LOCUS       pAAV-CMV-ABE8e-SpGN_aa7 7172 bp ds-DNA     circular     14-MAR-2023
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            addgene-plasmid-80930-sequence-152874.gbk Expresses Cas9N in
            mammalian cells..
FEATURES             Location/Qualifiers
     primer_bind     143..162
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     396..413
                     /label="L4440"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    530..551
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        566..596
                     /label="lac promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     protein_bind    604..620
                     /label="lac operator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer_bind     609..631
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     628..644
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     primer_bind     628..644
                     /label="M13 rev"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     repeat_region   671..800
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(848..868)
                     /label="pBABE 3'"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    896..915
                     /label="overlap"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     enhancer        916..1295
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1279..1303
                     /label="N-qPCR-titer-F"
                     /note="sequence: tcatcgctattaccatggtgatgcg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        1296..1499
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     1450..1470
                     /label="CMV-F"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1500..1515
                     /label="overlap"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          1506..1559
                     /label="7.10-8e构建上游引物"
                     /note="sequence: aagccgctagcgctaccggtcgccaccatggccccaaagaagaagcggaaagtc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     regulatory      1527..1536
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1533..1538
                     /label="Translation 1533-1538"
                     /translation="MA"
     primer          complement(1536..1560)
                     /label="N-qPCR-titer-R"
                     /note="sequence: agactttccgcttcttctttggggc"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             1539..1559
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             1539..4160
                     /label="Translation 1539-4160"
                     /translation="PKKKRKVSEVEFSHEYWMRHALTLAKRARDEREVPVGAVLVLNNRVIGEGWNRAIGLHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTFEPCVMCAGAMIHSRIGRVVFGVRNSKRGAAGSLMNVLNYPGMNHRVEITEGILADECAALLCDFYRMPRQVFNAQKKAQSSINSGGSSGGSSGSETPGTSESATPESSGGSSGGSDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGI"
     misc_feature    1560..2057
                     /label="ABE8e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1886..1905
                     /label="测序引物-2"
                     /note="sequence: aagaggcgccgcaggctccc"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    2058..2153
                     /label="Linker"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    2154..4160
                     /label="SpG N"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    2154..4289
                     /label="SpG N aa713"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2178..2180
                     /label="D10A Nickase"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     primer          2267..2286
                     /label="QPCR-SpG-N-F1"
                     /note="sequence: ccggcacagcatcaagaaga"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(2386..2422)
                     /label="7.10-8e构建下游引物"
                     /note="sequence: gccatctcgttgctgaagatctcttgcagatagcaga"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          complement(2394..2415)
                     /label="QPCR-SpG-N-R1"
                     /note="sequence: cgttgctgaagatctcttgcag"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          2881..2900
                     /label="Seq3"
                     /note="sequence: tgagcctgggcctgaccccc"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          2935..2955
                     /label="QPCR-SpG-N-F2"
                     /note="sequence: ccaaactgcagctgagcaagg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(3068..3089)
                     /label="QPCR-SpG-N-R2"
                     /note="sequence: ggtgatctcggtgttcactctc"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          3583..3602
                     /label="Seq4"
                     /note="sequence: tcgaggaagtggtggacaag"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4161..4177
                     /label="overlap"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4161..4289
                     /label="Cas9 N-term"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     CDS             4161..4595
                     /label="Translation 4161-4595"
                     /translation="RDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVCLAGDTLITLADGRRVPIRELVSQQNFSVWALNPQTYRLERARVSRAFCTGIKPVYRLTTRLGRSIRATANHRFLTPQGWKRVDELQPGDYLALPRRIPTAS"
