LOCUS       CTerm_Halo-3xFLA        8410 bp DNA     circular SYN 21-JAN-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 8410)
  AUTHORS   IQ
  TITLE     Direct Submission
  JOURNAL   Exported Aug 2, 2023 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8410
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     rep_origin      102..557
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        584..688
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             689..1549
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  689 ..  757 / #ccffcc / signal sequence
                        2:  758 .. 1549 / #ccffcc
                     Cleavage site after base 758"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1720..2308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     mobile_element  2611..2835
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
                     /note="color: #ffe4c4"
     CDS             complement(2902..3435)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /note="color: #ccffcc"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3624..3652)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
                     /note="This reverse directional feature has 3 segments:
                        1: 3624 .. 3629 / #ffffff / -10
                        2: 3630 .. 3646 / #ffffff
                        3: 3647 .. 3652 / #ffffff / -35"
     polyA_signal    4161..4209
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
                     /note="color: #a6acb3"
     promoter        complement(4345..4454)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
                     /note="color: #ffffff; direction: LEFT"
     promoter        4478..4569
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    4803..4827
                     /label=pfastbac overlap
                     /note="color: #ff6600"
     misc_feature    5267..5276
                     /label=RIF1 END
                     /note="color: #a6acb3"
     misc_feature    5277..5333
                     /label=linker
                     /note="color: #3366ff"
     misc_feature    5277..5301
                     /label=Tag Overlap
                     /note="color: #ff6600"
     misc_feature    5277..5296
                     /label=LHA
                     /note="color: #a6acb3"
     misc_feature    5277..5296
                     /label=LHA
                     /note="color: #ff0000"
     primer_bind     5277..5296
                     /label=oJS812
                     /note="color: black; sequence: ggagctctcgagccaaccac; added:
                     2019-10-28"
     CDS             5298..5318
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /note="color: #cc99b2
                     Cleavage site after base 5315"
                     /translation="EDLYFQS"
     CDS             5334..6218
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /note="color: #cc99b2"
                     /translation="EIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSSYV
                     WRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVIHD
                     WGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLIIDQ
                     NVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALVEE
                     YMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDLIG
                     SEIARWLSTLEISG"
     CDS             6219..6284
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 6284"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    6317..6541
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     promoter        6563..6892
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      6743..6878
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     complement(6876..6921)
                     /label=oDB147
                     /note="color: black; sequence: 
                     gtgggcttgtactcggtcatggtaagctttttgcaaaagcctaggc"
     CDS             6902..7501
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6902..6921
                     /label=oDB148
                     /note="color: black; sequence: atgaccgagtacaagcccac"
     misc_feature    7467..7484
                     /label=FW Primer in Puro
                     /note="color: #a6acb3"
     polyA_signal    7502..7550
                     /note="synthetic polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    7526..7550
                     /label=Tag Overlap
                     /note="color: #ff6600"
     primer_bind     complement(7526..7550)
                     /label=oDB98
                     /note="color: black; sequence: cacacaaaaaaccaacacacagatg; 
                     added: 2019-10-28"
     misc_feature    7531..7999
                     /label=RHA
                     /note="This feature has 2 segments:
                        1: 7531 .. 7550 / #0000ff
                        2: 7551 .. 7999 / #a6acb3"
     misc_feature    7533..7550
                     /label=RHA
                     /note="color: #a6acb3"
     polyA_signal    8000..8134
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    8000..8024
                     /label=pfastbac overlap
                     /note="color: #ff6600"
     mobile_element  8163..8328
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
                     /note="color: #ffe4c4"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
      121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
      181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
      241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
      301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
      361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
      421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
      481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
      541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
      601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
      661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
      721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
      781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
      841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
      901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
      961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg
     2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa
     2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga
     2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact
