LOCUS       pHCMV_SABV_GPC          6228 bp    DNA     circular SYN 29-JUL-2023
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.0.
FEATURES             Location/Qualifiers
     misc_feature    join(6213..6228,1..14)
                     /note="pLannotate"
                     /standard_name="MCS (fragment)"
     regulatory      97..476
                     /note="Geneious type: enhancer"
                     /note="pLannotate"
                     /standard_name="CMV enhancer"
     regulatory      477..680
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="CMV promoter"
     intron          792..1364
                     /note="pLannotate"
                     /standard_name="β-globin intron"
     CDS             1425..2888
                     /note="pLannotate"
                     /standard_name="GPC"
     misc_feature    2998..3053
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /standard_name="β-globin poly(A) signal"
     protein_bind    complement(3435..3451)
                     /note="pLannotate"
                     /standard_name="lac operator"
     regulatory      complement(3459..3489)
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="lac promoter"
     protein_bind    complement(3504..3525)
                     /note="pLannotate"
                     /standard_name="CAP binding site"
     CDS             complement(3538..3630)
                     /note="pLannotate"
                     /standard_name="lacI (fragment)"
     rep_origin      complement(3813..4401)
     CDS             complement(4572..5432)
                     /note="pLannotate"
                     /standard_name="AmpR"
     regulatory      complement(5433..5537)
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="AmpR promoter"
     rep_origin      complement(5590..6018)
     CDS             complement(6023..6178)
                     /note="pLannotate"
                     /standard_name="lacZα (fragment)"
     regulatory      6186..6204
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="T7 promoter"
ORIGIN      
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgggc caactgttct
     1441 ccttcttcga ggaagtcccc aacatcatcc acgaggccat taacatcgct ctgatcgctg
     1501 tctccctcat cgctgctctg aagggcatga tcaatctgtg gaagtccgga ctgttccagc
     1561 tgatcttctt tctcacactg gccggaagga gctgctcctt tagaattggc agatccaccg
     1621 agctgcagaa cattaccttc gacatgctca aggtgttcga ggaccaccct acaagctgca
     1681 tggtcaacca cagcacctac tacgtccacg agaacaagaa cgctacatgg tgcctcgaag
     1741 tgagcgtcac cgacgtgaca ctgctcatgg ccgaacatga cagacaagtg ctgaacaatc
     1801 tgtccaactg tgtgcatccc gccgtggaac ataggagcag aatggtcggc ctcctcgagt
     1861 ggatttttag agccctcaaa tacgatttca accacgaccc cacccctctg tgccagaagc
     1921 agaccagcac agtcaacgag acaagagtgc agatcaacat taccgaggga ttcggctccc
     1981 atggctttga ggatacaatt ctgcagaggc tgggagtgct gttcggaagc agaattgcct
     2041 tctccaacat ccaagacctc ggcaagaaga ggtttctgct gattaggaac tccacatgga
     2101 agaaccagtg cgagatgaac cacgtgaaca gcatgcacct catgctggcc aatgctggca
     2161 gaagcagcgg atctagaagg cctctgggaa tcttcagctg gaccatcacc gacgccgtgg
     2221 gaaacgacat gcccggcggc tactgcctcg agaggtggat gctcgtcacc agcgatctga
     2281 agtgctttgg caacacagct ctggccaagt gcaatctcga ccacgacagc gagttctgcg
     2341 acatgctgaa gctgtttgaa tttaacaaaa aggccattga gaccctcaac gacaacacca
     2401 agaataaggt gaatctgctg acccactcca tcaatgctct gatctccgac aatctgctga
     2461 tgaagaatag actgaaggag ctgctgaaca caccttactg caactacacc aagttttggt
     2521 acgtcaacca caccgctagc ggcgagcact ctctgcctag atgctggctg gtgagaaaca
     2581 acagctatct gaacgagagc gagtttagaa acgactggat tatcgagagc gaccatctgc
     2641 tcagcgaaat gctgaacaag gagtacattg ataggcaagg caaaacccct ctgacactgg
     2701 tcgacatctg cttttggagc acactgttct tcaccaccac actgttcctc catctggtgg
     2761 gatttcctac acatagacac attagaggcg agccttgtcc tctgccccat agactcaaca
     2821 gcagaggcgg atgcagatgc ggaaagtacc ccgagctcaa aaagcccatc acatggcata
     2881 aaaaccactg atgtcttcct ggagctccag tcattgacta taacgtctca gatctttttc
     2941 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     3001 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     3061 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     3121 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
     3181 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     3241 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     3301 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     3361 ctcttctctt atggagatcc ctcgacggat cggccgcaat tcgtaatcat gtcatagctg
     3421 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     3481 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     3541 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     3601 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     3661 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3721 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3781 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3841 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     3901 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     3961 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4021 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4081 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4141 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4201 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4261 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4321 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4381 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4441 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4501 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4561 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4621 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4681 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc accagattta
     4741 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4801 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4861 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     4921 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     4981 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5041 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5101 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5161 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5221 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5281 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5341 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5401 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5461 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5521 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     5581 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     5641 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     5701 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     5761 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     5821 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     5881 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     5941 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     6001 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     6061 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     6121 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
     6181 gcgcgtaata cgactcacta tagggcgaat tggagctcca ccgcggtg
//
