LOCUS       pHCMV_ABLV_G            6339 bp    DNA     circular SYN 29-JUL-2023
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.0.
FEATURES             Location/Qualifiers
     misc_feature    join(6324..6339,1..14)
                     /note="pLannotate"
                     /standard_name="MCS (fragment)"
     regulatory      97..476
                     /note="Geneious type: enhancer"
                     /note="pLannotate"
                     /standard_name="CMV enhancer"
     regulatory      477..680
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="CMV promoter"
     intron          792..1364
                     /note="pLannotate"
                     /standard_name="β-globin intron"
     CDS             1425..2999
                     /note="pLannotate"
                     /standard_name="G"
     misc_feature    3109..3164
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /standard_name="β-globin poly(A) signal"
     protein_bind    complement(3546..3562)
                     /note="pLannotate"
                     /standard_name="lac operator"
     regulatory      complement(3570..3600)
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="lac promoter"
     protein_bind    complement(3615..3636)
                     /note="pLannotate"
                     /standard_name="CAP binding site"
     CDS             complement(3649..3741)
                     /note="pLannotate"
                     /standard_name="lacI (fragment)"
     rep_origin      complement(3924..4512)
     CDS             complement(4683..5543)
                     /note="pLannotate"
                     /standard_name="AmpR"
     regulatory      complement(5544..5648)
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="AmpR promoter"
     rep_origin      complement(5701..6129)
     CDS             complement(6134..6289)
                     /note="pLannotate"
                     /standard_name="lacZα (fragment)"
     regulatory      6297..6315
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="T7 promoter"
ORIGIN      
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgctg ctgcaagtga
     1441 ttctgctggt gagcctcacc gctattctgc cttgcaccgg ccagttccct ctgtacgcta
     1501 ttcccgacaa gctcggacct tggagcccca tcgacattca tcatctgagc tgccccaata
     1561 acctcattgt ggaggatgag ggctgcacat ccctctccgg atttagctac atggagctga
     1621 aggtgggctt tatcaccacc atcaaggtct ccggattcac atgcaccgga gtcgtgacag
     1681 aatccgagac ctacacaaac ttctttggct acgtgaccac caccttcaag aggaagcact
     1741 tcagacctac ccccgagagc tgtagaaagg cttacaactg gaagatcgct ggcgacccta
     1801 gatacgaaga atctctgcat aatccctacc ccgattacca ttggctgaga acagtgacca
     1861 ccacaaagga gagcctcctc atcatctccc ccagcgtggt ggatatggat ccctacgaca
     1921 agtctctgca ctctagaatg tttcctaaag gcagctgcag cggcgcttcc atccctagcg
     1981 tgttctgttc caccaaccac gactatacac tgtggatgcc cgaggacagc aactccggca
     2041 tgagctgcga catcttcacc atgtccaagg gcaagaaagc ttccaagggc ggcaaggtgt
     2101 gtggcttcgt ggacgaaaga ggactgtaca agtctctgaa gggcgcttgc aaactgaagc
     2161 tctgcggcat cagcggactg agactgctgg acggcagctg ggtgtccatc cagaaccacg
     2221 aggaggtgaa atggtgtagc cctaaccagc tcgtcaacat ccacgacttt aacgccgacg
     2281 agattgagca cctcatcgtg gaagagctca tcaaggagag agaggagtgc ctcgatgctc
     2341 tcgagtccat catcacaacc aagtctgtga gctttagaag gctgtcccat ctgagaaagc
     2401 tcgtgcccgg attcggcaag gcttacacca tcatcaacaa aacactgatg gaggccgacg
     2461 cccactacaa aagcgtgaga acatgggacg agatcatccc ttccaaggga tgcctcaagg
     2521 tgagggaaaa gtgccacccc ccttacaacg gcgtgttctt caacggcatc atcctcggcc
     2581 ccgacggcca agtcctcatt cccgaaatgc aatcctctct gctgcatcag catacagagc
     2641 tgctcgagtc ctccgtgatc cctctgattc accctctcgc cgatcccagc accatcttta
     2701 gaggagatga cgaggctgag ggctttatcg aagtccatct gcccgacatc caaaagcaag
     2761 tgtccggcat tgatctggga ctgtccgagt gggagaggta tctgatcatt ggcatcagcg
     2821 ccatcattct gttcattctg gccattattt tcacaatctg ctgtagaagg tgcaagagga
     2881 ggaagaagat taggaccgac cacatcgagc tcgatagaaa agtgagcgtg accagccaat
     2941 ccggaaagtc catccccagc tgggagtcct acaaatctag acaaggccat agcagaagct
     3001 gatgtcttcc tggagctcca gtcattgact ataacgtctc agatcttttt ccctctgcca
     3061 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     3121 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     3181 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     3241 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     3301 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     3361 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     3421 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     3481 tatggagatc cctcgacgga tcggccgcaa ttcgtaatca tgtcatagct gtttcctgtg
     3541 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3601 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3661 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3721 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3781 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3841 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3901 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3961 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4021 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4081 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4141 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4201 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4261 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4321 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4381 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4441 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4501 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4561 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4621 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4681 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4741 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4801 cccagtgctg caatgatacc gcgagaccca cgctcaccgg caccagattt atcagcaata
     4861 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4921 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4981 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5041 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5101 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5161 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5221 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5281 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5341 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5401 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5461 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5521 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5581 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5641 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     5701 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     5761 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     5821 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     5881 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     5941 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     6001 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     6061 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     6121 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     6181 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     6241 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     6301 acgactcact atagggcgaa ttggagctcc accgcggtg
//
