LOCUS       phcmv_vsvgss-dic        5035 bp DNA     circular SYN 29-SEP-2023
DEFINITION  .
ACCESSION   urn.local...som-beovctt
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 29, 2023 from SnapGene 7.0.2
            https://www.snapgene.com
COMMENT     Imported using the Genbank importer. File name: 
            pHCMV_VSVgSS-diCR2-10His.gb Imported using the Genbank importer. 
            File name: pHCMV_Library - ALV_Env.gb Restriction Cloning ligation 
            product.
FEATURES             Location/Qualifiers
     source          1..5035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(1738..5035,1..1417)
                     /label=source
                     /note="color: #b1ff67"
     regulatory      97..476
                     /label=Geneious type: enhancer
                     /note="color: #f8d3a9"
     regulatory      477..680
                     /label=Geneious type: promoter
                     /note="color: #f8d3a9"
     primer_bind     631..651
                     /label=primer_bind
                     /note="color: #84b0dc; direction: RIGHT"
     primer_bind     677..701
                     /label=primer_bind
                     /note="color: #84b0dc; direction: RIGHT"
     intron          792..1364
                     /label=intron
                     /note="color: #faac61"
     CDS             1425..1736
                     /codon_start=1
                     /label=Translation 1425-1769
                     /note="color: #84b0dc"
                     /translation="MKCLLYLAFLFIGVNCGTSVTCKSGDFSCGGRVNRCIPQFWRCDG
                     QVDCDNGSDEQGCPPSVTCKSGDFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPAS
                     "
     misc_feature    1425..1478
                     /label=Geneious type: ORF
                     /note="color: #b7e6d7"
     misc_feature    1425..1472
                     /label=SignalSequence
                     /note="color: #85dae9"
     misc_feature    1479..1604
                     /label=CR2
                     /note="color: #84b0dc"
     misc_feature    1605..1730
                     /label=CR2
                     /note="color: #b4abac"
     misc_feature    join(1738..5035,1..1417)
                     /label=Geneious type: Concatenated sequence
                     /note="color: #c7b0e3"
     primer_bind     complement(1740..1759)
                     /label=primer_bind
                     /note="color: #9eafd2; direction: LEFT"
     polyA_site      1805..1860
                     /label=polyA_site
                     /note="color: #d6b295"
     primer_bind     complement(1859..1878)
                     /label=primer_bind
                     /note="color: #f8d3a9; direction: LEFT"
     primer_bind     complement(2218..2234)
                     /label=primer_bind
                     /note="color: #75c6a9; direction: LEFT"
     primer_bind     complement(2231..2253)
                     /label=primer_bind
                     /note="color: #84b0dc; direction: LEFT"
     protein_bind    2242..2258
                     /label=protein_bind
                     /note="color: #b7e6d7"
     regulatory      complement(2266..2296)
                     /label=Geneious type: promoter
                     /note="color: #c7b0e3; direction: LEFT"
     protein_bind    2311..2332
                     /label=protein_bind
                     /note="color: #f58a5e"
     primer_bind     complement(2449..2466)
                     /label=primer_bind
                     /note="color: #c7b0e3; direction: LEFT"
     rep_origin      complement(2620..3208)
                     /direction=LEFT
                     /label=rep_origin
                     /note="color: #b7e6d7"
     primer_bind     complement(2700..2719)
                     /label=primer_bind
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    3538..3540
                     /label=misc_feature
                     /note="color: #ffef86"
     CDS             complement(3677..4055)
                     /codon_start=1
                     /label=CDS
                     /note="color: #84b0dc"
                     /translation="NVFQ**ALLKFCYVARYYPVLTPGKSNSVAAYTILRMTWLSTHQS
                     QKSILRMA*Q*ENYAVLP*P*VITLRPTYF*QRSEDRRS*PLFCTTWGIM*LALIVGNR
                     S*MKPYQTTSVTPRCL*QWQQR"
     primer_bind     4002..4021
                     /label=primer_bind
                     /note="color: #f8d3a9; direction: RIGHT"
     regulatory      complement(4240..4344)
                     /label=Geneious type: promoter
                     /note="color: #faac61; direction: LEFT"
     rep_origin      complement(4370..4825)
                     /direction=LEFT
                     /label=rep_origin
                     /note="color: #c6c9d1"
     primer_bind     complement(4507..4528)
                     /label=primer_bind
                     /note="color: #d6b295; direction: LEFT"
     primer_bind     4719..4738
                     /label=primer_bind
                     /note="color: #9eafd2; direction: RIGHT"
     primer_bind     4952..4974
                     /label=primer_bind
                     /note="color: #ffef86; direction: RIGHT"
     primer_bind     4966..4983
                     /label=primer_bind
                     /note="color: #b7e6d7; direction: RIGHT"
     primer_bind     4967..4983
                     /label=primer_bind
                     /note="color: #85dae9; direction: RIGHT"
     primer_bind     4993..5012
                     /label=primer_bind
                     /note="color: #faac61; direction: RIGHT"
     regulatory      4993..5011
                     /label=Geneious type: promoter
                     /note="color: #84b0dc"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatgaag tgccttttgt
     1441 acttagcctt tttattcatt ggggtgaatt gcggtacctc tgtcacctgc aaatccgggg
     1501 acttcagctg tgggggccgt gtcaaccgct gcattcctca gttctggagg tgcgatggcc
     1561 aagtggactg cgacaacggc tcagacgagc aaggctgtcc cccctctgtc acctgcaaat
     1621 ccggggactt cagctgtggg ggccgtgtca accgctgcat tcctcagttc tggaggtgcg
     1681 atggccaagt ggactgcgac aacggctcag acgagcaagg ctgtcccccc gctagcagat
     1741 ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct
     1801 ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca
     1861 ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag
     1921 agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt
     1981 catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact
     2041 tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa
     2101 ttttccttac atgttttact agccagattt ttcctcctct cctgactact cccagtcata
     2161 gctgtccctc ttctcttatg gagatccctc gacggatcgg ccgcaattcg taatcatgtc
     2221 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     2281 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     2341 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     2401 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     2461 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     2521 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     2581 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     2641 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2701 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2761 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2821 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2881 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2941 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     3001 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     3061 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     3121 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     3181 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     3241 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     3301 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     3361 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     3421 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     3481 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctgc
     3541 agatttatca gcaataaacc agccagccgg aagggcagag cgcagaagtg gtcctgcaac
     3601 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3661 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3721 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3781 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3841 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3901 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3961 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     4021 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     4081 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     4141 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     4201 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     4261 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     4321 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
     4381 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     4441 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     4501 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     4561 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     4621 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     4681 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     4741 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     4801 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
     4861 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     4921 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     4981 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg agctccaccg cggtg
//
