LOCUS       pHCMV_JUNV_GPC          6219 bp    DNA     circular SYN 29-JUL-2023
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.0.
FEATURES             Location/Qualifiers
     misc_feature    join(6204..6219,1..14)
                     /note="pLannotate"
                     /standard_name="MCS (fragment)"
     regulatory      97..476
                     /note="Geneious type: enhancer"
                     /note="pLannotate"
                     /standard_name="CMV enhancer"
     regulatory      477..680
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="CMV promoter"
     intron          792..1364
                     /note="pLannotate"
                     /standard_name="β-globin intron"
     CDS             1425..2879
                     /note="pLannotate"
                     /standard_name="GPC"
     misc_feature    2989..3044
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /standard_name="β-globin poly(A) signal"
     protein_bind    complement(3426..3442)
                     /note="pLannotate"
                     /standard_name="lac operator"
     regulatory      complement(3450..3480)
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="lac promoter"
     protein_bind    complement(3495..3516)
                     /note="pLannotate"
                     /standard_name="CAP binding site"
     CDS             complement(3529..3621)
                     /note="pLannotate"
                     /standard_name="lacI (fragment)"
     rep_origin      complement(3804..4392)
     CDS             complement(4563..5423)
                     /note="pLannotate"
                     /standard_name="AmpR"
     regulatory      complement(5424..5528)
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="AmpR promoter"
     rep_origin      complement(5581..6009)
     CDS             complement(6014..6169)
                     /note="pLannotate"
                     /standard_name="lacZα (fragment)"
     regulatory      6177..6195
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="T7 promoter"
ORIGIN      
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatggga cagttcatca
     1441 gctttatgca agagattccc acatttctgc aagaggctct gaatattgcc ctcgtcgccg
     1501 tgtctctgat tgccatcatc aagggcatcg tcaatctgta caagagcgga ctgtttcagt
     1561 tctttgtgtt tctggccctc gccggaagaa gctgtaccga ggaagccttc aaaatcggac
     1621 tgcataccga gttccagacc gtcagcttct ccatggtcgg cctcttcagc aacaatcctc
     1681 acgacctccc tctgctgtgt acactgaaca agagccacct ctacatcaag ggaggcaacg
     1741 cctccttcca gatcagcttt gacgacattg ccgtgctgct gcctcaatac gacgtcatca
     1801 ttcaacaccc cgccgatatg agctggtgct ccaaaagcga cgaccagatt tggctctccc
     1861 agtggttcat gaacgctgtc ggccacgatt ggcatctgga tccccctttt ctgtgcagaa
     1921 atagaaccaa gaccgagggc ttcatcttcc aagtgaacac cagcaagacc ggcgtgaacg
     1981 aaaactatgc caagaagttc aagaccggaa tgcaccatct gtacagagaa taccccgaca
     2041 gctgccccaa tggcaagctg tgtctgatga aggctcagcc caccagctgg cctctgcagt
     2101 gtcctctgga tcacgtgaat acactgcact tcctcacaag aggaaagaac attcagctgc
     2161 ctaggaggtc tctgaaggcc tttttcagct ggtctctgac agacagcagc ggaaaagaca
     2221 cccccggcgg ctattgtctc gaggagtgga tgctggtcgc cgctaagatg aaatgcttcg
     2281 gcaataccgc cgtggccaaa tgtaatctga accacgactc cgagttctgt gatatgctga
     2341 ggctgttcga ctacaacaag aacgctatca agacactgaa cgacgagacc aagaagcaag
     2401 tgaatctgat gggccaaacc attaacgctc tgatcagcga taatctgctc atgaaaaata
     2461 agattagaga gctcatgagc gtgccttatt gcaactacac caaattctgg tacgtgaacc
     2521 acacactgag cggacaacac tctctgccta ggtgctggct gatcaagaac aactcctatc
     2581 tcaatattag cgactttaga aacgactgga tcctcgagag cgactttctg atcagcgaga
     2641 tgctcagcaa ggagtatagc gataggcaag gcaagacacc tctcacactg gtggacatct
     2701 gcttctggtc caccgtgttc ttcacagcca gcctctttct gcatctggtg ggcatcccca
     2761 cacatagaca tattagagga gaagcttgcc ctctgcctca tagactcaac tctctgggag
     2821 gatgtagatg cggaaaatac cccaatctga aaaagcccac cgtgtggaga agaggacatt
     2881 gatgtcttcc tggagctcca gtcattgact ataacgtctc agatcttttt ccctctgcca
     2941 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
     3001 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
     3061 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
     3121 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
     3181 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
     3241 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
     3301 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
     3361 tatggagatc cctcgacgga tcggccgcaa ttcgtaatca tgtcatagct gtttcctgtg
     3421 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     3481 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     3541 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     3601 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     3661 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     3721 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     3781 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     3841 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     3901 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3961 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4021 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4081 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4141 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4201 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4261 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4321 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4381 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4441 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4501 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4561 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4621 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4681 cccagtgctg caatgatacc gcgagaccca cgctcaccgg caccagattt atcagcaata
     4741 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4801 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4861 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4921 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4981 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5041 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5101 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5161 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     5221 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     5281 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     5341 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5401 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5461 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5521 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa
     5581 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca
     5641 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga
     5701 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc
     5761 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
     5821 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
     5881 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
     5941 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
     6001 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
     6061 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
     6121 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcgtaat
     6181 acgactcact atagggcgaa ttggagctcc accgcggtg
//
