LOCUS       pBS-EF1afull-H2B       11906 bp ds-DNA     circular     04-AUG-2023
DEFINITION  .
ACCESSION   urn.local...5d-gl3i2n9
KEYWORDS    "marker:AmpR, BleoR" "ref:pmid\:25075903".
SOURCE      
  ORGANISM  .
COMMENT     Expresses human codon-optimized S. pyogenes Cas9 protein and       
            blasticidin resistance from EFS promoter. 3rd generation lentiviral
            backbone.. <small>[1] Improved vectors and genome-wide libraries   
            for CRISPR screening. Nat Methods. 2014 Aug
            11(8):783-4. doi:       10.1038/nmeth.3047. Sanjana NE, Shalem O,
            Zhang F                   (pmid:25075903)</small>.
FEATURES             Location/Qualifiers
     misc_feature    119..299
                     /note="Geneious type: LTR"
                     /standard_name="5' LTR (truncated)"
     misc_feature    331..555
                     /note="Geneious type: polyA_signal"
                     /standard_name="bGH poly(A) signal"
     rep_origin      601..1029
     rep_origin      1203..1337
     regulatory      1399..1446
                     /note="Geneious type: promoter"
                     /standard_name="EM7 promoter"
     CDS             1465..1839
                     /standard_name="BleoR"
     misc_feature    1969..2090
                     /note="Geneious type: polyA_signal"
                     /standard_name="SV40 poly(A) signal"
     primer_bind     complement(2139..2155)
                     /standard_name="M13 rev"
     protein_bind    2163..2179
                     /standard_name="lac operator"
     regulatory      complement(2187..2193)
                     /note="Geneious type: promoter"
                     /standard_name="lac promoter"
     regulatory      complement(2194..2211)
                     /note="Geneious type: promoter"
                     /standard_name="lac promoter"
     regulatory      complement(2212..2217)
                     /note="Geneious type: promoter"
                     /standard_name="lac promoter"
     protein_bind    2232..2253
                     /standard_name="CAP binding site"
     rep_origin      complement(2541..3129)
     CDS             complement(3300..4091)
                     /standard_name="AmpR"
     CDS             complement(4092..4160)
                     /standard_name="AmpR"
     regulatory      complement(4161..4265)
                     /note="Geneious type: promoter"
                     /standard_name="AmpR promoter"
     regulatory      4531..4910
                     /note="Geneious type: enhancer"
                     /standard_name="CMV enhancer"
     regulatory      4912..5110
                     /note="Geneious type: promoter"
                     /standard_name="CMV promoter"
     misc_feature    5128..5308
                     /note="Geneious type: LTR"
                     /standard_name="5' LTR (truncated)"
     misc_feature    5355..5480
                     /standard_name="HIV-1 Ψ"
     misc_feature    5792..5836
                     /note="Geneious type: primer"
                     /note="sequence:
                     caaaagtaagaccaccgcacagcaagcggccgctgatcttcagac"
                     /standard_name="RREGibFwd"
     misc_feature    5973..6206
                     /standard_name="RRE"
     misc_feature    6733..6850
                     /standard_name="cPPT/CTS"
     regulatory      6900..7111
                     /note="Geneious type: promoter"
                     /standard_name="EF-1-alpha core promoter"
     intron          7112..8050
                     /standard_name="EF-1-alpha intron A"
     misc_feature    complement(7207..7226)
                     /note="Geneious type: primer"
                     /note="sequence: cccgaagctcgggatcaaga"
                     /standard_name="SeqEF1aRev"
     misc_feature    7552..7571
                     /note="Geneious type: primer"
                     /note="sequence: gagaatcggacgggggtagt"
                     /standard_name="SeqEF1aFwd"
     misc_feature    complement(7893..7912)
                     /note="Geneious type: primer"
                     /note="sequence: gtggggaaactccatcgcat"
                     /standard_name="SeqEF1aRev2"
     primer_bind     8007..8027
                     /standard_name="EF1a-F"
     CDS             8068..8445
                     /standard_name="H2B"
     CDS             8068..10887
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYK
                     VLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLL
                     LPGELAKHAVSEGTKAITKYTSAKPRMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEF
                     EIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKL
                     SFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGW
                     EASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDI
                     TSHNEDYTIVEQYERAEGRHSTGGMDELYKGSGTRATNFSLLKQAGDVEENPGPSMED
                     AKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVDITYAEYFEMSV
                     RLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMG
                     ISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFN
                     EYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDT
                     AILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFS
                     FFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILI
                     TPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATN
                     ALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDA
                     GVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKG
                     LTGKLDARKIREILIKAKKGGKIAV*"
                     /standard_name="Translation 8068-10887"
     CDS             8452..9159
                     /standard_name="mCherry"
     primer_bind     complement(8597..8615)
                     /standard_name="mCherry-R"
     primer_bind     8865..8884
                     /standard_name="mCherry-F"
     misc_feature    9169
                     /standard_name="MCS Cargo"
     misc_feature    9175..9231
                     /standard_name="P2A"
     misc_feature    9233..9234
                     /standard_name="F2A"
     CDS             9235..10887
                     /standard_name="CDS (luciferase luc2)"
     misc_feature    10912..11500
                     /standard_name="WPRE"
     misc_feature    10989..11005
                     /standard_name="qWPRE_probe"
     CDS             complement(11383..11394)
                     /standard_name="Factor Xa site"