     primer          4248..4267
                     /label="Seq5"
                     /note="sequence: gactctctcacctttaagga"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    4290..4595
                     /label="Rma Intein-N"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             4596..4598
                     /label="Translation 4596-4598"
                     /translation="*"
     primer_bind     complement(4609..4628)
                     /label="EBV-rev"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     polyA_signal    4638..4719
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     4663..4682
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     repeat_region   4902..5031
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     complement(5050..5066)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(5050..5067)
                     /label="M13 Forward"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(5059..5081)
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          5060..5079
                     /label="Seq6"
                     /note="sequence: gttttacaacgtcgtgactg"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     rep_origin      5208..5663
                     /label="f1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(5295..5314)
                     /label="F1ori-R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     5505..5526
                     /label="F1ori-F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     promoter        5690..5794
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             5795..6655
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          5919..5938
                     /label="Seq7"
                     /note="sequence: tgggttacatcgaactggat"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(6013..6032)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          6658..6677
                     /label="Seq8"
                     /note="sequence: gtcagaccaagtttactcat"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     rep_origin      6826..242
                     /label="ori"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
ORIGIN
        1 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
       61 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      121 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      181 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      241 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctgcggt tttgctcaca
      301 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      361 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      421 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
      481 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
      541 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
      601 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccagat
      661 ttaattaagg ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg
      721 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa
      781 ctccatcact aggggttcct tgtagttaat gattaacccg ccatgctact tatctacctg
      841 cggccgcgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
      901 agtatgcaaa gcatggacat tgattattga ctagttatta atagtaatca attacggggt
      961 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     1021 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     1081 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     1141 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     1201 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     1261 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     1321 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     1381 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     1441 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg
     1501 taccgaagcc gctagcgcta ccggtcgcca ccatggcccc aaagaagaag cggaaagtct
     1561 ctgaggtgga gttttcccac gagtactgga tgagacatgc cctgaccctg gccaagaggg
     1621 cacgggatga gagggaggtg cctgtgggag ccgtgctggt gctgaacaat agagtgatcg
     1681 gcgagggctg gaacagagcc atcggcctgc acgacccaac agcccatgcc gaaattatgg
     1741 ccctgagaca gggcggcctg gtcatgcaga actacagact gattgacgcc accctgtacg
     1801 tgacattcga gccttgcgtg atgtgcgccg gcgccatgat ccactctagg atcggccgcg
     1861 tggtgtttgg cgtgaggaac tcaaaaagag gcgccgcagg ctccctgatg aacgtgctga
     1921 actaccccgg catgaatcac cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg
     1981 ccgccctgct gtgcgatttc tatcggatgc ctagacaggt gttcaatgct cagaagaagg
     2041 cccagagctc catcaactcc ggaggatcta gcggaggctc ctctggctct gagacacctg
     2101 gcacaagcga gagcgcaaca cctgaaagca gcgggggcag cagcgggggg tcagacaaga
     2161 agtacagcat cggcctggcc atcggcacca actctgtggg ctgggccgtg atcaccgacg
     2221 agtacaaggt gcccagcaag aaattcaagg tgctgggcaa caccgaccgg cacagcatca
     2281 agaagaacct gatcggagcc ctgctgttcg acagcggcga aacagccgag gccacccggc
     2341 tgaagagaac cgccagaaga agatacacca gacggaagaa ccggatctgc tatctgcaag
     2401 agatcttcag caacgagatg gccaaggtgg acgacagctt cttccacaga ctggaagagt
     2461 ccttcctggt ggaagaggat aagaagcacg agcggcaccc catcttcggc aacatcgtgg
     2521 acgaggtggc ctaccacgag aagtacccca ccatctacca cctgagaaag aaactggtgg
     2581 acagcaccga caaggccgac ctgcggctga tctatctggc cctggcccac atgatcaagt
