LOCUS       Exported                8229 bp DNA     circular SYN 11-AUG-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   MSU-IQ
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2023 from SnapGene 6.0.3
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        484..588
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             589..1449
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  589 ..  657 / #ccffcc / signal sequence
                        2:  658 .. 1449 / #ccffcc
                     Cleavage site after base 657"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1620..2208
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
                     /note="color: #ffe4c4"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /note="color: #ccffcc"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(3524..3552)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
                     /note="This reverse directional feature has 3 segments:
                        1: 3524 .. 3529 / #ffffff / -10
                        2: 3530 .. 3546 / #ffffff
                        3: 3547 .. 3552 / #ffffff / -35"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    4241..4265
                     /label=pFASTBAC
                     /note="color: #a6acb3"
     misc_feature    4266..4285
                     /label=LHA start
                     /note="color: #a6acb3"
     misc_feature    4905..4964
                     /label=Forward Homology
                     /note="color: #ff00ff"
     misc_feature    4965..7057
                     /label=DNAPKcs START
                     /note="color: #a6acb3"
     primer_bind     4965..4989
                     /label=Primer 1
                     /note="color: black; sequence: atgGACTACAAAGACCATGACGGTG"
     misc_feature    4968..5033
                     /label=3XFLAG
                     /note="color: #da8c81"
     misc_feature    5034..5067
                     /label=LoxP+2
                     /note="color: #ffec19"
     misc_feature    5068..5073
                     /label=HindIII
                     /note="color: #00ff00"
     misc_feature    complement(5074..5122)
                     /label=Synthetic poly(A)
                     /note="color: #ffafb8; direction: LEFT"
     misc_feature    complement(5123..5722)
                     /label=Puro
                     /note="color: #0080ff; direction: LEFT"
     misc_feature    complement(5777..6045)
                     /label=SV40 promoter
                     /note="color: #ff80ff; direction: LEFT"
     misc_feature    complement(6046..6051)
                     /label=BamHI
                     /note="color: #00ff00; direction: LEFT"
     misc_feature    6052..6085
                     /label=LoxP+2(2)
                     /note="color: #ffec19"
     misc_feature    6086..6087
                     /label=LoxP+2(1)
                     /note="color: #ffec19"
     misc_feature    6086..6087
                     /label=Extra Bases
                     /note="color: #f61cff"
     misc_feature    6088..6093
                     /label=KpnI
                     /note="color: #00ff00"
     misc_feature    6094..6119
                     /label=Halo START
                     /note="color: #ff1ef9"
     misc_feature    7016..7035
                     /label=HALO END
                     /note="color: #2eff3c"
     primer_bind     complement(7023..7041)
                     /label=Primer 2
                     /note="color: black; sequence: ggtaccGGAAGCGATCGCG"
     misc_feature    7036..7041
                     /label=KpnI(1)
                     /note="color: #05ff00"
     misc_feature    7042..7102
                     /label=Reverse Homology
                     /note="color: #ff0000"
     misc_feature    7699..7718
                     /label=RHA end
                     /note="color: #a6acb3"
     polyA_signal    7719..7853
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     mobile_element  7882..8047
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
                     /note="color: #ffe4c4"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
     2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatcccgg tccgaagcgc gcggaattca aaggcctacg tcgacgagct
     4081 cactagtcgc ggccgctttc gaatctagag cctgcagtct cgaggcatgc ggtaccaagc
     4141 ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag gttttacttg
     4201 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg
     4261 ttgttggggg ggacagtgcc gcttctttca gcgcccgtgc gtaaaccaga agtaggcctc
     4321 gctcggcccc ccttggtcca gatggtctga ccccctgcgc ggcagcgttt tcgcgggaaa
     4381 actgggtcct acagcaccag ctctcggggt gtgttcctct agcgttggct agggagcgtg
     4441 tgcttcttta tcccttaggc gtttttggtt aataaggaca ttacagatgc atttctcact
