LOCUS       pCD-Ugi21               3476 bp    DNA     circular UNA 02-AUG-2023
DEFINITION  pGGC000 Greengate entry vector containing Utricularia gibba
            insulator 21 (Ugi21) sequence (ID9063).
ACCESSION   urn.local...11-gl0oh3s
VERSION     urn.local...11-gl0oh3s
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    96..200
                     /gene="bla"
                     /label="ampR promoter"
                     /note="Geneious type: Promoter (prokaryote)"
     CDS             201..1061
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="ampR"
     misc_feature    201..1061
                     /created_by="eudald"
                     /label="ampR"
                     /note="Geneious type: Selectable Marker"
     rep_origin      1232..1820
                     /direction="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="pBR322 origin"
     misc_feature    2144..2174
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
                     /note="Geneious type: Promoter (prokaryote)"
     misc_feature    2212..2230
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
                     /note="Geneious type: Promoter (prokaryote)"
     misc_feature    2228..3065
                     /Source="referenceToDocument: urn:local:.:35-gl0p3vt"
                     /modified_by="eudald"
                     /label="CD_Ugi21 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     GreenGate       2228..2253
                     /created_by="eudald"
                     /modified_by="eudald"
                     /Transferred_From="Homology arm GreenGate (C)"
                     /Transferred_Similarity="100.00%"
                     /label="GreenGate Homology arm (C)"
     ligation        2228..2251
                     /label="Ligation"
     misc_feature    2241..2246
                     /Recognition_pattern="GGTCTC(1/5)"
                     /Methylation="Dcm:variable, CpG:variable, EcoBI:cut"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     Insulator       2254..3041
                     /created_by="eudald"
                     /modified_by="eudald"
                     /label="Ugi21"
     misc_feature    3042..2251
                     /Source="referenceToDocument: urn:local:.:36-gl0p3vt"
                     /label="pGGC000 - digested BsaI (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     GreenGate       3042..3065
                     /created_by="eudald"
                     /modified_by="eudald"
                     /Transferred_From="Homology arm GreenGate (D)"
                     /Transferred_Similarity="100.00%"
                     /label="GreenGate Homology arm (D)"
     ligation        3042..3065
                     /label="Ligation"
     misc_feature    complement(3047..3052)
                     /Recognition_pattern="GGTCTC(1/5)"
                     /Methylation="Dcm:variable, CpG:variable, EcoBI:cut"
                     /annotation_group="GGTCTC"
                     /label="BsaI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(3063..3081)
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
                     /note="Geneious type: Promoter (prokaryote)"
ORIGIN      
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtggctct cgaggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacgagc tatttaggtg
     2221 acactataga agtgaagctt ggtctcaggc tcccggatga atttaaatat aaatggcgtc
     2281 tattataatt ggtctacacg agccgagcct gggggtggct ttctccagat attttacccg
     2341 ttccacagct tcattctgag attactttat tctctttcgg ttgtgtaaga tcctacctta
     2401 tacaacggaa ggatataaaa gcaattactt tattgttatg aataaccttt ttattaattt
     2461 aagagtgtgc ggttggttat atatcaggta ttgggaagta tggaacttgt tggttttcga
     2521 tgggttcagc tatatctgtt tgtgggtgcg ctgaaattga ttttggcatg ttagctctat
     2581 ggttttcact cttttcttgc ttttctatgg catgtgttca gttactatgc ttattagtac
     2641 aatgctccac aattctggac tgtggatgtt ttaacttttt tggaatcatc cattctatca
     2701 catttgacac ttgaaattgc aggtgtctgt gatcagcact gcatgctgga ttctatctta
     2761 gaaccgttcg actgttcatg tctagggttg gttccatttt cagccacctg ggaaggtctg
     2821 tgcggtatat atatgagaat agtgcgatgg atgcttccag caaagccttc atcctccagc
     2881 ggtgatcagg atggacatag agaatgatca accatcgtct cccttttggg tggaaatgcg
     2941 tgtacagaca gacatgacac acttttgcgc tggggcgcct gggagcgttt gctgcattta
     3001 ggcttcatcc ttccgtacgg tgaactttgc aaatgcgcaa ctcagtgaga ccgaattctc
     3061 gccctatagt gagtcgtatt acctggccgt cgttttacaa cgtcgtgact gggaaaaccc
     3121 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     3181 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     3241 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     3301 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     3361 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     3421 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcga
//