     primer_bind     complement(11503..11519)
                     /standard_name="KS primer"
ORIGIN      
        1 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
       61 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
      121 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
      181 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
      241 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
      301 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
      361 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
      421 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
      481 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
      541 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
      601 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      661 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      721 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      781 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      841 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      901 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      961 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     1021 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     1081 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     1141 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     1201 agtcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     1261 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     1321 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     1381 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     1441 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     1501 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     1561 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     1621 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     1681 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     1741 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     1801 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     1861 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     1921 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     1981 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     2041 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     2101 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     2161 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     2221 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     2281 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     2341 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     2401 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     2461 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2521 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2581 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2641 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2701 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2761 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2821 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2881 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2941 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     3001 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     3061 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     3121 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     3181 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     3241 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     3301 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     3361 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     3421 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3481 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3541 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3601 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3661 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3721 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3781 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3841 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3901 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3961 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     4021 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     4081 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     4141 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     4201 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     4261 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     4321 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     4381 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     4441 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     4501 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     4561 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     4621 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     4681 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     4741 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     4801 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     4861 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     4921 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     4981 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     5041 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     5101 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     5161 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5221 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5281 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     5341 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     5401 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     5461 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     5521 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     5581 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     5641 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     5701 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     5761 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     5821 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     5881 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     5941 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     6001 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     6061 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     6121 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     6181 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     6241 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     6301 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     6361 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     6421 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     6481 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     6541 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     6601 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     6661 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