     4501 taaagatttt ttttattaaa aaaaattaag gacgggaggt ggtggggatg gtctcattat
     4561 gttgcccaga ctggtctcga actcctgcga tccccccgcc tcagcttcct aaagtgttgg
     4621 gactgcaggc gggcatcacc gcccccgacc gcgtttgtcg ttttttatcg aggaacaaac
     4681 ttggaactct tgacctaggc ccctcgcttg ttttatctgc ctctggtatt tatttagcca
     4741 agtccaacac caagtagcca cccaaactac ctccgcaggt cagacgtttt tccttaggtt
     4801 tccatgttga ttcgggccaa agaggcgcgc ttactggcca ggccttcccg caggggtccc
     4861 cgggaaagtt cctgccgccg cgccccgcag ccccgcctcc gcgcgtaggg gcatttccgg
     4921 gtccgggccg agcgggcgca cgcgcgggag cgggactcgg cggcatggac tacaaagacc
     4981 atgacggtga ttataaagat catgatatcg attacaagga tgacgatgac aagataactt
     5041 cgtataatgt atgctatacg aagttataag cttcacacaa aaaaccaaca cacagatgta
     5101 atgaaaataa agatatttta tttcaggcac cgggcttgcg ggtcatgcac caggtgcgcg
     5161 gtccttcggg cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga
     5221 ggttgcgggg cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca
     5281 ctccggggag cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga
     5341 cggtggccag gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct
     5401 gttgctgcgc ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga
     5461 acaccgcccc cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt
     5521 ccgcgaccca caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct
     5581 cggtgacccg ctcgatgtgg cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg
     5641 cgaacgcggc ggcgagggtg cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca
     5701 ccgtgggctt gtactcggtc atgagctttt tgcaaaagcc taggcctcca aaaaagcctc
     5761 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     5821 aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     5881 gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt tgcatacttc
     5941 tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg agatgcatgc
     6001 tttgcatact tctgcctgct ggggagcctg gggactttcc acaccggatc cataacttcg
     6061 tataatgtat gctatacgaa gttatccggt accatggcag aaatcggtac tggctttcca
     6121 ttcgaccccc attatgtgga agtcctgggc gagcgcatgc actacgtcga tgttggtccg
     6181 cgcgatggca cccctgtgct gttcctgcac ggtaacccga cctcctccta cgtgtggcgc
     6241 aacatcatcc cgcatgttgc accgacccat cgctgcattg ctccagacct gatcggtatg
     6301 ggcaaatccg acaaaccaga cctgggttat ttcttcgacg accacgtccg cttcatggat
     6361 gccttcatcg aagccctggg tctggaagag gtcgtcctgg tcattcacga ctggggctcc
     6421 gctctgggtt tccactgggc caagcgcaat ccagagcgcg tcaaaggtat tgcatttatg
     6481 gagttcatcc gccctatccc gacctgggac gaatggccag aatttgcccg cgagaccttc
     6541 caggccttcc gcaccaccga cgtcggccgc aagctgatca tcgatcagaa cgtttttatc
     6601 gagggtacgc tgccgatggg tgtcgtccgc ccgctgactg aagtcgagat ggaccattac
     6661 cgcgagccgt tcctgaatcc tgttgaccgc gagccactgt ggcgcttccc aaacgagctg
     6721 ccaatcgccg gtgagccagc gaacatcgtc gcgctggtcg aagaatacat ggactggctg
     6781 caccagtccc ctgtcccgaa gctgctgttc tggggcaccc caggcgttct gatcccaccg
     6841 gccgaagccg ctcgcctggc caaaagcctg cctaactgca aggctgtgga catcggcccg
     6901 ggtctgaatc tgctgcaaga agacaacccg gacctgatcg gcagcgagat cgcgcgctgg
     6961 ctgtcgacgc tcgagatttc cggcgagcca accactgagg atctgtactt tcagagcgat
     7021 aacgcgatcg cttccggtac cgcgggctcc ggagccggtg tgcgttgctc cctgctgcgg
     7081 ctgcaggaga ccttgtccgc tgcggaccgc tgcggtgctg ccctggccgg tcatcaactg
     7141 atccgcggcc tggggcagga atgcgtcctg agcagcagcc ccgcggtgct gggtgggtac
     7201 cggcccgagc tgggccgcgg gtgggtcctg gcttcccgag tcgttctgga gccgggcagc
     7261 ccggcgcttc tctgtgttta gatgacggta ttttgaagta cagcaattag attcttgggg
     7321 gaatttctgt gtatgtgtat aaagggattt gtcatactgc tttttacccc ctagtattag
     7381 aggaaagata ttttaaagcc aaagttggtg aaaacgaccc ctaatatggc aatcattgct
     7441 atgcagagtg gggggcaggg caagagaaat aatttttttt ttttttgaga cggagtctcg
     7501 ctctgtcgcc caggctggag tgaagtggcg cgatctcggc tcactgcaac ctccgcctcc
     7561 cgggttcaag ctattctcct gcctcagccc cccgagtagc tgggattaca ggcgcgcgcc
     7621 accacgcccg gctaattttt ttgtattttt agtagagatg gggtttcacc atgttggtca
     7681 ggctggtctc gaactcctga cctcgtgatc cccccgccaa cttgtttatt gcagcttata
     7741 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7801 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac
     7861 tgcttgagcc taggagatcc gaaccagata agtgaaatct agttccaaac tattttgtca
     7921 tttttaattt tcgtattagc ttacgacgct acacccagtt cccatctatt ttgtcactct
     7981 tccctaaata atccttaaaa actccatttc cacccctccc agttcccaac tattttgtcc
     8041 gcccacagcg gggcattttt cttcctgtta tgtttttaat caaacatcct gccaactcca
     8101 tgtgacaaac cgtcatcttc ggctactttt tctctgtcac agaatgaaaa tttttctgtc
     8161 atctcttcgt tattaatgtt tgtaattgac tgaatatcaa cgcttatttg cagcctgaat
     8221 ggcgaatgg
//
