAGTATCAGA
     5761 AAGACTTTCC TCAGCCAAGA CAGACTGTCT TCCAGTGTCA TCTACTGCTC AAAATATAAA
     5821 CTTCTCAGAG TCAATTCAGA ATTATACTGA CAAGTCAGCA CAAAATTTAG CATCCAGAAA
     5881 TCTGAAACAT GAGCGTTTCC AAAGTCTTAG TTTTCCTCAT ACAAAGGAAA TGATGAAGAT
     5941 TTTTCATAAA AAATTTGGCC TGCATAATTT TAGAACTAAT CAGCTAGAGG CGATCAATGC
     6001 TGCACTGCTT GGTGAAGACT GTTTTATCCT GATGCCGACT GGAGGTGGTA AGAGTTTGTG
     6061 TTACCAGCTC CCTGCCTGTG TTTCTCCTGG GGTCACTGTT GTCATTTCTC CCTTGAGATC
     6121 ACTTATCGTA GATCAAGTCC AAAAGCTGAC TTCCTTGGAT ATTCCAGCTA CATATCTGAC
     6181 AGGTGATAAG ACTGACTCAG AAGCTACAAA TATTTACCTC CAGTTATCAA AAAAAGACCC
     6241 AATCATAAAA CTTCTATATG TCACTCCAGA AAAGATCTGT GCAAGTAACA GACTCATTTC
     6301 TACTCTGGAG AATCTCTATG AGAGGAAGCT CTTGGCACGT TTTGTTATTG ATGAAGCACA
     6361 TTGTGTCAGT CAGTGGGGAC ATGATTTTCG TCAAGATTAC AAAAGAATGA ATATGCTTCG
     6421 CCAGAAGTTT CCTTCTGTTC CGGTGATGGC TCTTACGGCC ACAGCTAATC CCAGGGTACA
     6481 GAAGGACATC CTGACTCAGC TGAAGATTCT CAGACCTCAG GTGTTTAGCA TGAGCTTTAA
     6541 CAGACATAAT CTGAAATACT ATGTATTACC GAAAAAGCCT AAAAAGGTGG CATTTGATTG
     6601 CCTAGAATGG ATCAGAAAGC ACCACCCATA TGATTCAGGG ATAATTTACT GCCTCTCCAG
     6661 GCGAGAATGT GACACCATGG CTGACACGTT ACAGAGAGAT GGGCTCGCTG CTCTTGCTTA
     6721 CCATGCTGGC CTCAGTGATT CTGCCAGAGA TGAAGTGCAG CAGAAGTGGA TTAATCAGGA
     6781 TGGCTGTCAG GTTATCTGTG CTACAATTGC ATTTGGAATG GGGATTGACA AACCGGACGT
     6841 GCGATTTGTG ATTCATGCAT CTCTCCCTAA ATCTGTGGAG GGTTACTACC AAGAATCTGG
     6901 CAGAGCTGGA AGAGATGGGG AAATATCTCA CTGCCTGCTT TTCTATACCT ATCATGATGT
     6961 GACCAGACTG AAAAGACTTA TAATGATGGA AAAAGATGGA AACCATCATA CAAGAGAAAC
     7021 TCACTTCAAT AATTTGTATA GCATGGTACA TTACTGTGAA AATATAACGG AATGCAGGAG
     7081 AATACAGCTT TTGGCCTACT TTGGTGAAAA TGGATTTAAT CCTGATTTTT GTAAGAAACA
     7141 CCCAGATGTT TCTTGTGATA ATTGCTGTAA AACAAAGGAT TATAAAACAA GAGATGTGAC
     7201 TGACGATGTG AAAAGTATTG TAAGATTTGT TCAAGAACAT AGTTCATCAC AAGGAATGAG
     7261 AAATATAAAA CATGTAGGTC CTTCTGGAAG ATTTACTATG AATATGCTGG TCGACATTTT
     7321 CTTGGGGAGT AAGAGTGCAA AAATCCAGTC AGGTATATTT GGAAAAGGAT CTGCTTATTC
     7381 ACGACACAAT GCCGAAAGAC TTTTTAAAAA GCTGATACTT GACAAGATTT TGGATGAAGA
     7441 CTTATATATC AATGCCAATG ACCAGGCGAT CGCTTATGTG ATGCTCGGAA ATAAAGCCCA
     7501 AACTGTACTA AATGGCAATT TAAAGGTAGA CTTTATGGAA ACAGAAAATT CCAGCAGTGT
     7561 GAAAAAACAA AAAGCGTTAG TAGCAAAAGT GTCTCAGAGG GAAGAGATGG TTAAAAAATG
     7621 TCTTGGAGAA CTTACAGAAG TCTGCAAATC TCTGGGGAAA GTTTTTGGTG TCCATTACTT
     7681 CAATATTTTT AATACCGTCA CTCTCAAGAA GCTTGCAGAA TCTTTATCTT CTGATCCTGA
     7741 GGTTTTGCTT CAAATTGATG GTGTTACTGA AGACAAACTG GAAAAATATG GTGCGGAAGT
     7801 GATTTCAGTA TTACAGAAAT ACTCTGAATG GACATCGCCA GCTGAAGACA GTTCCCCAGG
     7861 GATAAGCCTG TCCAGCAGCA GAGGCCCCGG AAGAAGTGCC GCTGAGGAGC TCGACGAGGA
     7921 AATACCTGTA TCTTCCCACT ACTTTGCAAG TAAAACCAGA AATGAAAGGA AGAGGAAAAA
     7981 GATGCCAGCC TCCCAAAGGT CTAAGAGGAG AAAAACTGCT TCCAGTGGTT CCAAGGCAAA
     8041 GGGGGGGTCT GCCACATGTA GAAAGATATC TTCCAAAACG AAATCCTCCA GCATCATTGG
     8101 ATCCAGTTCA GCCTCACATA CTTCTCAAGC GACATCAGGA GCCAATAGCA AATTGGGGAT
     8161 TATGGCTCCA CCGAAGCCTA TAAATAGACC GTTTCTTAAG CCTTCATATG CATTCTCATA
     8221 Agctagcgtg tgatcagaat tcgttccgga gtcgtcgact cgacaatcaa cctctggatt
     8281 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     8341 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     8401 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     8461 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     8521 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     8581 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     8641 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     8701 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     8761 cttcccgcgg cctgctgccg gctctgcggc ctcttccgca tcttcgcctt cgccctcaga
     8821 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattcgagc tcggtacctt
     8881 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8941 gactggaagg gctacgtaac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     9001 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     9061 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     9121 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     9181 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     9241 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9301 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9361 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     9421 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9481 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9541 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     9601 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     9661 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     9721 caacagttgc gcagcctgaa tggcgaatgg cgcgacgcgc cctgtagcgg cgcattaagc
     9781 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     9841 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     9901 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     9961 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
    10021 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
    10081 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
    10141 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
    10201 tttacaattt cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
    10261 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
    10321 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
    10381 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
    10441 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
    10501 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
    10561 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
    10621 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
    10681 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
    10741 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
    10801 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    10861 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    10921 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    10981 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    11041 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    11101 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    11161 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    11221 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    11281 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    11341 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    11401 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    11461 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    11521 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    11581 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    11641 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    11701 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    11761 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    11821 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    11881 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    11941 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    12001 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    12061 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    12121 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    12181 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    12241 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    12301 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    12361 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    12421 tgca
//