LOCUS       pHCMV_ISFV_G            6333 bp    DNA     circular SYN 29-JUL-2023
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.0.
FEATURES             Location/Qualifiers
     misc_feature    join(6318..6333,1..14)
                     /note="pLannotate"
                     /standard_name="MCS (fragment)"
     regulatory      97..476
                     /note="Geneious type: enhancer"
                     /note="pLannotate"
                     /standard_name="CMV enhancer"
     regulatory      477..680
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="CMV promoter"
     intron          792..1364
                     /note="pLannotate"
                     /standard_name="β-globin intron"
     CDS             1425..2993
                     /note="pLannotate"
                     /standard_name="G"
     misc_feature    3103..3158
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /standard_name="β-globin poly(A) signal"
     protein_bind    complement(3540..3556)
                     /note="pLannotate"
                     /standard_name="lac operator"
     regulatory      complement(3564..3594)
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="lac promoter"
     protein_bind    complement(3609..3630)
                     /note="pLannotate"
                     /standard_name="CAP binding site"
     CDS             complement(3643..3735)
                     /note="pLannotate"
                     /standard_name="lacI (fragment)"
     rep_origin      complement(3918..4506)
     CDS             complement(4677..5537)
                     /note="pLannotate"
                     /standard_name="AmpR"
     regulatory      complement(5538..5642)
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="AmpR promoter"
     rep_origin      complement(5695..6123)
     CDS             complement(6128..6283)
                     /note="pLannotate"
                     /standard_name="lacZα (fragment)"
     regulatory      6291..6309
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="T7 promoter"
ORIGIN      
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgacc agcgtgctgt
     1441 ttatggtggg agtgctgctg ggcgcttttg gcagcaccca ctgctccatc cagatcgtgt
     1501 tccctagcga aacaaagctg gtctggaaac ccgtgctgaa aggcaccaga tattgccccc
     1561 agagcgccga gctcaatctg gaacccgatc tcaaaaccat ggccttcgac tccaaggtgc
     1621 ccatcggcat tacacccagc aacagcgacg gctatctctg ccacgccgcc aaatgggtga
     1681 caacatgcga ctttagatgg tacggcccca agtatatcac acacagcgtg cattctctga
     1741 gacctacagt gagcgactgt aaggctgccg tggaggccta taacgctggc acactgatgt
     1801 accccggatt ccctcccgag agctgcggct acgccagcat caccgacagc gagttctatg
     1861 tgatgctggt cacccctcac cccgtgggag tcgacgatta tagaggacac tgggtcgacc
     1921 ctctcttccc cacatccgag tgcaacagca atttctgcga gacagtccac aacgccacca
     1981 tgtggatccc caaggacctc aaaacccacg atgtctgctc ccaagacttc cagaccatta
     2041 gggtcagcgt catgtaccct cagacaaagc ccaccaaagg cgctgatctg accctcaaaa
     2101 gcaagttcca cgctcacatg aagggcgata gagtgtgcaa gatgaagttc tgtaacaaga
     2161 acggactgag actcggaaac ggagagtgga tcgaggtggg agatgaggtg atgctggata
     2221 actccaagct gctgtctctg tttcccgact gtctggtggg cagcgtcgtc aaatccacac
     2281 tcctctccga gggcgtgcag accgctctgt gggagacaga cagactgctg gactactctc
     2341 tgtgccaaaa cacatgggag aagatcgata gaaaggaacc tctgtccgct gtggatctgt
     2401 cctacctcgc ccctagatcc cccggcaaag gaatggccta catcgtcgcc aacggaagcc
     2461 tcatgagcgc tcccgctaga tacattaggg tctggattga cagccccatt ctgaaggaga
     2521 tcaaaggcaa gaaggaaagc gccagcggca tcgacacagt gctgtgggaa cagtggctgc
     2581 ccttcaatgg catggaactc ggccctaatg gactgatcaa gaccaagagc ggctacaagt
     2641 ttcctctgta tctgctgggc atgggaatcg tggaccaaga tttgcaagag ctgagcagcg
     2701 tcaatcccgt ggaccacccc catgtgccca tcgcccaagc tttcgtgagc gagggagagg
     2761 aggtgttctt cggcgatacc ggagtgagca agaaccccat cgagctcatc agcggatggt
     2821 tcagcgactg gaaagagacc gctgccgctc tgggattcgc tgccatcagc gtgatcctca
     2881 tcatcggact gatgagactg ctgcctctgc tgtgtagaag aagaaagcag aagaaagtga
     2941 tctacaagga cgtggagctc aacagcttcg accccagaca agccttccat aggtgatgtc
     3001 ttcctggagc tccagtcatt gactataacg tctcagatct ttttccctct gccaaaaatt
     3061 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     3121 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     3181 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     3241 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     3301 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     3361 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     3421 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     3481 gatccctcga cggatcggcc gcaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     3541 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3601 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     3661 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3721 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3781 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3841 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3901 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3961 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4021 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4081 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4141 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4201 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4261 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4321 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4381 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4441 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4501 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4561 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4621 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4681 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4741 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4801 gctgcaatga taccgcgaga cccacgctca ccggcaccag atttatcagc aataaaccag
     4861 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4921 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4981 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5041 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5101 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5161 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5221 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5281 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5341 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5401 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5461 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5521 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5581 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5641 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     5701 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     5761 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     5821 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     5881 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     5941 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     6001 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     6061 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     6121 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     6181 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     6241 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     6301 cactataggg cgaattggag ctccaccgcg gtg
//
