LOCUS       pAAV-CASI-inteinC-aa713 7245 bp ds-DNA     circular     14-MAR-2023
DEFINITION  .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name:
            addgene-plasmid-80932-sequence-155820.gbk Expresses Cas9C in
            mammalian cells; derived from pZac2.1 with CASI promoter..
FEATURES             Location/Qualifiers
     repeat_region   1..130
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     enhancer        206..509
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          314..335
                     /label="ABE-titer-F"
                     /note="sequence: ccattgacgtcaatgggtggag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          complement(465..486)
                     /label="ABE-Titer-R1"
                     /note="sequence: cgtagatgtactgccaagtagg"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     promoter        511..788
                     /label="chicken beta-actin promoter"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     regulatory      1262..1271
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     regulatory      1266..1275
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          1268..1292
                     /label="C-qPCR-titer-F"
                     /note="sequence: atggcggcggcgtgcccggaactgc"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1268..1423
                     /label="Rma intein C"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             1268..1433
                     /label="Translation 1268-1433"
                     /translation="MAAACPELRQLAQSDVYWDPIVSIEPDGVEEVFDLTVPGPHNFVANDIIAHNSGQ"
     CDS             1268..1524
                     /label="Translation 1268-1524"
                     /translation="MAAACPELRQLAQSDVYWDPIVSIEPDGVEEVFDLTVPGPHNFVANDIIAHNSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDE"
     misc_feature    1417..1433
                     /label="插入SpG C前重后"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1424..1433
                     /label="Cas9-C-term"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1434..1435
                     /label="扩片段2-前"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1434..1453
                     /label="扩片段前"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1448..1524
                     /label="insert aa713 SpG C"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             1448..3451
                     /label="Translation 1448-3451"
                     /translation="EHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESIRPKRNSDKLIARKKDWDPKKYGGFVSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASARFLQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPRAFKYFDTTIDRKVYRSTKEVLDATLIHQSITGLYETRIDLSQLGGDSGGSKRTADGSEFEPKKKRKV"
     misc_feature    1510..1524
                     /label="overlap"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    1525..3430
                     /label="NG C"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer          1965..1984
                     /label="NG C- seq 1"
                     /note="sequence: agagaaagttcgacaatctg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          2662..2681
                     /label="NG C- seq 2"
                     /note="sequence: ggaccctaagaagtacggcg"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer          complement(2784..2806)
                     /label="QPCR-NG-C-R1"
                     /note="sequence: gaagctgcttctttccatgatgg"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3431..3451
                     /label="NLS"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3460..3486
                     /label="插入SpG C后重合"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_site      3466..3521
                     /label="-beta-globin poly(A) signal"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(3520..3539)
                     /label="rbglobpA-R"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3587..3605
                     /label="插入U6前"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(3626..3701)
                     /label="gRNA scaffold"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          complement(3702..3721)
                     /label="sgRNA"
                     /note="sequence: GCTTGCACATGGCCACTTCC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(3730..3970)
                     /label="U6 promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    3981..3995
                     /label="插入U6后"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     repeat_region   4305..4434
                     /label="AAV2 ITR"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(4453..4469)
                     /label="M13 fwd"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer_bind     complement(4453..4470)
                     /label="M13 Forward"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(4462..4484)
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     rep_origin      4611..5066
                     /label="f1 ori"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(4698..4717)
                     /label="F1ori-R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     4908..4929
                     /label="F1ori-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     promoter        5093..5197
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             5198..6058
                     /label="AmpR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(5416..5435)
                     /label="Amp-R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     rep_origin      6229..6817
                     /label="ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     6718..6737
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     primer_bind     6971..6988
                     /label="L4440"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     protein_bind    7105..7126
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     promoter        7141..7171
                     /label="lac promoter"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     protein_bind    7179..7195
                     /label="lac operator"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     7184..7206
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     7203..7219
                     /label="M13 rev"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     7203..7219
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      241 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      301 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      361 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      421 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      481 tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc
      541 tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt
      601 gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg
      661 aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc
      721 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc
      781 ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc
      841 gccgcccgcc ccggctctga ctgaccgcgt tactaaaaca ggtaagtccg gcctccgcgc
      901 cgggttttgg cgcctcccgc gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac
      961 gaagggcgca gcgagcgtcc tgatccttcc gcccggacgc tcaggacagc ggcccgctgc
     1021 tcataagact cggccttaga accccagtat cagcagaagg acattttagg acgggacttg
     1081 ggtgactcta gggcactggt tttctttcca gagagcggaa caggcgagga aaagtagtcc
     1141 cttctcggcg attctgcgga gggatctccg tggggcggtg aacgccgatg atgcctctac
     1201 taaccatgtt catgttttct ttttttttct acaggtcctg ggtgacgaac aggctagcgc
     1261 cgccaccatg gcggcggcgt gcccggaact gcgtcagctg gcgcagagcg atgtgtattg
     1321 ggatccgatt gtgagcattg aaccggatgg cgtggaagaa gtgtttgatc tgaccgtgcc
     1381 gggcccgcat aactttgtgg cgaacgatat tattgcgcat aactctggcc agggcgatag
     1441 cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca
     1501 gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat
     1561 cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga
     1621 gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca
     1681 ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg
     1741 gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga
     1801 ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag
     1861 aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat
     1921 gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa
     1981 tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag
     2041 acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat
     2101 gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa
     2161 gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa
     2221 caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa
     2281 aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg
     2341 gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta
     2401 cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa
     2461 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga
     2521 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga
     2581 ggtgcagaca ggcggcttca gcaaagagtc tatcaggccc aagaggaaca gcgataagct
     2641 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgtca gccccaccgt
     2701 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag
     2761 tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat
     2821 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
     2881 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccagatt
     2941 cctgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
     3001 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
     3061 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
     3121 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
     3181 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
     3241 agcccctagg gccttcaagt actttgacac caccatcgac cggaaggtgt acaggagcac
     3301 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
     3361 gatcgacctg tctcagctgg gaggtgactc tggcggctca aaaagaaccg ccgacggcag
     3421 cgaattcgag cccaagaaga agaggaaagt cTAAACCGGt tggctaataa aggaaattta
     3481 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     3541 gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc
     3601 atatgaggcc ctcacgcgta aaaaagcacc gactcggtgc cactttttca agttgataac
     3661 ggactagcct tattttaact tgctatttct agctctaaaa cGGAAGTGGC CATGTGCAAG
     3721 Cggtgtttcg tcctttccac aagatatata aagccaagaa atcgaaatac tttcaagtta
     3781 cggtaagcat atgatagtcc attttaaaac ataattttaa aactgcaaac tacccaagaa
     3841 attattactt tctacgtcac gtattttgta ctaatatctt tgtgtttaca gtcaaattaa
     3901 ttccaattat ctctctaaca gccttgtatc gtatatgcaa atatgaagga atcatgggaa
     3961 ataggccctc ggtaccggcg atgctggctg ccatgaacaa aggttggcta taaagaggtc
     4021 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     4081 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     4141 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     4201 ctgtccctct tctcttatgg agatcggatc cgaattcccg ataaggatct tcctagagca
     4261 tggctacgta gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat
     4321 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt
     4381 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagccttaa
     4441 ttaacctaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4501 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     4561 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     4621 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4681 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4741 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4801 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4861 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4921 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     4981 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5041 taacaaaata ttaacgttta taatttcagg tggcatcttt cggggaaatg tgcgcggaac
     5101 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5161 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     5221 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     5281 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     5341 tctcaatagt ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     5401 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     5461 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     5521 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     5581 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     5641 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     5701 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagtaatgg taacaacgtt
     5761 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     5821 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     5881 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     5941 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6001 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6061 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6121 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6181 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     6241 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     6301 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     6361 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     6421 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     6481 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     6541 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     6601 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     6661 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     6721 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     6781 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     6841 acggttcctg gccttttgct gcggttttgc tcacatgttc tttcctgcgt tatcccctga
     6901 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     6961 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7021 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     7081 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     7141 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     7201 cacaggaaac agctatgacc atgattacgc cagatttaat taagg
//