LOCUS       Exported                9872 bp ds-DNA     circular SYN 22-DEC-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9872)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Sep 7, 2023 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9872
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..813
                     /label=CMV promoter
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1059..1184
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1681..1914
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2417..2534
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2590..3752
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2821..3743
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3752..3776
                     /label=SSTR3 N17-FOR
     regulatory      3815..3824
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3821..4531
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4507..4551)
                     /label=Fragment.REV
     misc_feature    4566..5154
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     regulatory      4566..4954
                     /regulatory_class="other"
                     /label=WPRE
                     /note="Woodchuck hepatitis virus post-transcriptional
                     regulatory element                "
     CDS             complement(5037..5048)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5673..5853
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    5885..6109
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6155..6583
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6597..6927
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6778..6913
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6975..7022
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7041..7415
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     primer_bind     complement(7247..7265)
                     /label=Fragment.REV
     polyA_signal    7545..7666
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7715..7731)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7739..7755
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7763..7793)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7808..7829
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8117..8705)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8876..9736)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9737..9841)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1081 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1141 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1201 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1261 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1321 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1381 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1441 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1501 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     1561 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1621 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1681 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1741 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1801 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1861 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1921 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1981 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     2041 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     2101 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2161 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2221 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2281 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2341 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctacaaatgg
     2401 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2461 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2521 aaattcaaaa ttttcgggtt tattacaggg acagcagaaa ttcactttga attaattcaa
     2581 gcttcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     2641 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac
     2701 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     2761 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     2821 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     2881 ccttgaatta cttccacctg gctccagtac gtgattcttg atcccgagct ggagccaggg
     2941 gcgggccttg cgctttagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     3001 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     3061 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     3121 gtcttgtaaa tgcgggccag gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     3181 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     3241 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     3301 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     3361 gagcggaaag atggccgctt cccggccctg ctccaggggg ctcaaaatgg aggacgcggc
     3421 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aggggccttt ccgtcctcag
     3481 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     3541 ggagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     3601 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     3661 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     3721 aaagtttttt tcttccattt caggtgtcgt gaacacgcta ccggtctcga gaattctgca
     3781 gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca agggcgagga
     3841 gaataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
     3901 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg aggcctttca
     3961 gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
     4021 ccctcagttc atgtacggct ccaaggtcta cattaagcac ccagccgaca tccccgacta
     4081 cttcaagctg tccttccccg agggcttcag gtgggagcgc gtgatgaact tcgaggacgg
     4141 cggcattatt cacgttaacc aggactcctc cctgcaggac ggcgtgttca tctacaaggt
     4201 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
     4261 ctgggaggcc tccgaggagc ggatgtaccc cgaggacggc gccctgaaga gcgagatcaa
     4321 gaagaggctg aagctgaagg acggcggcca ctacgccgcc gaggtcaaga ccacctacaa
     4381 ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt tggacatcgt
     4441 gtcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     4501 caccggcggc atggacgagc tgtacaagta actagagtta acatcgaggg atcaagctta
     4561 tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4621 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4681 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     4741 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     4801 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     4861 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     4921 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     4981 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     5041 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     5101 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat
     5161 accgtcgaga cctggaaaaa catggagcaa tcacaagtag caacacagca gctaccaatg
     5221 ctgcttgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
     5281 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     5341 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
     5401 ggatctacca cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga
     5461 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg
     5521 tagaagaagc caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga
     5581 tggatgaccc ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc
     5641 acatggcccg agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag
     5701 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5761 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5821 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
     5881 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5941 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     6001 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     6061 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     6121 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     6181 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6241 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6301 ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6361 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6421 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6481 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat
     6541 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     6601 gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg
     6661 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     6721 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     6781 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6841 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     6901 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     6961 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     7021 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     7081 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     7141 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     7201 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     7261 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     7321 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     7381 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     7441 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     7501 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     7561 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7621 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     7681 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7741 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7801 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7861 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     7921 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     7981 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     8041 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     8101 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     8161 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     8221 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     8281 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg
     8341 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     8401 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     8461 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     8521 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
     8581 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     8641 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     8701 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     8761 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8821 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8881 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     8941 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     9001 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     9061 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     9121 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     9181 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     9241 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     9301 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     9361 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     9421 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     9481 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     9541 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     9601 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     9661 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     9721 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     9781 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     9841 gcacatttcc ccgaaaagtg ccacctgacg tc
//
