LOCUS       pHCMV_GP64              6300 bp ds-DNA     circular     04-AUG-2023
DEFINITION  .
ACCESSION   Y14837
VERSION     Y14837.1  GI:2440162
KEYWORDS    .
SOURCE      Cloning vector pUC57.
  ORGANISM  Cloning vector pUC57 other sequences; artificial sequences;
            vectors.
COMMENT     Imported using the Genbank importer. File name: CHIKV_E1E2 in      
            pHCMV_Library_final.seq Cloning vector pUC57, complete sequence.
FEATURES             Location/Qualifiers
     regulatory      97..476
                     /note="Geneious type: enhancer"
                     /note="simian cytomegalovirus major immediate early
                     transcription unit IE94 promoter region"
                     /regulatory_class="enhancer"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV enhancer"
     regulatory      477..680
                     /note="Geneious type: promoter"
                     /note="cytomegalovirus (CMV) enhancer sequence"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CMV"
     intron          792..1364
                     /note="Intron 2 of rabbit gene for beta globin - enhancer
                     of transgene expression"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bGlob"
     CDS             1425..2963
                     /translation="MVSAIVLYVLLAAAAHSAFAAEHCNAQMKTGPYKIKNLDITPPK
                     ETLQKDVEITIVETDYNENVIIGYKGYYQAYAYNGGSLDPNTRVEETMKTLNVGKEDL
                     LMWSIRQQCEVGEELIDRWGSDSDDCFRDNEGRGQWVKGKELVKRQNNNHFAHHTCNK
                     SWRCGISTSKMYSRLECQDDTDECQVYILDAEGNPINVTVDTVLHRDGVSMILKQKST
                     FTTRQIKAACLLIKDDKNNPESVTREHCLIDNDIYDLSKNTWNCKFNRCIKRKVEHRV
                     KKRPPTWRHNVRAKYTEGDTATKGDLMHIQEELMYENDLLKMNIELMHAHINKLNNML
                     HDLIVSVAKVDERLIGNLMNNSVSSTFLSDDTFLLMPCTNPPAHTSNCYNNSIYKEGR
                     WVANTDSSQCIDFSNYKELAIDDDVEFWIPTIGNTTYHDSWKDASGWSFIAQQKSNLI
                     TTMENTKFGGVGTSLSDITSMAEGELAAKLTSFMFGHVVNFVIILIVILFLYCMIRNR
                     NRQY*"
                     /standard_name="Translation 1425-2963"
     misc_feature    1425..2963
                     /standard_name="GP64"
     misc_feature    1425..1484
                     /standard_name="SecretionSignal"
     misc_feature    complement(1474..1488)
                     /note="polyarginine tag used for protein purification (may
                     sometime have 6 R residues instead of 5)"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="polyArg"
     misc_feature    2865..2939
                     /standard_name="TM"
     misc_feature    3507..3523
                     /note="Lac operon operator"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lacO"
     regulatory      complement(3531..3561)
                     /note="Geneious type: promoter"
                     /note="promoter sequence of the lac operon"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="lac"
     misc_feature    3539..3550
                     /note="C-tag used for protein purification"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on protein similarity"
                     /standard_name="C"
     regulatory      3576..3597
                     /note="catabolite activator protein binding site"
                     /regulatory_class="other"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="CAP BS"
     rep_origin      complement(3885..4473)
     CDS             complement(4644..5504)
                     /note="confers ampicillin resistance"
                     /product="beta-lactamase"
                     /gene="bla"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ampR"
     regulatory      complement(5505..5609)
                     /note="Geneious type: promoter"
                     /note="Promoter for ampicillin resistance gene
                     (b-lactamase)"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="amp"
     rep_origin      complement(5635..6090)
     regulatory      6258..6275
                     /note="Geneious type: promoter"
                     /note="T7 RNA polymerase promoter"
                     /regulatory_class="promoter"
                     /note="Derived using Geneious Prime 2023.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="T7"
ORIGIN      
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatggtc agcgccatcg
     1441 tgctgtatgt gctgcttgcc gctgctgctc acagcgcctt cgccgccgag cactgcaatg
     1501 cccagatgaa gaccggcccc tacaagatca aaaatctgga catcacccct cctaaggaaa
     1561 ccctgcagaa agatgtggaa atcacaatcg tcgagacaga ctacaatgag aacgtgatca
     1621 tcggctacaa gggctactac caggcctacg cctataacgg cggaagcctg gatcctaata
     1681 caagagtgga ggaaaccatg aaaaccctga acgtgggcaa ggaggacctg ttgatgtgga
     1741 gcatccggca acagtgcgag gtgggagagg aactgatcga tagatggggc agcgactctg
     1801 atgactgctt cagagacaac gagggcagag gccagtgggt gaaaggaaag gagctggtga
     1861 agagacaaaa caacaaccac ttcgcccacc acacctgcaa caagtcttgg cggtgcggca
     1921 tcagcacctc caaaatgtat tctagactgg aatgccagga cgataccgat gagtgccagg
     1981 tgtacatcct ggacgccgag ggcaacccca tcaacgttac agtggacacc gtgctgcaca
     2041 gagacggcgt ttccatgatc ctcaagcaga aaagcacctt taccacccgc cagatcaagg
     2101 ccgcatgcct gctgattaag gacgacaaga acaatcctga gtccgtgacc cgggaacatt
     2161 gtctgattga taacgacatc tacgacctga gcaagaacac ctggaactgc aagttcaacc
     2221 ggtgcatcaa gaggaaggtg gagcacagag tgaagaagcg gccccccacc tggagacaca
     2281 acgtgcgggc caaatacacc gagggcgata ccgccaccaa gggagatctg atgcacatcc
     2341 aggaggaact gatgtacgag aacgacctgc tgaagatgaa tatcgagctg atgcacgccc
     2401 acatcaacaa gctgaataac atgctgcacg acctgatcgt gtcagtggcc aaggtggacg
     2461 agagactgat cggcaatctg atgaacaaca gcgtgagttc taccttcctg agcgacgata
     2521 catttctcct gatgccttgt acaaaccctc cagctcatac aagcaattgt tacaacaata
     2581 gcatctacaa ggaaggaaga tgggtcgcca acacagacag cagccagtgt atcgacttca
     2641 gcaactacaa ggaactggcc atcgacgacg acgtggaatt ctggatccct accatcggca
     2701 acaccaccta ccacgatagc tggaaggacg ccagcggctg gtccttcatc gcccagcaga
     2761 aaagcaacct gatcacaaca atggaaaaca ccaagttcgg cggcgtgggt acatctctgt
     2821 ctgatattac aagcatggcc gagggcgagc tggccgctaa gctgaccagc tttatgttcg
     2881 gccacgtggt gaacttcgtg atcatcctga tcgtgatcct gttcctgtac tgcatgatca
     2941 gaaacagaaa ccggcagtac tagtgtcttc ctggagctcc agtcattgac tataacgtct
     3001 cagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
     3061 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
     3121 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
     3181 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa
     3241 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
     3301 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     3361 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     3421 tcatagctgt ccctcttctc ttatggagat ccctcgacgg atcggccgca attcgtaatc
     3481 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     3541 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     3601 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     3661 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     3721 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3781 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4321 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4441 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4501 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4561 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4621 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4681 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4741 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4801 gcaccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4861 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4921 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     4981 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5041 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5101 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5161 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5221 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     5281 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5341 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5401 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5461 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5521 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5581 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg
     5641 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     5701 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     5761 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     5821 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     5881 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     5941 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     6001 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     6061 ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt
     6121 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     6181 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     6241 cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggagctc caccgcggtg
//