     2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg
     2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc
     2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac
     2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc
     3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag
     3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat
     3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc
     3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta
     3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag
     3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat
     3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg
     3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga
     3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg
     3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc
     3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg
     3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg
     3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc
     3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag
     3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct
     3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac
     4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga
     4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg
     4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt
     4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc
     4261 tcccccatct cccggtaccg catgctatgc atcagctgct agcaccatgg ctcgagatcc
     4321 cgggtgatca agtcttcgtc gagtgattgt aaataaaatg taatttacag tatagtattt
     4381 taattaatat acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg
     4441 ttgaattaaa ggtccgtata ctccggaata ttaatagatc atggagataa ttaaaatgat
     4501 aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa
     4561 cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc ccggtccgaa
     4621 gcgcgcggaa ttcaaaggcc tacgtcgacg agctcactag tcgcggccgc tttcgaatct
     4681 agagcctgca gtctcgacaa gcttgtcgag aagtactaga ggatcataat cagccatacc
     4741 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4801 cataaaatga atgcaattgt tgttgttgag ctgagattac gccactgcac tccagcctgg
     4861 gcaatagaat gaggctccat ctcataataa taataagcag cagcggcagc tctggtagat
     4921 ttttttgtgt gcttgtcctc atgaagactt aagcctgctc tctcaatttg aaacctaggc
     4981 acttggacta acttaaaaga tcatgtaaaa aatttaattt ttgtctggta tagatttaat
     5041 tgtggttttt tttttctctt ttagatataa ttgatcctgt tgctttagaa attccattat
     5101 ccaaaaacct tctggcacag attagtgctc ttgctcttca gctggattca gaagatcttc
     5161 ataattattc aggaagccaa ctatttgaaa tgcacgagaa actaagttgt atggcaaact
     5221 ctgtaataaa aaatctacag tcacgttgga gatcaccatc ccatgaaaat tctattggag
     5281 ctctcgagcc aaccactgag gatctgtact ttcagagcga taacgatgga tccgaaatcg
     5341 gtactggctt tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg
     5401 tcgatgttgg tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct
     5461 cctacgtgtg gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc attgctccag
     5521 acctgatcgg tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg
     5581 tccgcttcat ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc
     5641 acgactgggg ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag
     5701 gtattgcatt tatggagttc atccgcccta tcccgacctg ggacgaatgg ccagaatttg
     5761 cccgcgagac cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc
     5821 agaacgtttt tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg
     5881 agatggacca ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct
     5941 tcccaaacga gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg gtcgaagaat
     6001 acatggactg gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg
     6061 ttctgatccc accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg
     6121 tggacatcgg cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg
     6181 agatcgcgcg ctggctgtct actctggaga tttccggtga ctacaaagac catgacggtg
     6241 attataaaga tcatgatatc gattacaagg atgacgatga caagtaactc gagtctagac
     6301 gtttaaaccc tgcaggctgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     6361 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     6421 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     6481 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     6541 ggtcgacagt actaagcttt acgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6601 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     6661 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6721 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6781 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     6841 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac
     6901 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6961 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     7021 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     7081 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     7141 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     7201 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     7261 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     7321 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     7381 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     7441 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     7501 aaataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg tattttcaga
     7561 gaaaattgaa ggttttttta aacatcactg gatttcttga ttgaggaaac aagttctgaa
     7621 ataatagcac aatttcaaag aagagactct ttgcaaagtt gataacattt caaaccctga
     7681 aggacagtga cttattatgt aagttcaatt ttgtaagttc attatgtaag atcctttttt
     7741 ttttcataat atgtattctt ggctgctatg cgtggttttt caggaaattt aattatctta
     7801 ctgagatgtg aaagcaaaac tagtaacaga acttacattt tatttcatgc tttcttaaac
     7861 ccgtgcatat tctggtgaaa catgtaaaat actttaagta aaattgaaca tttttatttg
     7921 aatttttgct gaactgataa aggtgtttat atttttgttt gttggtttgt ttaattcatg
     7981 tttgttggga ctgaggttta acttgtttat tgcagcttat aatggttaca aataaagcaa
     8041 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8101 caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctaggagatc
     8161 cgaaccagat aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag
     8221 cttacgacgc tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa
     8281 aactccattt ccacccctcc cagttcccaa ctattttgtc cgcccacagc ggggcatttt
     8341 tcttcctgtt atgtttttaa tcaaacatcc tgccaactcc atgtgacaaa ccgtcatctt
     8401 cggctacttt
//
