LOCUS       pHCMV_PICV_GPC          6273 bp    DNA     circular SYN 29-JUL-2023
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.0.
FEATURES             Location/Qualifiers
     misc_feature    join(6258..6273,1..14)
                     /note="pLannotate"
                     /standard_name="MCS (fragment)"
     regulatory      97..476
                     /note="Geneious type: enhancer"
                     /note="pLannotate"
                     /standard_name="CMV enhancer"
     regulatory      477..680
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="CMV promoter"
     intron          792..1364
                     /note="pLannotate"
                     /standard_name="β-globin intron"
     CDS             1425..2933
                     /note="pLannotate"
                     /standard_name="GPC"
     misc_feature    3043..3098
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /standard_name="β-globin poly(A) signal"
     protein_bind    complement(3480..3496)
                     /note="pLannotate"
                     /standard_name="lac operator"
     regulatory      complement(3504..3534)
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="lac promoter"
     protein_bind    complement(3549..3570)
                     /note="pLannotate"
                     /standard_name="CAP binding site"
     CDS             complement(3583..3675)
                     /note="pLannotate"
                     /standard_name="lacI (fragment)"
     rep_origin      complement(3858..4446)
     CDS             complement(4617..5477)
                     /note="pLannotate"
                     /standard_name="AmpR"
     regulatory      complement(5478..5582)
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="AmpR promoter"
     rep_origin      complement(5635..6063)
     CDS             complement(6068..6223)
                     /note="pLannotate"
                     /standard_name="lacZα (fragment)"
     regulatory      6231..6249
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="T7 promoter"
ORIGIN      
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgggc cagattgtga
     1441 ccctcatcca aagcattccc gaagtgctgc aagaggtgtt caacgtggct ctgatcatcg
     1501 tcagcgtgct gtgcatcgtg aagggcttcg tcaatctgat gaggtgtggc ctcttccagc
     1561 tcgtcacctt cctcattctg agcggaagaa gctgcgacag catgatgatc gacagaaggc
     1621 acaatctgac acacgtcgag ttcaatctga caagaatgtt cgataatctg ccccagagct
     1681 gctccaagaa caatacccac cactactaca aaggccctag caataccaca tggggaatcg
     1741 agctgacact gaccaacaca agcattgcca acgagaccag cggcaatttc tccaacattg
     1801 gctctctggg ctacggaaat atcagcaact gcgataggac aagagaggct ggacacacac
     1861 tgaagtggct gctgaacgag ctccacttca acgtgctcca tgtgacaaga cacattggcg
     1921 ctagatgtaa gaccgtggag ggagctggcg tgctgatcca gtacaatctg accgtcggcg
     1981 ataggggagg agaggtggga aggcatctga ttgcctctct ggcccagatc atcggcgatc
     2041 ccaaaattgc ttgggtgggc aagtgcttca acaactgtag cggagacaca tgcagactga
     2101 ccaactgtga aggcggcacc cactacaact ttctgatcat ccagaacacc acatgggaga
     2161 accattgcac ctacacccct atggctacca ttagaatggc tctgcagaga acagcttaca
     2221 gcagcgtgtc tagaaagctc ctcggattct tcacatggga tctgtccgac agctccggac
     2281 aacacgtgcc cggcggatac tgcctcgagc aatgggccat tatctgggcc ggcatcaagt
     2341 gcttcgataa caccgtcatg gccaaatgta acaaagacca taatgaggag ttttgcgaca
     2401 ccatgaggct gttcgacttc aaccagaacg ccatcaagac actgcagctg aacgtcgaga
     2461 actctctgaa tctgtttaaa aagaccatta acggcctcat cagcgactct ctggtgatca
     2521 gaaactctct gaagcagctg gccaagatcc cttactgtaa ctataccaaa ttttggtata
     2581 ttaacgatac catcaccggc agacactctc tgccccagtg ttggctggtg cacaacggaa
     2641 gctatctgaa tgagacccac ttcaaaaacg attggctctg ggaatcccag aatctgtaca
     2701 acgagatgct catgaaggag tacgaagaga ggcaaggcaa gacacctctg gctctcaccg
     2761 atatctgctt ttggtctctg gtgttctaca ccattaccgt gttcctccat attgtgggca
     2821 tccccaccca tagacatatt attggcgacg gctgccccaa accccataga atcacaagga
     2881 acagcctctg cagctgcgga tactacaagt accagaggaa tctgacaaat ggatgatgtc
     2941 ttcctggagc tccagtcatt gactataacg tctcagatct ttttccctct gccaaaaatt
     3001 atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg aaatttattt
     3061 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     3121 caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca tatgcccata
     3181 tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg aaacagcccc
     3241 ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat
     3301 tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag
     3361 ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgga
     3421 gatccctcga cggatcggcc gcaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     3481 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     3541 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     3601 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     3661 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3721 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3781 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3841 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3901 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3961 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4021 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4081 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4141 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4201 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4261 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4321 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4381 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4441 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4501 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4561 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4621 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4681 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4741 gctgcaatga taccgcgaga cccacgctca ccggcaccag atttatcagc aataaaccag
     4801 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4861 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4921 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4981 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5041 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5101 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5161 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5221 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5281 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5341 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5401 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5461 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5521 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5581 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     5641 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     5701 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     5761 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     5821 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     5881 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     5941 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     6001 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     6061 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     6121 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     6181 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     6241 cactataggg cgaattggag ctccaccgcg gtg
//