     6721 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     6781 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     6841 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa ttagctagcg
     6901 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgatc
     6961 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     7021 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     7081 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     7141 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     7201 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     7261 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     7321 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     7381 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     7441 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     7501 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     7561 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     7621 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     7681 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     7741 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     7801 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     7861 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     7921 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     7981 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     8041 tccatttcag tctagagcgc tgccaccatg ccagagccag cgaagtctgc tcccgccccg
     8101 aaaaagggct ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag
     8161 cgcagccgca aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct
     8221 gacaccggca tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc
     8281 gagcgcatcg caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc
     8341 tccagggaga tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc
     8401 gtgtccgagg gtactaaggc catcaccaag tacaccagcg ctaagcctag gatggtgagc
     8461 aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg
     8521 gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
     8581 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg
     8641 gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac
     8701 atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac
     8761 ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc
     8821 atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag
     8881 aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag
     8941 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag
     9001 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag
     9061 ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag
     9121 ggccgccact ccaccggcgg catggacgag ctgtacaagg gatccggcac gcgtgcaaca
     9181 aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc gtccatggaa
     9241 gatgccaaaa acattaagaa gggcccagcg ccattctacc cactcgaaga cgggaccgcc
     9301 ggcgagcagc tgcacaaagc catgaagcgc tacgccctgg tgcccggcac catcgccttt
     9361 accgacgcac atatcgaggt ggacattacc tacgccgagt acttcgagat gagcgttcgg
     9421 ctggcagaag ctatgaagcg ctatgggctg aatacaaacc atcggatcgt ggtgtgcagc
     9481 gagaatagct tgcagttctt catgcccgtg ttgggtgccc tgttcatcgg tgtggctgtg
     9541 gccccagcta acgacatcta caacgagcgc gagctgctga acagcatggg catcagccag
     9601 cccaccgtcg tattcgtgag caagaaaggg ctgcaaaaga tcctcaacgt gcaaaagaag
     9661 ctaccgatca tacaaaagat catcatcatg gatagcaaga ccgactacca gggcttccaa
     9721 agcatgtaca ccttcgtgac ttcccatttg ccacccggct tcaacgagta cgacttcgtg
     9781 cccgagagct tcgaccggga caaaaccatc gccctgatca tgaacagtag tggcagtacc
     9841 ggattgccca agggcgtagc cctaccgcac cgcaccgctt gtgtccgatt cagtcatgcc
     9901 cgcgacccca tcttcggcaa ccagatcatc cccgacaccg ctatcctcag cgtggtgcca
     9961 tttcaccacg gcttcggcat gttcaccacg ctgggctact tgatctgcgg ctttcgggtc
    10021 gtgctcatgt accgcttcga ggaggagcta ttcttgcgca gcttgcaaga ctataagatt
    10081 caatctgccc tgctggtgcc cacactattt agcttcttcg ctaagagcac tctcatcgac
    10141 aagtacgacc taagcaactt gcacgagatc gccagcggcg gggcgccgct cagcaaggag
    10201 gtaggtgagg ccgtggccaa acgcttccac ctaccaggca tccgccaggg ctacggcctg
    10261 acagaaacaa ccagcgccat tctgatcacc cccgaagggg acgacaagcc tggcgcagta
    10321 ggcaaggtgg tgcccttctt cgaggctaag gtggtggact tggacaccgg taagacactg
    10381 ggtgtgaacc agcgcggcga gctgtgcgtc cgtggcccca tgatcatgag cggctacgtt
    10441 aacaaccccg aggctacaaa cgctctcatc gacaaggacg gctggctgca cagcggcgac
    10501 atcgcctact gggacgagga cgagcacttc ttcatcgtgg accggctgaa gagcctgatc
    10561 aaatacaagg gctaccaggt agccccagcc gaactggaga gcatcctgct gcaacacccc
    10621 aacatcttcg acgccggggt cgccggcctg cccgacgacg atgccggcga gctgcccgcc
    10681 gcagtcgtcg tgctggaaca cggtaaaacc atgaccgaga aggagatcgt ggactatgtg
    10741 gccagccagg ttacaaccgc caagaagctg cgcggtggtg ttgtgttcgt ggacgaggtg
    10801 cctaaaggac tgaccggcaa gttggacgcc cgcaagatcc gcgagattct cattaaggcc
    10861 aagaagggcg gcaagatcgc cgtgtaagaa ttcgatatca agcttatcgg taatcaacct
    10921 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
    10981 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
    11041 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
    11101 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
    11161 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
    11221 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
    11281 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
    11341 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
    11401 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
    11461 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
    11521 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
    11581 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
    11641 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
    11701 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
    11761 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
    11821 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
    11881 gccaatgaag gagagaacac ccgctt
//