     2641 tccggggcca cttcctgatc gagggcgacc tgaaccccga caacagcgac gtggacaagc
     2701 tgttcatcca gctggtgcag acctacaacc agctgttcga ggaaaacccc atcaacgcca
     2761 gcggcgtgga cgccaaggcc atcctgtctg ccagactgag caagagcaga cggctggaaa
     2821 atctgatcgc ccagctgccc ggcgagaaga agaatggcct gttcggaaac ctgattgccc
     2881 tgagcctggg cctgaccccc aacttcaaga gcaacttcga cctggccgag gatgccaaac
     2941 tgcagctgag caaggacacc tacgacgacg acctggacaa cctgctggcc cagatcggcg
     3001 accagtacgc cgacctgttt ctggccgcca agaacctgtc cgacgccatc ctgctgagcg
     3061 acatcctgag agtgaacacc gagatcacca aggcccccct gagcgcctct atgatcaaga
     3121 gatacgacga gcaccaccag gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc
     3181 ctgagaagta caaagagatt ttcttcgacc agagcaagaa cggctacgcc ggctacattg
     3241 acggcggagc cagccaggaa gagttctaca agttcatcaa gcccatcctg gaaaagatgg
     3301 acggcaccga ggaactgctc gtgaagctga acagagagga cctgctgcgg aagcagcgga
     3361 ccttcgacaa cggcagcatc ccccaccaga tccacctggg agagctgcac gccattctgc
     3421 ggcggcagga agatttttac ccattcctga aggacaaccg ggaaaagatc gagaagatcc
     3481 tgaccttccg catcccctac tacgtgggcc ctctggccag gggaaacagc agattcgcct
     3541 ggatgaccag aaagagcgag gaaaccatca ccccctggaa cttcgaggaa gtggtggaca
     3601 agggcgcttc cgcccagagc ttcatcgagc ggatgaccaa cttcgataag aacctgccca
     3661 acgagaaggt gctgcccaag cacagcctgc tgtacgagta cttcaccgtg tataacgagc
     3721 tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc cgccttcctg agcggcgagc
     3781 agaaaaaggc catcgtggac ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc
     3841 tgaaagagga ctacttcaag aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg
     3901 aagatcggtt caacgcctcc ctgggcacat accacgatct gctgaaaatt atcaaggaca
     3961 aggacttcct ggacaatgag gaaaacgagg acattctgga agatatcgtg ctgaccctga
     4021 cactgtttga ggacagagag atgatcgagg aacggctgaa aacctatgcc cacctgttcg
     4081 acgacaaagt gatgaagcag ctgaagcggc ggagatacac cggctggggc aggctgagcc
     4141 ggaagctgat caacggcatc cgagacaagc agagtggaaa gacaatcctg gattttctta
     4201 agtccgatgg atttgccaac cggaacttca tgcagttgat ccatgatgac tctctcacct
     4261 ttaaggagga catccagaaa gcacaagttt gtctggctgg cgatactctc attaccctgg
     4321 ccgatggacg acgagtgcct attagagaac tggtgtcaca gcagaatttt tccgtgtggg
     4381 ctctgaatcc tcagacttac cgcctggaga gggctagagt gagtagagct ttctgtaccg
     4441 gcatcaaacc tgtgtaccgc ctcaccacta gactggggag atccattagg gccactgcca
     4501 accaccgatt tctcacacct cagggctgga aacgagtcga tgaactccag cctggagatt
     4561 acctggctct gcctaggaga atccctactg cctcctgatc tcgagctcga tgagtttgga
     4621 caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
     4681 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
     4741 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
     4801 aaatgtggta gcggccgcgg aatgtgtgtc agtaaaacct ctacaaatgt ggtagcggcc
     4861 gcaggtagat aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga
     4921 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     4981 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gccttaatta
     5041 acctaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     5101 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     5161 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     5221 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     5281 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     5341 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     5401 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     5461 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     5521 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     5581 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     5641 caaaatatta acgtttataa tttcaggtgg catctttcgg ggaaatgtgc gcggaacccc
     5701 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     5761 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     5821 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     5881 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     5941 caatagtggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     6001 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     6061 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     6121 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     6181 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     6241 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     6301 agccatacca aacgacgagc gtgacaccac gatgcctgta gtaatggtaa caacgttgcg
     6361 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     6421 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     6481 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     6541 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     6601 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     6661 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     6721 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     6781 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     6841 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     6901 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6961 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7021 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7081 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7141 ctgaacgggg ggttcgtgca cacagcccag ct
//